References
- Adineh H., Naderi M., Khademi Hamidi M., Harsij M. (2019) Biofloc technology improves growth, innate immune responses, oxidative status, and resistance to acute stress in common carp (Cyprinus carpio) under high stocking density. Fish Shellfish Immunol., 95: 440-448.
- Ahmed I., Leya T., Saharan N., Majeedkutty B.R.A., Rathore G., Gora A.H., Bhat I.A., Verma A.K. (2019). Carbon sources affects water quality and haemato-biochemical responses of Labeo rohita in zero water exchange biofloc system. Aquac. Res., 50: 2879–2887.
- Amparyup P., Charoensapsri W., Tassanakajon A. (2009). Two prophenoloxidases are important for the survival of Vibrio harveyi challenged shrimp Penaeus monodon. Dev. Comp. Immunol., 33: 247-256.
- Amriawati E., Budiardi T., Setiawati M., Rohmana D., Ekasari J.( 2021). Digestive system and growth performance of giant gourami (Osphronemus goramy Lacepede) juveniles in biofloc systems fed with different feed types. Aquac. Res., 52: 4661–4669.
- Arambul-Muñoz E., Ponce-Palafox J.T., Santos R.C.D., Aragón-Noriega E.A., Rodríguez-Domínguez G., Castillo-Vargasmachuca S.G. (2019). Infuence of stocking density on production and water quality of a photoheterotrophic intensive system of white shrimp (Penaeus vannamei) in circular lined grow-out ponds, with minimal water replacement. Lat. Am. J. Aquat. Res., 47: 449–455.
- Araneda M., Pérez E.P., Gasca-Leyva E. (2008). White shrimp Penaeus vannamei culture in freshwater at three densities: condition state based on length and weight. Aquaculture, 283: 13–18.
- Avnimelech Y. (2015). Biofloc Technology–A Practical Guide Book, 3rd ed. The World Aquaculture Society, Baton Rouge, LA, USA.
- Bajracharya S., Roy L.A., García J.C., Davis D.A. (2025). Stocking density and growth of Pacific white shrimp Litopenaeus vannamei in intensive recirculating (indoor biofloc and outdoor mixotrophic) systems. N. Am. J. Aquac., 87: 28-36.
- Bardera G., Usman N., Owen M., Pountney D., Sloman K.A., Alexander M.E. (2019). Theimportance of behaviour in improving the production of shrimp in aquaculture. Rev. Aquac., 11: 1104–1132.
- Bateman K.S. (2021). Viruses affecting crustaceans. P: 305–340. In: Hurst C.J. (Ed.). Studies in Viral Ecology. John Wiley and Sons, USA.
- Becerra-Dórame M.J., Martínez-Porchas M., Martínez-Córdova L.R., Rivas-Vega M.E., Lopez-Elias J.A., Porchas-Cornejo M.A. (2012). Production response and digestive enzymatic activity of the Pacifc white shrimp Litopenaeus vannamei (Boone, 1931) intensively pregrown in microbial heterotrophic and autotrophic-based systems. Sci. World J., Article ID 723654, 6 pages.
- Behera S., Das P.C., Felix N., Ferosekhan S., Swain H.S., Kumari R., Athithan S., Padmavathy P. (2025). Effect of different carbon supplements on growth performance and digestive enzyme activities of butter catfish (Ompok bimaculatus Bloch, 1794) in biofloc system. Aquaculture, 603: 742384.
- Dong J., Zhao Y.Y., Yu Y.H., Sun N., Li Y.D., Wei H., Yang Z.Q., Li X.D., Li L. (2018) Effect of stocking density on growth performance, digestive enzyme activities, and nonspecific immune parameters of Palaemonetes sinensis. Fish Shellfish Immunol., 73: 37-41.
- Ellis A. E. (1990). Lysozyme Assays. Techniques in Fish Immunology, Pp.101-103. Emerenciano M.G.C., Khanjani M.H., Sharifinia M., Miranda-Baeza A. (2025). Could Biofloc Technology (BFT) Pave the Way Toward a More Sustainable Aquaculture in Line With the Circular Economy?. Aquac. Rese., 2025: 1020045, 23 pages
- Fagutao F.F., Koyama T., Kaizu A., Saito-Taki T., Kondo H., Aoki T., Hirono I. (2009). Increased bacterial load in shrimp hemolymph in the absence of prophenoloxidase. FEBS Journal, 276: 5298-5306.
- Fan T., Jing Z., Fan X., Yu M., Jiang G. (2011). Purification and characterization of phenoloxidase from brine shrimp Artemia sinica. Acta Biochim. Biophys. Sin., 43: 722–728.
- Fleckenstein L.J., Kring N.A., Tierney T.W., Fisk J.C., Lawson B.C., Ray A.J. (2020). The effects of artifcial substrate and stocking density on Pacifc white shrimp (Litopenaeus vannamei) performance and water quality dynamics in high tunnel-based biofoc systems. Aquac. Eng., 90: 102093.
- Ghosh A.K., Sarkar S., Bir J., Islam S.S., Huq K.A., Naser M.N. (2013). Probiotic tiger shrimp (Penaeus monodon) farming at different stocking densities and its impact on production and economics. Int. J. Res. Fish. Aquac., 3: 25–29.
- Guemez-Sorhouet E., Villarreal H., Racotta I.S., Naranjo J., Mercier L. (2019). Zootechnical and physiological responses of whiteleg shrimp (Litopenaeus vannamei) postlarvae reared in bioflocs and subjected to stress conditions during nursery phase. Aquac. Res., 50: 1198–1211.
- Gyan W.R., Yang Q., Tan B., Jan S.S., Jiang L., Chi S., Dong X., Liu H., Shuang Z. (2020). Effects of antimicrobial peptides on growth, feed utilization, serum biochemical indices and disease resistance of juvenile shrimp, Litopenaeus vannamei. Aquac. Res., 51: 1222–1231.
- Hussain A.S., Mohammad D.A., Sallam W.S., Shoukry N.M., Davis D.A. (2021). Effects of culturing the Pacific white shrimp Penaeus vannamei in biofloc vs synbiotic systems on the growth and immune system. Aquaculture, 542: 736905.
- Irani M., Islami H. R., Bahabadi M. N., Shekarabi S.P.H. (2023). Production of Pacific white shrimp under different stocking density in a zero-water exchange biofloc system: Effects on water quality, zootechnical performance, and body composition. Aquac. Eng., 100: 102313.
- Jones C.M., Ng W.K., King M. (2010). Alsever’s solution: a review of its history, chemistry, and production. Transfus. Med. Rev., 24: 259–267.
- Kaya D. (2025). Improvement of brown shrimp (Penaeus aztecus) culture parameters through dietary enriched synbiotic in a biofloc system. Aquac. Int., 33: 232.
- Khanjani M.H., Alizadeh M. (2024). Effects of different salinity levels on performance of Nile tilapia fingerlings in a biofloc culture system. Ann. Anim. Sci., 24: 235–245.
- Khanjani M.H., Eslami J., Emerenciano M.G.C. (2025b). Wheat flour as carbon source on water quality, growth performance, hemolymph biochemical and immune parameters of Pacific white shrimp (Penaeus vannamei) juveniles in biofloc technology (BFT). Aquac. Rep., 40: 102623,
- Khanjani M.H., Eslami J., Ghaedi G., Sourinejad I. (2022b) The effects of different stocking densities on nursery performance of banana shrimp (Fenneropenaeus merguiensis) reared under biofloc condition. Ann. Anim. Sci., 22: 1291–1299.
- Khanjani M.H., Mohammadi A. (2025). Microplastic pollution in aquatic ecosystems: A comprehensive review of impacts on aquatic animals. Ann. Anim. Sci., DOI: 10.2478/aoas-2025-0051.
- Khanjani M.H., Mohammadi A., Sharifinia M., Singh S.K. (2025a). Assessing the impact of microplastics and nanoplastics on shrimp growth, physiology, antioxidant, immune responses and gut microbiota. Mar. Freshw. Res., 76: MF25008.
- Khanjani M.H., Mozanzadeh M.T., Gisbert E., Hoseinifar S.H. (2024a). Probiotics, prebiotics, and synbiotics in shrimp aquaculture: Their effects on growth performance, immune responses, and gut microbiome. Aquac. Rep., 38: 102362.
- Khanjani M.H., Sharifinia M. (2022). Biofloc as a food source for Banana shrimp (Fenneropenaeus merguiensis) postlarvae. N. Am. J. Aquac., 45: 469-479.
- Khanjani M.H., Sharifinia M., Akhavan-Bahabadi M., Emerenciano M.G.C. (2024b). Probiotics and phytobiotics as dietary and water supplements in biofloc aquaculture systems. Aquac. Nut., Article ID 3089887.
- Khanjani M.H., Torfi Mozanzade M., Fóes G.K. (2022a). Aquamimicry system: a sutiable strategy for shrimp aquaculture. Ann. Anim. Sci., 22: 1201-1210.
- Khanjani MH., Zahedi S., Sharifinia M., Hajirezaee S., Singh S.K. (2025c). Biological removal of nitrogenous waste compounds in the biofloc aquaculture system: a review. Ann. Anim. Sci., 25: 3-21.
- Kim S.K., Pang Z., Seo H.C., Cho Y.R., Samocha T., Jang I.K. (2014). Effect of bioflocs on growth and immune activity of Pacific white shrimp, Litopenaeus vannamei postlarvae. Aquac. Res., 45: 362–371.
- Kumar S., Anand P.S.S., De D., Deo A.D., Ghoshal T.K., Sundaray J.K., Ponniah A.G., Jithendran K.P., Raja R.A., Biswas G., Lalitha N. (2017). Effects of biofloc under different carbon sources and protein levels on water quality, growth performance and immune responses in black tiger shrimp Penaeus monodon (Fabricius, 1978). Aquac. Res., 48: 1168–1182.
- Li X., Dong S., Lei Y., Li Y. (2007) The effect of stocking density of Chinese mitten crab Eriocheir sinensis on rice and crab seed yields in rice–crab culture systems. Aquaculture, 273: 487-493.
- Lin Y.C., Chen J.C., Chen Y.Y., Yeh S.T., Chen L.L., Huang C.L., Li C.C. (2015). Crowding of white shrimp Litopenaeus vananmei depresses their immunity to and resistance against Vibrio alginolyticus and white spot syndrome virus. Fish. Shellfish Immunol., 45: 104–111.
- Liu G., Zhu S., Liu D., Guo X., Ye Z. (2017). Effects of stocking density of the white shrimp Litopenaeus vannamei (Boone) on immunities, antioxidant status, and resistance against Vibrio harveyi in a biofloc system. Fish. Shellfish Immunol., 67: 19-26.
- Liu T., Zhang G. Feng Y., Kong C., Ayisi C.L., Huang X., Hua X. (2019). Dietary soybean antigen impairs growth and health through stress-induced non-specific immune responses in Pacific white shrimp, Litopenaeus vannamei. Fish. Shellfish Immunol., 84: 124-129
- Luedemana R.A., Lightnera D.V. (1992). Development of an in vitro primary cell culture system from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei. Aquaculture, 101: 205–211.
- Martinez-Porchas M., Ezquerra-Brauer M., Mendoza-Cano F., Higuera J.E.C., Vargas-Albores F., Martinez-Cordova L.R. (2020). Effect of supplementing heterotrophic and photoautotrophic biofloc, on the production response, physiological condition and post-harvest quality of the whiteleg shrimp, Litopenaeus vannamei. Aquac. Rep., 16: 100257.
- Mendez C.A., Morales M.C., Brokordt K. (2024). Effects of stocking density of the river shrimp Cryphiops caementarius on physiological and performance responses in a biofloc system. Fishes, 9: 377.
- Mercier L., Palacios E., Campa -Córdova A., Tovar -Ramírez D., Hernández - Herrera R., Racotta I. (2006). Metabolic and immune responses in Pacific whiteleg shrimp Litopenaeus vannamei exposed to a repeated handling stress. Aquaculture, 258: 633 -640.
- Minabi K., Sourinejad I., Alizadeh M., Ghatrami E.R., Khanjani M.H. (2020). Effects of different carbon to nitrogen ratios in the biofloc system on water quality, growth, and body composition of common carp (Cyprinus carpio L.) fingerlings. Aquac. Int., 28: 1883–1898.
- Nayak B., Kumar S., Collins P. L., Samal S. K. (2008). Molecular characterization and complete genome sequence of avian paramyxovirus type 4 prototype strain duck/Hong Kong/D3/75. Virol. J., 5: 124
- Negrini C., de Castro C.S., Bittencourt-Guimarães A.T., Frozza A., Ortiz-Kracizy R., Cupertino-Ballester E.L. (2017). Stocking density for freshwater prawn Macrobrachium rosenbergii (Decapoda, Palaemonidae) in biofloc system. Lat. Am. J. Aquat. Res., 45(5): 891-899.
- Nguyen N.H.Y., Trinh L.T., Chau D.T, Baruah K., Lundh T., Kiessling A. (2019). Spent brewer’s yeast as a replacement for fshmeal in diets for giant freshwater prawn (Macrobrachium rosenbergii), reared in either clear water or a biofoc environment. Aquac. Nutr. 25: 970–979.
- Parrilla-Taylor D.P., Zenteno-Savín T. (2011). Antioxidant enzyme activities in Pacific white shrimp (Litopenaeus vannamei) in response to environmental hypoxia and reoxygenation, Aquaculture, 318: 379-383.
- Prangnell D.I., Castro L.F., Ali A.S., Browdy C.L., Zimba P.V., Laramore S.E., Samocha T.M. (2016). Some limiting factors in super-intensive production of juvenile Pacific white shrimp, Litopenaeus vannamei, in no water exchange, biofloc-dominated systems. J. World Aquac. Soc., 47: 396–413.
- Promthale P., Pongtippatee P., Withyachumnarnkul B., Wongpraserta K. (2019). Bioflocs substituted fishmeal feed stimulates immune response and protects shrimp from Vibrio parahaemolyticus infection. Fish Shellfish Immunol., 93: 1067–1075.
- Rodríguez-Olague D., Ponce-Palafox J.T., Castillo-Vargasmachuca S.G., Arambul-Munoz E., de los Santos R.C., Esparza-Leal H.M. (2021). Effect of nursery system and stocking density to produce juveniles of whiteleg shrimp Litopenaeus vannamei. Aquac. Rep., 20: 100709.
- Said M.M., Abo-Al-Ela H., El-Barbary Y.A., Ahmed O.M., Dighiesh H.S. (2024). Infuence of stocking density on the growth, immune and physiological responses, and cultivation environment of white-leg shrimp (Litopenaeus vannamei) in biofoc systems, Sci. Rep., 14: 11147.
- Said M.M., El-barbary Y.A., Ahmed O. M. (2022). Assessment of performance, microbial community, bacterial food quality, and gene expression of whiteleg shrimp (Litopenaeus vannamei) reared under different density biofloc systems. Aquac. Nut., 2022: 3499061, 13 pages.
- Samocha T.M., Prangnell D.I., Castro L.F., Zeigler T.R., Advent B. (2015). Nursery performance of Pacific White Shrimp in zero-exchange biofloc systems. Glob. Aquac. Advocate, 26–28 (January/February).
- Samocha T.M., Prangnell D.I., Hanson T.R., Treece G.D., Morris T.C., Castro L.F., Staresinic N. (2017). Design and operation of super intensive, biofloc-dominated systems for indoor production of the Pacific White Shrimp, Litopenaeus vannamei – The Texas A&M AgriLife Research Experience. The World Aquaculture Society, Baton Rouge, LA, USA.
- Santos F.A.C., da Costa Julio G.S., Luz R.K. (2021). Stocking density in Colossoma macropomum larviculture a freshwater fsh in recirculating aquaculture system. Aquac. Res., 52: 1185–1191.
- Shailesh S., Sahoo P.K. (2008). Lysozyme: an important defence molecule of fish innate immune system. Aquac. Res., 39 (3): 223-239.
- Shan H., Geng Z., Ma S., Wang T. (2019). Comparative study of the key enzymes and biochemical substances involved in the energy metabolism of Pacific white shrimp, Litopenaeus vannamei, with different ammonia-N tolerances. Compar. Biochem. Physiol. C 221: 73–81.
- Söderhäll K., Hall L. (1984). Lipopolysaccharide-induced activation of prophenoloxidase activating system in crayfish haemocyte lysate. BBA-Bioenergetics, 797: 99-104.
- Tao C.T., Hai T.N., Terahara T., Hoa N.V. (2021). Influence of stocking density on survival and growth of larval and postlarval white leg shrimp (Litopenaeus vannamei Boone, 1931) applied biofloc technology. AACL Bioflux, 14: 1801-1810.
- Taw N. (2010). Biofloc technology expanding at white shrimp farms. Glob. Aquac. Advocate 13: 20–22.
- Vungarala H., Sudhagar A., Reddy A.K., Majeedkutty R.A.B. (2021). Growth, body composition and antioxidant status of Litopenaeus vannamei juveniles reared at different stocking densities in the biofoc system using inland saline groundwater. Aquac. Res., 52: 6299–6307.
- Wasielesky W., Atwood H., Stokes A., Browdy C.L. (2006). Effect of natural production in a zero exchange suspended microbial floc based super-intensive culture system for white shrimp Litopenaeus vannamei. Aquaculture, 258: 396–403.
- Wasielesky W., Froes C., Fóes G., Krummenauer D., Lara G., Poersch L. (2013). Nursery of Litopenaeus vannamei reared in a biofoc system: Te efect of stocking densities and compensatory growth. J. Shellfsh Res., 32: 799–806.
- Xu W.J., Morris T.C., Samocha T.M. (2018). Effects of two commercial feeds for semi-intensive and hyper-intensive culture and four C/N ratios on water quality and performance of Litopenaeus vannamei juveniles at high density in biofloc-based, zero-exchange outdoor tanks. Aquaculture, 490: 194–20.
- Xu W.J., Pan L.Q. (2012). Effects of bioflocs on growth performance, digestive enzyme activity and body composition of juvenile Litopenaeus vannamei in zero-water exchange tanks manipulating C/N ratio in feed. Aquaculture, 356: 147-152.
- Xu W.J., Pan L.Q. (2013) Enhancement of immune response and antioxidant status of Litopenaeus vannamei juvenile in biofloc-based culture tanks manipulating high C/N ratio of feed input. Aquaculture, 412–413: 117–124.
- Xu Z., Guan W., Xie, D., Lu W., Ren X., Yuan J., Mao L. (2019). Evaluation of immunological response in shrimp Penaeus vannamei submitted to low temperature and air exposure. Dev. Comp. Immunol., 100: 103413
- Yang S.P., Wu Z.H., Jian J.C., Zhang X.Z. (2010). Effect of marine red yeast Rhodosporidium paludigenum on growth and antioxidant competence of Litopenaeus vannamei. Aquaculture, 309: 62–65.
- Yin Z., Lam,T.J., Sin Y.M. (1995). The effects of crowding stress on the non-specific immuneresponse in fancy carp (Cyprinus carpio L.). Fish. Shellfish Immunol., 5: 519-529.
- Yu Y., Choi J.H., Lee J., Jo A., Lee J., Choi H.J., Kang Y.J., Choi C.Y., Kang J.C., Lee K.M., Kim J.J. (2024). The use, application and efficacy of biofloc technology (BFT) in shrimp aquaculture industry: A review. Environ. Technol. Innov., 33: 103345.