Enzyme-Assisted Extraction of Polyphenols From Rose (Rosa Damascena Mill.) Petals

Kalcheva-Karadzhova, Krasimira; Shikov, Vasil; Mihalev, Kiril; Dobrev, Georgi; Ludneva, Danka; Penov, Nikolai

doi:10.2478/aucft-2014-0015

Abstract

: The efficiency of enzyme-assisted extraction for the recovery of polyphenols from rose (Rosa damascena Mill.) petals was evaluated performing a simplex centroid experimental design for mixture with three components (pectinolytic, cellulolytic and hemicellulolytic preparation). The ternary enzyme combinations leaded to the highest contents of total polyphenols, reaching 43% higher average value as compared to the control (without enzymatic treatment) sample. Enzymatic treatments also enhanced (9−25%) the extractability of total anthocyanins. The results obtained demonstrate that enzyme-assisted extraction improves the recovery of polyphenolic antioxidants from rose petals, especially using ternary enzyme combinations, comprising pectinolytic, cellulolytic and hemicellulolytic preparation.

References

1. Anesini C. & Perez C. (1993). Screening of plants used in Argentine folk medicine for antimicrobial activity. J. Ethnopharmacol., 39, 119−128. DOI: 10.1016/0378-8741(93)90027-3.10.1016/0378-8741(93)90027-3
Search in Google Scholar
2. Benzie I.F.F. & Strain J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70−76. DOI: 10.1006/abio.1996.0292.10.1006/abio.1996.0292
Search in Google Scholar
3. Brand-Williams W., Cuvelier M.E. & Berset C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25−30. DOI: 10.1016/S0023-6438(95)80008-5.10.1016/S0023-6438(95)80008-5
Search in Google Scholar
4. Cho E.J., Yokozawa T., Rhyu D.Y., Kim S.C., Shibahara N. & Park J.C. (2003). Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical, Phytomedicine, 10(6-7), 544−551. DOI: 10.1078/094471103322331520.10.1078/094471103322331520
Search in Google Scholar
5. Choi E.M. & Hwang J.K. (2003). Investigations of anti-inflammatory and antinociceptiveactivities of Piper cubeba, Physalis angulata and Rosa hybrida. J. Ethnopharmacol., 89(1), 171−175. DOI: 10.1016/S0378-8741(03)00280-0.10.1016/S0378-8741(03)00280-0
Search in Google Scholar
6. Dinkova R., Shikov, V., Mihalev K., Velchev Z., Dinkov H. & Mollov P. (2012). Changes in the total anthocyanins and polyphenols during processing of wild berries into freshly pressed juices. Journal of EcoAgriTourism, 8(1), 254−259.
Search in Google Scholar
7. Dixit N., Tripathi C. & Upadhyay R. (1976). The antifungal substance of rose flowers (Rosa indica). Econ. Bot., 30, 371−374. DOI:10.1007/BF02904658.10.1007/BF02904658
Search in Google Scholar
8. Dobrev G. & Zhekova B. (2012). Biosynthesis, purification and characterization of endodlucanase from xylanase, produsing strain Aspergilus niger B03. Brazilian Journal of Microbiology, 43(1), 70−77. DOI: 10.1590/S1517-83822012000100008.10.1590/S1517-83822012000100008376897424031805
Search in Google Scholar
9. Dobrev G., Pishtiyski I., Stanchev V. & Mircheva R. (2007), Optimization of nutrient medium containing agricultural wastes for xylanase production by Aspergillus niger B03 using optimal composite design. Bioresource Technology, 98(14), 2671−2678. DOI:10.1016/j.biortech.2006.09.022.10.1016/j.biortech.2006.09.02217092711
Search in Google Scholar
10. Fenglin H., Ruili L., Bao H. & Liang M. (2004). Free radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants. Fitoterapia, 75(1), 14−23. DOI: 10.1016/j.fitote.2003.07.003.10.1016/j.fitote.2003.07.00314693215
Search in Google Scholar
11. Ginova A., Mihalev K. & Kondakova V. (2013). Antioxidant capacity of petals and leaves from different rose (Rosa damascena Mill.) plantations in Bulgaria. International Journal of Pure Applied Bioscience, 1, 38−43.
Search in Google Scholar
12. Giusti M.M. & Wrolstad R.E. (2001). Characterization and measurement of anthocyanins by UV-visible spectroscopy. In R.E. Wrolstad (Ed.), Current Protocols in Food Analytical Chemistry (pp. F1.2.1−F1.2.13), New York: Wiley.
Search in Google Scholar
13. Kaverzneva E.D. (1971). Assay for determination of proteolytic activity of complex enzymes and proteinases. Prikladnaja biochimia i Mikrobiologia, 7, 225−228 (in Rusian).
Search in Google Scholar
14. Magalhães L.M., Segundo M.A., Reis S. & Lima J.L.F.C. (2008). Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta, 613(1), 1−19. DOI: 10.1016/j.aca.2008.02.04710.1016/j.aca.2008.02.04718374697
Search in Google Scholar
15. Mahmood N., Piacente S., Pizza C., Burke A., Khan A.I. & Hay A.J. (1996). The anti-HIVactivity and mechanisms of action of pure compounds isolated from Rosa damascena. Biochem. Biophys. Res. Comm., 229(1), 73−79. DOI: 10.1006/bbrc.1996.1759.10.1006/bbrc.1996.1759
Search in Google Scholar
16. Miller G.L. (1959). Use of dinitrosalicyclic acid reagent for determination of reducing sugar. J. Anal. Chem., 31(3), 426−428. DOI: 10.1021/ac60147a030.10.1021/ac60147a030
Search in Google Scholar
17. Miron T.L., Herrero M. & Ibáñez E. (2013). Enrichment of antioxidant compounds from lemon balm (Melissa officinalis) by pressurized liquid extraction and enzyme-assisted extraction. Journal of Chromatography A, 1288(3), 1−9. DOI: 10.1016/j.chroma.2013.02.075.10.1016/j.chroma.2013.02.075
Search in Google Scholar
18. Ng T.B., He J.S., Niu S.M., Zhao L., Pi Z.F., Shao W.& Liu F. (2004). A gallic acid derivative and polysaccharides with antioxidative activity from rose (Rosa rugosa) flowers. J. Pharm. Pharmacol., 56, 537−545. DOI: 10.1211/0022357022944.10.1211/0022357022944
Search in Google Scholar
19. Perez C. & Anesini C. (1994). In vitro antibacterial activity of Argentine folk medicinal plants against Salmonella typhi. J. Ethnopharmacol., 44(1), 41−46. DOI: 10.1016/0378-8741(94)90097-3.10.1016/0378-8741(94)90097-3
Search in Google Scholar
20. Pinelo-Jiménez M. & Meyer A.S. (2008). Enzyme-assisted extraction of antioxidants: Release of phenols from vegetal matrixes. Electronic Journal of Environmental, Agricultural and Food Chemistry, 7(8), 3217−3220.
Search in Google Scholar
21. Ponpium P., Ratanakhanokchai K. & Kyu K. (2000). Isolation and properties of a cellulosome-type multienzyme complex of the thermophilic Bacteroides sp. strain P-1. Enzyme Microbial. Technol., 26(5-6), 459−465. DOI: 10.1016/S0141-0229(99)00195-7.10.1016/S0141-0229(99)00195-7
Search in Google Scholar
22. Singleton V.L. & Rossi J.A. Jr. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144−158.
Search in Google Scholar
23. VanderJagt T.J., Ghattas R., VanderJagt D.J., Crossey M. & Glew R.H. (2002). Comparison of the total antioxidant content of 30 widely used medicinal plants of New Mexico. Life Sci., 70(9), 1035−1040 DOI: 10.1016/S0024-3205(01)01481-3.10.1016/S0024-3205(01)01481-3
Search in Google Scholar
24. Vinokur Y., Rodov V., Reznick N., Goldman G., Horev B., Umiel N. & Friedman H. (2006). Rose petal tea as an antioxidant-rich beverage: cultivar effects, J. Food Sci., 71, S42−S47. DOI: 10.1111/j.1365-2621.2006.tb12404.x. 10.1111/j.1365-2621.2006.tb12404.x
Search in Google Scholar

Enzyme-Assisted Extraction of Polyphenols From Rose (Rosa Damascena Mill.) Petals

Abstract

Paradigm

My account