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Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells Cover

Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Medical Journal of Cell Biology
Volume 8 (2020): Issue 4 (December 2020)
By: Ievgeniia Kocherova,  Katarzyna Stefańska,  Rut Bryl,  Joanna Perek,  Wojciech Pieńkowski,  Jana Zakova,  Igor Crha,  Pavel Ventruba,  Paul Mozdziak and  Michal Ješeta  
Open Access
|Dec 2020

Figures & Tables

Figure 1

BCL2 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
BCL2 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 2

BAX gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
BAX gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 3

P53 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
P53 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Figure 4

CASP9 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001
CASP9 gene expression levels in GCs primary culture. Results are shown as mean ±SD. *p <0.05, ** p <0.01, *** p < 0.001

Sequences of primers used in RT‐PCR for analysis of gene expression

GENEPRIMER SEQUENCE
BCL2F:ATGTGTGTGGAGAGCGTCAA
R:GAAATCAAACAGAGGCCGCA
BAXF:TGACATGTTTTCTGACGGCA
R:CACCCTGGTCTTGGATCCA
p53F:GCTGAATGAGGCCTTGGAAC
R:TTATGGCGGGAGGTAGACTG
CASP9F:TGATGTCGGTGCTCTTGAGA
R:CGCAACTTCTCACAGTCGAT
ACTBF:AAAGACCTGTACGCCAACAC
R:CTCAGGAGGAGCAATGATCTTG
DOI: https://doi.org/10.2478/acb-2020-0023 | Journal eISSN: 2544-3577
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Language: English
Page range: 176 - 182
Submitted on: Nov 4, 2020
|
Accepted on: Dec 15, 2020
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Published on: Dec 31, 2020
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
apoptosis-related genes,
granulosa cells,
primary culture
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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