Evaluation of silkworm pupae meal and fish protein hydrolysate as a sustainable fish meal replacement in striped murrel (Channa striata) diet: impact on growth performance, enzyme activity and IGF-1 gene expression

Sathishkumar, Govindharaj; Felix, Nathan; Ranjan, Amit; Uma, Arumugam; Rajalakshmi, Kalaivanan

doi:10.2478/aoas-2025-0041

Abstract

Silkworm pupae meal (SWP) and fish protein hydrolysate (FPH) are promising alternative protein sources due to their high nutrient availability and sustainability, which contribute to improved growth performance in aquaculture species. This study evaluated the effects of dietary SWP individually and in combination with FPH on growth, nutrient utilization, antioxidant capacity, digestive and metabolic enzyme activities, histology, haemato-biochemical profile, and growth gene expression (IGF-1) of striped murrel (Channa striata). Fish were fed ad libitum with five experimental diets: control (35% fishmeal (FM)), 25 SWP (25% FM replaced with SWP), 50 SWP (50% FM replaced with SWP), 25 SWP+FPH (25% FM replaced with a combination of SWP and 3.5% FPH) and 50 SWP+FPH (50% FM replaced with a combination of SWP and 3.5% FPH). Each diet was fed to triplicate groups of 30 fish per cage for a period of 60 days, following a completely randomized design (CRD). Among the dietary groups, replacing FM with up to 50% SWP supplemented with 3.5% FPH (50SWP+FPH) diet did not negatively affect the growth performance and nutrient utilization efficiency in striped murrel. Additionally, fish fed a diet containing up to 50% SWP supplemented with FPH showed no negative effects on amylase, protease, and lipase activities, as well as in catalase (CAT), superoxide dismutase (SOD), villi length, and villi width compared to the control diet. However, no significant differences (p > 0.05) were observed in whole-body proximate composition, haemato-biochemical parameters, and the activities of glutathione peroxidase (GPx) and metabolic enzyme activities among fish fed different levels of SWP alone and supplemented with FPH diets. The relative mRNA expression of IGF-1 was significantly higher (p < 0.05) in fish fed the control and 50 SWP+FPH diets compared to other dietary groups. In conclusion, replacing 50% of FM with SWP supplemented with FPH is feasible and does not negatively impact growth, nutrient utilization, whole-body composition, digestive enzyme activities, intestinal histology, haematological and serum biochemical profiles, and IGF-1gene expression in striped murrel (C. striata).

References

Aebi H. (1984). [13] Catalase in vitro. In Methods in enzymology. Elsevier., 105: 121–126.
Search in Google Scholar Back to article
Altomare A.A., Baron G., Aldini G., Carini M., D’Amato A. (2020). Silkworm pupae as source of high-value edible proteins and of bioactive peptides. Food Sci. Nutr., 8: 2652–2661.
Search in Google Scholar Back to article
Anderson D., Siwicki A. (1995). Basic hematology and serology for fish health programs.
Search in Google Scholar Back to article
AOAC (2010). Official Methods of Analysis of Association of Official Analytical Chemists. 18th edn. Washington DC.
Search in Google Scholar Back to article
APHA (2012). Standard methods for the examination of water and wastewater. American Public Health Association., 22nd ed., New York, USA.
Search in Google Scholar Back to article
Battampara P., Sathish T.N., Reddy R., Guna V., Nagananda G.S., Reddy N., Ramesha B.S., Maharaddi V.H., Rao A.P., Ravikumar H.N., Biradar A., Radhakrishna P.G. (2020). Properties of chitin and chitosan extracted from silkworm pupae and egg shells. Int. J. Biol. Macromol., 161: 1296–1304.
Search in Google Scholar Back to article
Beauchamp C., Fridovich I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem., 44: 276–287.
Search in Google Scholar Back to article
Belghit I., Liland N.S., Gjesdal P., Biancarosa I., Menchetti E., Li Y., Waagbø R., Krogdahl Å., Lock E.J. (2019). Black soldier fly larvae meal can replace fish meal in diets of sea-water phase Atlantic salmon (Salmo salar). Aquaculture, 503: 609–619.
Search in Google Scholar Back to article
Bradford M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248–254.
Search in Google Scholar Back to article
Bui H.T.D., Khosravi S., Fournier V., Herault M., Lee K.J. (2014). Growth performance, feed utilization, innate immunity, digestibility and disease resistance of juvenile red seabream (Pagrus major) fed diets supplemented with protein hydrolysates. Aquaculture, 418: 11–16.
Search in Google Scholar Back to article
Cardinaletti G., Randazzo B., Messina M., Zarantoniello M., Giorgini E., Zimbelli A., Bruni L., Parisi G., Olivotto I., Tulli F. (2019). Effects of graded dietary inclusion level of full-fat Hermetia illucens prepupae meal in practical diets for rainbow trout (Oncorhynchus mykiss). Animals, 9: 251.
Search in Google Scholar Back to article
Chaklader M.R., Howieson J., Siddik M.A., Foysal M.J., Fotedar R. (2021). Supplementation of tuna hydrolysate and insect larvae improves fishmeal replacement efficacy of poultry by-product in Lates calcarifer (Bloch, 1790) juveniles. Sci. Rep., 11: 4997.
Search in Google Scholar Back to article
Chandhini S., Trumboo B., Jose S., Varghese T., Rajesh M., Kumar V.R. (2021). Insulin-like growth factor signalling and its significance as a biomarker in fish and shellfish research. Fish Physiol. Biochem., 47: 1011–1031.
Search in Google Scholar Back to article
Chemello G., Renna M., Caimi C., Guerreiro I., Oliva-Teles A., Enes P., Biasato I., Schiavone A., Gai F., Gasco L. (2020). Partially defatted Tenebrio molitor larva meal in diets for grow-out rainbow trout, Oncorhynchus mykiss (Walbaum): effects on growth performance, diet digestibility and metabolic responses. Animals., 10: 229.
Search in Google Scholar Back to article
Cherry I.S., Crandall Jr L.A. (1932). The specificity of pancreatic lipase: its appearance in the blood after pancreatic injury. Am. J. Physiol-Legacy Content., 100(2): 266–273.
Search in Google Scholar Back to article
Chowdhury S., Saikia S.K. (2020). Oxidative Stress in Fish: A Review. J. Sci. Res., 12: 145–160.
Search in Google Scholar Back to article
Damodaran S, Parkin K.L. (2017). Amino acids, peptides, and proteins. In Fennema’s food chemistry., CRC press: 235–356.
Search in Google Scholar Back to article
Das R., Das B.K., Hassan M.A., Krishna G., Chadha N.K., Rawat K.D., Jena K. (2023). Valorization of the insect waste as a source of dietary protein in replacing the fishmeal protein for the cage reared Pangasianodon hypophthalmus: An approach to search the alternate non-conventional feed resource of animal origin. Anim. Feed Sci. Technol., 303: 115691.
Search in Google Scholar Back to article
De Marco G., Cappello T., Maisano M. (2023). Histomorphological changes in fish gut in response to prebiotics and probiotics treatment to improve their health status: A review. Animals., 13: 2860.
Search in Google Scholar Back to article
Drapeau G.R. (1976). [38] Protease from Staphyloccus aureus. In Methods in enzymology, vol 45. Elsevier., 45: 469–475.
Search in Google Scholar Back to article
Duncan D.B. (1955). Multiple range and multiple F tests. Biometrics., 11: 1–42.
Search in Google Scholar Back to article
Fan Z., Wu D., Li C., Zhou M., Wang L., Zhang H., Li J., Rong X., Zhao D., Wang J. (2024). Application of fish protein hydrolysates in common carp (Cyprinus carpio) diets for fish meal sparing: Evidence from growth, intestinal health and microflora composition. Aquac. Rep., 36: 102160.
Search in Google Scholar Back to article
Gangadhar B., Umalatha H., Ganesh H., Saurabh S., Sridhar N. (2017). Digestibility of dry matter and nutrients from three ingredients by the carps, Labeo fimbriatus (Bloch, 1795) and Cyprinus carpio Linnaeus, 1758 with a note on digestive enzyme activity. Indian J. Fish., 64: 75–84.
Search in Google Scholar Back to article
Gasco L., Biasato I., Dabbou S., Schiavone A., Gai F. (2019). Animals fed insect-based diets: State-of-the-art on digestibility, performance and product quality. Animals, 9: 170.
Search in Google Scholar Back to article
Ha N., Jesus G.F.A., Gonçalves A.F.N., de Oliveira N.S., Sugai J.K., Pessatti M.L., Mouriño J.L.P., Fabregat T.E.H.P. (2019). Sardine (Sardinella spp.) protein hydrolysate as growth promoter in South American catfish (Rhamdia quelen) feeding: Productive performance, digestive enzymes activity, morphometry and intestinal microbiology. Aquaculture, 500: 99–106.
Search in Google Scholar Back to article
Hasan I., Gai F., Cirrincione S., Rimoldi S., Saroglia G., Terova G. (2023). Chitinase and insect meal in aquaculture nutrition: A comprehensive overview of the latest achievements. Fishes, 8: 607.
Search in Google Scholar Back to article
Henry M., Gasco L., Piccolo Fountoulaki E. (2015). Review on the use of insects in the diet of farmed fish: Past and future. Anim. Feed. Sci. Technol., 203: 1–22.
Search in Google Scholar Back to article
Hossain M., Latifa G., Rahman M. (2008). Observations on induced breeding of snakehead murrel, Channa striatus (Bloch, 1793). Int. J. Sustain. Crop. Prod., 3: e8.
Search in Google Scholar Back to article
Hua K., Cobcroft J. M., Cole A., Condon K., Jerry D.R., Mangott A., Praeger C., Vucko M.J., Zeng C., Zenger K., Strugnell J.M. (2019). The future of aquatic protein: implications for protein sources in aquaculture diets. One Earth., 1: 316–329.
Search in Google Scholar Back to article
Huntington T.C., Hasan M.R. (2009). Fish as feed inputs for aquaculture–practices, sustainability and implications: a global synthesis. FAO Fisheries and Aquaculture Technical Paper., 518: 1–61.
Search in Google Scholar Back to article
Hussain S.M., Khurram F., Naeem A., Hussain Shah S.Z., Sarker P.K., Naeem E., Arsalan M.Z.U.H., Riaz D., Yousaf Z., Faisal M., Amjad M. (2024). A review on the prospects and potentials of fishmeal replacement with different animal protein sources. Int. Aquat. Res., 16: 7.
Search in Google Scholar Back to article
IACUC F. (2014). Guidelines for the preparation and use of MS222 (TMS, tricaine methanesulfonate) for animal procedures. Florida Atlantic University, USA.
Search in Google Scholar Back to article
Ighodaro O.M., Akinloye O.A. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alex. J. Med., 54: 287–293.
Search in Google Scholar Back to article
Ijaiya A.T., Eko E.O. (2009). Effect of Replacing Dietary Fish Meal with Silkworm (Anaphe infracta) Caterpillar Meal on Performance, Carcass Characteristics and Haematological Parameters of Finishing Broiler Chicken. Pak. J. Nutr., 8: 850–855.
Search in Google Scholar Back to article
Ishida Y., Fujita T., Asai K. (1981). New detection and separation method for amino acids by high-performance liquid chromatography. J. Chromatogr. A., 204: 143–148.
Search in Google Scholar Back to article
Ji H., Zhang J.L., Huang J.Q., Cheng X.F., Liu C. (2015). Effect of replacement of dietary fish meal with silkworm pupae meal on growth performance, body composition, intestinal protease activity and health status in juvenile Jian carp (Cyprinus carpio var. Jian). Aquac. Res., 46: 1209–1221.
Search in Google Scholar Back to article
Kamarudin M.S., Rosle S., Yasin I.S.M. (2021). Performance of defatted black soldier fly prepupae meal as fishmeal replacement in the diet of lemon fin barb hybrid fingerlings. Aquac. Rep., 21: 100775.
Search in Google Scholar Back to article
Karthick Raja P., Aanand S., Stephen Sampathkumar J., Padmavathy P. (2019). Silkworm pupae meal as alternative source of protein in fish feed. J. Entomol. Zool. Stud., 7: 78–85.
Search in Google Scholar Back to article
Kristinsson H.G., Rasco B.A. (2000). Fish Protein Hydrolysates: Production, Biochemical, and Functional Properties. Crit. Rev. Food. Sci. Nutr., 40: 43–81.
Search in Google Scholar Back to article
Kumar R., Gokulakrishnan M.G., Debbarma J., Damle D.K. (2022). Advances in captive breeding and seed rearing of striped murrel Channa striata, a high value food fish of Asia. Anim. Reprod. Sci., 238: 106957.
Search in Google Scholar Back to article
Kurbanov A.R., Milusheva R.Y., Rashidova S.S., Kamilov B.G. (2015). Effect of replacement of fish meal with silkworm (Bombyx mori) pupa protein on the growth of Clarias gariepinus fingerling. Int. J. Fish. Aquat. Stud., 2: 25–27.
Search in Google Scholar Back to article
Kouřimská L., Adámková A. (2016). Nutritional and sensory quality of edible insects. J. Nutr. Food Secur., 4: 22–26.
Search in Google Scholar Back to article
Lee J., Choi I.C., Kim K.T., Cho S.H., Yoo J.Y. (2012). Response of dietary substitution of fishmeal with various protein sources on growth, body composition and blood chemistry of olive flounder (Paralichthys olivaceus, Temminck & Schlegel, 1846). Fish Physiol. Biochem., 38: 735–744.
Search in Google Scholar Back to article
Livak K.J., Schmittgen T.D. (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods., 25: 402–408.
Search in Google Scholar Back to article
Longvah T., Mangthya K., Ramulu P. (2011). Nutrient composition and protein quality evaluation of eri silkworm (Samia ricinii) prepupae and pupae. Food Chem., 128: 400–403.
Search in Google Scholar Back to article
Luo F.Y., Ning X.L., Wu G.Z., Li B.X., Li Y.Y., He J.F. (2010). Study on antiseptic antibacterial effect of natural antimicrobial peptides from silkworm pupa on the fresh pork. Sci. Technol. Food Ind., 3: 117–118.
Search in Google Scholar Back to article
Mahato I.S., Timalsina P., Paudel K., Shrestha A., Bhusal C., Kunwar P. (2023). Effects of replacing dietary shrimp meal and soybean meal with silkworm (Bombyx mori) pupae meal on growth performance of rainbow trout Oncorhynchus mykiss. Int. J. Fish. Aqua. Stud., 11: 21–25.
Search in Google Scholar Back to article
Makkar H.P., Tran G., Heuzé V., Ankers P. (2014). State-of-the-art on use of insects as animal feed. Anim. Feed sci. Technol., 197: 1–33.
Search in Google Scholar Back to article
Mishraa, N., Hazarikaa, N.C., Naraina, K., & Mahanta, J. (2003). Nutritive value of non-mulberry and mulberry silkworm pupae and consumption pattern in Assam. India. Nutr. Res., 23: 1303–1311.
Search in Google Scholar Back to article
Morales A.E., García-Rejón L., De la Higuera M. (1990). Influence of handling and/or anaesthesia on stress response in rainbow trout. Effects on liver primary metabolism. Comp. Biochem. Physiol. A-Mol. Integr. Physiol., 95: 87–93.
Search in Google Scholar Back to article
Mousavi S., Zahedinezhad S., Loh J.Y. (2020). A review on insect meals in aquaculture: The immunomodulatory and physiological effects. Int. Aqua. Res., 12: 100–115.
Search in Google Scholar Back to article
Musa, A.F., Cheang J.M. (2022). Haruan Extract (Channa striatus) as an effective mediator in promoting wound healing. Skin Grafts for Successful Wound Closure., 1: 29.
Search in Google Scholar Back to article
Nandeesha M.C., Gangadhara B., Varghese T.J. and Keshavanath P. (2000). Growth response and flesh quality of common carp, Cyprinus carpio fed with high levels of non-defatted silkworm pupae. Asian Fish. Sci., 13: 235–242.
Search in Google Scholar Back to article
Ni H., Chen H. X., Yang Y.Y., Tao L. (1998). Studies on extraction and preparation technique of silkworm chrysalis (Bombyx mori L.) pupa chitin and chitosan. Journal of Hubei University (Natural Science Edition)., 20: 94–96.
Search in Google Scholar Back to article
Nowak V., Persijn D., Rittenschober D., Charrondiere U.R. (2016). Review of food composition data for edible insects. Food Chem., 193: 39–46.
Search in Google Scholar Back to article
Ochoa S. (1955). Malic dehydrogenase and ‘malic’enzyme. Methods of Enzymology., 1: 735–745.
Search in Google Scholar Back to article
Olsen R.L., Hasan M.R. (2012). A limited supply of fishmeal: Impact on future increases in global aquaculture production. Trends in Food Sci. Technol., 27: 120–128.
Search in Google Scholar Back to article
Oso J. (2014). Growth Performance and Nutrient Utilization Efficiency of Clarias gariepinus Juveniles Fed Bombyx mori (Mulberry silkworm) Meal as a Partial Replacement for Fishmeal. Br. J. Appl. Sci. Technol., 4: 3805–3812.
Search in Google Scholar Back to article
Petrova I., Tolstorebrov I., Eikevik T.M. (2018). Production of fish protein hydrolysates step by step: Technological aspects, equipment used, major energy costs and methods of their minimizing. Int. Aqua. Res., 10: 223–241.
Search in Google Scholar Back to article
Rahimnejad S., Hu S., Song K., Wang L., Lu K., Wu R., Zhang C. (2019). Replacement of fish meal with defatted silkworm (Bombyx mori L.) pupae meal in diets for Pacific white shrimp (Litopenaeus vannamei). Aquaculture, 510: 150–159.
Search in Google Scholar Back to article
Rahimnejad S., Lee S.M., Park H.G., Choi J. (2017). Effects of dietary inclusion of Chlorella vulgaris on growth, blood biochemical parameters, and antioxidant enzyme activity in olive flounder, Paralichthys olivaceus. J. World Aqua. Soc., 48: 103–112.
Search in Google Scholar Back to article
Ramji B., Samraj A., Kumar S.S. (2024). Performance of crustacean and insect meal-based diets on the growth and digestive enzyme profile of pearlspot Etroplus suratensis (Bloch, 1790): Influence of crustacean and insect meal on pearlspot, Etroplus suratensis. Indian J. Fish., 71.
Search in Google Scholar Back to article
Rao K.S.P., Rao K.R. (1987). The possible role of glucose-6-phosphate dehydrogenase in the detoxification of methyl parathion. Toxicol. Lett., 39: 211–214.
Search in Google Scholar Back to article
Rick W., Stegbauer H.P. (1974). α-Amylase measurement of reducing groups. In Methods of enzymatic analysis. Elsevier, pp. 885–890.
Search in Google Scholar Back to article
Salem M., Khalafalla M.M.E., Saad I.A.I., El-Hais A.M.A. (2008). Replacement of fish meal by silkworm Bombyx mori pupae meal in Nile tilapia, Oreochromis niloticus diets. Egyp. J. Nutr. Feeds., 11: 611–624.
Search in Google Scholar Back to article
Samantaray K., Mohanty S.S. (1997). Interactions of dietary levels of protein and energy on fingerling snakehead, Channa striata. Aquaculture, 156: 241–249.
Search in Google Scholar Back to article
Sankaran K., Gurnani S. (1972). On the variation in the catalytic activity of lysozyme in fishes. Indian J. Biochem. Biophys., 9: 162–165.
Search in Google Scholar Back to article
Sathishkumar G., Felix N., Prabu E. (2021). Growth performances and nutrient utilization efficiency of GIFT tilapia reared in floating net cages fed with bioprocessed silkworm pupae meal. Aquac. Nutr., 27: 2786–2797.
Search in Google Scholar Back to article
Shakoori M., Gholipour H., Naseri S., Khara H. (2016). Growth, survival, and body composition of rainbow trout, when dietary fish meal is replaced with silkworm pupae. Fish. Aquat. Life., 24: 53–57.
Search in Google Scholar Back to article
Siddaiah G.M., Kumar R., Kumari R., Damle D.K., Rasal K.D., Manohar V., Sundaray J.K., Pillai B.R. (2022). Dietary supplementation of fish protein hydrolysate improves growth, feed efficiency and immune response in freshwater carnivore fish, Channa striata fingerlings. Aquac. Res., 53: 3401–3415.
Search in Google Scholar Back to article
Siddik M.A.B., Howieson J., Fotedar R. (2019). Beneficial effects of tuna hydrolysate in poultry by-product meal diets on growth, immune response, intestinal health and disease resistance to Vibrio harveyi in juvenile barramundi, Lates calcarifer. Fish & Shellfish Immunol., 89: 61–70.
Search in Google Scholar Back to article
Sridharan J., Samraj A., John S.S.K., Antony C. (2023). Effect of replacement of dietary fish meal with silkworm pupae meal and black soldier fly larvae meal as a combination diet on the growth and digestive performance of koi carp Cyprinus carpio var. Koi in the nursery phase. Indian J. Fish., 70.
Search in Google Scholar Back to article
Suratip N., Charoenwattanasak S., Klahan R., Herault M., Yuangsoi B. (2023). An investigation into the effects of using protein hydrolysate in low fish meal diets on growth performance, feed utilization and health status of snakehead fish (Channa striata) fingerling. Aquac. Rep., 30: 101623.
Search in Google Scholar Back to article
Takahashi-Íñiguez T., Aburto-Rodríguez N., Vilchis-González A.L., Flores M.E. (2016). Function, kinetic properties, crystallization, and regulation of microbial malate dehydrogenase. J. Zhejiang Univ. Sci., 17: 247–261.
Search in Google Scholar Back to article
Vikas P.A. (2023). Biometric and fatty acid profile of the brine shrimp Artemia franciscana enriched with marine microalgal species belonging to prymnesiophytes and eustigmatophytes. J. Krishi. Vigyan., 11: 15–20.
Search in Google Scholar Back to article
Vikas P.A., Chakraborty K., Sajeshkumar N.K., Thomas P.C., Sanil N.K., Vijayan K.K. (2014). Quality of six Indian populations of Artemia franciscana for larval finfish culture. J. Appl. Aquac., 26: 271–291.
Search in Google Scholar Back to article
Wang G.J., Yin W.F., Wang J., Tang J.L., Huang Y.P. (2007). Effect of polysaccharide of silkworm pupa on immunological function in mice. Journal of Jiangsu University (Medicine Edition)., 17: 373–375.
Search in Google Scholar Back to article
Wei Y., Wang J., Zhang X., Duan M., Jia L., Xu H., Liang M., Liu J. (2021). Fish protein hydrolysate supplementation in plant protein-based diets for tiger puffer (Takifugu rubripes) is an effective strategy of fish meal sparing. Aquac. Rep., 20: 100720.
Search in Google Scholar Back to article
Wendel A. (1981). Glutathione peroxidase. In Methods in enzymology. Elsevier., 77: 325–333.
Search in Google Scholar Back to article
Wu X., He K., Velickovic T.C., Liu Z. (2021). Nutritional, functional, and allergenic properties of silkworm pupae. Food Sci. Nutr., 9: 4655–4665.
Search in Google Scholar Back to article
Xu X., Ji H., Yu H., Zhou J. (2018). Influence of replacing fish meal with enzymatic hydrolysates of defatted silkworm pupa (Bombyx mori L.) on growth performance, body composition and non‐specific immunity of juvenile mirror carp (Cyprinus carpio var. Specularis). Aquac. Res., 49: 1480–1490.
Search in Google Scholar Back to article
Yue Y., Cui J. A., Chen X.P. (2013). Separation purification antimicrobial peptides from silkworm chrysalis and physical and chemical properties. Food and Fermentation Technology., 49: 62–67.
Search in Google Scholar Back to article
Zhang Q., Bian Y., Zhao Y., Xu Y., Wu J., Wang D., Wang J., Wang A., Qi Z. (2022). Replacement of fishmeal by fermented silkworm pupae meal in diets of largemouth bass (Micropterus salmoides): Effects on growth performance and feed utilization. J. Appl. Ichthyol., 38: 579–585.
Search in Google Scholar Back to article
Zhang M., Haga A., Sekiguchi H., Hirano S. (2000). Structure of insect chitin isolated from beetle larva cuticle and silkworm (Bombyx mori) pupa exuvi. Int. J. Biol. Macromol., 27: 99–105.
Search in Google Scholar Back to article
Zhou J. S., Chen Y.S., Ji H., Yu E.M. (2017). The effect of replacing fish meal with fermented meal mixture of silkworm pupae, rapeseed and wheat on growth, body composition and health of mirror carp (Cyprinus carpio var. Specularis). Aquac. Nutr., 23: 741–754.
Search in Google Scholar Back to article
Zhou Q.C., Mai K.S., Tan B.P., Liu Y.J. (2005). Partial replacement of fishmeal by soybean meal in diets for juvenile cobia (Rachycentron canadum). Aquac. Nutr., 11: 175–182.
Search in Google Scholar Back to article
Zuo X., Woo P.T.K. (1997). Natural anti-proteases in rainbow trout, Oncorhynchus mykiss and brook charr, Salvelinus fontinalis and the in vitro neutralization of fish α2-macroglobulin by the metalloprotease from the pathogenic haemoflagellate, Cryptobia salmositica. Parasitology., 114: 375–382.
Search in Google Scholar Back to article

Evaluation of silkworm pupae meal and fish protein hydrolysate as a sustainable fish meal replacement in striped murrel (Channa striata) diet: impact on growth performance, enzyme activity and IGF-1 gene expression

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