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The influence of osteogenic differentiation on the stem-like properties of adipose derived stem cells – an RT-qPCR study Cover

The influence of osteogenic differentiation on the stem-like properties of adipose derived stem cells – an RT-qPCR study

Medical Journal of Cell Biology
Volume 8 (2020): Issue 4 (December 2020)
By: Rut Bryl,  Claudia Dompe,  Maurycy Jankowski,  Katarzyna Stefańska,  Afsaneh Golkar Narenji,  Jakub Kulus,  Magdalena Kulus,  Maria Wieczorkiewicz,  Grzegorz Wąsiatycz,  Jędrzej M. Jaśkowski,  Mariusz Kaczmarek,  James N. Petitte,  Paul Mozdziak,  Paweł Antosik and  Dorota Bukowska  
Open Access
|Dec 2020

Figures & Tables

Figure 1

The results of the RT-qPCR analysis of the change in expression of ASC specific markers before and after osteogenic differentiation. All data was presented as a log2 of fold change

The sequences of primers used in the analysis

GENE NAMEFORWARD PRIMERREVERSE PRIMER
CD105CTCAGGTCCCCAATGCTACCGGTTGAAGGCCAGGTAGAGT
CD73CCCATTGACGAACGGAACAATATACCACGTGAATTCCGCC
CD14CACTAGAGCCCTGCGAAGTACGACGGCAATCATACACTGG
CD34ATGAGACCTCCAGCTGTGAGAGGTCAGACTGGTGCTTTCT
CD90CGAGAATGCTACCACCTTGCAGCCGGAGTTCACATGTGTA
CD45ACCTAGGCAAACATGTGAGGACTTCCAGATCAAAATTTCCACGA
HPRTCCATCACATCGTAGCCCTCACTTTTATATCGCCCGTTGAC
ACTBCCCTTGCCGCTCCGCCTTCGCAGCAATATCGGTCATCCAT
DOI: https://doi.org/10.2478/acb-2020-0020 | Journal eISSN: 2544-3577
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Language: English
Page range: 158 - 163
Submitted on: Oct 21, 2020
|
Accepted on: Dec 3, 2020
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Published on: Dec 31, 2020
Published by: Foundation for Cell Biology and Molecular Biology
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
adipose,
stem cells,
differentiation,
osteogenic,
markers
Related subjects:
Life sciences,
Molecular biology,
Biochemistry

© 2020 Rut Bryl, Claudia Dompe, Maurycy Jankowski, Katarzyna Stefańska, Afsaneh Golkar Narenji, Jakub Kulus, Magdalena Kulus, Maria Wieczorkiewicz, Grzegorz Wąsiatycz, Jędrzej M. Jaśkowski, Mariusz Kaczmarek, James N. Petitte, Paul Mozdziak, Paweł Antosik, Dorota Bukowska, published by Foundation for Cell Biology and Molecular Biology
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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