Table 1
Cotton (Gossypium hirsutum) cultivars grouped into M. incognita (Mi) or R. reniformis (Rr) resistance categories by company.
| Cultivar | Mi resistance | Rr resistance | Company |
|---|---|---|---|
| Fibermax 1621 GL | PRa | BASF; Ludwigshafen, Germany | |
| Fibermax 1911 GLT | PR | BASF; Ludwigshafen, Germany | |
| Fibermax 2011 GT | PR | BASF; Ludwigshafen, Germany | |
| Fibermax 1730 GLTP | PR | BASF; Ludwigshafen, Germany | |
| Stoneville 4946 GLB2 | PR | BASF; Ludwigshafen, Germany | |
| Stoneville 5600 B2XF | PR | BASF; Ludwigshafen, Germany | |
| Deltapine 2141 NR B3XF | PR | PR | Bayer; Leverkusen, Germany |
| Deltapine 2143 NR B3XF | PR | PR | Bayer; Leverkusen, Germany |
| Deltapine 1454NR B2RF | NRb | Bayer; Leverkusen, Germany | |
| Deltapine 1558NR B2RF | NR | Bayer; Leverkusen, Germany | |
| Deltapine 1747NR B2RF | NR | Bayer; Leverkusen, Germany | |
| Deltapine 1823NR B2XF | NR | Bayer; Leverkusen, Germany | |
| PhytoGen 250 W3FE | PR | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 332 W3FE | Rc | R | Corteva AgriScience; Indianapolis, IN |
| PhytoGen 350 W3FE | PR | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 390 W3FE | R | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 400 W3FE | PR | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 430 W3FE | PR | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 440 W3FE | PR | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 443 W3FE | R | R | Corteva AgriScience; Indianapolis, IN |
| PhytoGen 545 W3FE | R | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 320 W3FE | PR | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 360 W3FE | R | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 417 WRF | HRd | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 427 W3FE | R | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 480 W3FE | HR | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 530 W3FE | R | Corteva AgriScience; Indianapolis, IN | |
| PhytoGen 580 W3FE | HR | Corteva AgriScience; Indianapolis, IN |
[i] a PR are cultivars reported with partial resistant to M. incognita or R. reniformis.
b NR are cultivars reported to be resistant to M. incognita or R. reniformis.
c R are cultivars reported to have some form of resistance, but not fully evaluated yet.
d HR are cultivars reported to have high resistance with two homologous resistant genes.
Table 2
Cotton cultivar PHY 340 W3FE [susceptible(S)], PHY 360 W3FE [M. incognita resistant(R)] and PHY 332 W3FE [R. reniformis resistant (R)] and nematicide rates and combinations used in field experiments conducted at the Plant Breeding Unit (PBU), Shorter, AL and Tennessee Valley Research and Extension Center (TVREC), Belle Mina, AL in 2020 and 2021.
| Number | Cotton cultivar | Nematicides | 2020 rates | 2021 rates | Application |
|---|---|---|---|---|---|
| 1 | PHY 340 W3FE (S) | No nematicide | |||
| 2 | PHY 340 W3FE (S) | Reklemela + Vydate C-LVb | 0.28 + 1.24 L/ha | 0.21 + 0.88 L/ ha | In-furrow spray |
| 3 | PHY 340 W3FE (S) | Reklemel + Vydate C-LV | 0.56 + 2.5 L/ha | 0.28 + 1.24 L/ ha | In-furrow spray |
| 4 | PHY 340 W3FE (S) | Reklemel + Vydate C-LV | 1.13 + 5.0 L/ha | 0.56 + 2.50 L/ ha | In-furrow spray |
| 5 | PHY 340 W3FE (S) | BIOST Nematicide 100c | 0.026 mg ai/seed | 0.026 mg ai/ seed | Seed treatment |
| 6 | PHY 340 W3FE (S) | BIOST Nematicide 100 + Reklemel + Vydate C-LV | 0.026 mg ai/seed + 0.28 + 1.24 L/ ha | 0.026 mg ai/ seed + 0.21 + 0.884 L/ha | Seed treatment + In-furrow spray |
| 7 | PHY 340 W3FE (S) | BIOST Nematicide 100 + Reklemel + Vydate C-LV | 0.026 mg ai/seed + 0.56 + 2.50 L/ ha | 0.026 mg ai/ seed + 0.28 + 1.24 L/ha | Seed treatment + In-furrow spray |
| 8 | PHY 340 W3FE (S) | BIOST Nematicide 100 + Reklemel + Vydate C-LV | 0.026 mg ai/seed + 1.13 + 5.0 L/ha | 0.026 mg ai/ seed + 1.13 + 5.0 L/ha | Seed treatment + In-furrow spray |
| 9 | PHY 360 W3FE (R) or PHY 332 W3FE (R)c | No nematicide | |||
| 10 | PHY 360 W3FE (R) or PHY 332 W3FE (R) | Reklemel + Vydate C-LV | 0.28 + 1.24 L/ha | 0.21 + 0.88 L/ ha | In-furrow spray |
| 11 | PHY 360 W3FE (R) or PHY 332 W3FE (R) | Reklemel + Vydate C-LV | 0.56 + 2.5 L/ha | 0.28 + 1.24 L/ ha | In-furrow spray |
| 12 | PHY 360 W3FE (R) or PHY 332 W3FE (R) | Reklemel + Vydate C-LV | 1.13 + 5.0 L/ha | 0.56 + 2.50 L/ ha | In-furrow spray |
| 13 | PHY 360 W3FE (R) or PHY 332 W3FE (R) | BIOST Nematicide 100e | 0.026 mg ai/seed | 0.026 mg ai/ seed | Seed treatment |
| 14 | PHY 360 W3FE (R) or PHY 332 W3FE (R) | BIOST Nematicide 100 + Reklemel + Vydate C-LV | 0.026 mg ai/seed + 0.28 + 1.24 L/ ha | 0.026 mg ai/ seed + 0.21 + 0.88 L/ha | Seed treatment + In-furrow spray |
| 15 | PHY 360 W3FE (R) or PHY 332 W3FE (R) | BIOST Nematicide 100 + Reklemel + Vydate C-LV | 0.026 mg ai/seed + 0.56 + 2.50 L/ ha | 0.026 mg ai/ seed + 0.28 + 1.24 L/ha | Seed treatment + In-furrow spray |
| 16 | PHY 360 W3FE (R) or PHY 332 W3FE (R) | BIOST Nematicide 100 + Reklemel + Vydate C-LV | 0.026 mg ai/seed + 1.13 + 5.0 L/ha | 0.026 mg ai/ seed + 0.56 + 5.0 L/ha | Seed treatment + In-furrow spray |
[i] a Reklemel active nematicide is 500g/L Fluazaindolizine.
b Vydate C-LV active nematicide is 452 g/L Oxamyl.
c Heat-killed Burkholderia rinojenses and its non-living spent fermentation media.
Table 3
Greenhouse trial LS means for PHY 360 W3FE, M. incognita resistant (R), PHY 332 W3FE, R. reniformis resistant (R), each paired with PHY 340 W3FE, susceptible (S) cotton variety effects on plant height, shoot fresh weight, root fresh weight, biomass, total nematode eggs, and nematodes eggs per gram of root at 30 DAP at the Plant Science Research Center, Auburn, AL in 2020 and 2021.
| Cotton cultivar | Plant height (cm) | Shoot fresh weight (g) | Root fresh weight (g) | Biomass (g) | Total M. incognita eggs | M. incognita eggs/g of roota |
|---|---|---|---|---|---|---|
| PHY 360 W3FE | 12.63 A | 6.55 a | 4.31 b | 10.85 a | 194 b | 42 B |
| PHY 340 W3FEb | 11.95 ac | 6.21 a | 6.68 a | 12.89 a | 1922 a | 353 A |
| P-values | 0.4848 | 0.6348 | 0.0024 | 0.0749 | 0.0001 | 0.0009 |
| Cotton cultivar | Plant height (cm) | Shoot fresh weight (g) | Root fresh weight (g) | Biomass (g) | Total R. reniformis eggs | R. reniformis eggs/g of root |
| PHY 332 W3FE | 11.02 A | 5.5 a | 4.57 a | 10.06 a | 447 b | 146 A |
| PHY 340 W3FE | 11.97 A | 6.24 a | 6.55 a | 12.79 a | 1935 a | 358 A |
| P-values | 0.3803 | 0.3223 | 0.0797 | 0.0871 | 0.0003 | 0.0810 |
[i] aData for M. incognita and R. reniformis eggs/gram of root were collected from four root systems.
bAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.
cValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly. P-values listed are type III fixed effects.

Figure 1
M. incognita PHY 340 W3FE susceptible variety on the left and the resistant PHY 360 W3FE on the right at 106 DAP.
Table 4
Field trial LS means for PHY 360 W3FE [M. incognita resistant (R)] and PHY 340 W3FE [M. incognita susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Plant Breeding Unit, Shorter, AL in 2020.
| Source of variation (F-value) | Plant stand (13 m row) | Plant height (cm) | Root fresh weight (g) | Biomass (g) | Lint cotton yield (kg/ha) |
|---|---|---|---|---|---|
| Cultivara | 0.0001****b | 0.5451 | 0.1338 | 0.0206* | 0.0402** |
| Nematicidec | 0.0001**** | 0.1340 | 0.3412 | 0.1491 | 0.001*** |
| Cultivar x Nematicide | 0.3891 | 0.9234 | 0.9204 | 0.9481 | 0.3753 |
| Cultivar LS-means | |||||
| PHY 360 W3FE (R) | 44.58d b | 13.91 a | 0.49 a | 4.57 b | 1621 a |
| PHY 340 W3FE (S) | 71.58 a | 13.69 a | 0.52 a | 5.06 a | 1529 b |
| Nematicide LS-means | |||||
| No nematicide | 63.25 a | 14.13 a | 0.50 a | 4.74 a | 1527 ab |
| Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 60.65 a | 14.79 a | 0.52 a | 5.17 a | 1489 ab |
| Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 45.65 b | 13.43 a | 0.49 a | 4.22 a | 1363 b |
| Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) | 48.00 b | 13.20 a | 0.54 a | 4.77 a | 1458 ab |
| BIOST Nematicide 100e (0.026 mg ai/seed) | 61.35 a | 12.74 a | 0.47 a | 4.41 a | 1657 a |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 63.45 a | 14.15 a | 0.51 a | 5.33 a | 1716 a |
| BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 62.70 a | 13.74 a | 0.50 a | 4.81 a | 1720 a |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) | 59.55 a | 14.25 a | 0.53 a | 5.08 a | 1671 a |
[i] aAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.
bP-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level are indicated by *, **, ***, and ****, respectively.
cReklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.
dValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 2
Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0368. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).
Table 5
Field trial LS means for PHY 360 W3FE [M. incognita resistant (R)] and PHY 340 W3FE [M. incognita susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Plant Breeding Unit, Shorter, AL in 2021.
| Source of variation (F-value) | Plant stand (13 m row) | Plant height (cm) | Root fresh weight (g) | Biomass (g) | Lint yield (cotton kg/ha) |
|---|---|---|---|---|---|
| Cultivara | 0.0334*b | 0.6081 | 0.7150 | 0.0430** | 0.230**** |
| Nematicidec | 0.2073 | 0.0001**** | 0.0001**** | 0.0001*** | 0.2907*** |
| Cultivar x Nematicide | 0.9026 | 0.7749 | 0.5254 | 0.7427 | 0.6664 |
| Cultivar LS-means | |||||
| PHY 360 W3FE (R) | 81.33d a | 16.88 a | 3.26 a | 42.44 a | 1411 a |
| PHY 340 W3FE (S) | 83.69 a | 16.73 a | 3.22 a | 39.31 b | 887 b |
| Nematicide LS-means | |||||
| No nematicide | 82.25 a | 15.09 c | 2.34 d | 30.82 d | 964 B |
| Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) | 82.35 a | 16.53 bc | 2.96 bcd | 37.03 cd | 1221 Ab |
| Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 84.00 a | 16.41 bc | 3.18 bcd | 40.07 bcd | 1002 Ab |
| Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 81.50 a | 17.36 ab | 3.41abc | 43.25 abc | 1171 Ab |
| BIOST Nematicide 100e (0.026 mg ai/seed) | 84.85 a | 14.94 c | 2.75 cd | 33.15 d | 1060 Ab |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.21 L/ha) + Vydate CLV (0.88 L/ha) | 82.30 a | 17.84 ab | 3.71 ab | 47.79 ab | 1143 Ab |
| BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 83.90 a | 17.55 ab | 3.41 abc | 43.78 abc | 1316 A |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 78.90 a | 18.70 a | 4.09 a | 51.11 a | 1315 A |
[i] a All seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.
b P-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level is indicated by *, **, ***, and **** respectively.
c Reklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.
d Values present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 3
Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0272. Nematicide treatments included no-nematicide control, Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha, Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.21 L/ ha) + Vydate C-LV (0.88 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.50 L/ha).

Figure 4
Auburn University’s Tennessee Valley Research Extension Center showing the nematode susceptible PHY 340 W3FE on the left and the R. reniformis-resistant PHY 332 W3FE on the right 102 DAP.
Table 6
Field trial LS means for PHY 332 W3FE [R. reniformis-resistant (R)] and PHY 340 W3FE [R. reniformis-susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Tennessee Valley Research and Extension Center, Belle Mina, AL in 2020.
| Source of variation (F-value) | Plant stand (13 m row) | Plant height (cm) | Root fresh weight (g) | Biomass (g) | Lint cotton yield (kg/ha) |
|---|---|---|---|---|---|
| Cultivara | 0.0001****b | 0.0139*** | 0.0004**** | 0.0001**** | 0.0001**** |
| Nematicidec | 0.0732* | 0.0001*** | 0.0001**** | 0.0001*** | 0.0001**** |
| Cultivar x Nematicide | 0.0966 | 0.3482 | 0.9314 | 0.9473 | 0.1298 |
| Cultivar LS-means | |||||
| PHY 332 W3FE (R) | 51.20d a | 13.42 a | 2.52 a | 21.82 A | 1998 b |
| PHY 340 W3FE (S) | 41.34 b | 12.42 b | 1.97 b | 16.62 B | 1284 a |
| Nematicide LS-means | |||||
| No nematicide | 51.20 a | 11.73 c | 1.72 c | 14.32 B | 1289 c |
| Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 46.85 a | 13.33 abc | 2.14 abc | 19.29 ab | 1545 ab |
| Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 50.35 a | 14.10 a | 2.64 a | 23.70 A | 1732 ab |
| Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) | 46.70 a | 12.63 abc | 1.98 bc | 18.13 ab | 1668 ab |
| BIOST Nematicide 100e (0.026 mg ai/seed) | 46.80 a | 12.36 bc | 2.1 abc | 18.08 ab | 1504 bc |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 40.20 a | 12.59 abc | 2.12 abc | 17.67 ab | 1687 ab |
| BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 45.10 a | 13.45 ab | 2.71 a | 21.95 A | 1777 a |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) | 42.95 a | 13.16 abc | 2.57 ab | 20.60 A | 1731 ab |
[i] aAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.
bP-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level are indicated by *, **, ***, and ****, respectively.
cReklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.
dValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 5
Field trial R. reniformis eggs per gram of root collected from four root systems LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0178. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).
Table 7
Field trial LS means for PHY 332 W3FE [R. reniformis-resistant (R)] and PHY 340 W3FE [R. reniformis-susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Tennessee Valley Research and Extension Center, Belle Mina, AL in 2021.
| Source of variation (F-value) | Plant stand (13 m row) | Plant height (cm) | Root fresh weight (g) | Biomass (g) | Lint cotton yield (kg/ha) |
|---|---|---|---|---|---|
| Cultivara | 0.0001****b | 0.8351 | 0.1794 | 0.0207** | 0.0001**** |
| Nematicidec | 0.0209** | 0.0001*** | 0.0001**** | 0.0001**** | 0.0001**** |
| Cultivar x Nematicide | 0.1123 | 0.9458 | 0.7584 | 0.7887 | 0.8616 |
| Cultivar LS-means | |||||
| PHY 332 W3FE (R) | 40.85d a | 11.22 a | 1.20 a | 12.46 A | 1692 a |
| PHY 340 W3FE (S) | 24.81 b | 12.42 a | 1.10 a | 11.07 B | 989 b |
| Nematicide LS-means | |||||
| No nematicide | 30.07 ab | 9.68 c | 0.74 c | 8.07 C | 1008 c |
| Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) | 39.62 a | 10.61 abc | 1.10 abc | abc 10.90 | 1253 bc |
| Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 34.26 ab | 11.64 ab | 1.30 a | 12.96 ab | 1456 ab |
| Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 35.60 ab | 11.48 abc | 1.32 a | 12.49 ab | 1445 ab |
| BIOST Nematicide 100e (0.026 mg ai/seed) | 32.43 ab | 10.28 bc | 0.87 bc | 9.30 bc | 1099 c |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) | 32.37 ab | 12.15 a | 1.33 a | 13.55 A | 1334 abc |
| BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) | 30.19 ab | 12.00 ab | 1.35 a | 14.23 A | 1538 ab |
| BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) | 28.10 b | 11.63 ab | 1.20 ab | 12.62 ab | 1591 a |
[i] aAll seeds were treated with Metalaxyl 4.0 ST, Fludioxonil 4L ST, Myclobutanil 240 ST, Resonate 600.
bP-values for Type III fixed effects with significance at the 0.1, 0.05, 0.01, and 0.001 level are indicated by *, **, ***, and ****, respectively.
cReklemel and Vydate C-LV were applied at planting as an in-furrow spray and BIOST Nematicide 100 was a seed treatment previously added to the seeds.
dValues present are LS-means separated using the Tukey-Kramer method at P ≤ 0.05. Values in the same column followed by the same letter do not differ significantly.

Figure 6
Field trial R. reniformis eggs per gram of root collected from four root systems. LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0441. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).