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CRISPR/Cas System: An Effective Tool Against Pathogenic Diseases Cover

CRISPR/Cas System: An Effective Tool Against Pathogenic Diseases

Advancements of Microbiology
Volume 62 (2023): Issue 2 (June 2023)
By: Shakira Aslam,  Ali Umair,  Zaid Aslam,  Muhammad Zafar Saleem and  Hamid Bashir  
Open Access
|Jun 2023

Figures & Tables

Fig. 1.

CRISPR/Cas system mechanism of action
CRISPR/Cas system mechanism of action

Fig. 2.

Anti-SARS-CoV-2 effect produced by CRISPR/Cas13d that inhibits the replication of virus by crRNA-guided viral mRNA and genome degradation
Anti-SARS-CoV-2 effect produced by CRISPR/Cas13d that inhibits the replication of virus by crRNA-guided viral mRNA and genome degradation

Fig. 3.

Mechanism of ABACAS strategy. ABACAS strategy captures S protein of Coronavirus and successfully delivers the components of CRISPR into infected cells. CRISPR components then attack viral RNA and degrade it. This strategy also helps in preventing viral entry into cell
Mechanism of ABACAS strategy. ABACAS strategy captures S protein of Coronavirus and successfully delivers the components of CRISPR into infected cells. CRISPR components then attack viral RNA and degrade it. This strategy also helps in preventing viral entry into cell

Fig. 4.

CRISPR-Cas strategy against herpes virus [108]
CRISPR-Cas strategy against herpes virus [108]

Fig. 5.

Limitations of CRISPR/Cas
Limitations of CRISPR/Cas

CRISPR/Cas systems for elimination and targeting HIV-1 provirus

CRISSPR-Cas system usedDelivery method for CRISPR componentsTargeted regionsCell line or organism studiedTargeted locusEfficiencyReferences
SpCas9TransfectionLTR (U3 region)293T, Jurkat & Hela465–48430–90%[104]
SpCas9TransfectionLTR (U3 region)CHME5, TZM-BI, & U937101–127312–34130–90%[105]
SpCas9LentivirusLTR (R region)293T-CD4-CCR5, hPSC, & 293 Primary T cells464–486485–50720–90%[107]
SpCas9TransfectionRev (the second exon)JLat10.68513–853230%[93]
SpCas9LentivirusGag/ Pol/ Rev/ EnvSupT12249–22778497–852530–90%[135]
SpCas9LentivirusLTR (U3 and R region)J.Lat FL & SupT1300–408463–48235–98%[108]
DOI: https://doi.org/10.2478/am-2023-0009 | Journal eISSN: 2545-3149 | Journal ISSN: 0079-4252
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Language: English, Polish
Page range: 87 - 99
Submitted on: Nov 1, 2022
|
Accepted on: Dec 1, 2022
|
Published on: Jun 26, 2023
Published by: Polish Society of Microbiologists
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
AIDS,
ABACAS,
COVID-19,
CRISPR/Cas system,
DETECTR,
Herpesvirus,
PAC-MAN,
SHERLOCK
Related subjects:
Life sciences,
Microbiology and virology

© 2023 Shakira Aslam, Ali Umair, Zaid Aslam, Muhammad Zafar Saleem, Hamid Bashir, published by Polish Society of Microbiologists
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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