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Evaluating Efficacy of Fumagilin-B® Against Nosemosis and Tracking Seasonal Trends of Nosema spp. in Nova Scotia Honey Bee Colonies Cover

Evaluating Efficacy of Fumagilin-B® Against Nosemosis and Tracking Seasonal Trends of Nosema spp. in Nova Scotia Honey Bee Colonies

Journal of Apicultural Science
Volume 64 (2020): Issue 2 (December 2020)
By: Robyn McCallum,  Sawyer Olmstead,  Jillian Shaw and  Kathleen Glasgow  
Open Access
|Nov 2020

Figures & Tables

Fig. 1

Mean Nosema ceranae spore loads (number of spores per bee x 106) ± SE in honey bee colonies in Nova Scotia (n = 72) from February 2018 to March 2019 after spring and autumn applications of Fumagilin-B® following label rate. The first arrow labelled ‘1’ indicates the spring application of Fumagilin-B® to treatment colonies, while the second arrow labelled ‘2’ indicates the autumn application of Fumagilin-B® to treatment colonies. Note: x axis for sampling date is not to scale.
Mean Nosema ceranae spore loads (number of spores per bee x 106) ± SE in honey bee colonies in Nova Scotia (n = 72) from February 2018 to March 2019 after spring and autumn applications of Fumagilin-B® following label rate. The first arrow labelled ‘1’ indicates the spring application of Fumagilin-B® to treatment colonies, while the second arrow labelled ‘2’ indicates the autumn application of Fumagilin-B® to treatment colonies. Note: x axis for sampling date is not to scale.

Honey bee sampling schedule and description from February 2018 to March 2019 in Nova Scotia colonies

DateDescription
28 February 2018First sample collection
27 March 2018Regular monthly sample collection
23 April 2018Pre spring treatment
24 & 25 April 2018Spring treatment and feeding (no samples collected)
27 April 20182–3 days post spring treatment
01 May 20185–6 days post spring treatment
07 May 201812–13 days post spring treatment
10 May 201815–16 days post spring treatment
29 May 201834–35 days post spring treatment/Regular monthly sample collection
26 June 2018Regular monthly sample collection
26 July 2018Regular monthly sample collection
29 August 2018Regular monthly sample collection
03 October 2018Pre fall treatment
05 & 06 October 2018Fall treatment and feeding (no samples collected)
10 October 20184–5 days post fall treatment
16 October 201810–11 days post fall treatment
19 October 201813–14 days post fall treatment
29 October 201823–24 days post fall treatment
14 March 2019Final sample collection

List of primers used to identify Nosema species

TargetPrimerSequence (5′–3′)Amplicon size (bp)
RPS5RPS5-FAATTATTTGGTCGCTGGAATTG105
RPS5-RTAACGTCCAGCAGAATGTGGTA
RNA polymeraseNcpol-FTGG GTT CCC TAA ACC TGG TGG TTT662
Ncpol-RTCA CAT GAC CTG GTG CTC CTT CT297
Napol-FAGC AAG AGA CGT TTC TGG TAC CTC A
Napol-RCCT TCA CGA CCA CCC ATG GCA
DOI: https://doi.org/10.2478/jas-2020-0025 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439
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Language: English
Page range: 277 - 286
Submitted on: Jan 28, 2020
Accepted on: Oct 3, 2020
Published on: Nov 18, 2020
Published by: Research Institute of Horticulture
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
Fumagilin-B,
honey bees,
spp
Related subjects:
Life sciences,
Life sciences, other,
Zoology

© 2020 Robyn McCallum, Sawyer Olmstead, Jillian Shaw, Kathleen Glasgow, published by Research Institute of Horticulture
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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