Fig. 1
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Fig. 4
Characteristics, antioxidant (EC50 value) and anti-tyrosinase (IC50 value) activities of the crude (CME) and partitioned (MTMBP, DCMMBP and HXMBP) bee pollen extracts
| Sample | Weight (g) | Yield (%) | Appearance | EC50 (μg/mL) | IC50 (μg/mL) |
|---|---|---|---|---|---|
| Before partition | |||||
| CME | 85.76 | 61.3 | Turbid dark brown oil | - | - |
| After partition | |||||
| MTMBP | 51.87 | 60.5 | Dark brown oil | >1,000 | >500 |
| DCMMBP | 7.24 | 8.44 | Sticky dark brown solid | 192.1±17.83 | >500 |
| HXMBP | 8.14 | 9.49 | Dark brown oil | >1,000 | >500 |
| Reference standards | |||||
| Ascorbic acid | - | - | - | 89.8±5.07 | - |
| Kojic acid | - | - | - | - | 8.58±0.31 |
Characteristics, antioxidant (EC50 value) and anti-tyrosinase (% inhibition) activities of the different pooled fractions of DCMMBP after SiG60-CC and Sephadex LH-20 size exclusion CC
| Fraction | Weight (g) | Yield (%) | Appearance | EC50 (μg/mL) | Tyrosinase inhibition (%) |
|---|---|---|---|---|---|
| After SiG60-CC | |||||
| DCMMBP1 | 0.42 | 5.8 | Sticky dark brown solid | 819.5±24.37 | 2.86±1.75 |
| DCMMBP2 | 0.69 | 9.5 | Sticky dark brown solid | 284.1±11.92 | 2.99±3.18 |
| DCMMBP3 | 1.92 | 26.5 | Pale brown solid | 112.2±1.35 | 0 |
| Ascorbic acid | - | - | - | 81.1±1.44 | - |
| Kojic acid | - | - | - | - | 74.2±1.2 |
| After Sephadex LH-20 size exclusion CC | |||||
| DCMMBP3-1 | 1.6599 | 86.5 | Pale brown solid | 121.3±8.11 | - |
| DCMMBP3-2 | 0.0176 | 0.92 | Yellow solid | >1000 | - |
| Ascorbic acid | - | - | - | 81.1±1.44 | - |