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Pratylenchus araucensis (Rhabditida: Pratylenchidae) a Widely Distributed Nematode in Musa spp. From Colombia Cover

Pratylenchus araucensis (Rhabditida: Pratylenchidae) a Widely Distributed Nematode in Musa spp. From Colombia

Journal of Nematology
Volume 54 (2022): Issue 1 (February 2022)
By: C. E. Arboleda-Riascos,  D. H. Riascos-Ortiz,  F. Varón De Agudelo,  A. T. Mosquera-Espinosa,  C. M. G. Oliveira and  J. E. Muñoz-Flórez  
Open Access
|Jan 2023

Figures & Tables

Figure 1

Pratylenchus araucensis. (A–F): Female. (A). Body of a female, (B). Anterior body region, (C and D). Vulval region. (E and F). Posterior region. (G). Posterior region of the body of a male. St = Stylet, v = Vulva, an = Anus, spc = Spicule.
Pratylenchus araucensis. (A–F): Female. (A). Body of a female, (B). Anterior body region, (C and D). Vulval region. (E and F). Posterior region. (G). Posterior region of the body of a male. St = Stylet, v = Vulva, an = Anus, spc = Spicule.

Figure 2

Biplot (separation) based on morphometric data of Pratylenchus araucensis populations. Populations of P. araucensis from Caldas, Quindío, and Valle del Cauca were assigned to their corresponding species based on morphometric data. The first two PCA axes are shown. Pa = Pratylenchus araucensis, Ara = Arauca, Cal = Caldas, CoQ = Quindío, ZaB = Zabaletas (Valle del Cauca [Buenaventura]), and DelB = Delfina (Valle del Cauca [Buenaventura]). PCA, principal component analysis.
Biplot (separation) based on morphometric data of Pratylenchus araucensis populations. Populations of P. araucensis from Caldas, Quindío, and Valle del Cauca were assigned to their corresponding species based on morphometric data. The first two PCA axes are shown. Pa = Pratylenchus araucensis, Ara = Arauca, Cal = Caldas, CoQ = Quindío, ZaB = Zabaletas (Valle del Cauca [Buenaventura]), and DelB = Delfina (Valle del Cauca [Buenaventura]). PCA, principal component analysis.

Figure 3

Phylogenetic tree obtained by maximum likelihood of the D2–D3 (LSU) partial region of species of the genus Pratylenchus. The isolates corresponding to this work are marked in bold. The numbers above nodes indicate bootstrap values >70%. LSU, large subunit.
Phylogenetic tree obtained by maximum likelihood of the D2–D3 (LSU) partial region of species of the genus Pratylenchus. The isolates corresponding to this work are marked in bold. The numbers above nodes indicate bootstrap values >70%. LSU, large subunit.

Figure 4

Phylogenetic tree obtained by maximum likelihood of the COI partial region of species of the genus Pratylenchus. The isolates corresponding to this work are marked in bold. The numbers above nodes indicate bootstrap values >70%.
Phylogenetic tree obtained by maximum likelihood of the COI partial region of species of the genus Pratylenchus. The isolates corresponding to this work are marked in bold. The numbers above nodes indicate bootstrap values >70%.

Morphometric data of studied and reference populations of Pratylenchus araucensis_

CharacteristicPlantain, Zabaletas - BuenaventuraPlantain, Delfina - BuenaventuraPlantain, CaldasPlantain, Calarcá - QuindíoBanana, Calarcá - QuíndioMusa sp. Arauca, type population (Múnera et al., 2009)
n = 19n = 14n = 19n = 15n = 10n = 40
L456 ± 24.0501.8 ±56.0484.5 ± 24.7549.2 ± 13.9551.3 ± 13.5462 ± 31
(401.2 - 496.3)(449.3-668.7)(444.4 - 538.0)(518.4-578.3)(534.7 - 570.4)(376-511)
a26.3 ±2.128.2 ± 1.929.9 ±2.025.2 ±1.324.8 ± 1.423.9 ±2.3
(20 - 29.8)(23.5-31)(23.0-32.8)(21.6-26.9)(21.6-26.3)(19.6-29.2)
b5.8 ±1.06.1 ±0.76.4 ±0.56.2 ±1.76.7 ±1.46.2 ±0.6
(4.7-7.0)(5.1 -6.9)(4.9 - 7.3)(5.1 -7.4)(5.5-7.7)(4.8 - 7.8)
b'4.4 ±0.64.7 ±0.24.3 ±0.54.1 ±0.84.5 ±1.04.7 ±0.4
(4.0-5.7)(3.7-6.0)(3.7-5.2)(3.7-5.0)(4.0 - 4.9)(3.6 - 5.9)
c17.3 ±1.218.2 ± 1.518.9± 1.318.3 ±1.017.5 ±0.417.6 ±1.4
(14.1 -22.3)(14.9-21.8)(15.2-23.4)(16.7-20.2)(16.6-18.0)(14.5-21.1)
c'2.3 ±0.52.5 ±0.42.6 ± .022.1 ±0.12.2 ±0.12.3 ±0.3
(1.4-2.8)(1.7-3.0)(1.9-3.1)(2.0-2.3)(2.1 -2.5)(1.7-2.9)
V%76.7 ±1.877.6 ± 1.180.1 ± 1.676.5 ± 1.9574.7 ±0.678 ± 1.2
(73.9-80.1)(75.7 - 79.4)(76.5 - 83.6)(72.9-81.4)(74.0 - 75.9)(75 - 80)
Stylet length12.1 ±0.612.6± 1.111.9 ±0.614.4 ±0.414.7 ±0.415.3 ±0.4
(11.1 -13.1)(10.7-14.0)(10.6-12.9)(13.5-14.9)(14.2-15.6)(14.7-15.9)
Tail length26.3 ±2.027.5 ± 1.825.7 ± 1.830.1 ±2.031.5 ±0.826.3 ±2.5
(20.9-29.6)(24.0-31.1)(22.0-29.4)(27.1 -34.6)(30.2-32.7)(21.5-31.9)
DGO2.8 ±0.62.9 ±0.52.6 ±0.42.3 ±0.12.5 ±0.12.7 ±0.4
(2.3-4.1)(2.1 -3.6)(2.0-3.2)(2.1 -2.5)(2.3-2.6)(1.8-3.1)
Maximum body16.7 ±1.517.8 ±2.015.7 ± 1.221.8 ±1.422.3 ± 1.317.0 ±1.8
diameter(13.7-19.7)(14.0-20.5)(12.3-17.2)(20.5-26.0)(21.2-24.7)(13.5-20.9)
Anal body11.1 ±0.811.6 ±0.910.2 ± 0.814.1 ±0.614.1 ± 0.611.4 ± 1.5
diameter(9.7-12.5)(10.0-13.2)(9.0 - 11.5)(13.1 -15.2)(13.2-14.8)(8.6-17.2)
Number of head2.0 ±0.02.0 ±0.02.0 ±0.02.0 ±0.02.0 ±0.0
annuli(2.0-2.0)(2.0-2.0)(2.0-2.0)(2.0-2.0)(2.0-2.0)
Lip region width6.1 ±0.57.1 ±0.96.3 ±0.58.2 ±0.48.1 ±0.37.2 ±0.3
(4.7-6.8)(5.6-9.1)(5.5 - 7.3)(7.7-8.9)(7.5-8.5)(6.7 - 7.4)
Lip region height2.5 ±0.62.8 ±0.32.5 ±0.42.1 ±0.22.4 ±0.22.5 ±0.7
(1.5-3.6)(2.3-3.2)(1.8-3.1)(1.8-2.4)(1.9-2.6)(2.0-4.0)
Pharyngeal gland35.7 ±5.331.7 ± 1.432.8 ±4.728.6 ±3.640.5 ± 1.124.2 ± 7.7
length(25.8-45.7)(30.1 -34.4)(24.0-39.2)(24.1 -33.8)(38.9-42.2)(10.4-46.6)
PUS18.5 ±5.531.2 ± 1.624.5 ±4.829.6 ±0.830.4 ± 1.218.1 ±4.0
(8.4-27.3)(29.8-33.2)(14.8-32.0)(28.6 - 31.4)(28.8-32.0)(9.8-24.5)

Correlations between the first three main components and the morphometric parameters of P_ araucensis females_

VariableMain component
Prin1Prin2Prin3
Stylet length (μm)0.2980.440-0.159
Vulvar position (%)-0.2880.4310.178
Post-uterine sac length (μm)0.3150.1760.407
Overall length0.3300.3700.245
Tail length (μm)0.379-0.0460.235
Maximum body width (μm)0.3960.005-0.182
Width body anus (μm)0.3770.133-0.188
Head region width (μm)0.242-0.3450.447
Index a (μm)-0.2940.1220.620
Index c (μm)-0.1820.547-0.097

Information of D2–D3 rDNA and COI mtDNA regions downloaded from GenBank and obtained in the present study_

Species nameLocationD2–D3 (LSU) accession numberCOI accession numberReference or source
Pratylenchus araucensisColombiaMZ636668; MZ636669 MZ636670; MZ636671 MZ636672; MZ636673 MZ636674; MZ636675 MZ636676; MZ636677 MZ636678; MZ636679 MZ636680; MZ636681 MZ636682; MZ636683MZ636684; MZ636685 MZ636686; MZ636687 MZ636688; MZ636689 MZ636690; MZ636691 MZ636692; MZ636693 MZ636694; MZ636695 MZ636696; MZ636697 MZ636698; MZ636699 MZ636700Present study
P. araucensisColombiaFJ463262; FJ463261; FJ463260; FJ463275-Múnera et al. (2009)
P. hippeastriChinaKR029084KY424099Wang et al. (2016)
P. hippeastriChinaKP161608KY424098Unpublished
P. hippeastriSouth AfricaMH324472-Shokoohi (2018)
P. hippeastriJapanKC796706; KC796707-Wujian et al. (2014)
P. scribneriUSAKX842632-Huang and Yan (2017)
P. scribneriChinaMK209593-Li et al. (2018)
P. scribneriChina-KY424093Unpublished
P. scribneriChina-KX349425Liu et al. (2016)
P. pseudocoffeaeKoreaKT175532; KT175533-Kim et al. (2016)
P. pseudocoffeaeCosta RicaKT971360-Zamora et at. (2016)
P. pseudocoffeaeChina-KY424089Unpublished
P. loosiChinaKF430796; KF430797-Unpublished
P. loosiItaly-LR215647; LR215648Unpublished
P. coffeaeChinaMN592778; MN227243; MN588282 Li et al. (2020)
P. coffeaeChina-KX349421Liu et al. (2016)
P. coffeaeChina-MN366418Unpublished
P. coffeaeUSA-KU198943; KU198942Troccoli et al. (2016)
P. speijeriChinaKF974701; KF974698; KF974697 Unpublished
P. speijeriChina KY424088; KY424087Unpublished
P. penetransUSAKY969632; KY969631 Baidoo et al. (2017)
P. penetransKorea-MN746802; MN746803; MN746801Mwamula et al. (2020)
P. kumamotoensisKoreaKT175529; KT175530; KT175525-Kim et al. (2016)
P. parazeaeChinaKP903444; KP903445; KP903443; KP903442 Wang et al. (2015)
P. parazeaeChina KY424116; KX349424; KY424115Unpublished
P. zeaeBrazilMW363009; MW363001-Abade (2020)
P. zeaeChina KX349414; KX349416; KX349419Liu et al. (2016)
P. hortiBelgium-MK114154; MK114153; MK114152Nguyen et al. (2018)
P. brachyurusChina-KX349420; KY424082; KY424083; KY424080Unpublished
DOI: https://doi.org/10.2478/jofnem-2022-0057 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Sep 16, 2022
Published on: Jan 8, 2023
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
mtDNA,
– expansion segment of LSU rDNA,
molecular biology,
morphology,
plantain
Related subjects:
Life sciences,
Life sciences, other

© 2023 C. E. Arboleda-Riascos, D. H. Riascos-Ortiz, F. Varón De Agudelo, A. T. Mosquera-Espinosa, C. M. G. Oliveira, J. E. Muñoz-Flórez, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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