Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers

Rodríguez, Anais; Yadró, Carlos A.; Pérez, Adolfo; Invernizzi, Ciro; Tomasco, Ivanna

doi:10.2478/jas-2020-0022

Abstract

Varroa destructor has been present in Cuba since 1996, but without the use of acaricidal infestation rates remain at very low levels. The presence of Korean haplotype mites was described in 2007, but there is no information regarding the introgression of the less virulent Japanese haplotype that could account for a low pathogenicity of the mite. In this research, we carried out molecular characterization of Cuban Varroa mites through mitochondrial DNA and hypervariable nuclear loci. We applied an alternative RFLP technique and found that all the analyzed samples corresponded to Korean haplotypes. We analyzed the three STRs loci VD112, VD114 and VD016, previously described as highly variable and found new alleles in all of them, with an absolute allele size very different to those reported worldwide. We also detected genic and genotypic differentiation between samples from two nearby locations (P=0.08). We also tested a new RFLP method for mite haplotype discrimination with an intra-reaction positive control of digestion.

References

Anderson, D. L., & Fuchs, S. (1998). Two genetically distinct populations of Varroa jacobsoni with contrasting reproductive abilities on Apis mellifera. Journal of Apicultural Research, 37(2), 69–78. https://doi.org/10.1080/00218839.1998.11100957
Search in Google Scholar Back to article
Anderson, D. L., & Trueman, J. W. H. (2000). Varroa jacobsoni (Acari: Varroidae) is more than one species. Experimental & Applied Acarology, 24, 165–189. https://doi.org/10.1023/A:1006456720416
Search in Google Scholar Back to article
Beaurepaire, A. L., Moro, A., Mondet, F., Le Conte, Y., Neumann, P., Locke, B. (2019). Population genetics of estoparasitic mites suggest arms race with honeybee hosts. Scientific Reports, 9, 11355. https://doi:10.1038/s41598-019-47801-5
Search in Google Scholar Back to article
Boecking, O., & Genersch, E. (2008). Varroosis–the ongoing crisis in bee keeping. Journal für Verbraucherschutz und Lebensmittelsicherheit, 3, 221–228. https://doi.org/10.1007/s00003-008-0331-y
Search in Google Scholar Back to article
de Guzman, L. I., & Rinderer, T. E. (1999). Identification and comparison of Varroa species infesting honey bees. Apidologie, 30(2–3), 85–95. https://doi.org/10.1051/apido:19990201
Search in Google Scholar Back to article
de Mattos, I. M., De Jong, D., & Soares, A. E. E. (2016). Island population of European honey bees in Northeastern Brazil that have survived Varroa infestations for over 30 years. Apidologie, 47, 818–827. https://doi.org/10.1007/s13592-016-0439-5
Search in Google Scholar Back to article
Demedio, J. (2001). La varroasis de las abejas en una zona de la provincia de La Habana. Agente etiológico, índices de infestación y control biotécnico y químico. Tesis en opción al grado Doctor en Ciencias Veterinarias.
Search in Google Scholar Back to article
Domínguez, S. (2004). Alternativa de lucha contra el parásito Varroa ss/pp. 1er Congreso de Apicultura.
Search in Google Scholar Back to article
Eliash, N., & Mikheyev, A. (2020). Varroa mite evolution: a neglected aspect of worldwide bee collapses? Current Opinion in Insect Science, 39, 21–26. https://doi.org/10.1016/j.cois.2019.11.004
Search in Google Scholar Back to article
Fries, I., Hansen, H., Imdorf, A., & Rosenkranz, P. (2003). Swarming in honey bees (Apis mellifera) and Varroa destructor population development in Sweden. Apidologie, 34(4), 389–397. https://doi.org/10.1051/apido:2003032
Search in Google Scholar Back to article
García, A. (2004). Uso de las hojas de Jubabán (Trichilia hirtal) como potenciadoras en la acción contra la Varroa ss/pp. 1er Congreso de Apicultura.
Search in Google Scholar Back to article
Guerra Jr, J. C. V., Issa, M. R. C., Carneiro, F. E., Strapazzon, R., Moretto, G. (2010). RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas. Genetics and Molecular Research, 9(1), 303–308. https://doi.org/10.4238/vol9-1gmr696
Search in Google Scholar Back to article
Martin, S. J., Highfield, A. C., Brettell, L., Villalobos, E. l. M., Budge, G. E., Powell, M., Schroeder, D. C. (2012). Global honey bee viral landscape altered by a parasitic mite. Science, 336(6086), 1304–1306. https://doi.org/10.1126/science.1220941
Search in Google Scholar Back to article
Miller, S. A., Dykes, D. D., & Polesky, H. F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research, 16(3), 1215. https://doi.org/10.1093/nar/16.3.1215
Search in Google Scholar Back to article
Muñoz, I., Garrido-Bailón, E., Martín-Hernández, R., Meana, A., Higes, M., De la Rúa, P. (2008). Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies. Journal of Apicultural Research, 47(4), 310–313. https://doi.org/10.3896/IBRA.1.47.4.13
Search in Google Scholar Back to article
Navajas, M., Le Conte, Y., Solignac, M., Cros-Arteil, S., Cornuet, J-M. (2002). The complete sequence of the mitochondrial genome of the honeybee ectoparasite mite Varroa destructor (Acari: Mesostigmata). Molecular Biology and Evolution, 19(12), 2313–2317. https://doi.org/10.1093/oxfordjournals.molbev.a004055
Search in Google Scholar Back to article
Navajas M., Anderson D. L., De Guzman L. I., Huang Z. Y., Clement J., Zhou T., Le Conte Y. (2010). New Asian types of Varroa destructor: a potential new threat for world apiculture. Apidologie, 41, 181–193. https://doi.org/10.1051/apido/2009068
Search in Google Scholar Back to article
Peakall, R. O. D., & Smouse, P. E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
Search in Google Scholar Back to article
Pérez, A. (2014). Los hábitos higiénicos de Apis mellifera (Hymenoptera: Apidae) en Cuba en 2013 y su relación con la tasa de infestación de Varroa destructor (Mesostigmata: Varroidae) y la producción de miel. (Bachiller en Ciencias), Universidad de La Habana.
Search in Google Scholar Back to article
Ramsey, S. D., Ochoa, R., Bauchan, G., Gulbronson, C., Mowery, J. D., Cohen, A., Ellis, J. D. (2019). Varroa destructor feeds primarily on honey bee fat body tissue and not hemolymph. Proceedings of the National Academy of Sciences, 116(5), 1792–1801. https://doi.org/10.1073/pnas.1818371116
Search in Google Scholar Back to article
Raymond, M., & Rousset, F. (1995). GENEPOP Version 1.2: population genetics software for exact tests and ecumenicism. Journal of Heredity, 86(3), 248–249. https://doi.org/10.1093/oxfordjournals.jhered.a111573
Search in Google Scholar Back to article
Rosenkranz, P., Aumeier, P., & Ziegelmann, B. (2010). Biology and control of Varroa destructor. Journal of Invertebrate Pathology, 103, S96–S119. https://doi.org/10.1016/j.jip.2009.07.016
Search in Google Scholar Back to article
Traynor, K., Mondet, F., de Miranda, J., Techer, M., Kowallik, V., Oddie, M., … McAfee, A. (2020). Varroa destructor: A Complex Parasite, Crippling Honeybees Worldwide. Preprints, 2020020374. https://doi.org/10.20944/preprints202002.0374.v1
Search in Google Scholar Back to article
Sanabria, J. L. (2007). Índices de infestación, estatus racial y expresión de mecanismos de resistencia en colmenas sin control antivarroa. Tesis en opción al grado científico de Doctor en Ciencias Veterinarias. Universidad Agraria de La Habana (UNAH).
Search in Google Scholar Back to article
Sanguinetti, C. J., Dias, E. N., & Simpson, A. J. (1994). Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques, 17, 914–921.
Search in Google Scholar Back to article
Smith, J. M., & Haigh, J. (1974). The hitch-hiking effect of a favourable gene. Genetics Research, 23, 23–35. https://doi.org/10.1017/S0016672300014634. PMID 4407212.
Search in Google Scholar Back to article
Solignac, M., Cornuet, J-M., Vautrin, D., Le Conte, Y., Anderson, D., Evans, J. D., . . . Navajas, M. (2005). The invasive Korea and Japan types of Varroa destructor, ectoparasitic mites of the Western honeybee (Apis mellifera), are two partly isolated clones. Prodeedings of the Royal Society B: Biologial Sciences, 272, 411–419. https://doi.org/10.1098/rspb.2004.2853
Search in Google Scholar Back to article
Solignac, M., Vautrin, D., Pizzo, A., Navajas, M., Le Conte, Y., Cornuet, J-M. (2003). Characterization of microsatellite markers for the apicultural pest Varroa destructor (Acari: Varroidae) and its relatives. Molecular Ecology Notes, 3(4), 556–559. https://doi.org/10.1046/j.1471-8286.2003.00510.x
Search in Google Scholar Back to article
Strapazzon, R., Carneiro, F. E., Guerra-Júnior, J., & Moretto, G. (2009). Genetic characterization of the mite Varroa destructor (Acari: Varroidae) collected from honey bees Apis mellifera (Hymenoptera: Apidae) in the state of Santa Catarina, Brazil. Genetics and Molecular Research, 8(3), 990–997. https://doi.org/10.4238/vol8-3gmr567
Search in Google Scholar Back to article
Utzeri, V. J., Schiavo, G., Ribani, A., Bertolini, F., Bovo, S., Fontanesi, L. (2019). A next generation sequencing approach for targeted Varroa destructor (Acari: Varroidae) mitochondrial DNA analysis based on honey derived environmental DNA. Journal of Invertebrate Pathology, 161, 47–53. https://doi.org/10.1016/j.jip.2019.01.005
Search in Google Scholar Back to article
Van Oosterhout, C., Hutchinson, W. F., Wills, D. P. M., & Shipley, P. (2004). MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 4, 535–538. https://doi.org/10.1111/j.1471-8286.2004.00684.x
Search in Google Scholar Back to article
Verde, M., & Demedio, J. (1998). Algunas consideraciones sobre el costo-beneficio del tratamiento con Bayvarol empleado para el control de la varroasis (Varroa jacobsoni Oud.) en Cuba. Universidad Agraria de La Habana.
Search in Google Scholar Back to article
Yue, C., & Genersch, E. (2005). RT-PCR analysis of Deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor). Journal of General Virology, 86(12), 3419–3424. https://doi.org/10.1099/vir.0.81401-0
Search in Google Scholar Back to article

Characterization of Varroa destructor Mites in Cuba Using Mitochondrial and Nuclear Markers

Abstract

Paradigm

My account