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First report of root-lesion nematode, Pratylenchus oleae from pistachio in Iran Cover

First report of root-lesion nematode, Pratylenchus oleae from pistachio in Iran

Open Access
|Dec 2021

Figures & Tables

Figure 1:

Iranian isolate of Pratylenchus oleae. A and B: Anterior region; C: Lateral field at mid-body; D: Genital tract and vulva region; E-G: Variation in tail shape, (H) Adult female.
Iranian isolate of Pratylenchus oleae. A and B: Anterior region; C: Lateral field at mid-body; D: Genital tract and vulva region; E-G: Variation in tail shape, (H) Adult female.

Figure 2:

The gel with PCR amplicons obtained with D2–D3 expansion segments of 28S rRNA sequences (Line 1) and species specific primers for Pratylenchus oleae (Line 2). M – 100 bp DNA Marker.
The gel with PCR amplicons obtained with D2–D3 expansion segments of 28S rRNA sequences (Line 1) and species specific primers for Pratylenchus oleae (Line 2). M – 100 bp DNA Marker.

Figure 3:

The Bayesian tree inferred from known and newly sequenced Pratylenchus oleae from IRAN based on the 28S rDNA region under GTR + I + G model.
The Bayesian tree inferred from known and newly sequenced Pratylenchus oleae from IRAN based on the 28S rDNA region under GTR + I + G model.

Phylogenetic characters of Iranian isolate of Pratylenchus oleae (MW338666) compared to others isolates_

SpeciesGenebank Accession/28S-rDNASimilarityQuery CoverNucleotide DifferencesGap
Pratylenchus oleae KJ51085599%79%20
Pratylenchus oleae KJ51085699%100%71
Pratylenchus oleae KJ51085799%100%71
Pratylenchus oleae KJ51085899%98%51
Pratylenchus oleae KJ51085999%100%72
Pratylenchus oleae KJ51086099%84%62
Pratylenchus oleae KJ510861100%79%20
Pratylenchus capsici MH796977100%94%313
Pratylenchus capsici MH796976100%94%323
Pratylenchus capsici MH796970100%93%374
Pratylenchus capsici MH796975100%95%314
Pratylenchus capsici MH796978100%94%334
Pratylenchus capsici MH796974100%93%349
Pratylenchus capsici MH796969100%93%329
Pratylenchus capsici MH796971100%93%306
Pratylenchus capsici MH796973100%94%336
Pratylenchus dunensis AJ89045989%99%616
Pratylenchus penetrans JX26196187%100%8213

Morphometric characters of Iranian isolate of Pratylenchus oleae on different isolates_

CharacterYazd – Ardakan (Iran)Agua Amarga-Níjar (Spain)Albaricoques-Níjar (Spain)Ouled Chamekh (Tunisia)
n1020814
L522.5 ± 34.9 (463.0–565.0)455 ± 30.4 (412–511)464 ± 31.6 (416–513)501 ± 37.8 (440–555)
a28.3 ± 1.6 (25.7–31.0)24.4 ± 1.8 (21.0–27.8)25.1 ± 1.8 (22.5–27.0)28.1 ± 1.4 (26.9–32.6)
b5.9 ± 0.4 (5.2–6.7)4.5 ± 0.3 (4.0–5.4)4.6 ± 0.3 (4.0–5.2)5.0 ± 0.6 (4.3–5.9)
4.2 ± 0.3 (3.6–4.7)---
c20.2 ± 0.8 (19.1–21.0)20.2 ± 1.8 (17.0–24.3)19.1 ± 1.0 (17.0–20.1)22.1 ± 2.1 (19.0–25.2)
2.1 ± 0.1 (1.8–2.3)2.0 ± 0.2 (1.7–2.4)2.2 ± 0.1 (2.0–2.4)1.9 ± 0.3 (1.7–2.3)
V80.8 ± 1.5 (77.8–83.0)80.1 ± 1.3 (78.0–82.0)80.2 ± 1.3 (78.0–82.0)79.3 ± 1.5 (78.5–81.5)
Stylet Length17.3 ± 0.7 (16.0–18.0)16.5 ± 0.6 (14.5–17.0)16.4 ± 0.7 (15.0–17.0)15.8 ± 0.6 (15.0–17.0)
Median Bulb55.6 ± 2.3 (51.0–59.0)46.4 ± 1.7 (44.0–51.0)46.3 ± 1.6 (44.9–49.6)47.6 ± 3.0 (42.0–50.9)
Anterior to Excretory Pore88.9 ± 2.7 (85.0–93.0)82.0 ± 5.3 (71.0–95.0)82.4 ± 5.8 (71.0–91.0)78.0 ± 5.2 (73.0–87.0)
Anterior End to Pharyngeal Junction89.0 ± 4.6 (78.0–94.0)---
Pharynx Length123.5 ± 3.4 (118.0–129.0)102.0 ± 7.6 (90.0–113.0)100.1 ± 4.3 (92.0–106.0)96.8 ± 7.1 (89.0–110.0)
Max. Body Diam.18.5 ± 1.0 (17.0–20.0)---
Body Diam. at Anus12.6 ± 0.5 (12.0–13.0)---
Tail Length25.9 ± 1.7 (22.0–27.0)22.7 ± 2.2 (19.0–26.5)24.4 ± 1.7 (21.0–27.0)23.1 ± 3.1 (19.0–29.0)
DOI: https://doi.org/10.21307/jofnem-2021-103 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 7
Submitted on: Aug 31, 2021
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Published on: Dec 8, 2021
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2021 Farhad Saeidi Naeini, Zahra Majd Taheri, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.