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Pristionchus trametes n. sp. (Diplogastridae) isolated from the mushroom Trametes orientalis in Kyoto, Japan

Open Access
|Jul 2021

Figures & Tables

Figure 1:

Pristionchus trametes n. sp. adults in the right lateral view. A: Female (ov, ovary; jt, junction tissue; sp, spermatheca; od, oviduct; ut, uterus; v, vulva; pd, postdeirid). B: Male (ref, reflexed part of testis; tes, testis; vd, vas deferens).
Pristionchus trametes n. sp. adults in the right lateral view. A: Female (ov, ovary; jt, junction tissue; sp, spermatheca; od, oviduct; ut, uterus; v, vulva; pd, postdeirid). B: Male (ref, reflexed part of testis; tes, testis; vd, vas deferens).

Figure 2:

Pristionchus trametes n. sp. adults. A: Surface of the lip region. B, C: Stomatal region. D: Anterior part of an adult. E: Vulval region. F, G: Female tail (ph, phasmid). H: Male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; ph, phasmid). I: Male tail tip (labels are same as H). J: Spicule and gubernaculum. A and B, left lateral view; E and G, ventral view; the others, right lateral view.
Pristionchus trametes n. sp. adults. A: Surface of the lip region. B, C: Stomatal region. D: Anterior part of an adult. E: Vulval region. F, G: Female tail (ph, phasmid). H: Male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; ph, phasmid). I: Male tail tip (labels are same as H). J: Spicule and gubernaculum. A and B, left lateral view; E and G, ventral view; the others, right lateral view.

Figure 3:

Pristionchus trametes n. sp. adults.A: Right lateral view of the stoma-pharyngeal region in four different focal planes (ep, secretory-excretory pore; car, cardia; nr, nerve ring; dr, deirid). B: Left lateral view of the stomatal region in three different focal planes (rsv, right subventral tooth; d, dorsal tooth; lsv, left subventral denticles).
Pristionchus trametes n. sp. adults.A: Right lateral view of the stoma-pharyngeal region in four different focal planes (ep, secretory-excretory pore; car, cardia; nr, nerve ring; dr, deirid). B: Left lateral view of the stomatal region in three different focal planes (rsv, right subventral tooth; d, dorsal tooth; lsv, left subventral denticles).

Figure 4:

Pristionchus trametes n. sp. adults. A: Left lateral view of the entire male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; co, cloacal opening). B: Spicule and gubernaculum in left lateral view. C: Left lateral view of the distal part of the male tail in two different focal planes (v + number, pd, genital papillae; ph, phasmid). D: Right lateral view of the female tail in two different focal planes (an, anus; ph, phasmid; rec, rectum). E: Ventral view of the female tail in four different focal planes (an, anus; ph, phasmid; rg, rectal glands).
Pristionchus trametes n. sp. adults. A: Left lateral view of the entire male tail (v + number, ad, genital papillae where “d” indicates laterally oriented papillae; co, cloacal opening). B: Spicule and gubernaculum in left lateral view. C: Left lateral view of the distal part of the male tail in two different focal planes (v + number, pd, genital papillae; ph, phasmid). D: Right lateral view of the female tail in two different focal planes (an, anus; ph, phasmid; rec, rectum). E: Ventral view of the female tail in four different focal planes (an, anus; ph, phasmid; rg, rectal glands).

Figure 5:

Phylogenetic relationships of Pristionchus spp. A Bayesian tree was inferred from concatenated SSU and D1-D4 LSU sequences under the GTR + G + I model. The following analytical parameters were used: SSU, freqA = 0.25, freqC = 0.21, freqG = 0.26, freqT = 0.28, R(a) = 0.77, R(b) = 2.52, R(c) = 2.09, R(d) = 0.83, R(e) = 3.32, R(f) = 1.00, Pinva = 0.46, Shape = 0.48; D1-D4 LSU, freqA = 0.23, freqC = 0.22, freqG = 0.31, freqT = 0.25, R(a) = 0.57, R(b) = 2.43, R(c) = 0.94, R(d) = 0.68, R(e) = 4.39, R(f) = 1.00, Pinva = 0.39, Shape = 0.49. Posterior probability values exceeding 50% are shown for each clade.
Phylogenetic relationships of Pristionchus spp. A Bayesian tree was inferred from concatenated SSU and D1-D4 LSU sequences under the GTR + G + I model. The following analytical parameters were used: SSU, freqA = 0.25, freqC = 0.21, freqG = 0.26, freqT = 0.28, R(a) = 0.77, R(b) = 2.52, R(c) = 2.09, R(d) = 0.83, R(e) = 3.32, R(f) = 1.00, Pinva = 0.46, Shape = 0.48; D1-D4 LSU, freqA = 0.23, freqC = 0.22, freqG = 0.31, freqT = 0.25, R(a) = 0.57, R(b) = 2.43, R(c) = 0.94, R(d) = 0.68, R(e) = 4.39, R(f) = 1.00, Pinva = 0.39, Shape = 0.49. Posterior probability values exceeding 50% are shown for each clade.

GenBank accession numbers for the sequences used for the phylogenetic analysis_

SpeciesSSULSU
Parapristionchus giblindavisi JX163981JX163972
Pristionchus aerivorus FJ040440KT188862
Pristionchus americanus FJ040445KT188867
Pristionchus arcanus KT188848KT188878
Pristionchus atlanticus KT188839KT188869
Pristionchus boliviae KT188838KT188868
Pristionchus borbonicus KT188856KT188885
Pristionchus brevicauda KT188841KT188871
Pristionchus bucculentus KT188860KT188889
Pristionchus bulgaricus KT188845KT188875
Pristionchus clavus KT188842KT188872
Pristionchus degawai MH114984
Pristionchus elegans KJ877238KJ877274
Pristionchus entomophagus FJ040441KT188873
Pristionchus exspectatus KT188849KT188879
Pristionchus fissidentatus KT188855KJ877273
Pristionchus fukushimae KT188852KT188882
Pristionchus hongkongensis MH114985
Pristionchus hoplostomus KT188853KT188883
Pristionchus japonicus KT188850KT188880
Pristionchus laevicollis MH114986
Pristionchus lheritieri SB245KT188846KT188876
Pristionchus lucani KT188844KT188874
Pristionchus marianneae FJ040442KT188866
Pristionchus maupasi FJ040443KT188863
Pristionchus maxplancki KT188851KT188881
Pristionchus mayeri KT188835KT188865
Pristionchus neolucani MH114987
Pristionchus occultus KX113518
Pristionchus pacificus PS312AF083010EU195982
Pristionchus pauli FJ040446KT188870
Pristionchus paulseni MH114982
Pristionchus pseudaerivus FJ040447KT188864
Pristionchus quartusdecimus KT188847KT188877
Pristionchus racemosae KT188859KT188888
Pristionchus riukiariae MH114988
Pristionchus sycomori KT188857KT188886
Pristionchus triformis KT188854KT188884
Pristionchus uniformis KJ877236KJ877272
Pristionchus yamagatae MH114983
Pristionchus sp. 2 CW-2016KX113519
Pristionchus sp. 3 CW-2016KX113517
Pristionchus sp. 35 VS-2015KT188858KT188887
Pristionchus sp. 38 VS-2015KT188861KT188890
Pristionchus trametes n. sp.LC633357

Morphometric values for Pristioinchus trametes n_ sp_

MaleFemale
HolotypeParatypesParatypes
N 910
L 712696 ± 58 (615–785)819 ± 41 (746–893)
a 24.123.9 ± 1.7 (20.9–26.1)19.9 ± 0.7 (18.4–20.9)
b 6.36.5 ± 0.5 (5.9–7.6)7.2 ± 0.4 (6.7–7.6)
c 12.411.9 ± 0.6 (11.1–13.3)12.3 ± 1.2 (10.2–13.9)
c′ 2.42.4 ± 0.1 (2.2–2.6)3.6 ± 0.5 (2.8–4.4)
T or V78.084.5 ± 3.3 (77.6–88.9)51.6 ± 0.8 (50.4–52.8)
Anterior pharynx5955 ± 2.8 (49–59)57 ± 3.0 (54–64)
Posterior pharynx4948 ± 2.2 (43–52)51 ± 3.1 (45–55)
Anterior/posterior pharynx ratio1.11.1 ± 0.1 (1.0–1.2)1.1 ± 0.1 (1.0–1.2)
Nerve ring from anterior end7975 ± 3.9 (68–81)77 ± 4.0 (72–85)
Secretory-excretory pore from anterior end10299 ± 5.0 (93–110)104 ± 5.0 (96–112)
Deirid from anterior end117116 ± 4.9 (110–126)127 ± 5.0 (117–134)
Median bulb diam.17.616.9 ± 0.7 (15.8–18.0)19.0 ± 0.7 (18.0–19.8)
Basal bulb diam.14.312.9 ± 1.0 (11.0–14.4)14.3 ± 0.7 (13.3–15.1)
Maximum body diam.3029 ± 3.2 (25–36)41 ± 2.4 (37–43)
Cloacal or anal body diam.2424 ± 1.6 (22–27)19 ± 1.1 (14–22)
Tail length5859 ± 3.9 (52–65)67 ± 7.1 (60–79)
Gonad length553588 ± 50 (519–668)
Vas deferens length102110 ± 14 (95–136)
Ratio of vas deferens to total gonad (%)1819 ± 1.3 (17–21)
Spicule (arc)4039 ± 2.1 (36–42)
Spicule (chord)3331 ± 1.7 (29–33)
Gubernaculum (chord)16.817.6 ± 1.4 (15.8–19.9)
Tail spike length13.812.9 ± 2.1 (9.2–16.8)
Ratio (%) of tail spike to total tail2422 ± 2.9 (16–26)
Anterior ovary255 ± 17 (229–278)
Posterior ovary286 ± 54 (214–363)
Vulval body diam.41 ± 2.1 (37–43)
Anus-phasmid38 ± 4.1 (34–48)
Relative position of phasmid to total tail (%)57 ± 5.6 (48–66)
DOI: https://doi.org/10.21307/jofnem-2021-060 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 13
Published on: Jul 19, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2021 Natsumi Kanzaki, Keiko Hamaguchi, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.