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Employing induced Pluripotent Stem Cells for Primordial Germ Cells Generation in mammalian Wildlife: Challenges and Opportunities Cover

Employing induced Pluripotent Stem Cells for Primordial Germ Cells Generation in mammalian Wildlife: Challenges and Opportunities

Open Access
|Aug 2025

Abstract

Induced pluripotent stem cells (iPSCs) are obtained from adult somatic cells and can be differentiated into multiple cell types. The molecular mechanisms of iPSCs in wild animals are only recently being explored. These mechanisms are species-specific, which complicates the broader application of iPSC technologies across wild species, conversely, in domestic species, several reports mentioned the success on iPSCs obtention. Additionally, iPSCs can be differentiated into primordial germ cells (PGCs) which have the potential to be differentiated into gametes in vitro and in vivo. Many studies report the obtention of PGCs in domestic animals, however, only a few reports describe PGCs obtention in wild species. Dilucidated the mechanisms involved in PGCs obtention from iPSCs in different animal species potentially lead to the development of a valuable tool for animal conservation. This review aims to explore different aspects of iPSCs and PGCs technology, highlighting progress and techniques in obtaining these cells from endangered and wild animals while also comparing these findings with those from domestic animals.

DOI: https://doi.org/10.2478/aoas-2025-0073 | Journal eISSN: 2300-8733 | Journal ISSN: 1642-3402
Language: English
Submitted on: Aug 6, 2024
Accepted on: Jun 27, 2025
Published on: Aug 26, 2025
Published by: National Research Institute of Animal Production
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Constanza Aguilera, Yat Sen Wong, Diana Echeverry, Daniel Veraguas-Dávila, Daniela Cartes, published by National Research Institute of Animal Production
This work is licensed under the Creative Commons Attribution 4.0 License.

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