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A new aphelench nematode, Basilaphelenchus brevistylus n. sp. (Aphelenchoididae: Tylaphelenchinae) from Pinus massoniana in China Cover

A new aphelench nematode, Basilaphelenchus brevistylus n. sp. (Aphelenchoididae: Tylaphelenchinae) from Pinus massoniana in China

Open Access
|Aug 2021

Figures & Tables

Figure 1:

Line drawings of Basilaphelenchus brevistylus n. sp. (A) female; (B) male; (C) female anterior region; (D) vulval region; (E) female tail; (F) male tail; (G) spicule. (Scale bars: A, B = 50 µm; C-G = 10 µm).
Line drawings of Basilaphelenchus brevistylus n. sp. (A) female; (B) male; (C) female anterior region; (D) vulval region; (E) female tail; (F) male tail; (G) spicule. (Scale bars: A, B = 50 µm; C-G = 10 µm).

Figure 2:

Females of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) anterior region; (D) metacorpus; (E) lateral lines; (F) oocytes; (G) vulva region and post-vulval sac; (H) tail. (Scale bars: A = 100 µm; B-H = 10 µm).
Females of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) anterior region; (D) metacorpus; (E) lateral lines; (F) oocytes; (G) vulva region and post-vulval sac; (H) tail. (Scale bars: A = 100 µm; B-H = 10 µm).

Figure 3:

Males of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) testis cells; (D, F) tail and spicule; (E) tail tip; (G) papillae. (Scale bars: A = 100 µm; B-G = 10 µm).
Males of Basilaphelenchus brevistylus n. sp. under the light microscrope. (A) entire body; (B) lip region and stylet; (C) testis cells; (D, F) tail and spicule; (E) tail tip; (G) papillae. (Scale bars: A = 100 µm; B-G = 10 µm).

Figure 4:

Bayesian consensus tree inferred from D2-D3 under GTR + I + G model (‒lnL = 18,080.8008; freqA = 0.1951; freqC = 0.1764; freqG = 0.3257; freqT = 0.3028; R(a) = 0.8366; R(b) = 2.5599; R(c) = 1.221; R(d) = 0.5867; R(e) = 4.2157; R(f) = 1; Pinva = 0.1777; Shape = 0.9323). Posterior probability values exceeding 50% are given on appropriate clades.
Bayesian consensus tree inferred from D2-D3 under GTR + I + G model (‒lnL = 18,080.8008; freqA = 0.1951; freqC = 0.1764; freqG = 0.3257; freqT = 0.3028; R(a) = 0.8366; R(b) = 2.5599; R(c) = 1.221; R(d) = 0.5867; R(e) = 4.2157; R(f) = 1; Pinva = 0.1777; Shape = 0.9323). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 5:

Bayesian consensus tree inferred from SSU under GTR + I + G model (‒lnL = 27,834.9805; freqA = 0.2254; freqC = 0.1985; freqG = 0.2797; freqT = 0.2964; R(a) = 1.0461; R(b) = 2.3739; R(c) = 1.4729; R(d) = 0.7716; R(e) = 2.9919; R(f) = 1; Pinva = 0.0959; Shape = 0.7961). Posterior probability values exceeding 50% are given on appropriate clades.
Bayesian consensus tree inferred from SSU under GTR + I + G model (‒lnL = 27,834.9805; freqA = 0.2254; freqC = 0.1985; freqG = 0.2797; freqT = 0.2964; R(a) = 1.0461; R(b) = 2.3739; R(c) = 1.4729; R(d) = 0.7716; R(e) = 2.9919; R(f) = 1; Pinva = 0.0959; Shape = 0.7961). Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics of Basilaphelenchus brevistylus n_ sp

FemaleMale
CharacterHolotypeParatypesParatypes
n 1515
L 469.1413.6 ± 35.4 (364.3-483.1)381.7 ± 19.6 (352.4-413.1)
a 29.729.2 ± 2.9 (25.2-36.8)33.6 ± 3.0 (29.6-40.5)
b 10.49.9 ± 0.5 (9.4-10.9)8.9 ± 0.5 (8-9.6)
c 18.517.7 ± 1.3 (16.2-20.9)16.2 ± 1.0 (13.9-17.8)
c′ 3.03.1 ± 0.3 (2.7-3.5)2.9 ± 0.2 (2.6-3.4)
V or T 72.271.7 ± 1.1 (69.8-73.8)49.9 ± 4.6 (43.2-61.7)
M 30.032.5 ± 3.7 (28.0-38.0)31.0 ± 4.4 (24.0-37.0)
Max. body diam.15.814.1 ± 1.8 (11.4-18.0)11.4 ± 1.1 (9.8-13.2)
Lip region diam.4.44.5 ± 0.4 (4.1-5.3)4.4 ± 0.2 (4-4.8)
Lip region height2.52.4 ± 0.3 (2-2.6)2.3 ± 0.1 (2.1-2.5)
Stylet conus1.51.6 ± 0.2 (1.4-1.9)1.4 ± 0.2 (1.1-1.8)
Stylet length (total)5.04.9 ± 0.3 (4.5-5.5)4.6 ± 0.4 (4.0-5.0)
Distal end of metacorpus from anterior45.041.5 ± 2.5 (37.7-45.1)42.6 ± 1.3 (39.0-43.7)
Metacorpus length9.28.4 ± 0.6 (7.3-9.5)8.3 ± 0.6 (7.3-9.7)
Metacorpus diam.6.66.8 ± 0.7 (5.8-8.1)6.6 ± 0.5 (5.4-7.8)
Position of the valve of metacorpus (%)65.266.0 ± 3.7 (60.7-72)67.3 ± 2.7 (63-72)
Body diam. at metacorpus level10.911.0 ± 2.4 (9.7-11.7)9.6 ± 0.7 (8.5-10.8)
Gonad length213.3190.1 ± 21.8 (160.1-223.9)189.9 ± 18.4 (163.9-242.6)
Vulval body diam.12.012.1 ± 1.2 (10.5-14.4)
Spicule length (chord)11.5 ± 0.7 (10.3-12.8)
Spicule length (arc)10.5 ± 0.6 (9.6-11.3)
Post-vulval uterine sac length48.845.9 ± 7.0 (36.6-56.5)
Vulva-anus distance104.993.7 ± 7.1 (83.1-107.3)
Anal or cloacal body diam.8.47.3 ± 0.8 (6.5-8.9)8.0 ± 0.3 (7.5-8.7)
Tail length25.422.0 ± 0.6 (20-26.6)23.5 ± 1.6 (20.1-27.3)
DOI: https://doi.org/10.21307/jofnem-2021-070 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 12
Published on: Aug 5, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2021 Bashiy Akol, Qiuling Huang, Borong Lin, Honghong Wang, Jinling Liao, Kan Zhuo, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.