Influence of the Botanical Origin of Honey from North Western Spain in Some Antioxidant Components

Escuredo, Olga; Fernández-González, María; Rodríguez-Flores, María S.; Seijo-Rodríguez, Ana; Seijo-Coello, María C.

doi:10.2478/jas-2013-0001

Abstract

Honeys were studied in order to know the relationships between their botanical origin, the total content of phenols and flavonoids, and the 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The principal floral resources for honeybees in the studied area were Castanea sativa, Rubus, Eucalyptus, Erica, and Cytisus.

Results showed the influence of Erica and Castanea in the phenol content, flavonoids content, and the antioxidant activity of honeys. The presence of Erica in honeys was related to the highest phenol content; so heather honeys had a mean content of 178.9 mg/100g. Honeys with high Castanea pollen content had the highest value of flavonoids (7.7 mg/100g) and a minor value of half maximal inhibitory concentration (IC₅₀) (9.4 mg/ml). Eucalyptus and blackberry were the honeys with minor contents of these compounds. A stepwise linear regression was used to estimate the phenol content of honeys. The contribution of Erica and Castanea was highlighted in the obtained model (R² = 0.61), with significant p-value (< 0.01).

References

Al M. L, Daniel D., Moise A., Bobis O., Laslo L., Bogdanov S. (2009) - Physicochemical and bioactive properties of different floral origin honeys from Romania. FoodChem., 112: 863-867.
Search in Google Scholar
Arvouet-Grand A., Vennat B., Pourrat A., Legret P. (1994) - Standardisation d’un extrait de propolis et identification des principaux constituants. J. Pharm. Belg., 49: 462-468.
Search in Google Scholar
Ay M., Bahadori F., Öztürk M., Kolak U., Topçu G. (2007) - Antioxidant activity of Erica arborea. Fitoterapia, 78: 571-573.10.1016/j.fitote.2007.03.02417570611
Search in Google Scholar
Ball D. W. (2007) - The chemical composition of honey. J. Chem. Educ., 84: 1643-1646.
Search in Google Scholar
Bedascarrasbure E., Maldonado L., Morales W. F., Álvarez A. (2006) - Propóleos. Magna Ed., pp 218.
Search in Google Scholar
Beretta G., Granata P., Ferrero M., Orioli M., Facino R. M. (2005) - Standardization of antioxidant properties of honey by a combination of spectrophotometric/ fluorimetric assays and chemometrics. Anal. Chim. Acta, 533: 185-191.
Search in Google Scholar
Bertoncelj J., Doberšek U., Jamnik M., Golob T. (2007) - Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem., 105: 822-828.
Search in Google Scholar
Brand-Williams W., Cuvelier M. E., Berset C. (1995) - Use of a free radical method to evaluate antioxidant activity. LWTFoodSci. Technol., 28: 25-30.
Search in Google Scholar
Chen L., Mehta A., Berenbaum M., Zangerl A. R., Engeseth N. J. (2000) - Honeys from Different Floral Sources as Inhibitors of Enzymatic Browning in Fruit and Vegetable Homogenates. J. Agric. FoodChem., 48: 4997-5000.
Search in Google Scholar
Estevinho L., Pereira A. P., Moreira A. P., Dias L. G., Pereira E. (2008) - Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem. Toxicol., 46: 3774-3779.
Search in Google Scholar
Ferreira I. C. F. R., Aires E., Barreira J. C. M., Estevinho L. M. (2009) - Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem., 114: 1438-1443.10.1016/j.foodchem.2008.11.028
Search in Google Scholar
Frankel S., Robinson G. E., Berenbaum M. R. (1998) - Antioxidant capacity and correlation characteristics of 14 unifloral honeys. J. Apic. Res., 37: 27-31.
Search in Google Scholar
Karadag A., Ozcelik B., Saner S. (2009) - Review of methods to determine antioxidant capacities. Food Anal. Methods, 2: 41-60.
Search in Google Scholar
Küçük M., Kolayl S., Karaoglu S., Ulusoy E., Baltac C., Candan F. (2007) - Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chem., 100: 526-534.
Search in Google Scholar
Lachman J., Orsák M., Hejtmánková A., Kovárová E. (2010) - Evaluation of antioxidant activity and total phenolics of selected Czech honeys. Food Sci. Technol., 43: 52-58.
Search in Google Scholar
Louveaux J., Maurizio A., Vorwohl G. (1978) - Methods of melissopalynology. BeeWorld, 59: 139-157. 10.1080/0005772X.1978.11097714
Search in Google Scholar
Luís Â., Domingues F., Gil C., Duarte A. P. (2009) - Antioxidant activity of extracts of Portuguese shrubs: Pterospartumtridentatum, Cytisus scoparius and Erica spp. J. Med. Plant. Res., 3: 886-893.
Search in Google Scholar
Meda A., Lamien C. E., Romito M., Millogo J., Nacoulma O. G. (2005) - Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem., 91: 571-577.10.1016/j.foodchem.2004.10.006
Search in Google Scholar
Nagai T., Inoue R., Kanamori N., Suzuki N., Nagashima T. (2006) - Characterization of honey from different floral sources. Its functional properties and effects of honey species on storage of meat. Food Chem., 97: 256-262.
Search in Google Scholar
Nazemiyeh H., Bahadori F., Delazar A., Ay M., Topcu G., Nahar L., Majinda R. R. T., Sarker S. D. (2008) - Antioxidant phenolic compounds from the leaves of Erica arborea (Ericaceae). Nat. Prod. Res., 22: 1385-1392.
Search in Google Scholar
Okada Y., Okada M. (1998) - Scavenging effect of water soluble proteins in broad beans on free radicals and active oxygen species. J. Agric. Food Chem., 46: 401-406.
Search in Google Scholar
Pavlović R. D., Lakušić B., Došlov- Kokoruš Z., Kovačević N. (2009) - Arbutin content and antioxidant activity of some Ericaceae species. Pharmazie, 64: 656-659.
Search in Google Scholar
Persano-Oddo L., Heard T. A., Rodríguez-Malaver A., Pérez R. A., Fernández-Muiño M., Sancho M. T., Sesta G., Lusco L., Vit P. (2008) - Composition and antioxidant activity of Trigona carbonaria honey from Australia. J. Med. Food, 11: 789-94.
Search in Google Scholar
Pichichero E., Canuti L., Canini A. (2009) - Characterisation of the phenolic and flavonoid fractions and antioxidant power of Italian honeys of different botanical origin. J. Sci. Food Agric. 89: 609-616.10.1002/jsfa.3484
Search in Google Scholar
Prior R. L., Wu X., Schaich K. (2005) - Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric. Food Chem., 53: 4290-4302.
Search in Google Scholar
Sánchez-Moreno C. (2002) - Review: methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci. Tech. Int., 8: 121-137.
Search in Google Scholar
Sánchez-Moreno C., Larrauri J. A., Saura-Calixto F. (1998) - A procedure to measure the antiradical efficiency of polyphenols. J. Sci. Food Agric., 76: 270-276.
Search in Google Scholar
Seijo M. C., Jato M. V. (1998) - Palynological characterization of honeys from Galicia (Northwest Spain). Grana, 37: 285-292.10.1080/00173139809362680
Search in Google Scholar
Seijo M. C., Escuredo O., Fernández- González M. (2011) - Fungal diversity in honeys from northwest Spain and their relationship to the ecological origin of the product. Grana, 50:55-62.10.1080/00173134.2011.559555
Search in Google Scholar
Singleton V. L., Rossi J. A. (1965) - Colorymetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 16: 144-158.
Search in Google Scholar
Soria A. C., González M., De Lorenzo C., Martínez-Castro I., Sanz J. (2005) - Estimation of the honeydew ratio in honey samples from their physicochemical data and from their volatile composition obtained by SPME and GC-MS. J. Sci. Food Agric., 85: 817-824.
Search in Google Scholar
Tabart J., Kevers C., Pincemail J., Defraigne J. O., Dommes J. (2009) - Comparative antioxidant capacities of phenolic compounds measured by various tests. FoodChem., 113: 1226-1233.
Search in Google Scholar
Vela L., De Lorenzo C., Pérez R. A. (2007) - Antioxidant capacity of Spanish honeys and its correlation with polyphenol content and other physicochemical properties. J. Sci. Food Agric., 87: 1069-1075.
Search in Google Scholar
Vit P., Gutiérrez M. G., Titera D., Bednar M., Rodríguez-Malaver A. J. (2008) - Mieles checas categorizadas según su actividad antioxidante. Acta Bioquim. Clin. Latinoam., 42: 237-44.
Search in Google Scholar
Von der Ohe W (Ed.) (2004) - European unifloral honeys. Apidologie, Special issue 35: 1-112.
Search in Google Scholar
Waś E., Rybak-Chmielewska H., Sczesna T., Kachaniuk K., Teper D. (2011). Characteristics of Polish unifloral honeys. III. Heather honey (Calluna vulgaris L.). J. Apic. Sci., 55: 129-135
Search in Google Scholar
Yao L. H., Jiang Y. M., Shi J., Tomás-Barberán F. A., Datta N., Singanusong R., Chen S. S. (2004) - Flavonoids in food and their health benefits. Plant Food Hum. Nutr., 59: 113-122.
Search in Google Scholar

Influence of the Botanical Origin of Honey from North Western Spain in Some Antioxidant Components

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