RNA-seq Analysis of Spaceflown Mouse Lungs Reveals Changes in Circadian Gene Expression
References
- Alberg A, Nonemaker J (2008). Who Is at High Risk for Lung Cancer? Population-Level and Individual-Level Perspectives. Seminars in Respiratory and Critical Care Medicine. 2008 Jun; 29(3): 223–32. doi: 10.1055/s-2008-1076742.
- Anand P, Kunnumakkara AB, Sundaram C, Harikumar KB, Tharakan ST, Lai OS, Sung B, Aggarwal BB (2008). Cancer is a preventable disease that requires major lifestyle changes. Pharm Res. 2008 Sep; 25(9): 2097–116. doi: 10.1007/s11095-008-9661-9.
- Ashok Kumar PV, Dakup MP, Sarkar S, Modasia JB, Motzner MS, Gaddameedhi S (2019). It's About Time: Advances in Understanding the Circadian Regulation of DNA Damage and Repair in Carcinogenesis and Cancer Treatment Outcomes. Yale Journal of Biology and Medicine. 2019 Jun 27; 92(2): 305–16. PMID: 31249491; PMCID: PMC6585512.
- Borrego-Soto G, Ortiz-López R, Rojas-Martínez A (2015). Ionizing radiation-induced DNA injury and damage detection in patients with breast cancer. Genetics and Molecular Biology. 2015 Nov 24; 38(4): 420–32. doi: 10.1590/S1415-475738420150019.
- Cucinotta FA (2014). Space radiation risks for astronauts on multiple International Space Station Missions. PLoS ONE. 2014; 9(4). doi:10.1371/journal.pone.0096099.
- DESeq2. Differential gene expression analysis based on the negative binomial distribution. Bioconductor. doi: 10.18129/B9.bioc.DESeq2.
- Die Trill M (2013). Anxiety and sleep disorders in cancer patients. EJC Suppl. 2013 Sep; 11(2): 216–24. doi: 10.1016/j.ejcsup.2013.07.009. PMID: 26217130; PMCID: PMC4041166.
- Eckel-Mahan K, Sassone-Corsi P (2009). Metabolism control by the circadian clock and vice versa. Nature Structural & Molecular Biology. 2009 May; 16(5): 462–7. doi: 10.1038/nsmb.1595.
- Galazka, JM, Lai Polo, S-H, Saravia-Butler, AM, Fogle, HW, Bense, N, Dinh, MT, Costes, SV, & Gebre, SG. NASA OSDR: Open Science for Life in Space [Internet]. osdr.nasa.gov. [cited 2023 Aug 30]. Available from:
https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-248/ . - Gridley DS, Mao XW, Tian J, Cao JD, Perez C, Stodieck LS, Ferguson VL, Bateman TA, Pecaut MJ (2015). Genetic and Apoptotic Changes in Lungs of Mice Flown on the STS-135 Mission in Space. In Vivo. 2015 Jul-Aug; 29(4): 423–33. PMID: 26130787.
- Guo JH, Qu WM, Chen SG, Chen XP, Lv K, Huang ZL, et al (2014). Keeping the right time in space: importance of circadian clock and sleep for physiology and performance of astronauts. Military Medical Research. 2014; 1(1): 23. doi: 10.1186/2054-9369-1-23.
- Guo Z, Zhou G, Hu W. Carcinogenesis induced by space radiation: A systematic review (2022). Neoplasia. 2022 Oct; 32: 100828. doi: 10.1016/j.neo.2022.100828.
- Heiney PA. 8.2 - Biological Effects of Ionizing Radiation [Internet]. UPenn; 2016 [cited 2023 Aug 28]. Available from:
https://repository.upenn.edu/entities/publication/6ed9f583-7fbe-4030-811fe47ef92d9e45 . - Kim TW, Jeong JH, Hong SC. The impact of sleep and circadian disturbance on hormones and metabolism. Int J Endocrinol. 2015;2015:591729. doi: 10.1155/2015/591729. Epub 2015 Mar 11. PMID: 25861266; PMCID: PMC4377487.
- Lahti T, Merikanto I, Partonen T (2012). Circadian clock disruptions and the risk of cancer. Annals of Medicine. 2012 Oct 16; 44(8): 847–53. doi: 10.3109/07853890.2012.727018.
- Li HX. The role of circadian clock genes in tumors. OncoTargets and Therapy. 2019 May; Volume 12: 3645–60. doi: 10.2147/OTT.S203144.
- Liu Z, Yu K, Zheng J, Lin H, Zhao Q, Zhang X, et al (2019). Dysregulation, functional implications, and prognostic ability of the circadian clock across cancers. Cancer Medicine. 2019; 8(4): 1710–20. doi:10.1002/cam4.2035.
- Mithoowani M, Febbraro M (2022). Non-Small-Cell Lung Cancer in 2022: A Review for General Practitioners in Oncology. Curr. Oncol. 29: 1828–1839. doi:10.3390/curroncol29030150.
- NASA. NASA GeneLab Data Systems: Galaxy. NASA [Internet]. [cited 2023 Aug 30]. Currently decommissioned, now found at
https://usegalaxy.org/ . - Papagiannakopoulos T, Bauer Matthew R, Davidson Shawn M, Heimann M, Subbaraj L, Bhutkar A, et al (2016). Circadian Rhythm Disruption Promotes Lung Tumorigenesis. Cell Metabolism. 2016 Aug; 24(2): 324–31. doi: 10.1016/j.cmet.2016.07.001.
- Perez J. Why Space Radiation Matters [Internet]. NASA. NASA; 2019 [cited 2023 Aug 30]. Available from:
https://www.nasa.gov/analogs/nsrl/why-space-radiation-matters . - Prisk GK (2014). Microgravity and the respiratory system. European Respiratory Journal. 2014; 43(5): 1459–71. doi: 10.1183/09031936.00001414.
- Rijo-Ferreira F, Takahashi JS (2019). Genomics of circadian rhythms in health and disease. Genome Medicine. 2019 Dec; 11(1). doi: 10.1186/s13073-019-0704-0.
- Rudin CM, Brambilla E, Faivre-Finn C, Sage J (2021). Small-cell lung cancer. Nature Reviews Disease Primers 2021 Jan 14; 7(1): 1–20. doi: 10.1038/s41572-020-00235-0.
- SEER Cancer Stat Facts: Lung and Bronchus Cancer. National Cancer Institute. 2023.
- The National Academies Press. Health Risks from Exposure to Low Levels of Ionizing Radiation: BEIR VII Phase 2. NAP.edu. nap. nationalacademies.org. [cited 2023 Aug 30].
- The National Academies Press. Review of NASA's Longitudinal Study of Astronaut Health. NAP.edu [Internet]. nap.nationalacademies. org. [cited 2023 Aug 30]. Available from:
https://nap.nationalacademies.org/read/10903/chapter/4 .
DOI: https://doi.org/10.2478/gsr-2026-0004 | Journal eISSN: 2332-7774
Language: English
Page range: 28 - 37
Published on: Jun 12, 2026
Published by: American Society for Gravitational and Space Research
In partnership with: Paradigm Publishing Services
Publication frequency: Volume open
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© 2026 Anika Kulkarni, Emily Tu, Rajasi Kolhatkar, Jennifer Claudio, Sigrid Reinsch, Elizabeth A. Blaber, published by American Society for Gravitational and Space Research
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.