Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Ievgeniia Kocherova; Katarzyna Stefańska; Rut Bryl; Joanna Perek; Wojciech Pieńkowski; Jana Zakova; Igor Crha; Pavel Ventruba; Paul Mozdziak; Michal Ješeta

doi:10.2478/acb-2020-0023

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Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Medical Journal of Cell Biology

Volume 8 (2020): Issue 4 (December 2020)

By: Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak and Michal Ješeta

Open Access

|Dec 2020

Abstract

Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.

Running title: Apoptosis-related genes expression in granulosa cells in vitro

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Articles in this issue

DOI: https://doi.org/10.2478/acb-2020-0023 | Journal eISSN: 2544-3577

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Language: English

Page range: 176 - 182

Submitted on: Nov 4, 2020

Accepted on: Dec 15, 2020

Published on: Dec 31, 2020

Published by: Foundation for Cell Biology and Molecular Biology

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

apoptosis-related genes,

Related subjects:

© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 8 (2020): Issue 4 (December 2020)