Abstract
The urinary tract microbiota (urobiota) in healthy individuals remains understudied, particularly in men. Methods: Expanded quantitative urine cultures were performed on 40 asymptomatic, presumably healthy males (18–40 years). Results: 88% of samples showed bacterial growth, predominated by Firmicutes (Streptococcus, Staphylococcus, Corynebacterium). Sexual behavior influenced diversity. Conclusions: The male urinary tract harbors a complex microbiota, challenging the “sterile urine” paradigm.