The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

Hikmawanti, Ni Putu Ermi; Dwita, Lusi Putri; Wisnunanda, Dimas W.; Farista, Fanny

doi:10.2478/afpuc-2020-0014

Abstract

Aim

evaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris (L.) subsp. vulgaris) on phenylhydrazine-induced rats.

Methods

Male rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg⁻¹ BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg⁻¹ BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test.

Results

The results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05).

Conclusion

The beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.

References

Ahmad, A., Ansari, S. H., Ahamad, J., & Naquvi, K. J. (2013). Pharmacognostic specifications of roots of Beta vulgaris cultivated in India. Asian Journal of Biomedical and Pharmaceutical Sciences, 3(26), 5–10.
Search in Google Scholar Back to article
Azeredo, H. M. C. (2009). Betalains: properties, sources, applications, and stability - a review. International Journal of Food Science and Technology, 44(12), 2365–2376. https://doi.org/10.1111/j.1365-2621.2007.01668.x
Search in Google Scholar Back to article
Benouadah, Z., Mahdeb, N., & Bouzidi, A. (2016). Evaluation of acute and sub-acute toxicity of alkaloids from Datura stramonium sp. In mice. International Journal of Pharmacognosy and Phytochemical Research, 8(11), 1759–1766.
Search in Google Scholar Back to article
Chaddha, S., & Mittal, A. (2016). Role of Flavonoids in the Treatment of Hemolytic Anaemia-a review. European Journal of Pharmaceutical and Medical Research, 3(5), 212–216. Retrieved from www.ejpmr.com
Search in Google Scholar Back to article
Chang, C.-C., Yang, M.-H., Wen, H.-M., & Chern, J.-C. (2002). Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis, 10(3), 178–182.
Search in Google Scholar Back to article
Clifford, T., Howatson, G., West, D. J., & Stevenson, E. J. (2015). The Potential Benefits of Red Beetroot Supplementation in Health and Disease. Nutrients, 7, 2801–2822. https://doi.org/10.3390/nu7042801
Search in Google Scholar Back to article
de Oliveira, S. P. A., do Nascimento, H. M. A., Sampaio, K. B., & de Souza, E. L. (2020). A review on bioactive compounds of beet (Beta vulgaris L. subsp. vulgaris) with special emphasis on their beneficial effects on gut microbiota and gastrointestinal health. Critical Reviews in Food Science and Nutrition, 1–12. https://doi.org/10.1080/10408398.2020.1768510
Search in Google Scholar Back to article
Eloff, J. N. (1998). Which extractant should be used for the screening and isolation of antimicrobial components from plants? Journal of Ethnopharmacology, 60(1), 1–8. https://doi.org/10.1016/S0378-8741(97)00123-2
Search in Google Scholar Back to article
Evans, W. C. (2009). Trease and Evans Pharmacognosy (16th ed.). Toronto: Elsevier.
Search in Google Scholar Back to article
Gbadamosi, I. T., Moody, J. O., & Yekini, A. O. (2012). Nutritional Composition of Ten Ethnobotanicals Used for the Treatment of Anaemia in Southwest Nigeria. European Journal of Medicinal Plants, 2(2), 140–150. https://doi.org/10.9734/ejmp/2012/1025
Search in Google Scholar Back to article
Hadipour, E., Taleghani, A., Tayarani-Najaran, N., & Tayarani-Najaran, Z. (2020). Biological effects of red beetroot and betalains: A review. Phytotherapy Research, 34(8), 1847–1867. https://doi.org/10.1002/ptr.6653
Search in Google Scholar Back to article
Halwani, A. F., Sindi, H. A., & Jambi, H. A. (2018). Characterization of physical properties of red beet pigments. Journal of Biochemical Technology, 9(2), 16–20.
Search in Google Scholar Back to article
Hanani, E. (2015). Analisis Fitokimia (In Bahasa). Jakarta: Buku Kedokteran EGC.
Search in Google Scholar Back to article
Harborne, J. B. (1987). Metode Fitokimia: Penentuan Cara Modern Menganalisis Tumbuhan (In Bahasa) (K. Padmawinata & I. Soediro, Eds.). Bandung: ITB Press.
Search in Google Scholar Back to article
Heinrich, M., Barnes, J., Gibbons, S., & Williamson, E. M. (2012). Fundamentals of Pharmacognosy and Phytotherapy (2nd Ed.). London: Elsevier.
Search in Google Scholar Back to article
Houghton, P. J., & Raman, A. (1998). Laboratory Handbook for the Fractionation of Natural Extracts. London: Springer Sciences Bussines Media.
Search in Google Scholar Back to article
Huaman-Castilla, N. L., Martínez-Cifuentes, M., Camilo, C., Pedreschi, F., Mariotti-Celis, M., & Pérez-Correa, J. R. (2019). The impact of temperature and ethanol concentration on the global recovery of specific polyphenols in an integrated HPLE/RP process on Carménère pomace extracts. Molecules, 24(17), 1–16. https://doi.org/10.3390/molecules24173145
Search in Google Scholar Back to article
Jaiswal, A., Ganeshpurkar, A., Awasthi, A., Bansal, D., & Dubey, N. (2014). Protective effects of beetroot extract against phenyl hydrazine induced anemia in rats. Pharmacognosy Journal, 6(5), 1–4. https://doi.org/10.5530/pj.2014.5.1
Search in Google Scholar Back to article
Katritzky, A. R., Fara, D. C., Yang, H., Tämm, K., Tamm, T., & Karelson, M. (2004). Quantitative Measures of Solvent Polarity. Chemical Reviews, 104(1), 175–198. https://doi.org/10.1021/cr020750m
Search in Google Scholar Back to article
Khoddami, A., Wilkes, M. A., & Roberts, T. H. (2013). Techniques for analysis of plant phenolic compounds. Molecules, 18(2), 2328–2375.
Search in Google Scholar Back to article
Lesjak, M., & Srai, S. K. S. (2019). Role of dietary flavonoids in iron homeostasis. Pharmaceuticals, 12(119), 1–21. https://doi.org/10.3390/ph12030119
Search in Google Scholar Back to article
Mazhar, M., Faizi, S., Gul, A., Kabir, N., & Simjee, S. U. (2017). Effects of naturally occurring flavonoids on ferroportin expression in the spleen in iron deficiency anemia: In vivo. RSC Advances, 7(38), 23238–23245.
Search in Google Scholar Back to article
Ministry of Health Republic of Indonesia. (2008). Farmakope Herbal Indonesia (Indonesian Herb Pharmacopoeia) (1st Ed.). Jakarta: Ministry of Health Republic of Indonesia.
Search in Google Scholar Back to article
Neha, P., Jain, S. K., Jain, N. K., Jain, H. K., & Mittal, H. K. (2018). Chemical and functional properties of Beetroot (Beta vulgaris L.) for product development: A review. International Journal of Chemical Studies, 6(3), 3190–3194.
Search in Google Scholar Back to article
Nijveldt, R. J., van Nood, E., van Hoorn, D. E. C., Boelens, P. G., van Norren, K., & van Leeuwen, P. A. M. (2001). Flavonoids: A review of probable mechanisms of action and potential applications. The American Journal of Clinical Nutrition, 74, 418–425.
Search in Google Scholar Back to article
Ninfali, P., Antonini, E., Frati, A., & Scarpa, E. (2017). C-Glycosyl Flavonoids from Beta vulgaris Cicla and Betalains from Beta vulgaris rubra: Antioxidant, Anticancer and Antiinflammatory Activities-A Review. Phytotherapy Research, 31(6), 871–884. https://doi.org/10.1002/ptr.5819
Search in Google Scholar Back to article
Odoh, U. E., & Okoro, E. C. (2013). Quantitative phytochemical, proximate/nutritive composition analysis of beta vulgaris linnaeus (chenopodiaceae). International Jpurnal of Current Research, 5(12), 3723–3728.
Search in Google Scholar Back to article
Ologundudu, A., Ologundudu, A. O., Ololade, I. A., & Obi, F. O. (2009). The effect of hibiscus anthocyanins on 2, 4-dinitrophenylhydrazine-induced hepatotoxicity in rabbits. African Journal of Biochemistry Research, 3(4), 140–144.
Search in Google Scholar Back to article
Saeker, S. D., Latif, Z., & Gray, A. L. (Eds.). (2006). Methods in Biotechnology: Natural Products Isolation (2nd ed.). Totowa, New Jersey: Humana Press.
Search in Google Scholar Back to article
Santos-Buelga, C., & Scalbert, A. (2000). Proanthocyanidins and tannin-like compounds – nature, occurrence, dietary intake and effects on nutrition and health. Journal of the Science of Food and Agriculture, 80, 1094–1117. https://doi.org/https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<1094::AIDJSFA569>3.0.CO;2-1
Search in Google Scholar Back to article
Shah, T., Warsi, J., & Laghari, Z. (2020). Tea drinking and its cooccurrence with anemia in pregnant females. Rawal Medical Journal, 45(1), 163–167.
Search in Google Scholar Back to article
Singh et al. (2014). Molecular Mechanism of Phenylhydrazine Induced Haematotoxicity : A Review. Molecular Mechanism of Phenylhydrazine Induced Haematotoxicity : A Review, 2(3), 390–394.
Search in Google Scholar Back to article
Snyder, L. R. (1974). Classification of the solvent properties of common liquids. Journal of Chromatography, 92, 223–230.
Search in Google Scholar Back to article
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, 1(1), 98–106.
Search in Google Scholar Back to article
Wakeel, A., Jan, S. A., Ullah, I., Shinwari, Z. K., & Xu, M. (2019). Solvent polarity mediates phytochemical yield and antioxidant capacity of Isatis tinctoria. PeerJ, 7(e7857), 1–19. https://doi.org/10.7717/peerj.7857
Search in Google Scholar Back to article
Widiyanti, P., Prajogo, B., & Hikmawanti, N. P. E. (2016). Cytotoxicity of Justicia Gendarussa Burm F. Leaf Extracts on Molt-4 Cell. Indonesian Journal of Tropical and Infectious Disease, 6(1), 24–28.
Search in Google Scholar Back to article
Wink, M. (Ed.). (2010). Biochemistry of Plant Secondary Metabolism (2nd Ed.). New Delhi, India: Wiley-Black Well.
Search in Google Scholar Back to article
World Health Organization. (2011). Quality control methods for herbal materials. Retrieved from https://apps.who.int/iris/handle/10665/44479
Search in Google Scholar Back to article
World Health Organization. (2020). Anaemia. Retrieved from Anaemia website: https://www.who.int/health-topics/anaemia#tab=tab_1
Search in Google Scholar Back to article

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

Abstract

Aim

Methods

Results

Conclusion

Paradigm

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