Table 1
Oligodeoxynucleotides designed to detect the expression levels of the studied genes
| GENE NAME | 5′ → 3′ SEQUENCE OF THE FORWARD PRIMER | 5′ → 3′ SEQUENCE OF THE FORWARD PRIMER | PRODUCT SIZE (BP) |
|---|---|---|---|
| JHDM1D | TCCCTTCACCTACATTTTCTG | TGCCTGCCTCGCCACATC | 89 |
| PHF2 | CCATCGGTGCCAAGACAGG | GGGGAGGTGGTGTTAGGAG | 164 |
| PHF8 | CCCTCGCCATCATTCACTG | TTCTCCCTCTTCCCGCTGT | 146 |
Table 2
Thermal profile of RT-qPCRs
| STEP | TEMPERATURE [°C] | TIME [MIN] | NUMBER OF CYCLES | |
|---|---|---|---|---|
| Preincubation | 95 | 600 | 1 | |
| Amplification | Denaturation | 95 | 15 | 40 |
| Annealing | 58 | 15 | ||
| Elongation | 72 | 15 | ||
| Melting | 95 | 60 | 1 | |
| 40 | 60 | |||
| 70 | 1 | |||
| 95 | 1 | |||
| Cooling | 37 | 30 | 1 | |
Figure 1
Relative normalized expression of JHDM1D, PHF2 and PHF8 in six time points of the human granulosa cell culture (48h, 72h, 96h, 120h, 144h and 168h). The values are presented as logarithm to the base 2 of the fold change (log(2) FC) of the tested culture duration vs. 24 h culture duration