Nematicidal Activities of Saccharin and Erythritol Against Pinewood Nematode

Kim, Junheon; Lee, Sujin

doi:10.2478/jofnem-2022-0038

Abstract

The pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner & Bührer), causes pine wilt disease (PWD) resulting in severe environmental damage to pine forest ecosystems worldwide. To develop alternative strategies for managing PWD, the nematicidal activities of two sweeteners, erythritol and saccharin, were investigated. Among these two sweeteners, saccharin induced higher mortality in a dose-dependent manner. The LC₅₀ and LC₉₀ values of saccharin were estimated to be 0.321 M and 0.615 M, respectively. However, erythritol did not exhibit nematicidal activities. The results of our study demonstrated that saccharin is lethal to PWN and shows nematicidal effects in a dose-dependent manner. Although the mechanisms of saccharin toxicity are not yet investigated, saccharin could be used as an effective alternative for the management of PWN.

References

Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18:265–267. doi/10.1093/jee/18.2.265a.
Open DOI Search in Google Scholar Back to article
Cha, D. J., Kim, J., and Soo, K. D. 2019. Nematicidal activities of three naphthoquinones against pine wood nematode, Bursaphelenchus xylophilus. Molecules 24:3634. doi/10.3390/molecules24203634.
Open DOI Search in Google Scholar Back to article
Choi, M. Y., Tang, S. B., Ahn, S. J., Amarasekare, K. G., Shearer, P. W., and Lee, J. C. 2017. Effect of non-nutritive sugars to decrease the survivorship of spotted wing drosophila, Drosophila suzukii. Journal of Insect Physiology 99:86–94. doi/10.1016/j. jinsphys.2017.04.001.
Open DOI Search in Google Scholar Back to article
Danks, H. V. 2004. Seasonal adaptations in arctic insects. Integrative and Comparative Biology 44:85– 94. doi/10.1093/icb/44.2.85.
Search in Google Scholar Back to article
Fisher, M. L., Fowler, F. E., Denning, S. S., and Watson, D. W. 2017. Survival of the house fly (Diptera: Muscidae) on truvia and other sweeteners. Journal of Medical Entomology 54:999–1005. doi/10.1093/jme/tjw241.
Open DOI Search in Google Scholar Back to article
Guo, Q., Du, G., Qi, H., Zhang, Y., Yue, T., Wang, J., and Li, R. 2017. A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode (Bursaphelenchus xylophilus). Pesticide Biochemistry and Physiology 135:64–68. doi/10.1016/j.pestbp.2016.06.003.
Open DOI Search in Google Scholar Back to article
Han, H. R., Han, B. Y., Chung, Y. J., and Shin, S. C. 2008. A simple PCR-RFLP for idenficiation of Bursaphelenchus spp. collected from Korea. The Plant Pathology Journal 24:150–163. doi/10.5423/ PPJ.2008.24.2.159.
Open DOI Search in Google Scholar Back to article
Kang, M. K., Kim, J. H., Liu, M. J., Jin, C. Z., Park, D. J., Kim, J., Sung, B. H., Kim, C. J., and Son, K. H. 2022. New discovery on the nematicidal activities of aureothin and alloaureothin isolted from endophytic bacteria, Streptomyces sp. AE170020. Scientific Reports 12:3947. doi/10.1038/s41598-022-07879-w.
Open DOI Search in Google Scholar Back to article
Korea Forest Service 2017. Guideline for the control of pine wilt disease. Daejeon, Republic of Korea: Korea Forest Service.
Search in Google Scholar Back to article
Kostal, V., Zahradnickova, H., Simek, P., and Zeleny, J. 2007. Multiple component system of sugars and polyols in the overwintering spruce bark beetle, Ips typographus. Journal of Insect Physiology 53:580– 586. doi/10.1016/j.jinsphys.2007.02.009.
Search in Google Scholar Back to article
Liu, G., Lai, D., Liu, Q. Z., Zhou, L., and Liu, Z. L. 2016. Identification of nematicidal constituents of Notopterygium incisum rhizomes against Bursaphelenchus xylophilus and Meloidogyne incognita. Molecules 21:1276. doi/10.3390/molecules21101276.
Open DOI Search in Google Scholar Back to article
Mejri, S., Magnin-Robert, M., Randoux, B., Ghinet, A., Halama, P., Siah, A., and Reignault, P. 2021. Saccharin provides protection and activates defense mechanisms in wheat against the hemibiotrophic pathogen Zymoseptoria tritici. Plant Disease 105:780– 786. doi/10.1094/PDIS-05-20-1106-RE.
Search in Google Scholar Back to article
Mota, M. M., and Vieira, P. R. 2008. Pine wilt disease: A worldwide threat to forest ecosystems. Netherlands: Springer.
Search in Google Scholar Back to article
Nakashita, H., Yoshioka, K., Yasuda, M., Nitta, T., Arai, Y., Yoshida, S., and Yamaguchi, I. 2002. Probenzazole induces systemic acquired resistance in tobacco through salicylic acid accumulation. Physiological and Molecular Plant Pathology 61:197– 203. doi/10.1006/pmpp.2002.0426.
Search in Google Scholar Back to article
O’Donnell, S., Baudier, K., and Marenda, D. R. 2016. Non-nutritive polyol sweetners differ in insecticidal activity when ingested by adult Drosophila melanogaste (Diptera: Drosophilae). Journal of Insect Science 16:1–3. doi/10.1093/jisesa/iew031.
Open DOI Search in Google Scholar Back to article
Phuong, L. T., Zhao, L., Fitrianti, A. N., Matsui, H., Noutoshi, Y., Yamamoto, M., Ichinose, Y., Shiraishi, T., and Toyoda, K. 2020. The plant activator saccharin induces resistance to wheat powdery mildew by activating multiple defense‑related genes. Journal of General Plant Pathology 86:107–113. doi/10.1007/ s10327-019-00900-7.
Open DOI Search in Google Scholar Back to article
Schmidt-Jeffris, R. A., Beers, E. H., Smytheman, P., and Rehfield-Ray, L. 2021. Erythritol, an artificial sweetener, is acaricidal against pest mites and minimally harmful to a predatory mite. Journal of Economic Entomology 114:1701–1708. doi/10.1093/ jee/toab101.
Open DOI Search in Google Scholar Back to article
Sharma, A., Reyes, J., Borgmeyer, D., Ayala-Chavez, C., Snow, K., Nuss, A., and Gulia-Nuss, M. 2020. The sugar substitute Stevia shortens the lifespan of Aedes aegypti potentially by N-linked protein glycosylation. Scientific Reports 10:6195. doi/10.1038/ s41598-020-63050-3.
Open DOI Search in Google Scholar Back to article
Silva, V., Silva, C., Soares, P., Garrido, E. M., Borges, F., and Garrido, J. 2020. Isothiazolinone biocides: Chemistry, biological, and toxicity profiles. Molecules 25:991. doi/10.3390/molecules25040991.
Open DOI Search in Google Scholar Back to article
Uchida, M., Abe, H., Sato, R., Shimura, M., and Watanabe, T. 1973. Fate of 3-allyloxy-1,2-benziothiazole 1,1,-dioxid (Oryzemate®) in rice plants. Agricultural and Biological Chemistry 37:737–745. doi/10.1271/ bbb1961.37.737.
Open DOI Search in Google Scholar Back to article
Wentz, K., Cooper, W. R., Horton, D. R., Kao, R., and Nottingham, L. B. 2020. The artificial sweetener, erythritol, has insecticidal porperties against pear psylla (Hemiptera: Psyllidae). Journal of Economic Entomology 113:2293–2299. doi/10.1093/jee/toaa124.
Open DOI Search in Google Scholar Back to article
Zamora, P., Rodríguez, V., Renedo, F., Sanz, A. V., Domínguez, J. C., Pérez-Escolar, G., Miranda, J., Ãlvarez, B., González-Casas, A., Mayor, E., Dueñas, M., Miravalles, A., Navas, A., Robertson, L., and Martín, A. B. 2015. First report of Bursaphelenchus xylophilus causing pine wilt disease on Pinus radiata in Spain. Plant Disease 99:1449. doi/10.1094/PDIS-03-15-0252-PDN.
Open DOI Search in Google Scholar Back to article
Zhang, X., Chen, S., Li, Z., and Xu, Y. 2017. Effect of sweeteners on the survival of Solenopsis invicta (Hymenoptera: Formicidae). Journal of Economic Entomology 110:593––597. doi/10.1093/jee/tox038.
Search in Google Scholar Back to article
Zhao, B. G., Futai, K., Sutherland, J. R., and Takeuchi, Y. 2008. Pine wilt disease. Japan: Springer.
Search in Google Scholar Back to article
Zheng, C., Zeng, L., and Xu, Y. 2016. Effect of sweeteners on the survival and behaviour of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Pest Management Science 72:990–996. doi/10.1002/ ps.4079.
Open DOI Search in Google Scholar Back to article

Nematicidal Activities of Saccharin and Erythritol Against Pinewood Nematode

Abstract

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