Have a personal or library account? Click to login
Verification of the presence of antibiotic residue in sheep meat Cover

Verification of the presence of antibiotic residue in sheep meat

Open Access
|Dec 2025

References

  1. 1. Regulation (EU) 2019/6 of the European Parliament and of the Council of 11 December 2018 on veterinary medicinal products and repealing Directive 2001/82/EC. OJ L4, 43–167.
  2. 2. Hao, H., Cheng, G., Iqbal, Z., Ai, X., Hussain, H. I., Huang, L., Dai, M., Wang, Y., Liu, Z., Yuan, Z., 2014: Benefits and risks of antimicrobial use in food-producing animals. Front. Microbiol., 5. 288, 1–11. DOI: 10.3389/fmicb.2014.00288
  3. 3. Beyene, T., 2015: Veterinary drug residues in food-animal products: Its risk factors and potential effects on public health. J. Vet. Sci. Technol., 7, 1, 1–7.
  4. 4. Okocha, R. C., Olatoye, I. O., Adedeji, O. B., 2018: Food safety impacts of antimicrobial use and their residues in aquaculture. Public Health Rev., 39, 21, 1–22. DOI: 10.1186/s40985-018-0099-2
  5. 5. Regulation (EC) No 470/2009 of the European Parliament and of the Council of 6 May 2009 laying down Community procedures for the establishment of residue limits of pharmacologically active substances in foodstuffs of animal origin, repealing Council Regulation (EEC) No. 2377/90 and amending Directive 2001/82/EC of the European Parliament and of the Council Regulation (EC) No. 726/2004 of the European Parliament and the Council. OJ L152, 1–22.
  6. 6. Commission Regulation (EU) No 37/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. OJ L15, 1‒72.
  7. 7. Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official controls and other official activities performed to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant protection products, repealing Regulations (EC) No 854/2004 and (EC) No 882/2004 of the European Parliament and of the Council. OJ L95, 1–142.
  8. 8. Commission Delegated Regulation (EU) 2022/1644 of 7 July 2022 supplementing Regulation (EU) 2017/625 of the European Parliament and of the Council with specific requirements for the performance of official controls on the use of pharmacologically active substances authorised as veterinary medicinal products or as feed additives and of prohibited or unauthorised pharmacologically active substances and residues thereof. OJ L 248, 3–17.
  9. 9. Commission Implementing Regulation (EU) 2022/1646 of 23 September 2022 on uniform practical arrangements for the performance of official controls as regards the use of pharmacologically active substances authorised as veterinary medicinal products or as feed additives and of prohibited or unauthorised pharmacologically active substances and residues thereof, on specific content of multi-annual national control plans and specific arrangements for their preparation. OJ L 248, 32–45.
  10. 10. Commission Implementing Regulation (EU) 2021/808 of 22 March 2021 on the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results as well as on the methods to be used for sampling and repealing Decisions 2002/657/EC and 98/179/EC. OJ L180, 84–109.
  11. 11. European Commission, 2021: EURL Guidance Document on Confirmatory Method Validation, Version 1.1, November 25, 2021 [online]. Available at: https://food.ec.europa.eu/system/files/2022-06/cs_vet-med-residues_guideline_eurl_validation-confirmatory.pdf
  12. 12. Kožárová, I., Juščáková, D., Šimková, J., Milkovičová, M., Kožár, M., 2020: Effective screening of antibiotic and coccidiostat residues in food of animal origin by reliable broad-spectrum residue screening tests. Ital. J. Anim. Sci., 19, 1, 487–501. DOI: 10.1080/1828051X.2020.1761270
  13. 13. Gaudin, V., Rault, A., Hedou, C., Soumet, C., Verdon, E., 2017: Strategies for the screening of antibiotic residues in eggs: comparison of the validation of the classical microbiological method with an immunobiosensor method. Food Addit. Contam. Part A, 34, 9, 1510–1527. DOI: 10.1080/19440049.2017.1339331
  14. 14. Li, S., Zhang, Q., Chen, M., Zhang, X., Liu, P., 2020: Determination of veterinary drug residues in food of animal origin: Sample preparation methods and analytical techniques. J. Liq. Chromatogr. Relat. Technol., 43, 17–18, 701–724. DOI: 10.1080/10826076.2020.1798247
  15. 15. European Commission, 2023: EURL Guidance Document on Screening Method Validation, Version 1.1, September 21, 2023 [online]. Available at: https://sitesv2.anses.fr/en/system/files/Guidance_screening_20230921_v1_0.pdf
  16. 16. Cantwell, H., O’Keeffe, M., 2006: Evaluation of the Premi® Test and comparison with the One-Plate Test for the detection of antimicrobials in kidney. Food Addit. Contam. Part A, 23, 2, 120–125. DOI: 10.1080/02652030500357433
  17. 17. Gondová, Z., Kožárová, I., Poláková, Z., Maďarová, M., 2014: Comparison of four microbiological inhibition tests for the screening of antimicrobial residues in the tissues of food-producing animals. Ital. J. Anim. Sci., 13, 4, 728–734. DOI: 10.4081/ijas.2014.3521
  18. 18. Pikkemaat, M. G., 2009: Microbial screening methods for detection of antibiotic residues in slaughter animals. Anal. Bio-anal. Chem., 395, 893–905. DOI: 10.1007/s00216-009-2841-6
  19. 19. Sanz, D., Razquin, P., Condón, S., Juan, T., Herraiz, B., Mata, L., 2015: Incidence of antimicrobial residues in meat using a broad-spectrum screening strategy. Eur. J. Nutr. Food Saf., 5, 3, 156–165. DOI: 10.9734/EJNFS/2015/13795
  20. 20. Mata, L., Sanz, D., Razquin, P., 2014: Validation of the Explorer®2.0 test coupled to e-Reader® for the screening of antimicrobials in muscle from different animal species. Food Addit. Contam. Part A, 31, 9, 1496–1505. DOI: 10.1080/19440049.2014.934303
  21. 21. Serrano, M. J., Mata, L., García-Gonzalo, D., Antón, A., Razquin, P., Condón, S., Pagán, R., 2021: Optimization and validation of a new microbial inhibition test for the detection of antimicrobial residues in living animals intended for human consumption. Foods, 10, 8, 1897. DOI: https://doi.org/10.3390/foods10081897
  22. 22. Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. OJ L221, 8‒36.
  23. 23. Gaudin, V., Juhel-Gaugain, M., Morétain, J. P., Sanders, P., 2008: AFNOR validation of Premi®Test, a microbiological-based screening tube-test for the detection of antimicrobial residues in animal muscle tissue. Food Addit. Contam. Part A, 25, 12, 1451–1464. DOI: 10.1080/02652030802429088
  24. 24. Dang, P. K., Degand, G., Douny, C., Ton, V. D., Maghuin-Rogister, G., Scippo, M., 2011: Optimisation of a new two-plate test screening method for the detection of antibiotic residues in meat. Int. J. Food Sci. Tech., 46, 10, 2070‒2076. DOI: 10.1111/j.1365-2621.2011.02718.x
  25. 25. Gaudin, V., Hedou, C., Rault, A., Verdon, E., 2010: Validation of a Five Plate Test, the STAR protocol, for the screening of antibiotic residues in muscle from different animal species according to European Decision 2002/657/EC. Food Addit. Contam. Part A, 27, 7, 935–952. DOI: 10.1080/19440041003677483
  26. 26. Menkem, Z. O. E., Ngangom, B. L., Tamunjoh, S. S. A., Boyom, F. F., 2019: Antibiotic residues in food animals: Public health concern. Acta Ecol. Sin., 39, 5, 411–415. DOI: 10.1016/j.chnaes.2018.10.004
  27. 27. Bacanli, M., Başaran, N., 2019: Importance of antibiotic residues in animal food. Food Chem. Toxicol,, 125,462–466. DOI: 10.1016/j.fct.2019.01.033
  28. 28. Parthasarathy, R., Monette, C. E., Bracero, S., Saha, M. S., 2018: Methods for field measurement of antibiotic concentrations: limitations and outlook. FEMS Microbiol. Ecol., 94, 8, 1–10 DOI: 10.1093/femsec/fiy105
  29. 29. Gaudin, V., Hedou, C., Verdon, E., 2009: Validation of a wide-spectrum microbiological tube test, the EXPLORER® test, for the detection of antimicrobials in muscle from different animal species. Food Addit. Contam. Part A, 26, 8, 1162–1171. DOI: 10.1080/02652030902939671
  30. 30. The Department of Agriculture, Food and the Marine, 2023: National Risk-based Residue Control plan report, Ireland, 1-14. Available at: https://assets.gov.ie/static/documents/National_Residue_Control_Plan_Report_2023_260625.pdf
DOI: https://doi.org/10.2478/fv-2025-0040 | Journal eISSN: 2453-7837 | Journal ISSN: 0015-5748
Language: English
Page range: 85 - 94
Submitted on: Nov 13, 2025
|
Accepted on: Dec 1, 2025
|
Published on: Dec 23, 2025
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Ivona Kožárová, Deboragh Howard, Simona Hriciková, published by The University of Veterinary Medicine and Pharmacy in Košice
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.