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Evaluation of Meloidogyne incognita and Rotylenchulus reniformis nematode-resistant cotton cultivars with supplemental Corteva Agriscience nematicides Cover

Evaluation of Meloidogyne incognita and Rotylenchulus reniformis nematode-resistant cotton cultivars with supplemental Corteva Agriscience nematicides

Open Access
|Feb 2023

Figures & Tables

Table 1

Cotton (Gossypium hirsutum) cultivars grouped into M. incognita (Mi) or R. reniformis (Rr) resistance categories by company.

CultivarMi resistanceRr resistanceCompany
Fibermax 1621 GLPRaBASF; Ludwigshafen, Germany
Fibermax 1911 GLTPRBASF; Ludwigshafen, Germany
Fibermax 2011 GTPRBASF; Ludwigshafen, Germany
Fibermax 1730 GLTPPRBASF; Ludwigshafen, Germany
Stoneville 4946 GLB2PRBASF; Ludwigshafen, Germany
Stoneville 5600 B2XFPRBASF; Ludwigshafen, Germany
Deltapine 2141 NR B3XFPRPRBayer; Leverkusen, Germany
Deltapine 2143 NR B3XFPRPRBayer; Leverkusen, Germany
Deltapine 1454NR B2RFNRbBayer; Leverkusen, Germany
Deltapine 1558NR B2RFNRBayer; Leverkusen, Germany
Deltapine 1747NR B2RFNRBayer; Leverkusen, Germany
Deltapine 1823NR B2XFNRBayer; Leverkusen, Germany
PhytoGen 250 W3FEPRCorteva AgriScience; Indianapolis, IN
PhytoGen 332 W3FERcRCorteva AgriScience; Indianapolis, IN
PhytoGen 350 W3FEPRCorteva AgriScience; Indianapolis, IN
PhytoGen 390 W3FERCorteva AgriScience; Indianapolis, IN
PhytoGen 400 W3FEPRCorteva AgriScience; Indianapolis, IN
PhytoGen 430 W3FEPRCorteva AgriScience; Indianapolis, IN
PhytoGen 440 W3FEPRCorteva AgriScience; Indianapolis, IN
PhytoGen 443 W3FERRCorteva AgriScience; Indianapolis, IN
PhytoGen 545 W3FERCorteva AgriScience; Indianapolis, IN
PhytoGen 320 W3FEPRCorteva AgriScience; Indianapolis, IN
PhytoGen 360 W3FERCorteva AgriScience; Indianapolis, IN
PhytoGen 417 WRFHRdCorteva AgriScience; Indianapolis, IN
PhytoGen 427 W3FERCorteva AgriScience; Indianapolis, IN
PhytoGen 480 W3FEHRCorteva AgriScience; Indianapolis, IN
PhytoGen 530 W3FERCorteva AgriScience; Indianapolis, IN
PhytoGen 580 W3FEHRCorteva AgriScience; Indianapolis, IN

[i] a PR are cultivars reported with partial resistant to M. incognita or R. reniformis.

b NR are cultivars reported to be resistant to M. incognita or R. reniformis.

c R are cultivars reported to have some form of resistance, but not fully evaluated yet.

d HR are cultivars reported to have high resistance with two homologous resistant genes.

Table 2

Cotton cultivar PHY 340 W3FE [susceptible(S)], PHY 360 W3FE [M. incognita resistant(R)] and PHY 332 W3FE [R. reniformis resistant (R)] and nematicide rates and combinations used in field experiments conducted at the Plant Breeding Unit (PBU), Shorter, AL and Tennessee Valley Research and Extension Center (TVREC), Belle Mina, AL in 2020 and 2021.

NumberCotton cultivarNematicides2020 rates2021 ratesApplication
1PHY 340 W3FE (S)No nematicide
2PHY 340 W3FE (S)Reklemela + Vydate C-LVb0.28 + 1.24 L/ha0.21 + 0.88 L/ haIn-furrow spray
3PHY 340 W3FE (S)Reklemel + Vydate C-LV0.56 + 2.5 L/ha0.28 + 1.24 L/ haIn-furrow spray
4PHY 340 W3FE (S)Reklemel + Vydate C-LV1.13 + 5.0 L/ha0.56 + 2.50 L/ haIn-furrow spray
5PHY 340 W3FE (S)BIOST Nematicide 100c0.026 mg ai/seed0.026 mg ai/ seedSeed treatment
6PHY 340 W3FE (S)BIOST Nematicide 100 + Reklemel + Vydate C-LV0.026 mg ai/seed + 0.28 + 1.24 L/ ha0.026 mg ai/ seed + 0.21 + 0.884 L/haSeed treatment + In-furrow spray
7PHY 340 W3FE (S)BIOST Nematicide 100 + Reklemel + Vydate C-LV0.026 mg ai/seed + 0.56 + 2.50 L/ ha0.026 mg ai/ seed + 0.28 + 1.24 L/haSeed treatment + In-furrow spray
8PHY 340 W3FE (S)BIOST Nematicide 100 + Reklemel + Vydate C-LV0.026 mg ai/seed + 1.13 + 5.0 L/ha0.026 mg ai/ seed + 1.13 + 5.0 L/haSeed treatment + In-furrow spray
9PHY 360 W3FE (R) or PHY 332 W3FE (R)cNo nematicide
10PHY 360 W3FE (R) or PHY 332 W3FE (R)Reklemel + Vydate C-LV0.28 + 1.24 L/ha0.21 + 0.88 L/ haIn-furrow spray
11PHY 360 W3FE (R) or PHY 332 W3FE (R)Reklemel + Vydate C-LV0.56 + 2.5 L/ha0.28 + 1.24 L/ haIn-furrow spray
12PHY 360 W3FE (R) or PHY 332 W3FE (R)Reklemel + Vydate C-LV1.13 + 5.0 L/ha0.56 + 2.50 L/ haIn-furrow spray
13PHY 360 W3FE (R) or PHY 332 W3FE (R)BIOST Nematicide 100e0.026 mg ai/seed0.026 mg ai/ seedSeed treatment
14PHY 360 W3FE (R) or PHY 332 W3FE (R)BIOST Nematicide 100 + Reklemel + Vydate C-LV0.026 mg ai/seed + 0.28 + 1.24 L/ ha0.026 mg ai/ seed + 0.21 + 0.88 L/haSeed treatment + In-furrow spray
15PHY 360 W3FE (R) or PHY 332 W3FE (R)BIOST Nematicide 100 + Reklemel + Vydate C-LV0.026 mg ai/seed + 0.56 + 2.50 L/ ha0.026 mg ai/ seed + 0.28 + 1.24 L/haSeed treatment + In-furrow spray
16PHY 360 W3FE (R) or PHY 332 W3FE (R)BIOST Nematicide 100 + Reklemel + Vydate C-LV0.026 mg ai/seed + 1.13 + 5.0 L/ha0.026 mg ai/ seed + 0.56 + 5.0 L/haSeed treatment + In-furrow spray

[i] a Reklemel active nematicide is 500g/L Fluazaindolizine.

b Vydate C-LV active nematicide is 452 g/L Oxamyl.

c Heat-killed Burkholderia rinojenses and its non-living spent fermentation media.

Table 3

Greenhouse trial LS means for PHY 360 W3FE, M. incognita resistant (R), PHY 332 W3FE, R. reniformis resistant (R), each paired with PHY 340 W3FE, susceptible (S) cotton variety effects on plant height, shoot fresh weight, root fresh weight, biomass, total nematode eggs, and nematodes eggs per gram of root at 30 DAP at the Plant Science Research Center, Auburn, AL in 2020 and 2021.

Cotton cultivarPlant height (cm)Shoot fresh weight (g)Root fresh weight (g)Biomass (g)Total M. incognita eggsM. incognita eggs/g of roota
PHY 360 W3FE12.63 A6.55 a4.31 b10.85 a194 b42 B
PHY 340 W3FEb11.95 ac6.21 a6.68 a12.89 a1922 a353 A
P-values0.48480.63480.00240.07490.00010.0009
Cotton cultivarPlant height (cm)Shoot fresh weight (g)Root fresh weight (g)Biomass (g)Total R. reniformis eggsR. reniformis eggs/g of root
PHY 332 W3FE11.02 A5.5 a4.57 a10.06 a447 b146 A
PHY 340 W3FE11.97 A6.24 a6.55 a12.79 a1935 a358 A
P-values0.38030.32230.07970.08710.00030.0810

[i] aData for M. incognita and R. reniformis eggs/gram of root were collected from four root systems.

bAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.

cValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly. P-values listed are type III fixed effects.

Figure 1

M. incognita PHY 340 W3FE susceptible variety on the left and the resistant PHY 360 W3FE on the right at 106 DAP.

Table 4

Field trial LS means for PHY 360 W3FE [M. incognita resistant (R)] and PHY 340 W3FE [M. incognita susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Plant Breeding Unit, Shorter, AL in 2020.

Source of variation (F-value)Plant stand (13 m row)Plant height (cm)Root fresh weight (g)Biomass (g)Lint cotton yield (kg/ha)
Cultivara0.0001****b0.54510.13380.0206*0.0402**
Nematicidec0.0001****0.13400.34120.14910.001***
Cultivar x Nematicide0.38910.92340.92040.94810.3753
Cultivar LS-means
PHY 360 W3FE (R)44.58d b13.91 a0.49 a4.57 b1621 a
PHY 340 W3FE (S)71.58 a13.69 a0.52 a5.06 a1529 b
Nematicide LS-means
No nematicide63.25 a14.13 a0.50 a4.74 a1527 ab
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)60.65 a14.79 a0.52 a5.17 a1489 ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)45.65 b13.43 a0.49 a4.22 a1363 b
Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha)48.00 b13.20 a0.54 a4.77 a1458 ab
BIOST Nematicide 100e (0.026 mg ai/seed)61.35 a12.74 a0.47 a4.41 a1657 a
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)63.45 a14.15 a0.51 a5.33 a1716 a
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)62.70 a13.74 a0.50 a4.81 a1720 a
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha)59.55 a14.25 a0.53 a5.08 a1671 a

[i] aAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.

bP-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level are indicated by *, **, ***, and ****, respectively.

cReklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.

dValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 2

Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0368. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).

Table 5

Field trial LS means for PHY 360 W3FE [M. incognita resistant (R)] and PHY 340 W3FE [M. incognita susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Plant Breeding Unit, Shorter, AL in 2021.

Source of variation (F-value)Plant stand (13 m row)Plant height (cm)Root fresh weight (g)Biomass (g)Lint yield (cotton kg/ha)
Cultivara0.0334*b0.60810.71500.0430**0.230****
Nematicidec0.20730.0001****0.0001****0.0001***0.2907***
Cultivar x Nematicide0.90260.77490.52540.74270.6664
Cultivar LS-means
PHY 360 W3FE (R)81.33d a16.88 a3.26 a42.44 a1411 a
PHY 340 W3FE (S)83.69 a16.73 a3.22 a39.31 b887 b
Nematicide LS-means
No nematicide82.25 a15.09 c2.34 d30.82 d964 B
Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha)82.35 a16.53 bc2.96 bcd37.03 cd1221 Ab
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)84.00 a16.41 bc3.18 bcd40.07 bcd1002 Ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)81.50 a17.36 ab3.41abc43.25 abc1171 Ab
BIOST Nematicide 100e (0.026 mg ai/seed)84.85 a14.94 c2.75 cd33.15 d1060 Ab
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.21 L/ha) + Vydate CLV (0.88 L/ha)82.30 a17.84 ab3.71 ab47.79 ab1143 Ab
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)83.90 a17.55 ab3.41 abc43.78 abc1316 A
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)78.90 a18.70 a4.09 a51.11 a1315 A

[i] a All seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.

b P-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level is indicated by *, **, ***, and **** respectively.

c Reklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.

d Values present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 3

Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0272. Nematicide treatments included no-nematicide control, Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha, Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.21 L/ ha) + Vydate C-LV (0.88 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.50 L/ha).

Figure 4

Auburn University’s Tennessee Valley Research Extension Center showing the nematode susceptible PHY 340 W3FE on the left and the R. reniformis-resistant PHY 332 W3FE on the right 102 DAP.

Table 6

Field trial LS means for PHY 332 W3FE [R. reniformis-resistant (R)] and PHY 340 W3FE [R. reniformis-susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Tennessee Valley Research and Extension Center, Belle Mina, AL in 2020.

Source of variation (F-value)Plant stand (13 m row)Plant height (cm)Root fresh weight (g)Biomass (g)Lint cotton yield (kg/ha)
Cultivara0.0001****b0.0139***0.0004****0.0001****0.0001****
Nematicidec0.0732*0.0001***0.0001****0.0001***0.0001****
Cultivar x Nematicide0.09660.34820.93140.94730.1298
Cultivar LS-means
PHY 332 W3FE (R)51.20d a13.42 a2.52 a21.82 A1998 b
PHY 340 W3FE (S)41.34 b12.42 b1.97 b16.62 B1284 a
Nematicide LS-means
No nematicide51.20 a11.73 c1.72 c14.32 B1289 c
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)46.85 a13.33 abc2.14 abc19.29 ab1545 ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)50.35 a14.10 a2.64 a23.70 A1732 ab
Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha)46.70 a12.63 abc1.98 bc18.13 ab1668 ab
BIOST Nematicide 100e (0.026 mg ai/seed)46.80 a12.36 bc2.1 abc18.08 ab1504 bc
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)40.20 a12.59 abc2.12 abc17.67 ab1687 ab
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)45.10 a13.45 ab2.71 a21.95 A1777 a
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha)42.95 a13.16 abc2.57 ab20.60 A1731 ab

[i] aAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.

bP-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level are indicated by *, **, ***, and ****, respectively.

cReklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.

dValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 5

Field trial R. reniformis eggs per gram of root collected from four root systems LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0178. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).

Table 7

Field trial LS means for PHY 332 W3FE [R. reniformis-resistant (R)] and PHY 340 W3FE [R. reniformis-susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Tennessee Valley Research and Extension Center, Belle Mina, AL in 2021.

Source of variation (F-value)Plant stand (13 m row)Plant height (cm)Root fresh weight (g)Biomass (g)Lint cotton yield (kg/ha)
Cultivara0.0001****b0.83510.17940.0207**0.0001****
Nematicidec0.0209**0.0001***0.0001****0.0001****0.0001****
Cultivar x Nematicide0.11230.94580.75840.78870.8616
Cultivar LS-means
PHY 332 W3FE (R)40.85d a11.22 a1.20 a12.46 A1692 a
PHY 340 W3FE (S)24.81 b12.42 a1.10 a11.07 B989 b
Nematicide LS-means
No nematicide30.07 ab9.68 c0.74 c8.07 C1008 c
Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha)39.62 a10.61 abc1.10 abcabc 10.901253 bc
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)34.26 ab11.64 ab1.30 a12.96 ab1456 ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)35.60 ab11.48 abc1.32 a12.49 ab1445 ab
BIOST Nematicide 100e (0.026 mg ai/seed)32.43 ab10.28 bc0.87 bc9.30 bc1099 c
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha)32.37 ab12.15 a1.33 a13.55 A1334 abc
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha)30.19 ab12.00 ab1.35 a14.23 A1538 ab
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha)28.10 b11.63 ab1.20 ab12.62 ab1591 a

[i] aAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.

bP-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level are indicated by *, **, ***, and ****, respectively.

cReklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.

dValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 6

Field trial R. reniformis eggs per gram of root collected from four root systems. LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0441. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).

DOI: https://doi.org/10.2478/jofnem-2023-0001 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Aug 6, 2022
Published on: Feb 17, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2023 A. Kate Turner, Scott H. Graham, Neha Potnis, Steve M. Brown, Pat Donald, Kathy S. Lawrence, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.