Have a personal or library account? Click to login
Isolation and characterization of endophytic bacteria from tomato foliage and their in vitro efficacy against root-knot nematodes Cover

Isolation and characterization of endophytic bacteria from tomato foliage and their in vitro efficacy against root-knot nematodes

Journal of Nematology
Volume 53 (2021): Issue 1 (January 2021)
By: Binita Basumatary,  Debanand Das,  B. N. Choudhury,  Pranab Dutta and  Ashok Bhattacharyya  
Open Access
|Dec 2021

Figures & Tables

Figure 1:

Growth of endophytic bacteria from cut pieces of stem and leaf on NA media. Solanum lycopersicum stem Solanum lycopersicum leave Solanum pimpinellifolium leave.
Growth of endophytic bacteria from cut pieces of stem and leaf on NA media. Solanum lycopersicum stem Solanum lycopersicum leave Solanum pimpinellifolium leave.

Figure S1:

Scanning Electron Micrograph of isolate EBTL1 (Microbacterium arborescens).
Scanning Electron Micrograph of isolate EBTL1 (Microbacterium arborescens).

Figure S2:

Scanning Electron Micrograph of isolate BETL2 (Bacillus marisflavi).
Scanning Electron Micrograph of isolate BETL2 (Bacillus marisflavi).

Figure S3:

Scanning Electron Micrograph of isolate BETL4 (Bacillus altitudinis).
Scanning Electron Micrograph of isolate BETL4 (Bacillus altitudinis).

Figure S4:

Scanning Electron Micrograph of isolate BETS2 (Exiguobacterium indicum).
Scanning Electron Micrograph of isolate BETS2 (Exiguobacterium indicum).

Figure 2:

Phylogenetic tree showing the genetic relationship of the BETL1 isolate to other isolates by using maximum likelihood method.
Phylogenetic tree showing the genetic relationship of the BETL1 isolate to other isolates by using maximum likelihood method.

Figure 3:

Phylogenetic tree showing the genetic relationship of the BETL2 isolate to other isolates by using maximum likelihood method.
Phylogenetic tree showing the genetic relationship of the BETL2 isolate to other isolates by using maximum likelihood method.

Figure 4:

Phylogenetic tree showing the genetic relationship of the BETL4 isolate to other isolates by using maximum likelihood method.
Phylogenetic tree showing the genetic relationship of the BETL4 isolate to other isolates by using maximum likelihood method.

Figure 5:

Phylogenetic tree showing the genetic relationship of the BETS2 isolate to other isolates by using maximum likelihood method.
Phylogenetic tree showing the genetic relationship of the BETS2 isolate to other isolates by using maximum likelihood method.

Figure 6:

Phylogenetic tree showing the genetic relationship of the BETL6 isolate to other isolates by using maximum likelihood method.
Phylogenetic tree showing the genetic relationship of the BETL6 isolate to other isolates by using maximum likelihood method.

Figure 7:

Effect of culture filtrates of isolated bacterial endophytes on juvenile mortality of Meloidogyne incognita at 10% (S/10) concentration at different exposure time.
Effect of culture filtrates of isolated bacterial endophytes on juvenile mortality of Meloidogyne incognita at 10% (S/10) concentration at different exposure time.

Endophytic bacterial isolates from Solanum lycopersicum and Solanum pimpinellifolium_

SourceTissueNo. of isolatesName of isolates
Solanum lycopersicum leaf8BETL1, BETL2, BETL3, BETL4, BETL5, BETL6, BETL7 and BETL8
S. lycopersicum stem2BETS1 and BETS2
Solanum pimpinellifolium (konbilahi)leaf5BEKL1, BEKL2, BEKL3, BEKL4 and BEKL5

Effect of culture filtrate of isolated bacterial endophytes on juvenile mortality of Meloidogyne incognita_

Period of exposure (hrs)
TreatmentCulture filtrate concentration6122448Treatment (T) MeanCulture filtrate concentration (C) Mean
BETL1S/1058.00 (49.61)64.00 (53.18)75.33 (60.23)80.67 (63.96)79.07 (63.64)c 47.24 (42.36)
S/666.67 (54.86)71.33 (57.67)78.00 (62.04)84.00 (66.48)55.78 (47.70)
S/472.00 (58.09)78.67 (62.51)80.00 (63.49)88.67 (70.53)63.09 (52.62)
S/273.33 (61.60)82.00 (64.96)84.67 (66.97)92.00 (73.94)69.79 (57.62)
S81.33 (64.49)86.67 (68.70)88.67 (70.53)95.33 (78.90)76.51 (63.12)
BETL2S/1054.67 (47.68)66.00 (54.34)76.67 (61.16)84.67 (67.08)81.47 (66.74)a
S/660.00 (50.79)72.00 (58.09)80.67 (64.05)90.00 (71.76)
S/464.00 (53.14)78.00 (62.04)85.33 (67.61)94.00 (76.11)
S/278.00 (62.23)85.33 (67.61)91.33 (72.95)100.00 (89.93)
S83.33 (65.99)89.33 (71.06)95.33 (78.90)100.00 (89.93)
BETL3S/108.00 (16.35)22.00 (27.90)44.00 (41.52)60.67 (51.19)49.07 (44.17)m
S/610.67 (18.93)28.00 (31.78)47.33 (43.47)64.00 (53.14)
S/419.33 (26.04)38.00 (37.99)60.67 (51.18)70.67 (57.25)
S/226.00 (30.63)52.67 (46.53)67.33 (55.15)78.00 (62.09)
S50.00 (45.00)67.33 (55.28)80.67 (63.93)86.00 (68.11)
BETS1S/1026.67 (31.04)48.67 (44.23)63.33 (52.75)76.67 (61.23)65.90 (55.13)jk
S/631.33 (33.98)52.00 (46.15)72.00 (58.13)80.00 (63.49)
S/435.33 (36.45)62.67 (52.36)78.00 (62.09)82.67 (65.45)
S/240.00 (39.22)69.33 (56.41)80.00 (63.49)88.67 (70.63)
S67.33 (55.15)80.67 (63.96)89.33 (71.06)93.33 (75.23)
BETL4S/1052.00 (46.15)64.00 (53.14)72.67 (58.50)82.00 (64.96)81.43 (66.62)ab
S/661.33 (51.59)72.67 (58.50)80.00 (63.46)86.00 (68.11)
S/470.00 (56.85)79.33 (63.00)87.33 (69.24)91.33 (72.95)
S/276.67 (61.27)85.33 (67.51)93.33 (75.23)97.33 (82.77)
S86.67 (68.70)91.33 (72.95)100.00 (89.93)100.00 (89.93)
BETL5S/1031.33 (34.01)46.00 (42.69)50.67 (45.38)67.33 (55.21)64.20 (53.72)l
S/640.00 (39.20)57.33 (49.22)66.00 (54.42)70.67 (57.39)
S/446.67 (43.06)66.00 (54.34)72.67 (58.50)77.33 (61.60)
S/248.67 (44.23)72.67 (58.50)78.00 (62.09)82.00 (64.99)
S62.00 (51.96)78.00 (62.14)82.67 (65.45)88.00 (69.93)
BETL6S/1052.00 (46.15)60.67.00 (51.21)72.67 (58.55)80.67 (63.96)78.17 (63.56)de
S/660.67 (51.17)70.00 (56.80)78.00 (62.09)84.00 (66.48)
S/466 (54.39)76.67 (61.16)82.67 (65.45)89.33 (71.06)
S/272.00 (58.14)80.00 (63.46)88.00 (69.93)94.67 (78.21)
S78.67 (62.56)86.00 (68.11)90.67 (72.36)100.00 (89.93)
BEKL1S/1019.33 (25.92)26.00 (30.54)65.33 (53.97)76.67 (61.18)67.20 (56.23)h
S/636.00 (36.84)42.67 (40.78)74.67 (59.83)80.67 (63.99)
S/444.00 (41.52)48.67 (44.23)80.00 (63.53)84.67 (67.08)
S/265.33 (53.97)76.00 (60.69)82.67 (65.58)89.33 (71.06)
S81.33 (64.45)87.33 (69.24)88.67 (70.53)94.67 (79.60)
BETS2S/1042.00 (40.39)66.00 (54.36)70.67 (57.27)83.33 (65.94)78.87 (65.15)cd
S/651.33 (45.76)71.33 (57.68)80.67 (64.05)87.33 (69.50)
S/460.00 (50.78)80.67 (63.96)86.00 (68.11)90.00 (71.76)
S/270.67 (57.27)84.00 (66.48)90.67 (72.36)96.67 (81.88)
S82.67 (65.45)88.67 (70.53)94.67 (78.21)100.00 (89.93)
BEKL2S/1022.67 (28.30)29.33 (32.77)44.00 (41.55)68.67 (55.99)73.33 (60.72)f
S/660.00 (50.80)69.33 (56.45)79.33 (62.99)85.33 (67.57)
S/468.67 (55.99)76.00 (60.74)84.00 (66.48)89.33 (71.42)
S/272.00 (58.09)80.67 (63.96)88.67 (70.47)94.67 (78.21)
S77.33 (61.65)84.00 (66.48)92.67 (74.53)100.00 (89.93)
BETL7S/1018.00 (25.01)30.67 (33.61)51.33 (45.77)70.67 (57.28)66.23 (55.37)j
S/627.33 (31.47)41.33 (39.99)61.33 (51.57)80.67 (64.05)
S/454.00 (47.31)70.00 (56.85)76.67 (61.14)85.33 (67.51)
S/268.00 (55.56)75.33 (60.25)83.33 (65.94)91.33 (72.95)
S76.00 (60.69)81.33 (64.49)87.33 (69.24)94.66 (76.81)
BEKL3S/1014.00 (21.89)33.33 (35.24)57.33 (49.23)73.33 (58.99)67.19 (56.08)hi
S/636.00 (36.86)46.67 (43.08)71.33 (57.67)79.33 (63.15)
S/452.00 (46.15)61.33 (51.61)82.00 (64.99)86.67 (68.70)
S/266.00 (54.34)73.33 (58.94)86.00 (68.37)90.67 (72.36)
S70.67 (57.23)80.00 (63.46)89.33 (71.06)94.67 (78.21)
BEKL4S/1015.33 (22.92)42.67 (40.78)64.67 (53.55)82.00 (64.99)71.33 (59.95)g
S/620.67 (26.94)54.67 (47.68)78.00 (62.09)87.33 (69.24)
S/434.00 (35.61)66.67 (54.79)88.00 (69.93)92.67 (74.53)
S/266.00 (54.36)80.00 (63.46)91.33 (73.24)96.67 (80.67)
S82.67 (65.49)88.67 (70.53)94.67 (78.21)100.00 (89.93)
BETL8S/1016.00 (23.51)22.00 (27.90)28.00 (31.91)36.00 (36.86)37.07 (37.25)o
S/621.33 (27.46)26.00 (30.63)32.67 (34.84)42.67 (40.78)
S/427.33 (31.49)31.33 (34.03)38.00 (38.04)49.33 (44.62)
S/232.67 (34.84)36.67 (37.26)46.67 (43.08)54.00 (47.31)
S40.00 (39.22)46.00 (42.70)52.67 (46.53)62.00 (52.01)
BEKL5S/1020.67 (27.03)25.33 (30.19)30.67 (33.61)35.33 (36.46)39.20 (38.61)n
S/626.67 (31.07)30.67 (33.61)36.00 (36.84)40.00 (39.22)
S/430.00 (33.19)35.33 (36.46)42.67 (40.77)46.67 (43.08)
S/235.33 (36.46)42.00 (40.39)48.67 (44.23)52.67 (46.53)
S41.33 (40.01)49.33 (44.62)55.33 (48.07)59.33 (50.39)
Distilled Water (SDW)S0000(0.00)
S0000
S0000
S0000
S0000
Period of Exposure(t) Mean 46.93 (42.16)58.28 (49.34)68.51 (56.29)76.23 (62.95)

Morphological and biochemical characteristics of the isolated strains of endophytic bacteria_

Isolated strainsColourGrams’ stainingShapeMotilityKOHCitrate utilizationGelatin hydrolysisCatalase testStarch hydrolysis
BETL1Orange+veRod+-ve+ve+ve+ve-ve
BETL2Shiny yellow+veRod+-ve-ve+ve+ve-ve
BETL3Dark yellow-veRod++ve+ve+ve+ve+ve
BETS1Yellow-veRod++ve-ve+ve+ve-ve
BETL4White+veRod+-ve+ve+ve+ve+ve
BETL5Yellow-veDiplococcus++ve-ve+ve+ve-ve
BETL6Shiny yellow+veRod+-ve+ve+ve+ve-ve
BEKL1White-veCoccus++ve+ve+ve+ve-ve
BETS2Yellowish orange+veRod+-ve-ve+ve+ve+ve
BEKL2Cream-veRod++ve+ve+ve+ve+ve
BETL7White-veCoccus++ve+ve+ve+ve+ve
BEKL3Yellow-veDiplococcus++ve+ve+ve+ve-ve
BEKL4Cream-veCoccus++ve+ve+ve+ve-ve
BETL8Cream+veRod+-ve+ve+ve+ve+ve
BEKL5Yellow-veRod++ve+ve+ve+ve-ve
DOI: https://doi.org/10.21307/jofnem-2021-104 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Journal RSS Feed
Language: English
Page range: 1 - 16
Submitted on: Apr 28, 2021
Published on: Dec 21, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Culture filtrate,
Efficacy,
Endophytic bacteria,
Exposure time,
Juvenile mortality,
Meloidogyne incognita,
Root-knot nematode,
16S rRNA
Related subjects:
Life sciences,
Life sciences, other

© 2021 Binita Basumatary, Debanand Das, B. N. Choudhury, Pranab Dutta, Ashok Bhattacharyya, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous articleVolume 53 (2021): Issue 1 (January 2021)Next article