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The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats Cover

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

Open Access
|Apr 2021

Figures & Tables

Table 1

The Result of Beetroot Extraction.

Type of ExtractCharacteristics
Percentage of Yield (%, w/w)Loss of Drying (%, w/w)Ash Content (%, w/w)
DEB1.922.482.16
HEB34.529.899.66
AFHEB24.5513.2719.75

[i] Note: DEB = Dichloromethane Extract of Beetroot; HEB = Hydroethanolic Extract of Beetroot; and AFHEB = Alkaloids-Free Hydroethanolic Extract of Beetroot

Table 2

Phytochemical Screening Results of Beetroot Extracts.

CompoundsDEBHEBAFHEB
Alkaloids++
Flavonoids+++
Tannin++
Phenolic+++
Saponin++
Steroids
Triterpenoids

[i] Note: (+) = detected; (−) = not detected, DEB = Dichloromethane Extract of Beetroot; HEB = Hydroethanolic Extract of Beetroot; and AFHEB = Alkaloids-Free Hydroethanolic Extract of Beetroot

Table 3

Flavonoids and Iron Contents of Beetroot Extracts.

Type of ExtractIron (Fe) content (mg.Kg−1)Flavonoids Total Content (mgQE.g−1)
DEB6.521.66
HEB20.438.35
AFHEB11.306.07

[i] Note: DEB = Dichloromethane Extract of Beetroot; HEB = Hydroethanolic Extract of Beetroot; and AFHEB = Alkaloids-Free Hydroethanolic Extract of Beetroot

Table 4

The Anti-anaemia Activity of Beetroot Extracts.

GroupsBlood Parameters
Erythrocytes (×106μL)Haemoglobin (g.dL−1)Haematocrit (%)
Normal Control7.00 ± 0.78a16.78 ± 0.37a50.55 ± 1.22a
Negative Control4.73 ± 0.95b12.48 ± 0.48b37.82 ± 1.49b
DEB6.31 ± 0.52a14.94 ± 0.46c45.18 ± 1.48c
HEB7.12 ± 0.61a16.66 ± 0.39a50.04 ± 1.22a
AFHEB6.96 ± 0.55a15.46 ± 0.31c46.54 ± 0.86c
Normal Level Value5.0–12.011.1–18.036.0–52.0

[i] Note: Different letters in the same column show significant differences (p < 0.05). DEB = Dichloromethane Extract of Beetroot; HEB = Hydroethanolic Extract of Beetroot; and AFHEB = Alkaloids-Free Hydroethanolic Extract of Beetroot

Figure 1

MDA levels after treatment for 21 days.

DOI: https://doi.org/10.2478/afpuc-2020-0014 | Journal eISSN: 2453-6725 (formerly 1338-6786) | Journal ISSN: 0301-2298
Language: English
Page range: 1 - 7
Submitted on: Jun 19, 2020
Accepted on: Feb 1, 2021
Published on: Apr 2, 2021
Published by: Comenius University in Bratislava, Faculty of Pharmacy
In partnership with: Paradigm Publishing Services
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© 2021 Ni Putu Ermi Hikmawanti, Lusi Putri Dwita, Dimas W. Wisnunanda, Fanny Farista, published by Comenius University in Bratislava, Faculty of Pharmacy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.