The Choice of Suitable Conditions for Wheat Genetic Transformation

Klčová, Lenka; Ondreičková, Katarína; Mihálik, Daniel; Gubišová, Marcela

doi:10.2478/agri-2019-0003

Abstract

Wheat is one of three most important cereals worldwide. Its production rises every year. There is a possibility to improve quantitative and qualitative parameters by biolistic method of transformation. The process of introduction of desired gene into the wheat genome and plant regeneration is affected by many factors. To identify the suitable conditions, selection system, the influence of donor, plant environment and the regeneration capacity of wheat genotypes were tested. The scutella of immature embryos served as the initial explants. Plant regeneration was achieved by 6 out of 11 genotypes tested. The highest values were reached by the cultivar Ilias. The effect of donor, plant environment was evaluated by two genotypes. Plants from growth chamber appeared to be better source of explants compared to plants grown in the natural conditions. The selection system was optimized as follows: regeneration medium in the dark and subsequently in photoperiod conditions (DR) with 5 mg/l of phosphinotricin (PPT), shoot induction medium (DS) with 7 mg/l of PPT.

References

ABOUSEADAA, H.H. – OSMAN, G.H. – RAMADAN, A.M. – HASSANEIN, S.E. – ABDELSATTAR, M.T. – MORSY, Y.B. – ALAMELDIN, H.F. – EL-GHAREEB, D.K. – NOUR-ELDIN, H.A. – SALEM, R. – GAD, A.A. – ELKHODARY, S.E. – SHEHATA, M.M. – MAHFOUZ, H.M. – EISSA, H.F. – BAHIELDIN, A. 2015. Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects. In BMC Plant Biology, vol. 15, p. 183. DOI: 10.1186/s12870-015-0570-x10.1186/s12870-015-0570-x
Open DOI Search in Google Scholar Back to article
BRUCE, T.J.A. – ARADOTTIR, G.I. – SMART, L.E. – MARTIN, J.L. – CAULFIELD, J.C. – DOHERTY, A. ‒ SPARKS, C.A. ‒ WOODCOCK, C.M. ‒ BIRKETT, M.A. ‒ NAPIER, J.A. – JONES, H.D. – PICKETT, J.A. 2015. The first crop plant genetically engineered to release an insect pheromone for defence. In Scientific Reports, vol. 5, p. 11183. DOI: 10.1038/srep1118310.1038/srep11183
Open DOI Search in Google Scholar Back to article
GREER, M.S. – KOVALCHUK, I. – EUDES, F. 2009. Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum. In New Biotechnology, vol. 26, no. 1‒2, pp. 44‒52. DOI: 10.1016/j.nbt.2009.02.00310.1016/j.nbt.2009.02.003
Open DOI Search in Google Scholar Back to article
GUBIŠOVÁ, M. ‒ MIHÁLIK, D. ‒ KONÔPKOVÁ, Ľ. 2011. Regeneration efficiency of Slovak spring barley cultivars and winter wheat cultivars. In Agriculture (Poľnohospodárstvo), vol. 57, no. 2, pp. 76‒83. ISSN 1338-4376
Search in Google Scholar Back to article
HE, Y. – WANG, Q. – ZENG, J. – SUN, T. – YANG, G.X. – HE, G.Y. 2015. Current status and trends of wheat genetic transformation studies in China. In Journal of Integrative Agriculture, vol. 14, no. 3, pp. 438‒452. DOI: 10.1016/S2095-3119(14)60934-510.1016/S2095-3119(14)60934-5
Open DOI Search in Google Scholar Back to article
KASIRAJAN, L. – BOOMIRAJ, K. – BANSAL, K.C. 2013. Optimization of genetic transformation protocol mediated by biolistic method in some elite genotypes of wheat (Triticum aestivum L.). In African Journal of Biotechnology, vol. 12, no. 6, pp. 531‒538. DOI: 10.5897/AJB12.278510.5897/AJB12.2785
Open DOI Search in Google Scholar Back to article
KASIRAJAN, L. – KOVILPILLAI, B. – BANSAL, K.C. 2014. Mature embryo-derived wheat transformation for tolerance to moisture stress. In Biotechnology Letters, vol. 36, no. 5, pp. 1037‒1041. DOI: 10.1007/s10529-013-1431-z10.1007/s10529-013-1431-z24375233
Open DOI Search in Google Scholar Back to article
LI, J. – YE, X. – AN, B. – DU, L. – XU, H. 2012. Genetic transformation of wheat: current status and future prospects. In Plant Biotechnology Reports, vol. 6, no. 3, pp. 183‒193. DOI: 10.1007/s11816-011-0213-010.1007/s11816-011-0213-0
Open DOI Search in Google Scholar Back to article
MURÍN, R. – MÉSZÁROS, K. – NEMEČEK, P. – KUNA, R. – FARAGÓ, J. 2012. Regeneration of immature and mature embryos from diverse sets of wheat genotypes using media containing different auxins. In Acta Agronomica Hungarica, vol. 60, no. 2, pp. 97‒108. DOI: 10.1556/AAgr.60.2012.2.210.1556/AAgr.60.2012.2.2
Open DOI Search in Google Scholar Back to article
PASTORI, G.M. – WILKINSON, M.D. – STEELE, S.H. – SPARKS, C.A. – JONES, H.D. – PARRY, M.A.J. 2001. Age-dependent transformation frequency in elite wheat varieties. In Journal of Experimental Botany, vol. 52, no. 357, pp. 857‒863. DOI: 10.1093/jexbot/52.357.85710.1093/jexbot/52.357.85711413223
Open DOI Search in Google Scholar Back to article
RANA, I.A. ‒ SALOMON, S. – SCHÄFER, W. – BECKER D. 2014. Downregulation of Glucan Synthase-Like (TaGSL) genes in wheat leads to inhibition of transgenic plant regeneration. In In Vitro Cellular and Developmental Biology – Plants, vol. 50, no. 6, pp. 696‒706. DOI: 10.1007/s11627-014-9636-y10.1007/s11627-014-9636-y
Open DOI Search in Google Scholar Back to article
RASCO-GAUNT, S. – RILEY, A. – CANNELL, M. – BARCELO, P. – LAZZERI, P.A. 2001. Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment. In Journal of Experimental Botany, vol. 52, no. 357, pp. 865‒874. DOI: 10.1093/jexbot/52.357.86510.1093/jexbot/52.357.865
Open DOI Search in Google Scholar Back to article
SHAH, M.M. – KHALID, Q. – KHAN, U.W. – SHAH, S.A.H. – SHAH, S.A. – HASSAN, A. – PERVEZ, A. 2009. Variation in genotypic responses and biochemical analysis of callus induction in cultivated wheat. In Genetics and Molecular Research, vol. 8, no. 3, pp. 783‒793. ISSN 1676-568010.4238/vol8-3gmr513
Search in Google Scholar Back to article
TAMÁS, C. – SZÜCS, P. – RAKSZEGI, M. – TAMAS, L. – BEDÖ, Z. 2004. Effect of combined changes in culture medium and incubation conditions on regeneration from immature embryos of elite varieties of winter wheat. In Plant Cell, Tissue and Organ Culture, vol. 79, no. 1, pp. 39‒44. DOI: 10.1023/B:TICU.0000049447.81409.ed10.1023/B:TICU.0000049447.81409.ed
Open DOI Search in Google Scholar Back to article
VARSHNEY, A. – ALTPETER, F. 2001. Stable transformation and tissue culture response in current European winter wheats (Triticum aestivum L.). In Molecular Breeding, vol. 8, no. 4, pp. 295‒309. DOI: 10.1023/A:101524090101610.1023/A:1015240901016
Search in Google Scholar Back to article
VASIL, V. – CASTILLO, A.M. – FROMM, M.E. – VASIL, I.K. 1992. Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. In Bio-Technology, vol. 10, no. 6, pp. 667‒674. DOI: 10.1038/nbt069266710.1038/nbt0692-667
Search in Google Scholar Back to article
WANG, X. – REN, X. – YIN, G. – WANG, K. – LI, J. – DU, L. – XU, H. – YE, X. 2014. Effects of environmental temperature on the regeneration frequency of the immature embryos of wheat (Triticum aestivum L.). In Journal of Integrative Agriculture, vol. 13, no. 4, pp. 722‒732. DOI: 10.1016/S2095-3119(13)60362-710.1016/S2095-3119(13)60362-7
Open DOI Search in Google Scholar Back to article
ZHANG, K. – LIU, J. – ZHANG, Y. – YANG, Z. – GAO, C. 2015. Biolistic genetic transformation of a wide range of Chinese elite wheat (Triticum aestivum L.) varieties. In Journal of Genetics and Genomics, vol. 42, no. 10, pp. 39‒42. DOI: 10.1016/j.jgg.2014.11.00510.1016/j.jgg.2014.11.00525619601
Search in Google Scholar Back to article

The Choice of Suitable Conditions for Wheat Genetic Transformation

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