Figure 1.
![The number of mesophilic bacteria [cfu/m3] in different sampling points (average value from all STPs with SD)](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470714071e4585e08a9d3c5/j_acee-2022-0025_fig_001.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=AKIA6AP2G7AKOUXAVR44%2F20251106%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20251106T025941Z&X-Amz-Expires=3600&X-Amz-Signature=0a3a048c17147bcf16ab18a4e21662e1f5cf20bc66c0e0beeb4a4d50552284c0&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 2.
![The number of psychrophilic bacteria [cfu/m3] in different sampling points (average value from 5 STPs with SD)](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470714071e4585e08a9d3c5/j_acee-2022-0025_fig_002.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=AKIA6AP2G7AKOUXAVR44%2F20251106%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20251106T025941Z&X-Amz-Expires=3600&X-Amz-Signature=957b1dfe5e40a30c865e5441f6769737211b2ade47d80d44b6093a9e1678da1c&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 3.
![Contribution of pigmented bacteria [%] in the sum of mesophilic and psychrophilic bacteria in different sampling points (average value from 5 STPs)](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470714071e4585e08a9d3c5/j_acee-2022-0025_fig_003.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=AKIA6AP2G7AKOUXAVR44%2F20251106%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20251106T025941Z&X-Amz-Expires=3600&X-Amz-Signature=6cb2c1dc52a9bc91201ccce359a4c5f7169c009a312c0b157d292f2894676d50&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 4.
![The number of Escherichia coli and Pseudomonas fluorescens [cfu/m3] in different sampling points (average value from 5 STPs with SD)value from 5 STPs)](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470714071e4585e08a9d3c5/j_acee-2022-0025_fig_004.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=AKIA6AP2G7AKOUXAVR44%2F20251106%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20251106T025941Z&X-Amz-Expires=3600&X-Amz-Signature=125a03c2980f6ddad984c70ed54f8ff669201b1cd2d6eae354d57a1b0a57916e&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Figure 5.
![The number of mold fungi [cfu/m3] in different sampling points (average value from 5 STPs with SD)SD)value from 5 STPs)](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470714071e4585e08a9d3c5/j_acee-2022-0025_fig_005.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Content-Sha256=UNSIGNED-PAYLOAD&X-Amz-Credential=AKIA6AP2G7AKOUXAVR44%2F20251106%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Date=20251106T025941Z&X-Amz-Expires=3600&X-Amz-Signature=5c5da5d6606722625364991062d99bbe2ae41049fb4d70b18554804272771358&X-Amz-SignedHeaders=host&x-amz-checksum-mode=ENABLED&x-id=GetObject)
Microbiological air analysis – growth media and culture conditions
| Detected microorganisms | Growth media | Incubation temperature [°C] | Duration of incubation [h] |
|---|---|---|---|
| Mesophiles | nutrient agar (BTL) | 37 | 24-48 |
| Psychrophiles | nutrient agar (BTL) | 26 | 48-96 |
| pigmented bacteria (counted on psychrophiles plates) | nutrient agar (BTL) | 26 | 48-96 |
| Escherichia coli | selective agar Endo (BTL) | 37 | 24-48 |
| Pseudomonas fluorescens | selective Pseudomonas Agar Base with supl. (Oxoid), | 35 | 24-48 |
| Streptococcus faecalis | SF agar (Difco) | 45 | 24-48 |
| mold fungi | Czapek agar (BTL) | 26 | 72-96 |
The list of used abbreviations of monitored technological objects and background
| Abbreviation | Technological object |
|---|---|
| TS | transfer stations |
| G | grits |
| PC | primary clarifiers (open) |
| ASC | activated sludge chambers (open with turbin system of aeration) |
| SC | secondary clarifiers (open) |
| OFC | open fermentation chambers |
| STT | sludge thickening tanks |
| SP | sedimentary plots |
| WW | windward |
| LW | leeward |
The number of different groups of bacteria (range min-max in CFU/m3)_ M – mesophiles; P – psychrophiles, PB – pigmented bacteria; E – E_ coli; Ps – P_ fluorescens; F – mold fungi (the highest microorganisms number are marked as grey cells)
| Sampling point | Group of microorganism | |||||
|---|---|---|---|---|---|---|
| M | P | PB | E | Ps | F | |
| TS 10 | 393-505 | 428-882 | 445-577 | 0-7 | 0-4 | 806-907 |
| TS15 | 66-302 | 459-604 | 223-236 | 0 | 0 | 486-1181 |
| G4 | 229-865 | 446-1323 | 347-812 | 0-20 | 0-9 | 727-786 |
| G45 | 118-222 | 530-1017 | 172-797 | 0 | 0 | 446-826 |
| G50 | 66-83 | 144-511 | 39-289 | 0 | 0 | 275-1284 |
| PC10 | 255-340 | 626-711 | 226-379 | 0-11 | 0-6 | 4468-5459 |
| PC40 | 26-511 | 157-301 | 92-249 | 0 | 0 | 786-1088 |
| PC45 | 59-88 | 164-492 | 59-364 | 0 | 0 | 275-1284 |
| ASC10 | 498-806 | 842-1296 | 341-629 | 6-22 | 7-16 | 917-1127 |
| ASC20 | 197-368 | 276-722 | 118-223 | 0-12 | 0-6 | 197-682 |
| ASC25 | 149-265 | 382-828 | 170-181 | 11-42 | 11-12 | 961-2166 |
| ASC35 | 13-92 | 105-393 | 26-249 | 0-4 | 0-2 | 459-1808 |
| ASC75 | 66-83 | 144-511 | 39-289 | 0 | 0 | 275-1284 |
| SC10 | 119-361 | 316-733 | 175-293 | 6-19 | 6-8 | 2002-2663 |
| SC20 | 149-265 | 382-828 | 149-265 | 0-8 | 0 | 1561-2166 |
| SC45 | 66-141 | 214-572 | 92-183 | 0-4 | 0 | 1023-1982 |
| OFC5 | 85-212 | 159-828 | 85-212 | 4-12 | 0-4 | 1465-2930 |
| OFC10 | 32-43 | 181-361 | 96-127 | 0 | 0 | 892-1051 |
| STT5 | 13-92 | 105-393 | 26-249 | 0 | 0 | 459-1808 |
| STT10 | 182-1127 | 223-2188 | 118-996 | 0-8 | 0 | 262-1638 |
| SP5 | 74-191 | 149-417 | 64-117 | 0 | 0 | 4768-5579 |
| SP15 | 131-276 | 394-983 | 131-353 | 0 | 0 | 381-1037 |
| SP20 | 197-223 | 321-747 | 249-524 | 0 | 0 | 472-708 |
| SP25 | 170-262 | 328-485 | 282-445 | 0-7 | 0 | 550-1212 |
| WW | 32-117 | 255-276 | 195-227 | 0 | 0 | 606-1280 |
| LW | 83-196 | 414-828 | 106-210 | 0 | 0 | 1019-2497 |