The effects of defatted black soldier fly larvae meals (ECBSFL and OSBSFL) as fish meal alternatives in the diet of Penaeus vannamei (Boone, 1931)

T. Bhuvaneshwaran; Narottam Prasad Sahu; Naseemashahul Shamna; Parimal Sardar; Muralidhar P. Ande; Karthireddy Syamala; Shivali Sugund; Siddharth Sharma; Nisha Chuphal; N. Raghuvaran; Samrat Kumar Nirala

doi:10.2478/aoas-2025-0133

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The effects of defatted black soldier fly larvae meals (ECBSFL and OSBSFL) as fish meal alternatives in the diet of Penaeus vannamei (Boone, 1931)

Annals of Animal Science

AHEAD OF PRINT

By: T. Bhuvaneshwaran, Narottam Prasad Sahu, Naseemashahul Shamna, Parimal Sardar, Muralidhar P. Ande, Karthireddy Syamala, Shivali Sugund, Siddharth Sharma, Nisha Chuphal, N. Raghuvaran and Samrat Kumar Nirala

Open Access

|Jun 2026

Abstract

A 60-day feeding trial evaluated the effects of defatted black soldier fly larvae (BSFL) (Hermetia illucens) raised in environment controlled or open systems as fish meal (FM) replacers on whiteleg shrimp (Penaeus vannamei) juveniles in brackish water (9 ppt). Nine experimental diets (36% crude protein, 372.49 Kcal digestible energy/100 g feed) replaced the FM with graded levels of defatted meals of BSFL raised in an environment controlled system (ECBSFL) or an open system (OSBSFL) at 0% (control), 25%, 50%, 75%, or 100%. 1,080 juveniles (average. weight 2.0±0.25 g) were distributed into nine groups (triplicates, 40 shrimp per hapa) and fed four times daily. Shrimps fed on the ECBSFL_75% diet had the best final body weight (18.43±0.11 g), weight gain percentage (773.22±8.21), specific growth rate (3.61±0.017%/day) and feed conversion ratio (1.36±0.01) and no significant changes in whole-body composition and survival rate. Protease activity (units/min/mg protein) was higher in the ECBSFL_50% (3.11±0.19) and ECBSFL_75% (3.61±0.15) groups, while the control group had higher lipase activity (0.46±0.02 units/hour/mg protein). The ECBSFL_75% group showed higher hepatopancreatic AST (5.11±0.24) and ALT (2.99±0.28) activities (units/min/mg protein) and serum total protein (5.65±0.70 g/dl), while the OSBSFL_100% group had higher serum glucose (81.07±3.36 mg/dl) but lower cholesterol (87.23±2.26 mg/dl) and triglycerides (128.51±0.65 mg/dl). Based on these results, it can be concluded that up to 75% of fish meal can be replaced with meal of BSFL raised in an environment controlled system at the inclusion level of 18.75%, or up to 50% with meal of BSFL raised in an open system at the inclusion level of 12.50% without adverse effects on the growth, nutrient utilization, physio-metabolic status, and immune responses of Penaeus vannamei reared in brackish water.

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Articles in this issue

DOI: https://doi.org/10.2478/aoas-2025-0133 | Journal eISSN: 2300-8733 | Journal ISSN: 1642-3402

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Language: English

Submitted on: Sep 3, 2025

Accepted on: Nov 26, 2025

Published on: Jun 6, 2026

Published by: National Research Institute of Animal Production

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

growth performance,

insect meal,

immune response,

physio-metabolic status,

shrimp feed

Related subjects:

Medicine,

Veterinary medicine

© 2026 T. Bhuvaneshwaran, Narottam Prasad Sahu, Naseemashahul Shamna, Parimal Sardar, Muralidhar P. Ande, Karthireddy Syamala, Shivali Sugund, Siddharth Sharma, Nisha Chuphal, N. Raghuvaran, Samrat Kumar Nirala, published by National Research Institute of Animal Production
This work is licensed under the Creative Commons Attribution 4.0 License.

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