Figure 1
Oligodeoxynucleotides designed to detect the expression levels of the studied genes
| GENE NAME | 5′ → 3′ SEQUENCE OF THE FORWARD PRIMER | 5′ → 3′ SEQUENCE OF THE FORWARD PRIMER | PRODUCT SIZE (BP) |
|---|---|---|---|
| JHDM1D | TCCCTTCACCTACATTTTCTG | TGCCTGCCTCGCCACATC | 89 |
| PHF2 | CCATCGGTGCCAAGACAGG | GGGGAGGTGGTGTTAGGAG | 164 |
| PHF8 | CCCTCGCCATCATTCACTG | TTCTCCCTCTTCCCGCTGT | 146 |
Thermal profile of RT-qPCRs
| STEP | TEMPERATURE [°C] | TIME [MIN] | NUMBER OF CYCLES | |
|---|---|---|---|---|
| Preincubation | 95 | 600 | 1 | |
| Amplification | Denaturation | 95 | 15 | 40 |
| Annealing | 58 | 15 | ||
| Elongation | 72 | 15 | ||
| Melting | 95 | 60 | 1 | |
| 40 | 60 | |||
| 70 | 1 | |||
| 95 | 1 | |||
| Cooling | 37 | 30 | 1 | |