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Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA Cover

Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA

Open Access
|Oct 2022

Figures & Tables

Figure 1

Photomicrographs of fresh and fixed females of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 from Virginia. (A) Entire female (fresh); (B) anterior end of fixed female; (C) vulva and tail area of fresh female; (D) tail of fixed female, and the arrow points to the anal opening; (E) esophageal area of fixed female; (F) excretory pore (arrow pointing at it) of fixed female; (G) lateral view of fixed female with four incisures, the two lines being aerolated.
Photomicrographs of fresh and fixed females of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 from Virginia. (A) Entire female (fresh); (B) anterior end of fixed female; (C) vulva and tail area of fresh female; (D) tail of fixed female, and the arrow points to the anal opening; (E) esophageal area of fixed female; (F) excretory pore (arrow pointing at it) of fixed female; (G) lateral view of fixed female with four incisures, the two lines being aerolated.

Figure 2

Phylogenetic relationships of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 isolates with closely related Telotylenchinae (Allen, 1955) Siddiqi, 2000 species. Bayesian 50% majority rule consensus tree inferred from 28S rDNA D2–D3 sequences alignment under the general time-reversible model of sequence evolution, with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). New sequences are shown in bold. PP are shown on appropriate branches. PP, posterior probabilities. GTR, general time reversible.
Phylogenetic relationships of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 isolates with closely related Telotylenchinae (Allen, 1955) Siddiqi, 2000 species. Bayesian 50% majority rule consensus tree inferred from 28S rDNA D2–D3 sequences alignment under the general time-reversible model of sequence evolution, with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). New sequences are shown in bold. PP are shown on appropriate branches. PP, posterior probabilities. GTR, general time reversible.

Figure 3

Phylogenetic relationships of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 isolates with closely related Telotylenchinae Siddiqi, 1960 species. Bayesian 50% majority rule consensus tree inferred from ITS rRNA sequence alignment under the general time reversible model of sequence evolution, with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). New sequences are shown in bold. PP are shown on appropriate branches. GTR, general time reversible; ITS, internal transcribed spacer; PP, posterior probabilities.
Phylogenetic relationships of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 isolates with closely related Telotylenchinae Siddiqi, 1960 species. Bayesian 50% majority rule consensus tree inferred from ITS rRNA sequence alignment under the general time reversible model of sequence evolution, with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). New sequences are shown in bold. PP are shown on appropriate branches. GTR, general time reversible; ITS, internal transcribed spacer; PP, posterior probabilities.

Figure 4

Phylogenetic relationships of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 isolates with closely related Telotylenchinae Siddiqi, 1960 species. Bayesian 50% majority rule consensus tree inferred from 18S rRNA sequence alignment under the general time reversible model of sequence evolution, with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). New sequences are shown in bold. PP are shown on appropriate branches. GTR, general time reversible; PP, posterior probabilities.
Phylogenetic relationships of Sauertylenchus maximus (Allen, 1955) Siddiqi, 2000 isolates with closely related Telotylenchinae Siddiqi, 1960 species. Bayesian 50% majority rule consensus tree inferred from 18S rRNA sequence alignment under the general time reversible model of sequence evolution, with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). New sequences are shown in bold. PP are shown on appropriate branches. GTR, general time reversible; PP, posterior probabilities.

Morphometrics of several populations of Sauertylenchus maximus Allen, 1955_

CharacteristicS. maximus from Virginia(this paper)S. maximusAfter Allen (1955)S. maximusAfter Yildiz et al. (2012)S. maximusAfter Geraert (2011)

Fresh female specimensFixed female specimens (vial G-4280)FemalesFemalesFemales
n691210?
L1,253.0 ± 95.3 (1,133–1,425)1,313.0 ± 60.7 (1,260–1,472)980–1,1401,094.0 ± 95.1 (932–1,210)940–1,620
A36.4 ± 2.0 (33.3–38.7)43.0 ± 1.7 (40.3–45.8)37–4747.9±4.2 (41.6–53.7)28–58
B9.1 ± 0.6 (8.3–9.9)8.0 ± 0.4 (7.5–9.0)5.4–8.17.0 ± 0.5 (6.2–8.0)
C19.4 ± 0.7 (18.6–20.8)21 ± 1 (19.4–22.3)16–2019.2 ± 1.7 (17.2–23.0)16–26
c’2.5 ± 0.2 (2.2–2.8)2.8 ± 0.2 (2.6–3.1)1.9–4.1
Stylet22 ± 1 (20–23)22 ± 1 (21–23)21.3–24.021.5 ± 0.95 (20.5–23.0)20.0–24.5
Anal body diam.26 ± 2 (22–28)23.0 ± 1.5 (21–26)18.2 ± 1.6 (15.5–20.0)
Max. body diam.34 ± 3 (30–38)30.0 ± 1.7 (27.5–33.0)22.8 ± 2.0 (20–26)23–32
Pharynx length137.5 ± 6.3 (130–150)160.0 ± 7.8 (150–172)156.0 ± 6.9 (150–70)153–192
Anterior end to excretory pore113 ± 13 (101–135)128 ± 4 (122–135)
Tail length65 ± 5 (60–75)64.0 ± 2.8 (60–70)56.8 ± 5.4 (48–65)42–83
V%50 ± 1 (49–52)52.0 ± 1.3 (50.2–54.0)52.5 ± 2.0 (48–65)47–58
Lip annules55–75–7
DOI: https://doi.org/10.2478/jofnem-2022-0041 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Submitted on: Apr 26, 2022
Published on: Oct 19, 2022
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2022 Mihail R. Kantor, Paulo Vieira, Andrea M. Skantar, Gregory Huse, Zafar A. Handoo, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.