Have a personal or library account? Click to login
Effects of N-alkyl quaternary quinuclidines on oxidative stress biomarkers in SH-SY5Y cells Cover

Effects of N-alkyl quaternary quinuclidines on oxidative stress biomarkers in SH-SY5Y cells

Archives of Industrial Hygiene and Toxicology
Volume 76 (2025): Issue 3 (September 2025)
By: Antonio Zandona,  Lucija Marcelić,  Suzana Žunec,  Josip Madunić and  Maja Katalinić  
Open Access
|Sep 2025

Figures & Tables

Figure 1

Lipid peroxidation (expressed as TBARS concentrations) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. Control – untreated cells; H2O2 – positive control (100 µmol/L)
Lipid peroxidation (expressed as TBARS concentrations) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. Control – untreated cells; H2O2 – positive control (100 µmol/L)

Figure 2

DCFDA- or DAF-FM fluorescence (expressed as RFUs normalised to control) indicating ROS (left panel) and RNS (right panel) levels in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h or 1 h, respectively. $ p<0.001; *p<0.0001 (Dunnett’s test). Control – untreated cells; DAF-FM – 4-amino-5-methylamino-2,7-difluorofluorescein diacetate; DCFDA – 2′,7′-dichlorofluorescein diacetate dye; H2O2 – positive control (100 µmol/L); RFU – relative fluorescence units; SNP – positive control (200 µmol/L)
DCFDA- or DAF-FM fluorescence (expressed as RFUs normalised to control) indicating ROS (left panel) and RNS (right panel) levels in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h or 1 h, respectively. $ p<0.001; *p<0.0001 (Dunnett’s test). Control – untreated cells; DAF-FM – 4-amino-5-methylamino-2,7-difluorofluorescein diacetate; DCFDA – 2′,7′-dichlorofluorescein diacetate dye; H2O2 – positive control (100 µmol/L); RFU – relative fluorescence units; SNP – positive control (200 µmol/L)

Figure 3

Total antioxidative capacity (TAC, expressed as µmol/L of Fe2+ equivalents, left panel) and MCB-fluorescence indicating GSH level (expressed as RFU normalised to control, right panel) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. # p<0.01; *p<0.0001 (Dunnett’s test). Control – untreated cells; H2O2 or tBHP– positive controls (100 µmol/L); RFU – relative fluorescence units; SNP – positive control (200 µmol/L)
Total antioxidative capacity (TAC, expressed as µmol/L of Fe2+ equivalents, left panel) and MCB-fluorescence indicating GSH level (expressed as RFU normalised to control, right panel) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. # p<0.01; *p<0.0001 (Dunnett’s test). Control – untreated cells; H2O2 or tBHP– positive controls (100 µmol/L); RFU – relative fluorescence units; SNP – positive control (200 µmol/L)

Figure 4

Cytosolic (Cu/Zn-SOD, left panel) and mitochondrial (Mn-SOD, right panel) superoxide dismutase activity (expressed as U/mg protein) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. & p<0.05; #p<0.01; $p<0.001; *p<0.0001 (Dunnett’s test). Control – untreated cells; tBHP– positive control (100 µmol/L)
Cytosolic (Cu/Zn-SOD, left panel) and mitochondrial (Mn-SOD, right panel) superoxide dismutase activity (expressed as U/mg protein) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. & p<0.05; #p<0.01; $p<0.001; *p<0.0001 (Dunnett’s test). Control – untreated cells; tBHP– positive control (100 µmol/L)

Figure 5

DNA damage (expressed as percentage normalised to the control) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. *p<0.0001 (Dunnett’s test). Control – untreated cells; Etoposide – positive control (10 µmol/L)
DNA damage (expressed as percentage normalised to the control) in SH-SY5Y cells treated with 60 µmol/L QOH-C12, 6 µmol/L QOH-C14 or QOH-C16, 100 µmol/L QNOH-C12, and 25 µmol/L QNOH-C14 or QNOH-C16 for 4 h. *p<0.0001 (Dunnett’s test). Control – untreated cells; Etoposide – positive control (10 µmol/L)
DOI: https://doi.org/10.2478/aiht-2025-76-4007 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Journal RSS Feed
Language: English, Croatian, Slovenian
Page range: 203 - 210
Submitted on: Jun 1, 2025
|
Accepted on: Jul 1, 2025
|
Published on: Sep 30, 2025
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
DNA damage,
glutathione,
mitochondrial dysfunction,
oximes,
reactive nitrogen species,
reactive oxygen species,
superoxide dismutase
Related subjects:
Medicine,
Basic medical science,
Basic medical science, other

© 2025 Antonio Zandona, Lucija Marcelić, Suzana Žunec, Josip Madunić, Maja Katalinić, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous articleVolume 76 (2025): Issue 3 (September 2025)Next article