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Green synthesis of zinc oxide (ZnO) nanoparticles using aqueous leaves extracts of Artemisia annua: Their characterizations and anti-parasitic activity Cover

Green synthesis of zinc oxide (ZnO) nanoparticles using aqueous leaves extracts of Artemisia annua: Their characterizations and anti-parasitic activity

Open Access
|Dec 2025

References

  1. Abd El Hady, W.E., El-Emam, G.A., Saleh, N.E., Hamouda, M.M., Motawea, A. (2023): The idiosyncratic efficacy of spironolactone-loaded PLGA nanoparticles against murine intestinal schistosomiasis. Int J Nanomedicine, 18: 987 – 1005. DOI: 10.2147/IJN.S389449
  2. Abd El Wahab, W.M., El-Badry, A.A., Mahmoud, S.S., El-Badry, Y.A., El-Badry, M.A., Hamdy, D.A. (2021): Ginger (Zingiber officinale)-derived nanoparticles in Schistosoma mansoni infected mice: hepatoprotective and enhancer of etiological treatment. PLoS Negl Trop Dis, 15: e0009423. DOI: 10.1371/journal.pntd.0009423
  3. Abou El-Nour, M.F., Fadladdin, Y. (2021): Antischistosomal activity of Zingiber officinale, Piper nigrum, and Coriandrum sativum aqueous plant extracts on hamsters infected with Schistosoma mansoni. J Parasitol Res, 2021: 6628787. DOI: 10.1155/2021/6628787
  4. Abou El-Nour, M.F., Kenawy, S.H., El-Bassyouni, G.T., Hamzawy, E.M. (2021): A novel treatment of schistosomiasis: nano-calcium silicate incorporating 5 % copper oxide. Adv Pharm Bull, 11: 68 – 76. DOI: 10.34172/apb.2021.004
  5. Aires, A.L., Ximenes, E.C.P.A., Barbosa, V.X., Góes, A.J.S., Souza, V.M.O., Albuquerque, M.C.P.A. (2014): β-Lapachone: a naphthoquinone with promising antischistosomal properties in mice. Phytomedicine, 21: 261 – 267. DOI: 10.1016/j.phymed.2013.08.012
  6. Aldeen, T.S., Mohamed, H.E.A., Maaza, M. (2022): ZnO nanoparticles prepared via a green synthesis approach: physical properties, photocatalytic and antibacterial activity. J Phys Chem Solids, 160: 110313. DOI: 10.1016/j.jpcs.2021.110313
  7. Alkhtaby, L.A., Abou El-Nour, M., Eldera, S.S. (2025): Characterization of green synthesis silver nanoparticles extracts by Eucalyptus citratus aqueous leaves and evaluated against Schistosoma mansoni. J Biomed Mater Res B Appl Biomater, 113: e35629. DO: 10.1002/jbm.b.35629
  8. Al-Radadi, N.S., Abdullah, Faisal, S., Alotaibi, A., Ullah, R., Hussain, T., Rizwan, M., Saira, Z., Zaman, N., Iqbal, M., Iqbal, R., Ali, Z. (2022): Zingiber officinale driven bioproduction of ZnO nanoparticles and their anti-inflammatory, anti-diabetic, anti-Alzheimer, anti-oxidant, and anti-microbial applications. Inorg Chem Commun, 140: 109274. DOI: 10.1016/j.inoche.2022.109274
  9. Baggieri, M., Gioacchini, S., Borgonovo, G., Catinella, G., Marchi, A., Picone, P., Vasto, S., Fioravanti, R., Bucci, P., Kojouri, M., Giuseppetti, R., D'Ugo, E., Ubaldi, F., Dallavalle, S., Nuzzo, D., Pinto, A., Magurano, F. (2023): Antiviral, virucidal and antioxidant properties of Artemisia annua against SARS-CoV-2. Biomed Pharmacother, 168: 115682. DOI: 10.1016/j.biopha.2023.115682
  10. Chaudhary, P., de Araújo, V.C., Ramos, M.V., Kumar, V.L. (2015): Antiedematogenic and antioxidant properties of high molecular weight protein sub-fraction of Calotropis procera latex in rat. J Basic Clin Pharm, 6: 69 – 73. DOI: 10.4103/0976-0105.152098
  11. Chavan, C., Prabhune, S., Shedge, S., Patwardhan, R., Kamble, S., Murthy, A.V.R., Kale, S.N. (2021): Studies on drug-assisted silver nanoparticles to reduce granulocytopenia and improve drug delivery for cancer therapy. Appl Phys A, 127: 332. DOI: 10.1007/s00339-021-04468-y
  12. Cioli, D., Pica-Mattoccia, L., Basso, A., Guidi, A. (2014): Schistosomiasis control: praziquantel forever? Mol Biochem Parasitol, 195: 23 – 29. DOI: 10.1016/j.molbiopara.2014.06.002
  13. Danaei, M., Dehghankhold, M., Ataei, S., Davarani, F.H., Javanmard, R., Dokhani, A., Khorasani, S., Mozafari, M.R. (2018): Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics, 10: 57. DOI: 10.3390/pharmaceutics10020057
  14. Dkhil, M.A., Khalil, M.F., Diab, M.S.M., Bauomy, A.A., Santourlidis, S., Al-Shaebi, E.M., Al-Quraishy, S. (2019): Evaluation of nanoselenium and nanogold activities against murine intestinal schistosomiasis. Saudi J Biol Sci, 26: 1468 – 1472. DOI: 10.1016/j.sjbs.2018.02.008
  15. Eldera, S., Alkhtaby, L.A., Al-Wafi, R., Abou El-Nour, M.F. (2025): Biosynthesis of zinc oxide nanoparticles by Origanum majorana aqueous leaf extract: characterization and evaluation against Schistosoma haematobium. J Biomed Mater Res B Appl Biomater, 113: e35538. DOI: 10.1002/jbm.b.35538
  16. Eldera, S.S., Hamzawy, E.M.A., Aldawsari, S., Al-Wafi, R., Abou El-Nour, M.F. (2025): Eco-friendly green synthesis of silver nanoparticles with Syzygium aromaticum extract: characterization and evaluation against Schistosoma haematobium. Rev Adv Mater Sci, 64: 20250166. DOI: 10.1515/rams-2025-0166
  17. El-Derbawy, M.M., Salem, H.S., Raboo, M., Baiuomy, I.R., Fadil, S.A., Fadil, H.A., Ibrahim, S.R.M., El-Kholy, W.A. (2022): In vivo evaluation of the anti-schistosomal potential of ginger-loaded chitosan nanoparticles on Schistosoma mansoni: histopathological, ultrastructural and immunological changes. Life, 12: 1834. DOI: 10.3390/life12111834
  18. Fadladdin, Y.A.J. (2021): Antischistosomal activity of Origanum majorana, Ziziphus spina-christi and Salvia fruticosa plant extracts on hamsters infected with Schistosoma haematobium. BioMed Res Int, 2021: 5545331. DOI: 10.1155/2021/5545331
  19. Fadladdin, Y.A.J. (2022): Evaluation of antischistosomal activities of crude aqueous extracts of Artemisia annua, Nigella sativa and Allium sativum against Schistosoma mansoni in hamsters. BioMed Res Int, 2022: 5172287. DOI: 10.1155/2022/5172287
  20. Faisal, S., Abdullah, Rizwan, M., Ullah, R., Alotaibi, A., Khattak, A., Bibi, N., Idrees, M. (2022): Paraclostridium benzoelyticum-mediated zinc oxide nanoparticles and their in vivo multiple biological applications. Oxid Med Cell Longev, 2022: 5994033. DOI: 10.1155/2022/5994033
  21. Faisal, S., Al-Radadi, N.S., Jan, H., Abdullah, Shah, S.A., Shah, S., Rizwan, M., Afsheen, Z., Hussain, Z., Uddin, M.N., Idrees, M., Bibi, N. (2021): Curcuma longa-mediated synthesis of copper oxide, nickel oxide and Cu–Ni bimetallic hybrid nanoparticles: characterization and evaluation for antimicrobial, antiparasitic and cytotoxic potentials. Coatings, 11: 849. DOI: 10.3390/coatings11070849
  22. Faisal, S., Jan, H., Shah, S.A., Shah, S., Khan, A., Akbar, M.T., Rizwan, M., Jan, F., Wajidullah, Akhtar, N., Khattak, A., Syed, S. (2021): Green synthesis of zinc oxide nanoparticles using aqueous fruit extracts of Myristica fragrans: characterization and biological and environmental applications. ACS Omega, 6: 9709 – 9722. DOI: 10.1021/acsomega.1c00310
  23. Faisal, S., Khan, M.K., Jan, H., Shah, S.A., Abdullah, Shah, S., Rizwan, M., Wajidullah, Akbar, M.T., Redaina (2021): Edible mushroom (Flammulina velutipes) as a biosource for silver nanoparticles: from synthesis to diverse biomedical and environmental applications. Nanotechnology, 32: 065101. DOI: 10.1088/1361-6528/abc2eb
  24. Fernandes, C.A., Jesudoss, N., Nizam, A., Krishna, S.N.B., Lakshmaiah, V.V. (2023): Biogenic synthesis of zinc oxide nanoparticles mediated by Terminalia catappa fruit pericarp extract and its multifaceted applications. ACS Omega, 8: 39315 – 39328. DOI: 10.1021/acsomega.3c04857
  25. Fuzimoto, A.D. (2021): An overview of the anti-SARS-CoV-2 properties of Artemisia annua, its antiviral action, protein-associated mechanisms and repurposing for COVID-19 treatment. J Integr Med, 19: 375 – 388. DOI: 10.1016/j.joim.2021.07.003
  26. Gendrot, M., Duflot, I., Boxberger, M., Delandre, O., Jardot, P., Le Bideau, M., Andreani, J., Fonta, I., Mosnier, J., Rolland, C., Hutter, S., La Scola, B., Pradines, B. (2020): Antimalarial artemisinin-based combination therapies and COVID-19 in Africa: in vitro inhibition of SARS-CoV-2 replication by mefloquine-artesunate. Int J Infect Dis, 99: 437 – 440. DOI: 10.1016/j.ijid.2020.08.032
  27. Greenberg, R.M. (2005): Ca2+ signalling, voltage-gated Ca2+ channels and praziquantel in flatworm neuromusculature. Parasitology, 131(Suppl 1): S97 – S108. DOI: 10.1017/S0031182005008346
  28. Hamdan, Z.K., Soliman, M.I., Taha, H.A., Khalil, M.M.H., Nigm, A.H. (2023): Antischistosomal effects of green and chemically synthesized silver nanoparticles: in vitro and in vivo murine model. Acta Trop, 244: 106952. DOI: 10.1016/j.actatropica.2023.106952
  29. Hamidpour, R., Hamidpour, S., Hamidpour, M., Shahlari, M., Sohraby, M., Shahlari, N., Hamidpour, R. (2017): Russian olive (Elaeagnus angustifolia L.): from traditional medicinal applications to novel antioxidant, anti-inflammatory, antimutagenic and analgesic roles. J Tradit Complement Med, 7: 24 – 29. DOI: 10.1016/j.jtcme.2015.09.004
  30. Hazman, Ö., Khamidov, G., Yilmaz, M.A., Bozkurt, M.F., Kargioğlu, M., Savrik, M., Tukhtae, D., Erol, I. (2024): Green synthesis of silver nanoparticles from Verbascum insulare and Heldreichia spp.: antimicrobial, anticancer, antioxidant and photocatalytic activities. J Photochem Photobiol A Chem, 453: 115601. DOI: 10.1016/j.jphotochem.2024.115601
  31. Hong, M., Kim, M., Jang, H., Bo, S., Deepa, P., Sowndhararajan, K., Kim, S. (2023): Multivariate analysis of essential oil composition of Artemisia annua L. collected from different locations in Korea. Molecules, 28: 1131. DOI: 10.3390/molecules28031131
  32. Hussien, N.A., Al-Malki, J.S., Al-Harthy, F.A.R., Mazi, A.W., Al-Shadadi, J.A.A. (2023): Sustainable eco-friendly synthesis of zinc oxide nanoparticles using banana peel and date seed extracts: characterization and cytotoxicity evaluation. Sustainability, 15: 9864. DOI: 10.3390/su15139864
  33. Imran, M., Jan, H., Faisal, S., Shah, S.A., Shah, S., Khan, M.N., Akbar, M.T., Rizwan, M., Jan, F., Syed, S. (2021): In vitro examination of antiparasitic, anti-Alzheimer, insecticidal and cytotoxic potential of Ajuga bracteosa leaf extracts. Saudi J Biol Sci, 28: 3031 – 3036. DOI: 10.1016/j.sjbs.2021.02.044
  34. Iqbal, J., Abbasi, B.A., Yaseen, T., Zahra, S.A., Shahbaz, A., Shah, S.A., Uddin, S., Ma, X., Raouf, B., Kanwal, S., Amin, W., Mahmood, T., El-Serehy, H.A., Ahmad, P. (2021): Green synthesis of zinc oxide nanoparticles using Elaeagnus angustifolia leaf extracts and their multiple in vitro biological applications. Sci Rep, 11: 20988. DOI: 10.1038/s41598-021-99839-z
  35. Jan, H., Khan, M.A., Usman, H., Shah, M., Ansir, R., Faisal, S., Ullah, N., Rahman, L. (2020): Aquilegia pubiflora-mediated synthesis of nanoceria for diverse biomedical applications. RSC Adv, 10: 19219. DOI: 10.1039/D0RA01971B
  36. Jan, H., Shah, M., Andleeb, A., Faisal, S., Khattak, A., Rizwan, M., Drouet, S., Hano, C., Abbasi, B.H. (2021): Plant-based synthesis of zinc oxide nanoparticles using aqueous leaf extract of Aquilegia pubiflora and their antiproliferative activity against HepG2 cells. Oxid Med Cell Longev, 2021: 4786227. DOI: 10.1155/2021/4786227
  37. Jiraungkoorskul, W., Sahaphong, S., Sobhon, P., Riengrojpitak, S., Kangwanrangsan, N. (2005): Effects of praziquantel and artesunate on the tegument of adult Schistosoma mekongi harboured in mice. Parasitol Int, 54: 177 – 183. DOI: 10.1016/j.parint.2005.04.001
  38. Khalil, L.M., Azzam, A.M., Mohamed, H.A.M., Nigm, A.H., Taha, H.A., Soliman, M.I. (2018): In vitro effects of iron nanoparticles on Schistosoma mansoni adult worms and its intermediate host snail Biomphalaria alexandrina. J Egypt Soc Parasitol, 48: 363 – 368. DOI: 10.12816/0050443
  39. Khan, M.I., Shah, S., Faisal, S., Gul, S., Khan, S., Abdullah, Shah, S.A., Shah, W.A. (2022): Monotheca buxifolia-driven synthesis of zinc oxide nanomaterial: characterization and biomedical applications. Micromachines, 13: 668. DOI: 10.3390/mi13050668
  40. Khorrami, S., Zarrabi, A., Khaleghi, M., Danaei, M., Mozafari, M.R. (2018): Selective cytotoxicity of green-synthesized silver nanoparticles against MCF-7 cells and enhanced antioxidant and antimicrobial properties. Int J Nanomedicine, 13: 8013 – 8024. DOI: 10.2147/IJN.S189295
  41. Lenzi, H.L., Kimmel, E., Schechtman, H., Pelajo-Machado, M., Romanha, W.S., Pacheco, R.G., Mariano, M., Lenzi, J.A. (1998): Histoarchitecture of schistosomal granuloma development and involution: morphogenetic and biomechanical approaches. Mem Inst Oswaldo Cruz, 93(Suppl 1): 141 – 151. DOI: 10.1590/S0074-02761998000700020
  42. Miu, B.A., Dinischiotu, A. (2022): New green approaches in nanoparticle synthesis: an overview. Molecules, 27: 6472. DOI: 10.3390/molecules27196472
  43. Mostafa, O.M.S. (2005): Effects of sedr honey and/or black-seed oil on Schistosoma mansoni in albino mice: parasitological, biochemical and scanning electron microscopical studies. Egypt J Zool, 45: 449 – 469.
  44. Moustafa, M.A., Mossalem, H.S., Sarhan, R.M., Abdel-Rahman, A.A., Hassan, E.M. (2014): Effects of silver and gold nanoparticles as molluscicides and cercaricides on Schistosoma mansoni. Parasitol Res, 117: 3867 – 3880. DOI: 10.1007/s00436-018-6093-2
  45. Naiel, B., Fawzy, M., Halmy, M.W.A., Mahmoud, A.D. (2022): Green synthesis of zinc oxide nanoparticles using Limonium pruinosum extract: anticancer, antimicrobial and antioxidant activities. Sci Rep, 12: 20370. DOI: 10.1038/s41598-022-24805-2
  46. Pandit, C., Roy, A., Ghotekar, S., Khusro, A., Islam, M.N., Emran, T.B., Lam, S., Khandaker, M.U., Bradley, D.A. (2022): Biological agents for nanoparticle synthesis and their applications. J King Saud Univ Sci, 34: 101869. DOI: 10.1016/j.jksus.2022.101869
  47. Perumal, P., Sathakkathulla, N.A., Kumaran, K., Ravikumar, R., Selvaraj, J.J., Nagendran, V., Gurusamy, M., Shaik, N., Prabhakaran, S.G., Palanichamy, V.S., Ganesan, V., Thiraviam, P.P., Gunalan, S., Rathinasamy, S. (2024): Green synthesis of zinc oxide nanoparticles using aqueous extract of shilajit and anticancer activity against HeLa cells. Sci Rep, 14: 2204. DOI: 10.1038/s41598-024-52217-x
  48. Salvador-Recatalà, V., Greenberg, R.M. (2012): Calcium channels of schistosomes: unresolved questions and unexpected answers. Wiley Interdiscip Rev Membr Transp Signal, 1: 85 – 93. DOI: 10.1002/wmts.19
  49. Sankian, M., Talebi, F., Moghadam, M., Vahedi, F., Azad, F.A., Varasteh, A.-R. (2011): Molecular cloning and expression of cucumisin (Cuc m 1), a subtilisin-like protease of Cucumis melo in Escherichia coli. Allergol Int, 60: 61 – 67. DOI: 10.2332/allergolint.10-OA-0195
  50. Sivaraman, D., Panneerselvam, P., Muralidharan, P., Prabhu, T.P., Kumar, R.V. (2013): Green synthesis, characterization and antimicrobial activity of silver nanoparticles produced using Ipomoea aquatica leaf extract. Int J Pharm Sci Res, 4: 2280 – 2285. DOI: 10.13040/IJPSR.0975-8232.4(6).2280-85
  51. Slezakova, S., Ruda-Kucerova, J. (2017): Anticancer activity of artemisinin and its derivatives. Anticancer Res, 37: 5995 – 6003. DOI: 10.21873/anticanres.12046
  52. Taher, F.A., Ibrahim, S.A., Abd El-Aziz, A., Abou El-Nour, M.F., El-Sheikh, M.A., El-Husseiny, N., Mohamed, M.M. (2019): Antiproliferative effect of chitosan nanoparticles extracted from Procambarus clarkii against human breast cancer cell lines. Int J Biol Macromol, 131: 478 – 487. DOI: 10.1016/j.ijbiomac.2018.12.151
  53. Tekwu, E.M., Anyan, W.K., Boamah, D., Baffour-Awuah, K.O., Tekwu, S.K., Beng, V.P., Nyarko, A.K., Bosompem, K.M. (2016): Mechanically produced schistosomula as higher-throughput tools for phenotypic prescreening in drug sensitivity assays. Biomark Res, 4: 21. DOI: 10.1186/s40364-016-0075-2
  54. Wang, D., Cui, L., Chang, X., Guan, D. (2020): Biosynthesis and characterization of zinc oxide nanoparticles from Artemisia annua and investigation of their effects on proliferation, osteogenic differentiation and mineralization in human osteoblast-like MG-63 cells. J Photochem Photobiol B, 202: 111652. DOI: 10.1016/j.jphotobiol.2019.111652
  55. Yin, Q., Xiang, L., Han, X., Zhang, Y., Lyu, R., Yuan, L., Chen, S. (2025): The evolutionary advantage of artemisinin production by Artemisia annua. Trends Plant Sci, 30: 213 – 226. DOI: 10.1016/j.tplants.2024.09.006
  56. Younis, S.S., Abou-El-Naga, I.F., Radwan, K.H. (2023): Molluscicidal effect of green-synthesized silver nanoparticles using Azadirachta indica on Biomphalaria alexandrina snails and Schistosoma mansoni cercariae. Asian Pac J Trop Biomed, 13: 35 – 44. DOI: 10.4103/2221-1691.367688
DOI: https://doi.org/10.2478/helm-2025-0036 | Journal eISSN: 1336-9083 | Journal ISSN: 0440-6605
Language: English
Page range: 304 - 320
Submitted on: Jul 30, 2025
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Accepted on: Jan 13, 2026
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Published on: Dec 31, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open

© 2025 S. S. Eldera, S. Aldawsari, M. Abou El-Nour, published by Slovak Academy of Sciences, Institute of Parasitology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.