Effect of different water contents in the substrate on cultivation of Pleurotus ostreatus Jacq. P. Kumm

Parameter	Cultivation phase	60%		65%		70%		75%
Parameter	Cultivation phase	1	2	1	2	1	2	1	2
Water content (%)	Colonisation	59.4 ± 0.7 aA	62.63 ± 0.76 aB	72.01 ± 1.09 aA	68.75 ± 1.07 aB	72.75 ± 1.89 aA	72.37 ± 0.53 aA	79.32 ± 0.69 aA	76.82 ±0.66 aB
	Primordia	68.1 ±0.87 aA	52.37 ± 1.02 bB	67.8 ± 0.65 bA	66.08 ± 0.63 bA	70.65 ± 1.21 abA	68.95 ± 0.19 bA	78.93 ± 1.06 aA	76.55 ± 0.37 aB
	First flush	41.4 ± 0.45 bA	57.7 ±0.37 cB	58.5 ± 0.67 cA	66.55 ± 0.61 abB	68.17 ± 1.02 bA	67.28 ± 0.22 cA	74.55 ± 0.47 bA	75.0 ± 0.8 aA
pH	Colonisation	4.74 ± 0.03 aA	5.09 ±0.01 aB	4.81 ± 0.02 aA	5.22 ±0.01 aB	5.12 ± 0.12 aA	5.27 ± 0.06 aA	5.13 ± 0.07 aA	5.43 ± 0.04 aB
	Primordia	5.33 ± 0.19 bA	5.27±0 abA	5.13 ± 0.01 bA	5.31 ±0.03 bB	5.13 ± 0.06 aA	5.2 ± 0.01 aA	5.37 ± 0.06 bA	5.37 ± 0 aA
	First flush	5.29 ±0.1 bA	5.35 ±0.11 bA	5.5 ± 0.05 cA	5.63 ± 0.03 cB	5.16 ± 0.08 aA	5.26 ± 0.02 aA	5.83 ± 0.03 cA	5.63 ± 0.01 bB

1 Values are the means ± SD (n = 3).1 – upper layer of substrate, 2 – centre of substrate.

Different lowercase letters in a column indicate significant differences between stages of cultivation; capital letters indicate significant differences between layers of the substrate for each parameter (Kruskal–Wallis test, p ≤ 0.05).

Mycelial growth rate at different substrate water content. Columns represent the mean values ± SD (n = 40). Different letters indicate significant differences (Kruskal–Wallis test, p ≤ 0.05).

BE of substrate at different substrate water content. Columns represent the mean values ± SD (n = 3). Different letters indicate significant differences (Kruskal–Wallis test, p ≤ 0.05). BE, biological efficiency.

Table 2.

Changes of enzymatic activities in substrate during cultivation of Pleurotus ostreatus.

Enzymes	Cultivation phase	60%		65%		70%		75%
Enzymes	Cultivation phase	1	2	1	2	1	2	1	2
ß-D-G (μmol MUFG · h⁻¹ · g⁻¹)	Colonisation	474.88 ± 153.2 aA	221.65 ± 27.75 aA	275.17 ±99.17 aA	560.2 ± 176.26 aA	717.26 ±45.31 aA	508.59 ±47.71 aB	184.77 ± 14.27 aA	260.08 ± 23.03 aB
	Primordia	252.16 ± 78.34 aA	219.69 ±6.63 aA	1046.78 ± 10.52 bA	417.16 ± 18.37 aB	332.68 ±34.07 bA	125.93 ±25.27 bB	382.05 ±35.24 aA	278.41 ± 91.8 aA
	First flush	236.59 ±30.11 aA	570.39 ± 27.34 bB	843.88 ± 5.23 cA	691.2 ± 31.11 aB	678.78 ± 12.87 aA	290.46 ± 15.24 cB	3349.13 ± 549.5 bA	4083.42 ± 608.2 bA
Cell, (μmol MUFC ± h⁻¹ · g⁻¹)	Colonisation	96.03 ± 9.69 aA	24.06 ± 4.03 aB	34.68 ± 6.18 aA	40.53 ± 12.42 aA	108.3 ± 28.03 aA	61.81 ±4.08 aA	17.93 ± 11.54 aA	13.61 ± 5.07 aA
	Primordia	34.56 ± 19.44 bA	33.3 ± 15.28 aA	110.17 ±27.99 bA	18.37 ± 8.37 aB	24.7 ± 2.61 bA	5.74 ± 0.3 bA	31.59 ± 7.79 aA	20.13 ± 5.9 aA
	First flush	30.74 ± 5.77 bA	59.15 ± 31.45 aA	85.59 ± 26.23 abA	71.47 ±8.49 bA	110.23 ±31.02 aA	25.57± 1.11 cB	931.88 ± 343.3 bA	1306.13 ± 387.54 bA
Mn – P (mU · g⁻¹)	Colonisation	1.38 ±0.01 aA	2.39 ±0.91 aA	l.l8±O.54aA	1.2 ±0.64 aA	1.27 ±0.09 aA	1.13 ±0.42 aA	1.49 ±0.09 aA	1.79 ± 0.13 aA
	Primordia	0.96 ±0.37 aA	1.1 ±0.03 aA	1.1 ±0.3 aA	0.96 ± 0.12 aA	0.12 ±0.13 aA	1.07 ±0.24 aB	1.4 ± 0.17 aA	1.31 ±0.04 bB
	First flush	0.09 ±0.02 bA	1.55 ±0.03 aB	0.92 ± 0.24 aA	0.62 ±0.24 aA	0.92 ±0.34 aA	0.55 ± 0.14 aA	0.76 ± 0.2 bA	0.53 ± 0.34 bA

1 Values are the means ± SD (n = 3). 1 – upper layer of substrate, 2 – centre of substrate.

MUFC, 4-methylumbellyferyl-N-cellobiopyranoside; MUFG, 4-methylumbellyferyl-β-D-glucopyranoside.

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.2478/fhort-2023-0002 | Journal eISSN: 2083-5965 | Journal ISSN: 0867-1761

Journal RSS Feed

Language: English

Page range: 25 - 31

Submitted on: Oct 31, 2022

Accepted on: Jan 2, 2023

Published on: Jun 26, 2023

Published by: Polish Society for Horticultural Sciences (PSHS)

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

enzymes,

pH,

Related subjects:

© 2023 Lucie Wiesnerová, Tereza Hřebečková, Ivan Jablonský, Martin Koudela, published by Polish Society for Horticultural Sciences (PSHS)
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 35 (2023): Issue 1 (June 2023)

Effect of different water contents in the substrate on cultivation of Pleurotus ostreatus Jacq. P. Kumm

Figures & Tables

Table 1.

Figure 1.

Figure 2.

Table 2.

Paradigm

My account