
Cannabidiol as a Water Additive: Impact on Performance, Egg Quality, Stress Reduction, and Gut Health in Laying Hens*
References
- Abraham A.D., Leung E.J.Y., Wong B.A., Rivera Z.M.G., Kruse L.C., Clark J.J., Land B.B. (2020). Orally consumed cannabinoids provide long-lasting relief of allodynia in a mouse model of chronic neuropathic pain. Neuropsychopharmacology, 45: 1105–1114.
- Aran A., Harel M., Cassuto H., Polyansky L., Schnapp A., Wattad N., Shmueli D., Golan D., Xavier Castellanos F. (2021). Cannabinoid treatment for autism: a proof-of-concept randomized trial. Mol. Autism, 12: 6.
- Atalay S., Jarocka-Karpowicz I., Skrzydlewska E. (2020). Antioxidative and anti-inflammatory properties of cannabidiol. Antioxidants (Basel), 9: 21.
- Balenović M., Janjecić Z., Savić V., Kas Please completeap A., Popović M., Šimpraga M., Sokolović M., Bedeković D., Kiš G., Zglavnik T., Špoljarić D,, Krstulović F., Listeš I., Zelenika T.A. (2024). Immunostimulatory and antibacterial effects of Cannabis sativa L. leaves on broilers. Animals (Basel), 14: 1159.
- Berkhoff J., Alvarado-Gilis C., Keim J.P., Alcalde J.A., Vargas-Bello-Perez E., Gandarillas M. (2020). Consumer preferences and sensory characteristics of eggs from family farms. Poult. Sci., 99: 6239–6246.
- Bień D., Michalczuk M., Jóźwik A., Matuszewski A., Konieczka P. (2024). Effects of Cannabis sativa extract on growth performance, meat physicochemical properties, and oxidative status in chickens challenged with Clostridium perfringens and lipopolysaccha-ride. Anim. Sci. Pap. Rep., 42: 81–108.
- Biernacki M., Brzóska M.M., Markowska A., Gałażyn-Sidorczuk M., Cylwik B., Gęgotek A., Skrzydlewska E. (2021). Oxidative stress and its consequences in the blood of rats irradiated with UV: protective effect of cannabidiol. Antioxidants (Basel), 10: 821.
- Blessing E.M., Steenkamp M.M., Manzanares J., Marmar C.R. (2015). Cannabidiol as a potential treatment for anxiety disorders. Neurotherapeutics, 12: 825–836.
- Bortolotti G.R., Negro J.J., Surai P.F., Prieto P. (2003). Carotenoids in eggs and plasma of red-legged partridges: effects of diet and reproductive output. Physiol. Biochem. Zool., 76: 367–374.
- Callaway J.C. (2004). Hempseed as a nutritional resource: An overview. Euphytica, 140: 65–72.
- Centraal Veevoederbureau (CVB) (2018). CVB Feed Table: Nutritional Values of Feed Materials for Poultry. Wageningen (NL): CVB.
- Costa B., Trovato A.E., Comelli F., Giagnoni G., Colleoni M. (2007). The non-psychoactive cannabis constituent cannabidiol is an orally effective therapeutic agent in rat chronic inflammatory and neuropathic pain. Eur. J. Pharmacol., 556: 75–83.
- David B., Mejdell C., Michel V., Lund V., Moe R.O. (2015). Air quality in alternative housing systems may have an impact on laying hen welfare. Part I – Ammonia. Animals, 5: 886–896.
- Deabold K.A., Schwark W.S., Wolf L., Wakshlag J.J. (2019). Single-dose pharmacokinetics and preliminary safety assessment with use of CBD-rich hemp nutraceutical in healthy dogs and cats. Animals, 9: 832.
- De Gregorio D., McLaughlin R.J., Posa L., Ochoa-Sanchez R., Enns J., Lopez-Canul M., Aboud M., Maione S., Comai S., Gobbi G. (2019). Cannabidiol modulates serotonergic transmission and reverses both allodynia and anxiety-like behavior in a model of neuropathic pain. Pain, 160: 136–150.
- Della Rocca G., Di Salvo A. (2020). Hemp in veterinary medicine: from feed to drug. Front. Vet. Sci., 7: 387.
- de Mello Schier A.R., de Oliveira Ribeiro N.P., Coutinho D.S., Machado S., Arias-Carrión O., Crippa J.A., Zuardi A.W., Nardi A.E., Silva A.C. (2014). Antidepressant-like and anxiolytic-like effects of cannabidiol: a chemical compound of Cannabis sativa. CNS Neurol. Disord. Drug Targets, 13: 953–960.
- Derkho M.A., Sajfutdinova L.N., Strizhikov V.K., Strizhikova S.V., Ponomaryova T.A. (2021). The role of corticosterone in the regulation of the cellular composition of chicken blood during the stress reaction. E3S Web Conf., 282: 03003.
- Devinsky O., Cross J.H., Laux L., Marsh E., Miller I., Nabbout R., M.D., Scheffer I.E., Thiele E.A., Wright S. (2017). Trial of cannabidiol for drug-resistant seizures in the Dravet syndrome. N. Engl. J. Med., 376: 2011–2020.
- EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP). (2011). Scientific opinion on the safety of hemp (Cannabis genus) for use as animal feed. EFSA J., 9: 2011.
- Elphick M.R., Egertova M. (2001). The neurobiology and evolution of cannabinoid signalling. Philos. Trans. R. Soc. Lond. B Biol. Sci., 356: 381–408.
- El-Sabrout K., Ahmad S., Buonaiuto G. (2025). Phytogenics, fermented ingredients, bee products, insect additives, and byproducts as promising dietary supplements for poultry. Ann. Anim. Sci., 26: 253–276.
https://doi.org/10.2478/aoas-2025-0049 - European Commission (2025). Market situation for eggs, 22 May 2025. Available online:
https://agriculture.ec.europa.eu/farming/animal-products/eggs_en . Accessed 30 May 2025. - European Union (2010). Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. Available from:
https://eur-lex.europa.eu/legal-content/EN/TXT/HTML/?uri=CELEX:32010L0063 . Accessed February 2023 - Ferreira J.A., Geraldo A., KaiqueValentim J., Silva E.A., Miranda D.A., Lemke S.S.R., de Almeida A.A., Mendes J.P. (2022). Emulsifier inclusion in diets with energy reduction for laying hens. Cuban J. Agric. Sci., 56: 1–9.
- Fluck A.C., Pires P.G.S., Cardinal K.M., Costa O.A.D., Ávila B.P., Rosa P.P., Medeiros L.A., Borba L.P. (2023). Yolk and eggshell: are these the parameters that influence eggs purchasing? Obs. Econ. Latinoam., 21: 6130–6151.
- Gakhar N., Goldberg E., Jing M., Gibson R., House J.D. (2012). Effect of feeding hemp seed and hemp seed oil on laying hen performance and egg yolk fatty acid content: evidence of their safety and efficacy for laying hen diets. Poult. Sci., 91: 701–711.
- Galanakis C. (2017). What is the difference between bioavailability, bioaccessibility and bioactivity of food components. SciTech Connect. Available from:
http://scitechconnect.elsevier.com/bioavailability-bioaccessibilitybioactivity-food-components/ - Gamble L.J., Boesch J.M., Frye C.W., Schwark W.S., Mann S., Wolfe L., Brown H., Berthelsen E.S., Wakshlag J.J. (2018). Pharmacokinetics, safety, and clinical efficacy of cannabidiol treatment in osteoarthritic dogs. Front. Vet. Sci., 5: 165.
- Granstad S., Kristoffersen A.B., Benestad S.L., Sjurseth S.K., David B., Sørensen L., Fjermedal A., Edvardsen D.H., Sanson G., Løvland A., Kaldhusdal M. (2020). Effect of feed additives as alternatives to in-feed antimicrobials on production performance and intestinal Clostridium perfringens counts in broiler chickens. Animals, 10: 240.
- Hammell D.C., Zhang L.P., Ma F., Abshire S.M., McIlwrath S.L., Stinchcomb A.L., Westlund K.N. (2016). Transdermal cannabidiol reduces inflammation and pain related behaviours in a rat model of arthritis. Eur. J. Pain, 20: 936–948.
- Hudon J. (1994). Biotechnological applications of research on animal pigmentation. Biotechnol. Adv., 12: 49–69.
- Hunt A.B.G., Flint H.E., Logan D.W., King T. (2023). A single dose of cannabidiol (CBD) positively influences measures of stress in dogs during separation and car travel. Front. Vet. Sci., 10: 1112604.
- Hussain T., Jeena G., Pitakbut T., Vasilev N., Kayser O. (2021). Cannabis sativa research trends, challenges, and new-age perspectives. iScience, 24: 103391.
- Janicka K., Drabik K., Wengerska K., Rozempolska-Rucińska I. (2025). Effect of stocking density on behavioural and physiological traits of laying hens. Animals, 15: 604.
- Jing M., Zhao S., House J.D. (2017). Performance and tissue fatty acid profile of broiler chickens and laying hens fed hemp oil and HempOmegaTM. Poult. Sci., 96: 1809–1819.
- Kanbur G., Göçmen R., Cufadar Y. (2023). A comparative study on the effects of hemp seed oil versus four different UFA-rich seed oils dietary supplementation on egg production performance, egg quality, and yolk fatty acids in laying hens. Trop. Anim. Health Prod., 55: 6.
- Kasula R., Solis F., Shaffer B., Connett F., Barret C., Cocker R., Willinghan E. (2021). Effect of increasing levels of dietary hemp seed cake on egg quality in commercial laying hens. Int. J. Poult. Sci., 20: 48–58.
- Khan R.U., Durrani F.R., Chand N., Anwar H. (2010). Influence of feed supplementation with Cannabis sativa on quality of broilers carcass. Pak. Vet. J., 30: 34–38.
- Kim D.H., Lee Y.K., Lee S.D., Kim S.H., Lee S.R., Lee H.G., Lee K.W. (2020). Changes in production parameters, egg qualities, fecal volatile fatty acids, nutrient digestibility, and plasma parameters in laying hens exposed to ambient temperature. Front. Vet. Sci., 7: 412.
- Kim H.R., Ryu C., Lee S.D., Cho J.H., Kang H. (2024). Effect of heat stress on the laying performance, egg quality, and physiological response of laying hens. Animals, 14: 1076.
- Konca Y., Cimen B., Yalcin H., Kaliber M., Beyzi S.B. (2014). Effect of hempseed (Cannabis sativa sp.) inclusion to the diet on performance, carcass and antioxidative activity in Japanese quail (Coturnix coturnix japonica). Korean J. Food Sci. Anim. Res., 34: 141–150.
- Konieczka P., Szkopek D., Kinsner M., Fotschki B., Juśkiewicz J., Banach J. (2020). Cannabis-derived cannabidiol and nanoselenium improve gut barrier function and affect bacterial enzyme activity in chickens subjected to C. perfringens challenge. Vet. Res., 51: 141.
- Kumar A., Premoli M., Aria F., Bonini S.A., Maccarinelli G., Gianoncelli A., Memo M., Mastinu A. (2019). Cannabimimetic plants: are they new cannabinoidergic modulators? Planta, 249: 1681–1694.
- Laux L.C., Bebin E.M., Checketts D., Chez M., Flamini R., Marsh E.D., Miller I., Nichol K., Park Y., Segal E. (2019). Long-term safety and efficacy of cannabidiol in children and adults with treatment-resistant Lennox-Gastaut syndrome or Dravet syndrome: Expanded access program results. Epilepsy Res., 154: 13–20.
- Lehmann C., Fisher N.B., Tugwell B., Szczesniak A., Kelly M., Zhou J. (2016). Experimental cannabidiol treatment reduces early pancreatic inflammation in type 1 diabetes. Clin. Hemorheol. Microcirc., 64: 655–662.
- Li H., Kong W., Chambers C.R., Yu D., Ganea D., Tuma R.F., Ward S.J. (2018). The nonpsychoactive phytocannabinoid cannabidiol (CBD) attenuates pro-inflammatory mediators, T cell infiltration, and thermal sensitivity following spinal cord injury in mice. Cell. Immunol., 329: 16.
- Lohmann (2020). Lohmann Brown Classic layers management guide cage housing. Available from:
https://lohmann-breeders.com/media/strains/cage/management/Classic-Cage.pdf . Accessed May 2023 - Mahmoudi M., Farhoomand P., Nourmohammadi R. (2015). Effects of different levels of hempseed (Cannabis sativa L.) and dextran oligosaccharide on growth performance and antibody titer response of broiler chickens. Ital. J. Anim. Sci., 14: 3473.
- Maione S., Piscitelli F., Gatta L., Vita D., De Petrocellis L., Palazzo E., De Novellis V., Di Marzo V. (2011). Non-psychoactive cannabinoids modulate the descending pathway of antinociception in anaesthetized rats through several mechanisms of action. Br. J. Pharmacol., 162: 584–596.
- Mendes L.J., Moura M.A., Maciel M.P., Reis S.T., Silva V.G., Silva D.B., Santana de Moura V.H., Alves I.M., Sampaio Said J.L. (2017). Perfil do consumidor de ovos e carne de frango do município de Janaúba-MG. ARS Veterinaria, 32: 81–87.
- Millar S.A., Stone N.L., Yates A.S., O'Sullivan S.E. (2018). A systematic review on the pharmacokinetics of cannabidiol in humans. Front. Pharmacol., 9: 1365.
- Mohamed A., Khalil M., Soliman F., El-Sabrout K. (2025). The effect of drinking ionized water on the productive performance, physiological status, and carcass characteristics of broiler chicks. Animals, 15: 229.
- Mortensen A. (2006). Carotenoids and other pigments as natural colorants. Pure Appl. Chem., 78: 1477–1491.
- Mozaffari K., Willette S., Lucker B.F., Kovar S.E., Holguin F.O., Guzman I. (2021). The effects of food on cannabidiol bioaccessibility. Molecules, 26: 3573.
- Nazar F.N., Magnoli A.P., Dalcero A.M., Marin R.H. (2012). Effect of feed contamination with aflatoxin B1 and administration of exogenous corticosterone on Japanese quail biochemical and immunological parameters. Poult. Sci., 91: 47–54.
- Nys Y. (2000). Dietary carotenoids and egg yolk coloration – a review. Arch. Geflugelkd., 64: 45–54.
- Oltrabella F., Melgoza A., Nguyen B., Guo S. (2017). Role of the endocannabinoid system in vertebrates: emphasis on the zebrafish model. Dev. Growth Differ., 59: 194–210.
- Pertwee R.G. (2008). The diverse CB1 and CB2 receptor pharmacology of three plant cannabinoids: delta9-tetrahydrocannabinol, cannabidiol and delta9-tetrahydrocannabivarin. Br. J. Pharmacol., 153: 199–215.
- Pirgozliev V., Rose S.P., Ivanova S. (2019). Feed additives in poultry nutrition. Bulg. J. Agric. Sci., 25: 8–11.
- R Core Team (2024). R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. Available from:
https://www.R-project.org/ - Rosso E., Armone R., Costale A., Meineri G., Chiofalo B. (2024). Hemp seed (Cannabis sativa L.) varieties: Lipids profile and anti-oxidant capacity for monogastric nutrition. Animals, 14: 2699.
- Saleh A.A., Gawish E., Mahmoud S.F., Amber K., Awad W., Alzawqari M.H., Shukry M., Abdel-Moneim A.-M.E. (2021). Effect of natural and chemical colorant supplementation on performance, egg quality characteristics, yolk fatty-acid profile, and blood constituents in laying hens. Sustainability, 13: 4503.
- Salzet M., Stefano G.B. (2002). The endocannabinoid system in invertebrates. Prostaglandins Leukot. Essent. Fatty Acids, 66: 353–361.
- Samara E., Bialer M., Mechoulam R. (1988). Pharmacokinetics of cannabidiol in dogs. Drug Metab. Dispos., 16: 469–472.
- Sana B., Chand N., Naz S., Alhidary I.A., Khan R.U., Shamsi S., Losacco C., Tufarelli V. (2024). Effects of hempseed (Cannabis sativa L.) in diet on growth, gut health, and immunity in broilers. Vet. Q., 44: 1–10.
- Seuss-Baum I., Nau F., Guérin-Dubiard C. (2011). The nutritional quality of eggs. In: Improving the safety and quality of eggs and egg products. Woodhead Publishing, pp. 201–236.
- Silversides F.G., Lefrançois M.R. (2005). The effect of feeding hemp seed meal to laying hens. Br. Poult. Sci., 46: 231–235.
- Skinner C.M., Nookaew I., Ewing L.E., Wongsurawat T., Jenjaroenpun P., Quick C.M., Yee E.U., Piccolo B.D., ElSohly M., Walker L.A., Gurley B., Koturbash I. (2020). Potential probiotic or trigger of gut inflammation – the Janus-faced nature of cannabidiol-rich cannabis extract. J. Diet. Suppl., 17: 543–560.
- Skřivan M., Englmaierová M., Vít T., Skřivanová E. (2019). Hempseed increases gamma-tocopherol in egg yolks and the breaking strength of tibias in laying hens. PLoS One, 14: e0217509.
- Sopian Y., Sartsook A., Arjin C., Lumsangkul C., Sringarm K., Sivapirunthep P., Chaosap C. (2024). Dietary supplementation of Cannabis sativa residues in broiler chickens affects performance, carcass characteristics, intestinal morphology, blood biochemistry profile and oxidative stability. Poult. Sci., 103: 104117.
- Souza R.P. de P., Laurentiz A.C. de, Faria G.A., Filardi R. da S., Mello E. do S. (2019). Use of soybean gum as an emulsifier in diets for commercial laying hens. Pesqui. Agropecu. Bras., 54: e00683.
- Stanciu C.N., Brunette M.F., Teja N., Budney A.J. (2021). Evidence for use of cannabinoids in mood disorders, anxiety disorders, and PTSD: a systematic review. Psychiatr. Serv., 72: 429–436.
- Stith S.S., Li X., Diviant J.P., Brockelman F.C., Keeling K.S., Hall B., Vigil J.M. (2020). The effectiveness of inhaled Cannabis flower for the treatment of agitation/irritability, anxiety, and common stress. J. Cannabis Res., 2: 47.
- Suraev A.S., Marshall N.S., Vandrey R., McCartney D., Benson M.J., McGregor I.S., Grunstein R.R., Hayas C.M. (2020). Cannabinoid therapies in the management of sleep disorders: a systematic review of preclinical and clinical studies. Sleep Med. Rev., 53: 101339.
- Surai P., Speake B., Sparks N. (2001). Carotenoids in avian nutrition and embryonic development. 1. Absorption, availability and levels in plasma and egg yolk. J. Poult. Sci., 38: 1–27.
- Szaflarski J.P., Bebin E.M., Cutter G., DeWolfe J., Dure L.S., Gaston T.E., Kankirawatana P., Liu Y., Singh R., Standaert D.G. (2018). Cannabidiol improves frequency and severity of seizures and reduces adverse events in an open-label add-on prospective study. Epilepsy Behav., 87: 131–136.
- Tufarelli V., Losacco C., Tedone L., Passantino L., Tarricone S., Laudadio V., Colonna M.A. (2023). Hemp seed (Cannabis sativa L.) cake as sustainable dietary additive in slow growing broilers: effects on performance, meat quality, oxidative stability and gut health. Vet. Q., 43: 1–12.
- Valizadehderakhshan M., Shahbazi A., Kazem-Rostami M., Todd M.S., Bhowmik A., Wang L. (2021). Extraction of cannabinoids from Cannabis sativa L. (hemp). Agriculture, 11: 384.
- Verrico C.D., Wesson S., Konduri V., Hofferek C.J., Vazquez-Perez J., Blair E., Dunner K. Jr., Salimpour P., Decker W.K., Halpert M.M. (2020). A randomized, double-blind, placebo-controlled study of daily cannabidiol for the treatment of canine osteoarthritis pain. Pain, 161: 2191–2202.
- Villanueva M.R.B., Joshaghani N., Villa N., Badla O., Goit R., Saddik S.E., Dawood S.N., Rabih A.M., Niaj A., Raman A., Uprety M., Calero M., Khan S. (2022). Efficacy, safety, and regulation of cannabidiol on chronic pain: a systematic review. Cureus, 14: e26913.
- Xu Y., Lai X., Li Z., Zhang X., Luo Q. (2018). Effect of chronic heat stress on some physiological and immunological parameters in different breed of broilers. Poult. Sci., 97: 4073–4082.
- Zurak D., Svečnjak Z., Kiš G., Janječić Z., Bedeković D., Duvnjak M., Pirgozliev V., Grbeša D., Kljak K. (2024). Carotenoid deposition in yolks of laying hens fed with corn diets differing in grain hardness and supplemented with rapeseed oil and emulsifier. Poult. Sci., 103: 103922.
DOI: https://doi.org/10.2478/aoas-2026-0016 | Journal eISSN: 2300-8733 (formerly 1642-3402) | Journal ISSN: 1642-3402
Language: English
Submitted on: Jun 27, 2025
Accepted on: Feb 6, 2026
Published on: Oct 6, 2026
Published by: National Research Institute of Animal Production
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
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© 2026 Sepideh Fallahi, Łukasz Bobak, Anna Lipińska, Muhammad Umair Asghar, Katarzyna Olejnik, Sebastian Opaliński, published by National Research Institute of Animal Production
This work is licensed under the Creative Commons Attribution 3.0 License.