Effects of Fungicide and Acetylsalicylic Acid Treatments on the Physiological and Enzymatic Activity in Tomato (Lycopersicon Esculentum Mill.)

Aurelia Horotan; Simona Oancea

doi:10.2478/aucft-2013-0002

Abstract

The paper deals with investigation of growth parameters of vegetative organs, peroxidase and catalase activities of Lycopersicon esculentum Mill. treated with a systemic fungicide and acetylsalicylic acid, respectively. Our results showed that both fungicide and acetylsalicylic acid lead to growth delays in tomato stems and leaves compared to control samples. During the growth, pathogen was rapidly inhibited by tomatoes treated with acetylsalicylic acid, affecting few plants (10%) compared to control group (40%). Results showed that samples treated with acetylsalicylic acid and control samples fructified better (30% higher) than tomatoes treated with fungicide. The latter did not suffer pathogen attack, but catalase activity was increased (107 mg H₂O₂/100 g FW) indicating oxidative stress. Activity of catalase and peroxidase in control and acetylsalicylic acid treated plants were of similar increased values, probably due to the pathogen infection. In particular, peroxidase activity increased during tomato fructification. Alternative substitution of fungicides with aspirin may improve tomato quality and production.

References

1. Cavalcanti F. R., Vilela-Resende M. L., Lima J. P., Silveirac J. A., Oliveira J. T., Activities of antioxidant enzymes and photosynthetic responses in tomato pre-treated by plant activators and inoculated by Xanthomonas vesicatoria, Physiological and Molecular Plant Pathology, 2006, 68, 198-20810.1016/j.pmpp.2006.11.001
Search in Google Scholar
2. Chance B., Maehly A. C., Assay of catalase and peroxidases. Methods Enzymol, 1955, 2, 764-77510.1016/S0076-6879(55)02300-8
Search in Google Scholar
3. Cheruth A. J., Ksouri R., Ragupathi G., Paramasivam M., Jallali I., Hameed J., Zhao C. X., Shao H. B., Rajaram P., Antioxidant defense reponses: physiological plasticity in higher plants under abiotic constraints, Acta Physiol Plant, 2009, 31, 427 - 43610.1007/s11738-009-0275-6
Search in Google Scholar
4. Garcia P. C., Rivero R. M., Ruiz J. M., Romero L., The Role of Fungicides in the Physiology of Higher Plants: Implications for Defense Responses, The Botanical Review, 2003, 69 (2), 162-17210.1663/0006-8101(2003)069[0162:TROFIT]2.0.CO;2
Search in Google Scholar
5. Larkindale J., Bingru H., Effect of abscisic acid, salicilic acid, ethylene and hydrogen peroxid in thermotolerance and recovery for creeping bentgrass, Plant Growth Regulation, 2005, 47, 17 - 2810.1007/s10725-005-1536-z
Search in Google Scholar
6. Luck H., Peroxidase. In Methods of Enzymatic Analysis edited by Bergmeyer H.U., Academic Press, New York, 1963, 895-89710.1016/B978-0-12-395630-9.50159-6
Search in Google Scholar
7. Patykowski J., Role of hydrogen peroxide and apoplastic peroxidase in tomato - Botrytis cinerea interaction, Acta Physiologiae Plantarum, 2006, 28(6), 589-59810.1007/s11738-006-0054-6
Search in Google Scholar
8. Oancea S., Toxicologie alimentară si elemente de toxicologia mediului, edited by Oancea S., Ed. Universităţii “Lucian Blaga” din Sibiu, Sibiu, 2006
Search in Google Scholar
9. Savic S., Stikic R., Vucelic-Radovic B., Bogicevic B., Jovanovic Z., Hadz-Taskovic Sukalovic V., Comparative effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on growth and cell wall peroxidase activity in tomato fruits, Scientia Horticulturae, 2008, 117, 15 - 2010.1016/j.scienta.2008.03.009
Search in Google Scholar
10. Suciu Z., Plesca T., Goian M., Cultura legumelor în grădină, seră sisolar, edited by Suciu Z., Plesca T., Goian M., Ed. Facla, Timisoara, 1987
Search in Google Scholar
11. Zamfir A., Ecofiziologia plantelor : Îndrumător de lucrări practice, edited by Zamfir A., ULB Sibiu, 1993
Search in Google Scholar
12. *** www.astro-urseanu.ro/rasarit_soare_blank.html
Search in Google Scholar

Effects of Fungicide and Acetylsalicylic Acid Treatments on the Physiological and Enzymatic Activity in Tomato (Lycopersicon Esculentum Mill.)

Abstract

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