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Evaluating the Performance of Pacific White Shrimp (Penaeus vannamei Boone, 1931) Reared under Different Stocking Densities: A Study of their Biochemical, Immune, Metabolic and Antioxidant Responses in a Biofloc Aquaculture System Cover

Evaluating the Performance of Pacific White Shrimp (Penaeus vannamei Boone, 1931) Reared under Different Stocking Densities: A Study of their Biochemical, Immune, Metabolic and Antioxidant Responses in a Biofloc Aquaculture System

Open Access
|Apr 2026

Figures & Tables

Table 1.

Characteristics of the treatments used based on different stocking densities, in 35 days of the experiment

Treatment 1Stocking densityNumber of shrimp in the rearing tank (150 L)No.WE (daily)
0.5–1% WESD1120.308 g/LTreatment 1
0.5–1% WESD2240.616 g/LTreatment 2
0.5–1% WESD3360.924 g/LTreatment 3
0.5–1% WESD4481.23 g/LTreatment 4

[i] Abbreviations: number (No), water exchange (WE).

Table 2.

Growth and nutritional performance of P. vannamei reared under different stocking densities in a biofloc system for 5 weeks

ParametersSD1SD2SD3SD4
FBW (g)9.60±0.53 a9.34±0.44 a8.25±0.55 b7.01±0.66 c
BWG (g)5.75±0.53 a5.49±0.45 a4.40±0.55 b3.16±0.66 c
SGR (%/day)2.60±0.16 a2.53±0.13 a2.17±0.19 b1.70±0.27 c
FCR1.20±0.11 c1.27±0.09 c1.69±0.22 b2.34±0.55 a
FE (%)83.92±7.73 a78.99±6.50 a59.88±7.54 b44.59±9.29 c
SR (%)97.22±4.30 a95.83±3.72 a90.74±1.43 b88.19±2.84 c

[i] Abbreviations: final body weight (FBW), body weight gain (BWG), specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), survival rate (SR).

[ii] Values are expressed as mean ± SD. Values in the same row with different letters are significantly different (P<0.05).

Figure 1.

Biochemical activity of P. vannamei hemolymph including glucose (Glu. A), triglyceride (Tg. B), and cholesterol (Chol. C) under the influence of different stocking densities at the end of the experimental period (35 days)

Figure 2.

Immunity activities in the hemolymph of P. vannamei including lysozyme (Lys. A), and phenoloxidase (PO, B), under the influence of different stocking densities at the end of the experimental period (35 days)

Figure 3.

Metabolic enzyme activities (A: ALP, B: ALT, C: AST) in the hepatopancreas of P. vannamei at the end of the experimental period under the influence of different stocking densities at the end of the experimental period (35 days)

Figure 4.

Antioxidant enzyme activities (A: CAT, B: GPx, C: SOD) in the hepatopancreas of P. vannamei at the end of the experimental period under the influence of different stocking densities at the end of the experimental period (35 days)

DOI: https://doi.org/10.2478/aoas-2025-0087 | Journal eISSN: 2300-8733 (formerly 1642-3402) | Journal ISSN: 1642-3402
Language: English
Page range: 813 - 823
Submitted on: Jun 11, 2025
Accepted on: Aug 8, 2025
Published on: Apr 30, 2026
Published by: National Research Institute of Animal Production
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
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© 2026 Mohammad Hossein Khanjani, published by National Research Institute of Animal Production
This work is licensed under the Creative Commons Attribution 4.0 License.