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Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines Cover

Cytotoxic activity of strawberry tree (Arbutus unedo L.) honey, its extract, and homogentisic acid on CAL 27, HepG2, and Caco-2 cell lines

Open Access
|Jul 2022

Figures & Tables

Figure 1

Cell viability of CAL 27 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). # P<0.05 vs all AFH concentrations
Cell viability of CAL 27 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). # P<0.05 vs all AFH concentrations

Figure 2

Cell viability of HepG2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH)* P<0.05 vs other STH concentrations. # P<0.05 vs all AFH concentrations. & P<0.05 vs all HGA concentrations
Cell viability of HepG2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH)* P<0.05 vs other STH concentrations. # P<0.05 vs all AFH concentrations. & P<0.05 vs all HGA concentrations

Figure 3

Cell viability of Caco-2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). * P<0.05 vs all STH concentrations. # P<0.05 vs all AFH concentrations
Cell viability of Caco-2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). * P<0.05 vs all STH concentrations. # P<0.05 vs all AFH concentrations

Figure 4

ROS induction in CAL 27 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). # P<0.05 vs all AFH concentrations. $ P<0.05 vs all STHE and HGA Concentrations. & P<0.05 vs other HGA concentrations
ROS induction in CAL 27 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). # P<0.05 vs all AFH concentrations. $ P<0.05 vs all STHE and HGA Concentrations. & P<0.05 vs other HGA concentrations

Figure 5

ROS induction in HepG2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). * P<0.05 vs all STH concentrations. # P<0.05 vs all AFH concentrations
ROS induction in HepG2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). * P<0.05 vs all STH concentrations. # P<0.05 vs all AFH concentrations

Figure 6

ROS induction in Caco-2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). * P<0.05 vs all STH concentrations. # P<0.05 vs all AFH concentrations
ROS induction in Caco-2 cells after 30 min, 1 h, and 2 h of treatment with strawberry tree honey (STH), strawberry tree honey extract (STHE), homogentisic acid (HGA), and artificial honey (AFH). * P<0.05 vs all STH concentrations. # P<0.05 vs all AFH concentrations

Experimental design

Experimental groupTested concentrations*#
0.5 ×1 ×2.5 ×5 ×
AHF – Artificial honey (g/L)0.350.711.773.5
STH – Strawberry tree honey (g/L)0.350.711.773.5
STHE – Strawberry tree honey extract (mg/L)1.252.56.2512.5
HGA – Homogentisic acid (mg/L)0.1070.2140.5351.07
Exposure
30 min1 h2 h
Cell cultures
HepG2Caco-2CAL 27
DOI: https://doi.org/10.2478/aiht-2022-73-3653 | Journal eISSN: 1848-6312 | Journal ISSN: 0004-1254
Language: English, Croatian, Slovenian
Page range: 158 - 168
Submitted on: Apr 1, 2022
Accepted on: May 1, 2022
Published on: Jul 7, 2022
Published by: Institute for Medical Research and Occupational Health
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2022 Andreja Jurič, Ana Huđek Turković, Irena Brčić Karačonji, Saša Prđun, Dragan Bubalo, Ksenija Durgo, published by Institute for Medical Research and Occupational Health
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.