Toxicity of Insecticides with Different Modes of Action to Apis Mellifera Larvae (Hymenoptera, Apidae)

Serial No.	Insecticide	Doses (Adult) Oral toxicity	Source
1	Fipronil (96.7% Purity)	0.005 μg, 0.01 μg 0.18 μg, 0.22 μg 0.30 μg, 0.38 μg	Sigma-Aldrich
2	Lambda-cyhalothrin (96.7% Purity)	0.28 μg, 0.55 μg 0.85 μg, 1.10 μg 1.50 μg, 1.70 μg	Sigma-Aldrich

Table 2.

Comparison between the larval observed mortality (OM %) and corrected mortality (CM %) for each tested concentration (Conc μg/larva) of fipronil using a probit analysis

Dose × 1000 (μg/larva)	Log (Dose) (X)	No of larvae treated (n)	No of dead larvae	Observed mortality (%)	Corrected mortality (%)	Empirical Probit (Y)	Expected probit
0.005	0.699	60	12	20.00	18.64	−0.891	−0.958
0.01	1.000	60	22	36.66	35.59	−0.369	−0.395
0.18	1.255	60	29	48.33	47.45	−0.064	0.082
0.22	1.342	60	36	60.00	59.32	0.236	0.245
0.30	1.477	60	40	66.66	66.09	0.415	0.497
0.38	1.580	60	49	81.66	81.35	0.891	0.689
0.00	0.00	60	1	1.66	-	-	-

[i] Regression equation Y = 1.8692× + 2.7337

[ii] Heterogeneity test

[iii] χ² cal = 0.673

[iv] χ² tab = 12.592

[v] LD₅₀(μg) = 0.163

[vi] Fiducial limit (μg) = 0.113 and 0.234

Table 3.

Comparison between the larval observed mortality (OM %) and corrected mortality (CM %) for each tested concentration (Conc μg/larva) of lambda-cyhalothrin using a probit analysis

Dose × 1000 (μg/larva)	Log (Dose) (X)	No of larva treated (n)	No of larva bees	Observed mortality (%)	Corrected mortality (%)	Empirical Probit (Y)	Expected probit
0.28	1.447	60	14	23.33	11.53	−1.198	−1.521
0.55	1.740	60	18	30.00	19.23	−0.869	−0.581
0.85	1.929	60	35	58.33	51.92	0.048	0.024
1.10	2.041	60	40	66.66	61.53	0.293	0.383
1.50	2.176	60	46	76.66	73.07	0.615	0.815
1.70	2.230	60	57	95.00	94.23	1.574	0.989
0.00	0.00	60	8	13.33	-	-	-

[i] Regression equation Y = 3.2087× - 1.1693

[ii] Heterogeneity test

[iii] χ² cal = 0.022

[iv] χ² tab = 9.488

[v] LD₅₀(μg) = 0.83

[vi] Fiducial limit (μg) = 0.667 and 1.047

References

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Articles in this issue

DOI: https://doi.org/10.2478/jas-2023-0010 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439 (formerly 2299-4831)

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Language: English

Page range: 115 - 123

Submitted on: Jul 7, 2023

Accepted on: Oct 30, 2023

Published on: Dec 22, 2023

Published by: The National Institute of Horticultural Research and Apicultural Research Association

In partnership with: Paradigm Publishing Services

Keywords:

Fipronil,

honey bee larvae,

Lambda-cyhalothrin,

lethal-dose mortality

Related subjects:

Life sciences,

Life sciences, other

© 2023 Mohammad Abdul Waseem, Meena Thakur, published by The National Institute of Horticultural Research and Apicultural Research Association
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 67 (2023): Issue 2 (December 2023)