Soybean Cyst Nematode Population Development and Its Effect on Pennycress in a Greenhouse Study

Likelihood-ratio χ²-test for SCN population density, as influenced by crop ([C]: MN103, MN106, MN108, and soybean), HG type (HG Types 0, 2.5.7, and 1.3.6), and inoculation ([I]: 0, 2,000, and 20,000 eggs/100 cm³ soil).

Source of fixed variation	χ²	Numerator Df	P-value
Crop (C)	96.1514	3	< 2.2e−16^***
HG type (H)	1.4823	2	0.4766
Inoculation (I)	38.4078	1	5.74e−10^***
C × H	2.9414	6	0.8162
C × I	38.9127	3	1.81e−08^***
H × I	1.1895	2	0.5517
C × H × I	5.5155	6	0.4796

*** represents significance of the χ²-test at the 0.001 level.

Df = degrees of freedom; HG, Heterodera glycines; SCN, soybean cyst nematode.

Table 2

Two-sided t-test results of the crop treatments at inoculation level of 2,000 eggs/100 cm³ soil.

Crop	Mean	t	Df	Sig. t
MN 103	1,999	−0.003	22	0.999
MN 106	2,258	0.493	23	0.627
MN 108	1,621	−0.908	22	0.374
Soybean	9,601	4.437	23	0.0002^***

The test value was 2,000; t = t-statistic; Df = degrees of freedom.

*** represents the significance of the two-sided t-test at the 0.001 level.

Table 3

Two-sided t-test results of the crop treatments at inoculation level of 20,000 eggs/100 cm³ soil.

Crop	Mean	t	Df	Sig. t
MN 103	5,777	−11.712	20	2.09e−10^***
MN 106	6,559	−10.676	23	2.19e−10^***
MN 108	7,667	−7.738	23	7.55e−08^***
Soybean	4,0739	3.185	23	0.004^**

[i] The test value was 20,000. t = t-statistic, Df = degrees of freedom.

[ii] ^**and ^***represent significance of two-sided t-test at 0.01 and 0.001, respectively.

Table 4

Likelihood-ratio χ²-test for RF, as influenced by crop ([C]: MN103, MN106, MN108, and soybean), HG type (HG Types 0, 2.5.7, and 1.3.6), and inoculation ([I]: 0, 2,000, and 20,000 eggs/100 cm³ soil).

Source of fixed variation	χ²	Numerator Df	P-value
Crop (C)	84.963	3	< 2.2e−16^***
HG type (H)	1.2603	2	0.532513
Inoculation (I)	20.9222	1	4.78e−06^***
C × H	0.648	6	0.995546
C × I	12.5867	3	0.005621^**
H × I	0.8214	2	0.663195
C × H × I	3.7566	6	0.709577

[i] ^**and ^***represent significance of χ²-test at 0.01, and 0.001, respectively.

[ii] Df, degrees of freedom; HG, Heterodera glycines; RF, reproductive factor.

Table 5

Reproductive factor for crop treatment.

Crop	RF (P_f/P_i) at treatment level of
Crop	2,000 eggs 100/cm³	20,000 eggs 100/cm³
MN103	1.00 a	0.29 a
MN106	1.13 a	0.33 a
MN108	0.81 a	0.38 a
Susceptible soybean ‘Sturdy’	4.80 b	2.04 b

[i] The values in the same column followed by different letters denote significant differences at the P < 0.05 level using Tukey–Kramer least-square means.

[ii] P_final, egg density at experiment termination; P_initial, egg density at inoculation; RF, reproductive factor.

Table 6

Likelihood-ratio χ²-test for crop treatment biomass as influenced by inoculation ([I]: 0, 2,000, and 20,000 eggs/100 cm³ soil).

Crop	χ²	Numerator Df	P-value
MN 103	3.704	2	0.1569
MN 106	0.393	2	0.8216
MN 108	0.06	2	0.9705
Soybean ‘Sturdy’	2.58	2	0.2752

[i] Df = degrees of freedom.

Table 7

Shoot biomass as determined by inoculation level of pennycress and soybean lines.

Crop	Biomass (g) at treatment level of
Crop	0 eggs/100 cm³	2,000 eggs/100 cm³	20,000 eggs/100 cm³
MN103	7.13	8.75	8.49
MN106	8.16	8.70	8.30
MN108	9.04	9.05	9.08
Susceptible soybean ‘Sturdy’	12.41	13.92	12.59

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DOI: https://doi.org/10.2478/jofnem-2022-0006 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X

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Language: English

Submitted on: Feb 16, 2021

Published on: Apr 11, 2022

Published by: Society of Nematologists, Inc.

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

alternative host,

Heterodera glycines,

host–parasitic relationship,

host suitability,

pennycress,

Thalapsi Arvense L.,

soybean cyst nematode

Related subjects:

Life sciences,

Life sciences, other

© 2022 Cody Hoerning, Senyu Chen, Katherine Frels, Donald Wyse, Samantha Wells, James Anderson, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 54 (2022): Issue 1 (February 2022)

Soybean Cyst Nematode Population Development and Its Effect on Pennycress in a Greenhouse Study

Figures & Tables

Figure 1

Table 1