Bestimmung der Endophyten im Blutungssaft der Rebe mittels Next Generation Sequencing

Karin Mandl; Jasmina Suljic; Christian Bader; Ingrid Hofstetter; Florian Faber

doi:10.2478/boku-2022-0009

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Bestimmung der Endophyten im Blutungssaft der Rebe mittels Next Generation Sequencing

Die Bodenkultur: Journal of Land Management, Food and Environment

Volume 73 (2022): Issue 2 (June 2022)

By: Karin Mandl, Jasmina Suljic, Christian Bader, Ingrid Hofstetter and Florian Faber

Open Access

|Mar 2023

Abstract

The grapevine represents a natural reservoir of resident microbial resources embedded in a complex microecosystem. The aim of this study was to find out which germs are present in the bleeding sap. The bleeding juice was obtained using a clean with alcohol sterilized PET bottle. After enrichment, DNA extraction followed by analysis of an Illumina MiSeq v3 with target region V1V3 was examined. The sequences obtained were aligned with the NCBI database. The most dominant genera in the grapevines were Pseudomonas and Massilia followed by the genera Zoogloea, Bacillus, Idonella, Sphingomonas and Paenibacillus. Sporadically, other bacterial genera appeared in the vines. In addition, the yeast-like microorganism Aureobasidium pullulans was determined in two vines and the described inhibitory interaction between Pseudomonas and Aureobasidium was confirmed as described in the literature. In general, all microorganisms determined in the bleeding sap have a plant-strengthening influence and provide a basis for colonization into tissue-specific plant parts.

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Articles in this issue

DOI: https://doi.org/10.2478/boku-2022-0009 | Journal eISSN: 2719-5430 | Journal ISSN: 0006-5471

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Language: English, German

Page range: 123 - 133

Submitted on: Aug 12, 2022

Accepted on: Oct 9, 2022

Published on: Mar 9, 2023

Published by: Universität für Bodenkultur Wien

In partnership with: Paradigm Publishing Services

Publication frequency: Volume open

Keywords:

Aureobasidium pullulans

Related subjects:

Life sciences,

Ecology,

Life sciences, other

© 2023 Karin Mandl, Jasmina Suljic, Christian Bader, Ingrid Hofstetter, Florian Faber, published by Universität für Bodenkultur Wien
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 73 (2022): Issue 2 (June 2022)