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Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers Cover

Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers

Journal of Apicultural Science
Volume 64 (2020): Issue 2 (December 2020)
By: Anais Rodríguez,  Carlos A. Yadró,  Adolfo Pérez,  Ciro Invernizzi and  Ivanna Tomasco  
Open Access
|Oct 2020

Figures & Tables

Fig. 1

Map of sampled locations. PR: Pinar del Rio, LH: La Habana, MY: Mayabeque, MT: Matanzas, LT: Las Tunas, HO: Holguín, SC: Santiago de Cuba, GT: Guantánamo. Black triangles represent localization on the sampled apiaries.
Map of sampled locations. PR: Pinar del Rio, LH: La Habana, MY: Mayabeque, MT: Matanzas, LT: Las Tunas, HO: Holguín, SC: Santiago de Cuba, GT: Guantánamo. Black triangles represent localization on the sampled apiaries.

Fig. 2

Restriction profiles of COI region of Varroa destructor mtDNA. Eight provinces of Cuba were sampled and displayed numbered from 1 to 8. 1: PR, 2: LH, 3: MY, 4: MT, 5: LT, 6: HO, 7: SC and 8:GT. The mtDNA was digested with endonucleases SacI and EcoNI. Lane L: 100-bp DNA ladder, Lane A: Undigested amplicons (amplified with COXF and COXRa primers) and Lane B: Undigested amplicons (amplified with VD-CO206F and VD-CO206R).
Restriction profiles of COI region of Varroa destructor mtDNA. Eight provinces of Cuba were sampled and displayed numbered from 1 to 8. 1: PR, 2: LH, 3: MY, 4: MT, 5: LT, 6: HO, 7: SC and 8:GT. The mtDNA was digested with endonucleases SacI and EcoNI. Lane L: 100-bp DNA ladder, Lane A: Undigested amplicons (amplified with COXF and COXRa primers) and Lane B: Undigested amplicons (amplified with VD-CO206F and VD-CO206R).

Microsatellites absolute sizes and frequencies of alleles found in Cuba_ He and Ho are expected and observed heterocigocity, respectively

Allele size (bp)Localities
Locussequenced *this studyMayabequeLa Habana
N=12N=12
VD1121421580.040.04
1600.000.08
1680.880.58
1780.080.29
Ho0.250.67
He0.230.57
N=11N=12
VD1142172460.090.08
2500.910.92
Ho0.000.00
He0.170.15
N=12N=12
VD01626831611
Ho00
He00
DOI: https://doi.org/10.2478/jas-2020-0022 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439
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Language: English
Page range: 335 - 343
Submitted on: May 27, 2020
Accepted on: Jul 13, 2020
Published on: Oct 24, 2020
Published by: Research Institute of Horticulture
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
DNA mitochondrial,
haplotype,
microsatellites,
STR
Related subjects:
Life sciences,
Zoology,
Life sciences, other

© 2020 Anais Rodríguez, Carlos A. Yadró, Adolfo Pérez, Ciro Invernizzi, Ivanna Tomasco, published by Research Institute of Horticulture
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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