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Antibacterial Activity of Green Tea and Peppermint Extracts Against Enterococcus faecalis and the Potential of EGCG in Oral Health Cover

Antibacterial Activity of Green Tea and Peppermint Extracts Against Enterococcus faecalis and the Potential of EGCG in Oral Health

Open Access
|Apr 2025

Figures & Tables

Figure 1.

Percentage contents (mean values) of total hydroxycinnamic derivatives (THDs), total polyphenols (TPs), and tannins (T) in tested lyophilizates.

Figure 2.

Percentage contents (mean values) of total flavonoids expressed as luteolin-7-O-glucoside (L7G; λ = 392 nm) and quercetin (Q; λ = 420) in tested lyophilizates.

Table 1.

The activity of tested lyophilizates and selected secondary metabolites on the Enterococcus faecalis strains expressed as MIC and MBC in mg mL−1.

E. faecalis strainClinical 1Clinical 2Clinical 3CCM 4224
GTWEMIC2.51.251.252.52.52.52.51.252.52.52.52.5
~1.252.52.52.5
MBC555510105105555
~51055
PLWEMIC2.52.52.5
~NDNDND2.5
MBC>5>5>5
~>5
GT + PLWEMIC510102.510105101051010
~10101010
MBC5>10>1010>10>1010>10>1051010
~>10>10>1010
RAMIC5>5>55555>5>52.55>5
~>55>55
MBC>5>5>5>555>5>5>5>55>5
~>55>5>5
ECMIC
>1 mg mL−1>1 mg mL−1>1 mg mL−1>1 mg mL−1
MBC
~>1 mg mL−1>1 mg mL−1>1 mg mL−1>1 mg mL−1
ECGCMIC0.250.50.250.1250.50.50.250.50.50.250.250.5
~0.250.50.50.25
MBC1>0.5>0.51>0.5>0.51>0.5>0.51>0.5>0.5
~1111
CAMIC>12.52.5>12.52.5>11.252.5>11.252.5
~2.52.52.52.5
MBC>12.52.5>12.52.5>11.252.5>11.252.5
~2.52.52.52.5

[i] Peppermint leaves water extract (PLWE), green tea water extract (GTWE), water extract of peppermint and green tea mixture 1:1 (P + GTWE), rosmarinic acid (RA), (−)-epigallocatechin gallate (EGCG), (−)-epicatechin (EC), and caffeic acid (CA)

[ii] MIC – minimal inhibitory concentration, MBC – minimal bactericidal concentration, CCM – Czech Collection of Microorganisms.

DOI: https://doi.org/10.2478/afpuc-2025-0002 | Journal eISSN: 2453-6725 (formerly 1338-6786) | Journal ISSN: 0301-2298
Language: English
Page range: 13 - 19
Submitted on: Feb 3, 2025
Accepted on: Mar 19, 2025
Published on: Apr 19, 2025
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
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© 2025 J. Kováč, L. Slobodníková, B. Nebus, E. Kurin, S. Bittner Fialová, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.