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Determination of Quality Characteristics, Phenolic Compounds and Antioxidant Activity of Propolis from Southeastern Mexico Cover

Determination of Quality Characteristics, Phenolic Compounds and Antioxidant Activity of Propolis from Southeastern Mexico

Open Access
|Jun 2021

Figures & Tables

Fig. 1

Map of the location of apiaries in the southeast region of Mexico.RP= raw propolis. RP1: Maxcanú (N 20° 34′ 52.932″, W 89° 59′ 15.827″), RP2: Santa Cruz (N 20° 35′ 6.324″, W 89° 57′ 39.563″), RP3: Hecelchakán (N 20° 10′ 44.364″, W 90° 7′ 28.055″), RP4: Nunkiní (N 20° 23′ 28.356″, W 90° 8′ 58.38″), RP5: Halachó (N 20° 28′ 16.464″, W 90° 4′ 55.92″), RP6: Maxcanú (N 20° 35′ 11.724″, W 90° 0′ 27.792″), RP7: Pomuch (N 20° 08′ 16.00″, W 90° 10′ 28.0″), RP8: Calkiní (N 20° 22′ 10.524″, W 90° 3′ 6.804″), RP9: Cuch Holoch (N 20° 26′ 7.98″, W 90° 5′ 53.052″).
Map of the location of apiaries in the southeast region of Mexico.RP= raw propolis. RP1: Maxcanú (N 20° 34′ 52.932″, W 89° 59′ 15.827″), RP2: Santa Cruz (N 20° 35′ 6.324″, W 89° 57′ 39.563″), RP3: Hecelchakán (N 20° 10′ 44.364″, W 90° 7′ 28.055″), RP4: Nunkiní (N 20° 23′ 28.356″, W 90° 8′ 58.38″), RP5: Halachó (N 20° 28′ 16.464″, W 90° 4′ 55.92″), RP6: Maxcanú (N 20° 35′ 11.724″, W 90° 0′ 27.792″), RP7: Pomuch (N 20° 08′ 16.00″, W 90° 10′ 28.0″), RP8: Calkiní (N 20° 22′ 10.524″, W 90° 3′ 6.804″), RP9: Cuch Holoch (N 20° 26′ 7.98″, W 90° 5′ 53.052″).

Fig. 2

A typical chromatogram of the phenolic compounds detected in extracts PE1 (A), PE2 (B) and PE7 (C).
A typical chromatogram of the phenolic compounds detected in extracts PE1 (A), PE2 (B) and PE7 (C).

Fig. 3

PC1 vs. PC2 scatter plot; A) distinction between the samples (scores); (B) based on chemical quality (loadings).
PC1 vs. PC2 scatter plot; A) distinction between the samples (scores); (B) based on chemical quality (loadings).

Chemical quality of propolis extracts

Propolis extractDry extract (%)Solubility in PbSolubility in NaOHOxidation index (s)TPC (mg GAE/g)TF (mg QE/g)DPPH mM Trolox/gABTS mM Trolox/g
PE17.85±0.00d++16.16±0.55c33.66±0.48e11.93±0.00e0.61±0.01b2.29±0.13c
PE211.52±0.04h++6.00±0.00a97.02±5.40g42.68±1.79g2.71±0.01d4.64±0.30g
PE39.62±0.00f++12.03±0.55b28.28±1.55de1.79±0.00a1.60±0.03b2.50±0.00cd
PE46.02±0.03c++30.66±0.45e51.90±1.78f3.35±0.10ab0.66±0.04b2.88±0.04ef
PE511.24±0.14g++43.06±0.75f4.17±0.00a2.62±0.04ab0.20±0.01a1.35±0.10b
PE66.05±0.18c++17.56±0.89cd14.43±0.00b5.56±0.12c0.65±0.00b2.50±0.02cd
PE78.67±0.01e++42.56±0.75f19.32±0.52bc3.88±0.05bc0.26±0.00a0.92±0.00a
PE85.19±0.00b++13.36±0.63b23.31±0.04cd10.15±0.09d0.25±0.00a3.10±0.08f
PE92.30±0.00a++18.46±0.65d27.42±0.08d23.03±0.41f1.55±0.08c2.68±0.10de

Content of individual phenolic compounds (mg/100 g of dry propolis extract)

Samples123456789
PE1122.32±4.56229.13±6.79235.87±4.8772.99±0.8272.26±3.3962.40±0.8218.56±0.9823.09±2.0355.55±1.50
PE241.63±0.30106.86±1.28215.24±1.3162.48±1.3753.90±0.20172.36±5.623.10±0.19153.75±0.00388.26±25.46
PE322.17±0.46145.51±2.31207.09±4.6638.53±1.0755.19±0.4712.57±0.623.23±0.0528.72±0.0022.05±0.41
PE451.07±2.13140.60±0.98nd4.04±0.1274.35±3.42ndnd5.62±0.0013.61±0.33
PE510.30±0.03nd47.28±1.0610.30±0.4554.28±0.477.23±0.19nd2.35±0.0020.98±0.17
PE6215.01±8.6448.62±1.55 0.88±0.1279.45±0.548.86±0.112.26±0.07nd2.15±0.05
PE724.64±0.40nd273.28±1.739.42±0.2980.60±3.1162.21±0.00nd44.74±0.0053.02±2.29
PE831.12±0.68nd61.89±3.2416.59±0.0740.91±0.2126.05±0.854.19±0.0738.03±0.0060.36±1.92
PE9nd91.36±0.37nd50.51±2.2176.80±1.4219.66±0.1916.97±0.5622.46±0.0029.95±1.03

Moisture content, ash and sensory characteristics of raw propolis

SamplesMoisture (%)Ash (%)AppearanceAromaColorTasteConsistency
RP16.63±0.11e3.73±0.11eBright irregular piecesResinous softDark greenish brownInsipidMalleable
RP26.30±0.00e4.06±0.11fLow brightness irregular piecesResinousDark greenish brownInsipidRigid
RP34.63±0.11c2.26±0.11cBright irregular piecesOdorlessDark greenish brownInsipidMalleable
RP43.90±0.08b4.03±0.05fOpaque irregular piecesResinousReddish yellowPiquantRigid
RP56.66±0.05e0.66±0.05aOpaque irregular piecesOdorlessReddish yellowBitterMalleable
RP61.96±0.05a2.86±0.05dOpaque irregular piecesResinousBrownInsipidMalleable
RP76.53±0.30e1.83±0.05bOpaque irregular piecesResinous aromaticDark greenish brownPiquantMalleable
RP88.26±0.28f5.50±0.17gPowder or GranulesResinous softBrownInsipidRigid
RP95.50±0.17d2.03±0.05bcOpaque irregular piecesOdorlessDark greenish brownBitterMalleable

Pearson’s correlation between different parameters

ParametersABTSDPPHTFTPC
DPPH0.78*-
TF0.760.94-
TPC0.830.840.78-
Oxidation index−0.81−0.57−0.57−0.52
DOI: https://doi.org/10.2478/jas-2021-0008 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439
Language: English
Page range: 109 - 122
Submitted on: May 12, 2020
Accepted on: Dec 30, 2020
Published on: Jun 24, 2021
Published by: Research Institute of Horticulture
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2021 Enrique Sauri-Duch, Cesia Gutiérrez-Canul, Luis F. Cuevas-Glory, Lorena Ramón-Canul, Emilio Pérez-Pacheco, Víctor M. Moo-Huchin, published by Research Institute of Horticulture
This work is licensed under the Creative Commons Attribution 4.0 License.