Table 1
The sequences of primers used in the analysis
| GENE NAME | FORWARD PRIMER | REVERSE PRIMER |
|---|---|---|
| CD105 | CTCAGGTCCCCAATGCTACC | GGTTGAAGGCCAGGTAGAGT |
| CD73 | CCCATTGACGAACGGAACAA | TATACCACGTGAATTCCGCC |
| CD14 | CACTAGAGCCCTGCGAAGTA | CGACGGCAATCATACACTGG |
| CD34 | ATGAGACCTCCAGCTGTGAG | AGGTCAGACTGGTGCTTTCT |
| CD90 | CGAGAATGCTACCACCTTGC | AGCCGGAGTTCACATGTGTA |
| CD45 | ACCTAGGCAAACATGTGAGGA | CTTCCAGATCAAAATTTCCACGA |
| HPRT | CCATCACATCGTAGCCCTC | ACTTTTATATCGCCCGTTGAC |
| ACTB | CCCTTGCCGCTCCGCCTTC | GCAGCAATATCGGTCATCCAT |
Figure 1
The results of the RT-qPCR analysis of the change in expression of ASC specific markers before and after osteogenic differentiation. All data was presented as a log2 of fold change