‘Cell cycle’ and ‘cell death’- related genes are differentially expressed during long – term in vitro real-time cultivation of porcine oviductal epithelial cells

Kulus, Magdalena; Kulus, Jakub; Popis, Małgorzata; Borowiec, Blanka; Stefańska, Katarzyna; Celichowski, Piotr; Nawrocki, Mariusz J.; Brüssow, Klaus P.; Kempisty, Bartosz; Jeseta, Michal; Antosik, Paweł; Bukowska, Dorota

doi:10.2478/acb-2019-0012

Abstract

Alterations in cells depend on their genetic material, its activation and translation of the products. The genes responsible for the cell cycle processes and apoptosis of porcine oviductal cells have been presented in our study. The processes occurring in the reproductive system of females are extremely complex and require in-depth knowledge. Thanks to in vitro studies on the fallopian tube epithelium cells, we can get closer to understanding the biochemical and morphological changes occurring in mammalian organisms. Our research was conducted on fallopian tubes obtained from commercially bred pigs and its aim was to assess the expression profile of genes responsible for the most important processes of cellular life. Cell cultures were carried out for 30 days, with the obtained cells subjected to molecular analysis. We have shown significant regulation of “cell death” and “cell cycle” genes, some of which are related to the reproductive system. The alterations in transcriptomic profile and mutual relations between the genes were analyzed and related to the literature findings. The knowledge gained could help in identifying new potential markers of the in vitro occurrence of processes described by the ontology groups of interest.

Running title: pig, oocytes, microarray assays, in vitro maturation (IVM)

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‘Cell cycle’ and ‘cell death’- related genes are differentially expressed during long – term in vitro real-time cultivation of porcine oviductal epithelial cells

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