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Pre-sowing treatment of acorns with gibberellic acid and N-hexanoyl-L-homoserine lactone induced changes in growth and hormonal balance of Quercus robur L. seedlings Cover

Pre-sowing treatment of acorns with gibberellic acid and N-hexanoyl-L-homoserine lactone induced changes in growth and hormonal balance of Quercus robur L. seedlings

Forestry Studies
Volume 80 (2024): Issue 1 (December 2024)
By: Lesya Voytenko,  Valentyna Vasyuk,  Lidia Babenko,  Mykola Shcherbatiuk,  Kateryna Romanenko and  Iryna V. Kosakivska  
Open Access
|Dec 2024

Figures & Tables

Figure 1.

Effect of pre-sowing treatment with GA3 (50 mg/L) and C6-HSL (300 ng/L) solutions on Quercus robur acorns' germination (47 days, %). Significance at ** - P <0.01 compared with the control on the 47th day of germination; n = 60; the bars represent the standard error (±SE).
Effect of pre-sowing treatment with GA3 (50 mg/L) and C6-HSL (300 ng/L) solutions on Quercus robur acorns' germination (47 days, %). Significance at ** - P <0.01 compared with the control on the 47th day of germination; n = 60; the bars represent the standard error (±SE).

Figure 2.

Seedlings of Quercus robur for 47 days of vegetation in laboratory conditions, grown from non-primed acorns (C-plants); acorns primed with GA3 (GA3+plants) and with C6-HSL (C6-HSL+plants).
Seedlings of Quercus robur for 47 days of vegetation in laboratory conditions, grown from non-primed acorns (C-plants); acorns primed with GA3 (GA3+plants) and with C6-HSL (C6-HSL+plants).

Figure 3.

Morphological characteristics of 47-day-old Quercus robur seedlings grown from non-primed acorns (C-plants); acorns primed with GA3 (GA3+plants) and with C6-HSL (C6-HSL+plants). a, b – height and fresh weight of shoots, c, d – length and fresh weight of roots. Significance at * - P <0.05 and ** - P <0.01 compared with the control on the 47th day of acorn germination; n = 30; the bars represent the standard error (±SE).
Morphological characteristics of 47-day-old Quercus robur seedlings grown from non-primed acorns (C-plants); acorns primed with GA3 (GA3+plants) and with C6-HSL (C6-HSL+plants). a, b – height and fresh weight of shoots, c, d – length and fresh weight of roots. Significance at * - P <0.05 and ** - P <0.01 compared with the control on the 47th day of acorn germination; n = 30; the bars represent the standard error (±SE).

Figure 4.

Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solution on the accumulation and distribution of endogenous ABA, IAA, GA3 and GA4 in 47-day-old Quercus robur seedlings. Significance at * - P <0.05, ** - P <0.01 and *** - P <0.001 compared with the control on the 47th day of acorn germination; n = 9; the bars represent the standard error (±SE).
Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solution on the accumulation and distribution of endogenous ABA, IAA, GA3 and GA4 in 47-day-old Quercus robur seedlings. Significance at * - P <0.05, ** - P <0.01 and *** - P <0.001 compared with the control on the 47th day of acorn germination; n = 9; the bars represent the standard error (±SE).

Figure 5.

Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solution on endogenous SA content in 47-day-old Quercus robur seedlings. Significance at * - P <0.05 compared with the control on the 47th day of acorn germination; n = 9; the bars represent the standard error (±SE).
Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solution on endogenous SA content in 47-day-old Quercus robur seedlings. Significance at * - P <0.05 compared with the control on the 47th day of acorn germination; n = 9; the bars represent the standard error (±SE).

Figure 6.

Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solutions on the content and distribution of endogenous cytokinins in 47-day-old Quercus robur seedlings. Designation: trans-zeatin (t-Z), trans-zeatin riboside (t-ZR), trans-zeatin-O-glucoside (t-ZOG) isopentenyladenine (iP), and isopentenyladenosine (iPA). Significance at * - P <0.05 and ** - P <0.01 compared with the control on the 47th day of acorn germination; n = 9; the bars represent the standard error (±SE).
Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solutions on the content and distribution of endogenous cytokinins in 47-day-old Quercus robur seedlings. Designation: trans-zeatin (t-Z), trans-zeatin riboside (t-ZR), trans-zeatin-O-glucoside (t-ZOG) isopentenyladenine (iP), and isopentenyladenosine (iPA). Significance at * - P <0.05 and ** - P <0.01 compared with the control on the 47th day of acorn germination; n = 9; the bars represent the standard error (±SE).

Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solutions on the total content of endogenous cytokinins in the shoots and roots of 47-day-old Quercus robur seedlings, ng/g FW_

Option experimentC-plantsGA3 +plantsC6-HSL + plants
Shoots49.5±2.546.7*±2.352.8*±2.6
Roots16.7±0.843.1**±2.236.5*±1.8

Effect of pre-sowing treatment of acorns with GA3 (50 mg/L) and C6-HSL (300 ng/L) solutions on the accumulation of dry weight (mg) and water content (%) in 47-day-old Quercus robur seedlings' shoots and roots_

IndicesC-plantsGA3+plantsC6-HSL+plants
ShootsRootsShootsRootsShootsRoots
Dry weight, mg60±3.1162±8.1106**±5.3171**±8.6111±5.6225***±11.3
Water content, %86.675.880.878.176.872.5

Effect of pre-sowing treatment of acorns with GA3 and C6-HSL solutions on the total content of endogenous phytohormones in 47-day-old Quercus robur seedlings, ng/g FW_

Option experimentControlGA3 +plantsC6-HSL + plants
ABA43.7±2.1852.1*±2.6179.7*±3.98
IAA27.8±1.3917.4*±0.8723.9*±1.18
GA3+GA419.8±0.9721.4±1.0830.1*±1.51
SA271.6±13.58238.3*±11.92282.3±14.12
Cytokinins66.2±3.3089.8*±4.4789.3*±4.46
DOI: https://doi.org/10.2478/fsmu-2024-0008 | Journal eISSN: 1736-8723 | Journal ISSN: 1406-9954
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Language: English
Page range: 127 - 141
Published on: Dec 31, 2024
Published by: Estonian University of Life Sciences
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
N-hexanoyl-L-homoserine lactone,
gibberellic acid,
priming,
acorn germination,
hormonal balance
Related subjects:
Life sciences,
Plant science,
Ecology,
Life sciences, other

© 2024 Lesya Voytenko, Valentyna Vasyuk, Lidia Babenko, Mykola Shcherbatiuk, Kateryna Romanenko, Iryna V. Kosakivska, published by Estonian University of Life Sciences
This work is licensed under the Creative Commons Attribution 4.0 License.

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