Figure 1:
Line drawings of Basilaphelenchus brevistylus n. sp. (A) female; (B) male; (C) female anterior region; (D) vulval region; (E) female tail; (F) male tail; (G) spicule. (Scale bars: A, B = 50 µm; C-G = 10 µm).
Figure 2:
Females of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) anterior region; (D) metacorpus; (E) lateral lines; (F) oocytes; (G) vulva region and post-vulval sac; (H) tail. (Scale bars: A = 100 µm; B-H = 10 µm).
Figure 3:
Males of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) testis cells; (D, F) tail and spicule; (E) tail tip; (G) papillae. (Scale bars: A = 100 µm; B-G = 10 µm).
Table 1.
Morphometrics of Basilaphelenchus brevistylus n. sp
| Female | Male | ||
|---|---|---|---|
| Character | Holotype | Paratypes | Paratypes |
| n | – | 15 | 15 |
| L | 469.1 | 413.6 ± 35.4 (364.3-483.1) | 381.7 ± 19.6 (352.4-413.1) |
| a | 29.7 | 29.2 ± 2.9 (25.2-36.8) | 33.6 ± 3.0 (29.6-40.5) |
| b | 10.4 | 9.9 ± 0.5 (9.4-10.9) | 8.9 ± 0.5 (8-9.6) |
| c | 18.5 | 17.7 ± 1.3 (16.2-20.9) | 16.2 ± 1.0 (13.9-17.8) |
| c′ | 3.0 | 3.1 ± 0.3 (2.7-3.5) | 2.9 ± 0.2 (2.6-3.4) |
| V or T | 72.2 | 71.7 ± 1.1 (69.8-73.8) | 49.9 ± 4.6 (43.2-61.7) |
| M | 30.0 | 32.5 ± 3.7 (28.0-38.0) | 31.0 ± 4.4 (24.0-37.0) |
| Max. body diam. | 15.8 | 14.1 ± 1.8 (11.4-18.0) | 11.4 ± 1.1 (9.8-13.2) |
| Lip region diam. | 4.4 | 4.5 ± 0.4 (4.1-5.3) | 4.4 ± 0.2 (4-4.8) |
| Lip region height | 2.5 | 2.4 ± 0.3 (2-2.6) | 2.3 ± 0.1 (2.1-2.5) |
| Stylet conus | 1.5 | 1.6 ± 0.2 (1.4-1.9) | 1.4 ± 0.2 (1.1-1.8) |
| Stylet length (total) | 5.0 | 4.9 ± 0.3 (4.5-5.5) | 4.6 ± 0.4 (4.0-5.0) |
| Distal end of metacorpus from anterior | 45.0 | 41.5 ± 2.5 (37.7-45.1) | 42.6 ± 1.3 (39.0-43.7) |
| Metacorpus length | 9.2 | 8.4 ± 0.6 (7.3-9.5) | 8.3 ± 0.6 (7.3-9.7) |
| Metacorpus diam. | 6.6 | 6.8 ± 0.7 (5.8-8.1) | 6.6 ± 0.5 (5.4-7.8) |
| Position of the valve of metacorpus (%) | 65.2 | 66.0 ± 3.7 (60.7-72) | 67.3 ± 2.7 (63-72) |
| Body diam. at metacorpus level | 10.9 | 11.0 ± 2.4 (9.7-11.7) | 9.6 ± 0.7 (8.5-10.8) |
| Gonad length | 213.3 | 190.1 ± 21.8 (160.1-223.9) | 189.9 ± 18.4 (163.9-242.6) |
| Vulval body diam. | 12.0 | 12.1 ± 1.2 (10.5-14.4) | – |
| Spicule length (chord) | – | – | 11.5 ± 0.7 (10.3-12.8) |
| Spicule length (arc) | – | – | 10.5 ± 0.6 (9.6-11.3) |
| Post-vulval uterine sac length | 48.8 | 45.9 ± 7.0 (36.6-56.5) | – |
| Vulva-anus distance | 104.9 | 93.7 ± 7.1 (83.1-107.3) | – |
| Anal or cloacal body diam. | 8.4 | 7.3 ± 0.8 (6.5-8.9) | 8.0 ± 0.3 (7.5-8.7) |
| Tail length | 25.4 | 22.0 ± 0.6 (20-26.6) | 23.5 ± 1.6 (20.1-27.3) |
Figure 4:
Bayesian consensus tree inferred from D2-D3 under GTR + I + G model (‒lnL = 18,080.8008; freqA = 0.1951; freqC = 0.1764; freqG = 0.3257; freqT = 0.3028; R(a) = 0.8366; R(b) = 2.5599; R(c) = 1.221; R(d) = 0.5867; R(e) = 4.2157; R(f) = 1; Pinva = 0.1777; Shape = 0.9323). Posterior probability values exceeding 50% are given on appropriate clades.
Figure 5:
Bayesian consensus tree inferred from SSU under GTR + I + G model (‒lnL = 27,834.9805; freqA = 0.2254; freqC = 0.1985; freqG = 0.2797; freqT = 0.2964; R(a) = 1.0461; R(b) = 2.3739; R(c) = 1.4729; R(d) = 0.7716; R(e) = 2.9919; R(f) = 1; Pinva = 0.0959; Shape = 0.7961). Posterior probability values exceeding 50% are given on appropriate clades.