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Prevalence and Molecular Characterization of Ascaridoid Parasites of Philippine Decapterus species Cover

Prevalence and Molecular Characterization of Ascaridoid Parasites of Philippine Decapterus species

Journal of Nematology
Volume 54 (2022): Issue 1 (February 2022)
By: ,  ,  ,  ,  ,   and    
Open Access
|Aug 2022
Figures & tables

Figures & Tables

Figure 1

Fish landing area at Balayan Bay and Tayabas Bays, southern Luzon, Philippines, for fish sample collection.

Table 1

GenBank sequences of close species matches used for phylogeny construction.

Ascaridoid speciesHostHabitatGeographical OriginITS218S rRNA
Anisakis pegreffiiTrachinus radiatusMarineTunisiaMT820022-
Anisakis pegreffiiConger myriasterMarineChina-MF072697
Anisakis pegreffiiCarettaMarineItaly-EF180082
Anisakis simplexGlobicephala melaenaMarineNorwayAY826723-
Anisakis simplexScomber japonicusMarineJapanLC621351-
Anisakis simplexCarcharhinus sorrahMarineChina-MF072711
Anisakis typicaTursiops aduncusMarineEgyptHF911524-
Anisakis typicaBottlenose dolphinMarineChinaKF673776-
Anisakis typicaRastrelliger kanagurtaMarineThailandAB432909-
Anisakis typicaStenella longirostrisMarineBrazilAY826724-
Anisakis typicaKatsuwonus pelamisMarineIndonesiaKC928262-
Anisakis typicaSotalia fluviatilisMarineBrazilEU327686-
Anisakis ziphidarumPagellus bogaraveoMarinePortugalJN005767-
Anisakis ziphidarumHoplostethus cadenatiMarineMauritiusEU718473-
Anisakis ziphidarumZiphius cavirostrisMarineSouth AfricaAY826725-
Contracaecum eudyptulaeEudyptula minorMarineAustralia-EF180072
Contracaecum microcephalumNMNMNM-AY702702
Contracaecum multipapillatumNMNMNM-U94370
Hysterothylacium aduncumZoarces viviparusMarineDenmarkJX845137-
Hysterothylacium aduncumDolphin fishMarineSouth KoreaHQ702733-
Hysterothylacium aduncumLophius litulonMarineChina-MF072693
Hysterothylacium deardorffoverstreetorumParalichthys isoscelesMarineBrazil-JF718550
Hysterothylacium fortalezaeNMNMNM-U94374
Hystetothylacium reliquensNMNMNM-U94376
Hystetothylacium tetrapteriKajikia audaxMarineChina-MF072705
Hysterothylacium thalassiniPriacanthus macracanthusMarineChina-MF072702
Pseudanisakis rajaeRaja pulchraMarineChina-MF072707
Pseudoterranova decipiensGadus morhuaMarineDenmarkKM273088-
Pseudoterranova decipiensNMNMNM-U94380
Raphidascaroides brasiliensisPlatydoras costatusFreshwaterBrazil-KP726276
Raphidascaroides moraveciPlatydoras armatulusFreshwaterBrazil-KP726278
Raphidascaris acusEsox luciusFreshwater; brackish waterFinland-DQ503460
Raphidascaris lanfredaeGeophagus maximusFreshwaterBrazil-KX859077
Raphidascaris longispiculaNMMarineSouth China SeaJN102362-
Raphidascaris longispiculaUroconger lepturusMarineChinaKP326546MF072704
Raphidascaris lophiiNMNMChinaMF422212-
Raphidascaris lophiiLophius litulonMarineChinaMH211584MF072692
Raphidascaris lophiiNMMarineChinaJF809816-
Terranova caballeroiNMNMNM-U94381
aRaphidascaris (Sprentascaris) andersoniGymnogeophagus balzaniiFreshwaterBrazilMK141032-
aAscaris lumbricoidesNMNMNM-U94366

[i] aOutgroup species

ITS2, internal transcribed spacer 2; NM, not mentioned.

Figure 2

Light microscopic images of representative marine ascaridoid larvae collected from Decapterus spp. A and D, anterior part of the body, ventral view, showing the larval tooth (T), esophagus (E), and ventriculus (V); B and E (inset), detailed larval tooth (T); C and F, conical tail showing or lacking a mucron (M), respectively.

Figure 3

Prevalence (%) and mean intensity (worms/host ± SD) of marine ascaridoid larvae across the three Decapterus spp. (N = 371) collected from Tayabas Bay and Balayan Bay, southern Luzon, Philippines.

Figure 4

Prevalence (%) and mean intensity (worms/host ± SD) of marine ascaridoid larvae across the three Decapterus spp. (N = 371) collected from Tayabas Bay and Balayan Bay, southern Luzon, Philippines.

Figure 5

Aggregated distribution of marine ascaridoid larval parasites from (A) pooled Decapterus spp. samples, (B) Decapterus maruadsi, (C) Decapterus tabl, and (D) Decapterus macrosoma from Tayabas Bay and Balayan Bay, southern Luzon, Philippines.

Table 2

BLAST results for the 18S rRNA and 5.8S-ITS2-28S sequences of ascaridoid samples.

Specimen18S rRNA5.8S-ITS2-28SFamily
accession (18S/ITS2)GenBank accession% MatchGenBank accession% Match
OK659774/ OK659786MF072704 (Raphidascaris longispicula)99.84MF422212 (Raphidascaris longispicula)99.18Raphidascarididae
OK659775/ OK659787MF072711 (Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.52HF911524 (Anisakis simplex)99.88Anisakidae
OK659776/ OK659788MF072704 (Raphidascaris longispicula)99.84MF422212 (Raphidascaris longispicula)99.79Raphidascarididae
OK659777/ OK659789MF072711 (Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.68HF911524 (Anisakis simplex)99.88Anisakidae
OK659778/ OK659790MF072704 (Raphidascaris longispicula)99.52MF422212 (Raphidascaris longispicula)99.79Raphidascarididae
OK659779/ OK659791MF072704 (Raphidascaris longispicula)99.52MF422212 (Raphidascaris longispicula)99.59Raphidascarididae
OK659780/ OK659792MF072711 (Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.20HF911524 (Anisakis simplex)98.66Anisakidae
OK659781/ OK659793MF072711 (Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.68HF911524 (Anisakis simplex)98.79Anisakidae
OK659782/ OK659794MF072711(Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.52HF911524 (Anisakis simplex)99.87Anisakidae
OK659783/ OK659795MF072711(Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.68HF911524 (Anisakis simplex)98.86Anisakidae
OK659784/ OK659796MF072711 (Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.68HF911524 (Anisakis simplex)98.61Anisakidae
OK659785/ OK659797MF072711 (Anisakis simplex) MF072697 (Anisakis pegreffii) U94365 (Anisakis sp.)99.68HF911524 (Anisakis simplex)99.87Anisakidae

[i] ITS2, internal transcribed spacer 2.

Figure 6

Intra- and interspecific sequence variations in Anisakidae and Raphidascarididae obtained in the GenBank and sample isolate sequences. (A) 18S rRNA and (B) 5.8S-ITS2-28S regions.

Figure 7

ML phylogenetic tree of Anisakidae and Raphidascarididae inferred from 628 nucleotide positions of the 18S rRNA gene using the TrNef + G model of DNA substitution and rooted on Ascaris lumbricoides (U94366). Values on nodes represent ML/NJ bootstrap percentages of 1,000 bootstrap samples; values <50% are not shown. Scale bar represents five nucleotide substitutions of 1,000 nucleotides. Sequences from this study are highlighted. ML, maximum-likelihood; NJ, neighbor-joining.

Table 3

Corrected (TrNef + G) genetic distances of 18S rRNA gene and corrected (HKY + G) genetic distances of the 5.8S-ITS2-28S region of Anisakidae and Raphidascarididae nematodes.

Ascaridoid comparisonsNumber of pairwise comparisons18S rRNA5.8S-ITS2-28S region
MinimumMaximumMinimumMaximum
Intraspecific67 (18S), 118 (ITS2)00.018209800.0077422
Interspecific494 (18S), 410 (ITS2)00.042596990.004936061.8776778

[i] ITS2, internal transcribed spacer 2.

Figure 8

ML phylogenetic tree of Anisakidae and Raphidascarididae inferred from 460 nucleotide positions from the partial sequence of the 5.8S, whole sequence of the ITS2, and partial sequence of 28S rRNA gene using the HKY + G model of DNA substitution and rooted on Raphidascaris (Sprentascaris) andersoni. Values on nodes represent ML/NJ bootstrap percentages of 1,000 bootstrap samples; values <50% are not shown. Scale bar represents two nucleotide substitutions of 10 nucleotides. Sequences from this study are highlighted. ITS2, internal transcribed spacer 2; ML, maximum-likelihood.

DOI: https://doi.org/10.2478/jofnem-2022-0030 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Submitted on: Mar 25, 2021
Published on: Aug 14, 2022
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Anisakis typica,
Decapterus,
host–parasite relationship,
molecular characterization,
Raphidascaris (Ichthyascaris) lophii
Related subjects:
Life sciences,
Life sciences, other

© 2022 Tres Tinna M. Dela Cruz, Kennesa Klariz R. Llanes, Joliesa Mae S. Toledo, Junard A. Catabay, Raffy Jay C. Fornillos, Ian Kendrich C. Fontanilla, Vachel Gay V. Paller, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 54 (2022): Issue 1 (February 2022)
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