
Figure 1:
Effects of orange peel power (OPP) amendment on the mortality of J2 of M. incognita in vitro by B. velezensis AP203.
Table 1.
Effect of B. velezensis AP203 amended with orange peel powder (OPP) or glucose on soybean growth at 45 days after planting in greenhouse trials.
| Treatmenta | Shoot length (cm)b | Root length (cm) | Shoot fresh weight (g) | Root fresh weight (g) | ||||
|---|---|---|---|---|---|---|---|---|
| TSB-grown cells | 58.0 | a | 22.5 | ab | 6.51 | ab | 3.91 | ab |
| OPP (supernatant) | 67.4 | a | 21.9 | abc | 8.12 | ab | 4.20 | ab |
| Glucose (supernatant) | 66.8 | a | 19.9 | cde | 7.55 | ab | 3.98 | ab |
| OPP (Cells) | 72.3 | a | 22.0 | abc | 9.06 | ab | 4.92 | a |
| Glucose (Cells) | 62.5 | a | 20.1 | bcde | 8.43 | ab | 3.42 | ab |
| OPP (Culture) | 72.0 | a | 22.6 | a | 10.20 | a | 4.11 | ab |
| Glucose (Culture) | 60.8 | a | 19.0 | e | 8.25 | ab | 4.00 | ab |
| OPP | 62.5 | a | 21.4 | abcd | 8.12 | ab | 3.65 | ab |
| Glucose | 67.9 | a | 20.0 | cde | 5.95 | ab | 2.53 | b |
| M. incognita nematode | ||||||||
| Untreated control | ||||||||
Table 2.
Effect of B. velezensis AP203, amended orange peel powder (OPP) or glucose on cotton growth at 45 days after planting in greenhouse trials.
| Shoot length | Root length | Shoot fresh | Root fresh | |||||
|---|---|---|---|---|---|---|---|---|
| Treatmenta | (cm)b | (cm) | weight (g) | weight (g) | ||||
| TSB-grown cells | 26.0 | ab | 18.5 | abc | 2.6 | a | 1.7 | a |
| OPP (supernatant) | 27.3 | ab | 20.2 | ab | 2.5 | a | 1.7 | a |
| Glucose (supernatant) | 26.8 | ab | 18.2 | abc | 2.5 | a | 1.6 | a |
| OPP (Cells) | 30.7 | a | 21.2 | ab | 2.2 | a | 1.9 | a |
| Glucose (Cells) | 27.0 | ab | 16.1 | bc | 2.7 | a | 1.6 | a |
| OPP (Culture) | 30.6 | ab | 20.1 | ab | 2.4 | a | 1.4 | a |
| Glucose (Culture) | 28.8 | ab | 18.1 | abc | 2.5 | a | 1.6 | a |
| OPP | 23.2 | ab | 17.7 | abc | 2.1 | a | 1.7 | a |
| Glucose | 22.6 | b | 16.1 | bc | 2.3 | a | 1.1 | a |
| M. incognita nematode | 20.0 | b | 14.3 | c | 1.7 | a | 0.9 | a |
| Untreated control | 26.2 | ab | 21.8 | a | 2.7 | a | 1.3 | a |

Figure 2:
Effects of B. velezensis AP203 with an orange peel power (OPP) or glucose amendments on the number of M. incognita eggs on the roots of soybean at 45 days after planting in greenhouse trials.

Figure 3:
Effects of B. velezensis AP203 with an orange peel power (OPP) or glucose amendments on the number of M. incognita eggs on the roots of cotton at 45 days after planting in greenhouse trials.
Table 3.
Predicted secondary metabolites found in cell-free supernatants of B. velezensis AP203 after 48 hr growth in 0.5% (w/v) OPP amended Tris Spizizen Salts (TSS) media.
| Treatmenta | RT (min)b | Product ions (m/z) | RA/CFUc | Predicted metabolites |
|---|---|---|---|---|
| OPAP203 1 | 6.69 min | 211.11 | 21.11 | 1,3-Diphenyl-2-propanone |
| OPAP203 2 | 6.63 min | 253.12 | 25.31 | p-(3,4-Dihydro-6-methoxy-2-naphthyl) phenol |
| OPAP203 3 | 3.39 min | 295.17 | 29.51 | (E)-1,1,-(1,2-Diethyl-1,2-ethenediyl) bis (4-methoxybenzene) |
| OPAP203 4 | 2.40 min | 206.12 | 20.61 | 3-(Dimethylamino) propyl benzoate |
| GluAP203 | 0 | 0 | 0 | 0 |
| OPP | 0 | 0 | 0 | 0 |
| Glucose | 0 | 0 | 0 | 0 |
Table S1
List of predicted secondary metabolites found in cell-free supernatants of B. velezensis AP203 after 48 hr growth in 0.5% (w/v) orange peel power (OPP) amended Tris Spizizen Salts (TSS) media.
| Query ID | Query m/z | Name of the bioactive compounds | Formula | Exact mass |
|---|---|---|---|---|
| 1 | 101.0711 | Gyromitrin;Acetaldehyde methylformylhydrazone | C4H8N2O | 100.0636629 |
| 2 | 103.0559 | Indoleacetic acid | C10H9NO2 | 175.0633285 |
| 3 | 103.0559 | 5-Hydroxyindoleacetaldehyde | C10H9NO2 | 175.0633285 |
| 4 | 104.0549 | 2-Ethyl-1-hexanol, 9CI; (±)-form, O-Sulfate | C8H18O4S | 210.0925798 |
| 5 | 104.0568 | 2-Ethyl-1-hexanol, 9CI; (±)-form, O-Sulfate | C8H18O4S | 210.0925798 |
| 7 | 104.0585 | Histidinyl-Glycine | C8H12N4O3 | 212.0909403 |
| 8 | 104.0585 | D-Glycero-D-galacto-heptitol | C7H16O7 | 212.0896029 |
| 9 | 104.0585 | Glycyl-Histidine | C8H12N4O3 | 212.0909403 |
| 10 | 104.0707 | N-methyl-beta-alanine | C4H9NO2 | 103.0633285 |
| 11 | 104.0707 | (2S)-2-Nitrobutane | C4H9NO2 | 103.0633285 |
| 13 | 104.0707 | Ethyl carbamic acid methyl ester | C4H9NO2 | 103.0633285 |
| 14 | 104.0707 | N-Methyl-L-alanine | C4H9NO2 | 103.0633285 |
| 15 | 104.0707 | HBA | C4H9NO2 | 103.0633285 |
| 16 | 104.0707 | DL-3-aminobutyrate | C4H9NO2 | 103.0633285 |
| 17 | 104.0707 | Mefenamic acid | C15H15NO2 | 241.1102787 |
| 18 | 104.0707 | N-[2-(4-Hydroxyphenyl)ethyl]benzamide | C15H15NO2 | 241.1102787 |
| 19 | 104.0707 | 2-Amino-2-methylpropanoate;2-Aminoisobutyric acid | C4H9NO2 | 103.0633285 |
| 20 | 104.0707 | (R,S)-3-Amino-2-methylpropanoate | C4H9NO2 | 103.0633285 |
| 21 | 104.0707 | beta-alanine-methyl-ester | C4H9NO2 | 103.0633285 |
| 22 | 104.0707 | N,N-Dimethylglycine;Dimethylglycine | C4H9NO2 | 103.0633285 |
| 23 | 104.0707 | N-Ethylglycine | C4H9NO2 | 103.0633285 |
| 24 | 104.0707 | (R)-2-Aminobutanoic acid;(S)-2-Aminobutanoate | C4H9NO2 | 103.0633285 |
| 25 | 104.0707 | 1-nitrobutane | C4H9NO2 | 103.0633285 |
| 26 | 104.0707 | 4-Aminobutanoate;4-Aminobutanoic acid | C4H9NO2 | 103.0633285 |
| 27 | 105.0366 | 3-methylthiopropanal | C4H8OS | 104.0295856 |
| 28 | 105.0366 | Acutifolane A | C16H22O3 | 262.1569 |
| 29 | 105.0366 | tetrahydrothiophene 1-oxide | C4H8OS | 104.0295856 |
| 30 | 105.0367 | 3-methylthiopropanal | C4H8OS | 104.0295856 |
| 31 | 105.0367 | tetrahydrothiophene 1-oxide | C4H8OS | 104.0295856 |
| 32 | 105.0372 | 3-methylthiopropanal | C4H8OS | 104.0295856 |
| 33 | 105.0372 | tetrahydrothiophene 1-oxide | C4H8OS | 104.0295856 |
| 34 | 105.0376 | 3-methylthiopropanal | C4H8OS | 104.0295856 |
| 35 | 105.0376 | tetrahydrothiophene 1-oxide | C4H8OS | 104.0295856 |
| 37 | 105.044 | 3-cyanopyridine | C6H4N2 | 104.0374481 |
| 38 | 105.044 | 2-Cyanopyridine | C6H4N2 | 104.0374481 |
| 39 | 105.044 | 4-Cyanopyridine | C6H4N2 | 104.0374481 |
| 40 | 105.0441 | (+)-18-Hydroxy-7,16-sacculatadiene-11,12-dial | C20H30O3 | 318.2195 |
| 41 | 105.0441 | ent-7alpha-hydroxykaur-16-en-19-oic acid | C20H30O3 | 318.2195 |
| 42 | 105.0441 | 2-Cyanopyridine | C6H4N2 | 104.0374481 |
| 43 | 105.0441 | Oxymesterone | C20H30O3 | 318.2194948 |
| 44 | 105.0441 | 4-Cyanopyridine | C6H4N2 | 104.0374481 |
| 45 | 105.0441 | 3-cyanopyridine | C6H4N2 | 104.0374481 |
| 46 | 105.0441 | 8-oxo-5E,9Z,11Z,14Z-eicosatetraenoic acid | C20H30O3 | 318.2195 |
| 47 | 105.0441 | 9-oxo-5E,7Z,11Z,14Z-eicosatetraenoic acid | C20H30O3 | 318.2195 |
| 48 | 105.0441 | 11-oxo-5E,8Z,12Z,14Z-Eicosatetraenoic acid | C20H30O3 | 318.2195 |
| 49 | 105.0441 | (+)-7beta-Hydroxy-15-beyeren-19-oic acid | C20H30O3 | 318.2195 |
| 50 | 105.0557 | Tyrosyl-Tyrosine | C18H20N2O5 | 344.1372218 |
| 51 | 105.0651 | Aminoserine | C3H8N2O2 | 104.0585775 |
| 52 | 105.0651 | L-2,3-Diaminopropionate | C3H8N2O2 | 104.0585775 |
| 53 | 105.0651 | Hydroxyaminoalanine | C3H8N2O2 | 104.0585775 |
| 54 | 105.0662 | 2,3-Diaminopropanoic acid | C3H8N2O2 | 104.0585775 |
| 55 | 105.0672 | 4,-O-Methylbavachalcone | C22H24O4 | 352.1675 |
| 56 | 105.0672 | Ovalichalcone | C22H24O4 | 352.1675 |
| 57 | 105.0672 | Pongagallone A | C22H24O4 | 352.1675 |
| 58 | 105.0672 | Candidone | C22H24O4 | 352.1675 |
| 59 | 105.0672 | Methylhildgardtol A | C22H24O4 | 352.1675 |
| 60 | 105.0672 | Methylhildgardtol B | C22H24O4 | 352.1675 |
| 61 | 105.0672 | Xuulanin | C22H24O4 | 352.1675 |
| 62 | 105.0715 | Valganciclovir | C14H22N6O5 | 354.1651678 |
| 63 | 105.0733 | 12-hydroxyjasmonic acid 12-O-beta-D-glucoside | C19H30O8 | 386.1941 |
| 64 | 105.0733 | Citroside A | C19H30O8 | 386.1940679 |
| 65 | 105.0733 | 6,9-Dihydroxy-4,7-megastigmadien-3-one | C19H30O8 | 386.1940679 |
| 66 | 107.0845 | p-Xylene;1,4-Dimethylbenzene;p-Methyltoluene | C8H10 | 106.0782503 |
| 67 | 107.0845 | Ethylbenzene;Phenylethane;Ethylbenzol;Ethylenzene | C8H10 | 106.0782503 |
| 68 | 107.0845 | o-Xylene;o-Dimethylbenzene;o-Methyltoluene | C8H10 | 106.0782503 |
| 69 | 107.0845 | m-Xylene;1,3-Dimethylbenzene;1,3-Xylene | C8H10 | 106.0782503 |
| 70 | 107.0848 | o-Xylene;o-Dimethylbenzene;o-Methyltoluene | C8H10 | 106.0782503 |
| 71 | 107.0848 | m-Xylene;1,3-Dimethylbenzene;1,3-Xylene | C8H10 | 106.0782503 |
| 72 | 107.0848 | p-Xylene;1,4-Dimethylbenzene;p-Methyltoluene | C8H10 | 106.0782503 |
| 73 | 107.0848 | Ethylbenzene;Phenylethane;Ethylbenzol;Ethylenzene | C8H10 | 106.0782503 |
| 74 | 107.0848 | O-6-deoxy-a-L-galactopyranosyl | C20H33NO14 | 511.1901048 |
| 75 | 107.0851 | o-Xylene;o-Dimethylbenzene;o-Methyltoluene | C8H10 | 106.0782503 |
| 76 | 107.0851 | m-Xylene;1,3-Dimethylbenzene;1,3-Xylene | C8H10 | 106.0782503 |
| 77 | 107.0851 | p-Xylene;1,4-Dimethylbenzene;p-Methyltoluene | C8H10 | 106.0782503 |
| 78 | 107.0851 | Ethylbenzene;Phenylethane;Ethylbenzol;Ethylenzene | C8H10 | 106.0782503 |
| 79 | 107.0858 | p-Xylene;1,4-Dimethylbenzene;p-Methyltoluene | C8H10 | 106.0782503 |
| 80 | 107.0858 | Ethylbenzene;Phenylethane;Ethylbenzol;Ethylenzene | C8H10 | 106.0782503 |
| 81 | 107.0858 | o-Xylene;o-Dimethylbenzene;o-Methyltoluene | C8H10 | 106.0782503 |
| 82 | 107.0858 | m-Xylene;1,3-Dimethylbenzene;1,3-Xylene | C8H10 | 106.0782503 |
| 83 | 107.0858 | Daunorubicin | C27H29NO10 | 527.1791462 |
| 84 | 107.0859 | m-Xylene;1,3-Dimethylbenzene;1,3-Xylene | C8H10 | 106.0782503 |
| 85 | 107.0859 | p-Xylene;1,4-Dimethylbenzene;p-Methyltoluene | C8H10 | 106.0782503 |
| 86 | 107.0859 | Ethylbenzene;Phenylethane;Ethylbenzol;Ethylenzene | C8H10 | 106.0782503 |
| 87 | 107.0859 | o-Xylene;o-Dimethylbenzene;o-Methyltoluene | C8H10 | 106.0782503 |
| 88 | 109.0287 | 1,2-Benzoquinone | C6H4O2 | 108.0211294 |
| 89 | 109.0287 | Quinone;p-Benzoquinone;Chinone | C6H4O2 | 108.0211294 |
| 90 | 109.0306 | Hordatine B glucoside | C35H50N8O10 | 742.3649899 |
| 91 | 109.0306 | Hydroxymethylmethylsilanediol | C2H8O3Si | 108.0242707 |
| 92 | 109.0309 | Hydroxymethylmethylsilanediol | C2H8O3Si | 108.0242707 |
| 93 | 109.0309 | Monothioglycerol | C3H8O2S | 108.0245002 |
| 94 | 109.0315 | Hydroxymethylmethylsilanediol | C2H8O3Si | 108.0242707 |
| 95 | 109.0316 | Hydroxymethylmethylsilanediol | C2H8O3Si | 108.0242707 |
| 96 | 109.0316 | Hydroxymethylmethylsilanediol | C2H8O3Si | 108.0242707 |
| 97 | 109.0321 | Hydroxymethylmethylsilanediol | C2H8O3Si | 108.0242707 |
| 98 | 109.0642 | Benzenemethanol;Phenylmethanol;Phenylcarbinol | C7H8O | 108.0575149 |
| 99 | 109.0642 | o-Cresol;2-Hydroxytoluene;o-Methylphenol | C7H8O | 108.0575149 |
| 100 | 109.0642 | 3-Cresol;m-Cresol;3-Hydroxytoluene | C7H8O | 108.0575149 |
| 101 | 109.0642 | 4-Cresol;p-Cresol;4-Hydroxytoluene | C7H8O | 108.0575149 |
| 102 | 109.0642 | Anisole;Methoxybenzene;Methyl phenyl ether | C7H8O | 108.0575149 |
| 103 | 111.0434 | Resorcinol;Resorcin;1,3-Benzenediol | C6H6O2 | 110.0367794 |
| 104 | 111.0434 | Hydroquinone;p-Benzenediol;1,4-Benzenediol | C6H6O2 | 110.0367794 |
| 105 | 111.0434 | 5-Methyl-2-furaldehyde;5-Methyl-2-furfural | C6H6O2 | 110.0367794 |
| 106 | 111.0434 | o-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 107 | 111.0434 | Catechol;1,2-Benzenediol;o-Benzenediol | C6H6O2 | 110.0367794 |
| 108 | 111.0434 | Benzosemiquinone;p-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 109 | 111.0437 | Resorcinol;Resorcin;1,3-Benzenediol | C6H6O2 | 110.0367794 |
| 110 | 111.0437 | Hydroquinone;p-Benzenediol;1,4-Benzenediol | C6H6O2 | 110.0367794 |
| 111 | 111.0437 | 5-Methyl-2-furaldehyde;5-Methyl-2-furfural | C6H6O2 | 110.0367794 |
| 112 | 111.0437 | Catechol;1,2-Benzenediol;o-Benzenedio | C6H6O2 | 110.0367794 |
| 113 | 111.0437 | Benzosemiquinone;p-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 114 | 111.0438 | 2-Furanmethanol | C5H6O2 | 98.03677944 |
| 115 | 111.0438 | Benzosemiquinone;p-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 116 | 111.0438 | Resorcinol;Resorcin;1,3-Benzenediol | C6H6O2 | 110.0367794 |
| 117 | 111.0438 | Hydroquinone;p-Benzenediol;1,4-Benzenediol | C6H6O2 | 110.0367794 |
| 118 | 111.0438 | 5-Methyl-2-furaldehyde;5-Methyl-2-furfural | C6H6O2 | 110.0367794 |
| 119 | 111.0438 | o-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 120 | 111.0438 | penta-2,4-dienoic acid;beta-vinyl acrylic acid | C5H6O2 | 98.0368 |
| 121 | 111.0438 | Catechol;1,2-Benzenediol;o-Benzenediol | C6H6O2 | 110.0367794 |
| 122 | 111.0438 | 5-Methyl-2(3H)-furanone | C5H6O2 | 98.03677944 |
| 123 | 111.0447 | o-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 124 | 111.0447 | Catechol;1,2-Benzenediol;o-Benzenediol | C6H6O2 | 110.0367794 |
| 125 | 111.0447 | Benzosemiquinone;p-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 126 | 111.0447 | Resorcinol;Resorcin;1,3-Benzenediol;1,3-Dihydroxybenzene | C6H6O2 | 110.0367794 |
| 127 | 111.0447 | Hydroquinone;p-Benzenediol;1,4-Benzenediol | C6H6O2 | 110.0367794 |
| 128 | 111.0447 | 5-Methyl-2-furaldehyde;5-Methyl-2-furfural | C6H6O2 | 110.0367794 |
| 129 | 111.0448 | o-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 130 | 111.0448 | Catechol;1,2-Benzenediol;o-Benzenediol | C6H6O2 | 110.0367794 |
| 131 | 111.0448 | Benzosemiquinone;p-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 132 | 111.0448 | Resorcinol;Resorcin;1,3-Benzenediol;1,3-Dihydroxybenzene | C6H6O2 | 110.0367794 |
| 133 | 111.0448 | Hydroquinone;p-Benzenediol;1,4-Benzenediol | C6H6O2 | 110.0367794 |
| 134 | 111.0451 | Catechol;1,2-Benzenediol;o-Benzenediol | C6H6O2 | 110.0367794 |
| 135 | 111.0451 | Benzosemiquinone;p-Benzosemiquinone | C6H6O2 | 110.0367794 |
| 136 | 111.0451 | Resorcinol;Resorcin;1,3-Benzenediol;1,3-Dihydroxybenzene | C6H6O2 | 110.0367794 |
| 137 | 111.0451 | Hydroquinone;p-Benzenediol;1,4-Benzenediol | C6H6O2 | 110.0367794 |
| 138 | 111.0451 | 5-Methyl-2-furaldehyde;5-Methyl-2-furfural | C6H6O2 | 110.0367794 |
| 139 | 121.0316 | Dimethylsulfonioacetate | C4H8O2S | 120.0245002 |
| 140 | 121.0316 | 3-(Methylthio)propionic acid;3-Methylthiopropionate | C4H8O2S | 120.0245002 |
| 141 | 121.0316 | sulfolane | C4H8O2S | 120.0245002 |
| 142 | 121.032 | Dimethylsulfonioacetate | C4H8O2S | 120.0245002 |
| 143 | 121.032 | 3-(Methylthio)propionic acid;3-Methylthiopropionate | C4H8O2S | 120.0245002 |
| 144 | 121.032 | sulfolane | C4H8O2S | 120.0245002 |
| 145 | 121.0324 | Dimethylsulfonioacetate | C4H8O2S | 120.0245002 |
| 146 | 121.0324 | 3-(Methylthio)propionic acid;3-Methylthiopropionate | C4H8O2S | 120.0245002 |
| 147 | 121.0324 | sulfolane | C4H8O2S | 120.0245002 |
| 148 | 121.0325 | Dimethylsulfonioacetate | C4H8O2S | 120.0245002 |
| 149 | 121.0325 | 3-(Methylthio)propionic acid;3-Methylthiopropionate | C4H8O2S | 120.0245002 |
| 150 | 121.0325 | sulfolane | C4H8O2S | 120.0245002 |
| 151 | 121.0325 | Dimethylsulfonioacetate | C4H8O2S | 120.0245002 |
| 152 | 121.0325 | 3-(Methylthio)propionic acid;3-Methylthiopropionate | C4H8O2S | 120.0245002 |
| 153 | 121.0325 | sulfolane | C4H8O2S | 120.0245002 |
| 154 | 121.0325 | Dimethylsulfonioacetate | C4H8O2S | 120.0245002 |
| 155 | 121.0325 | 3-(Methylthio)propionic acid;3-Methylthiopropionate | C4H8O2S | 120.0245002 |
| 156 | 121.0325 | sulfolane | C4H8O2S | 120.0245002 |
| 157 | 121.037 | 3-nitro-1-propionate | C3H6NO4 | 120.0296827 |
| 158 | 121.0379 | 3-nitro-1-propionate | C3H6NO4 | 120.0296827 |
| 159 | 121.05 | 2,3-dihydroxy-2-methyl-propanoic acid | C4H8O4 | 120.0422587 |
| 160 | 121.05 | L-(+)-Erythrose;D-threo-Aldose;D-Erythrulose | C4H8O4 | 120.0422587 |
| 161 | 121.05 | 3-Deoxytetronic acid | C4H8O4 | 120.0422587 |
| 162 | 121.05 | 4-Deoxyerythronic acid | C4H8O4 | 120.0422587 |
| 163 | 121.05 | L-Erythrulose;L-glycero-Tetrulose | C4H8O4 | 120.0422587 |
| 164 | 121.05 | 3,4-Dihydroxybutyric acid | C4H8O4 | 120.0422587 |
| 165 | 121.05 | D-Threose;D-threo-Tetrose;D-Erythrose | C4H8O4 | 120.0422587 |
| 166 | 121.0516 | Purine | C5H4N4 | 120.0435961 |
| 167 | 122.0962 | 3,4-DIMETHYLANILINE | C8H11N | 121.0891494 |
| 168 | 122.0962 | 1-Phenylethylamine;alpha-Phenylethylamine | C8H11N | 121.0891494 |
| 169 | 122.0962 | N-Ethylaniline;N-Ethylbenzenamine | C8H11N | 121.0891494 |
| 170 | 122.0962 | Phenethylamine;2-Phenylethylamine;beta-Phenylethylamine | C8H11N | 121.0891494 |
| 171 | 122.0962 | 2,4-Dimethylaniline;2,4-DMA | C8H11N | 121.0891494 |
| 172 | 122.0962 | N,N-Dimethylaniline; N,N-Dimethylbenzenamine | C8H11N | 121.0891494 |
| 173 | 122.0965 | 1-Phenylethylamine;alpha-Phenylethylamine | C8H11N | 121.0891494 |
| 174 | 122.0965 | 2,6-Dimethylaniline | C8H11N | 121.0891494 |
| 175 | 122.0965 | N-Ethylaniline;N-Ethylbenzenamine | C8H11N | 121.0891494 |
| 176 | 122.0965 | 2,5-Dimethylalanine | C8H11N | 121.0891494 |
| 177 | 122.0965 | 2-Phenylethylamine;beta-Phenylethylamine | C8H11N | 121.0891494 |
| 178 | 181.0694 | Sorbose;xylo-Hexulose;D-Fructose | C6H12O6 | 180.0633881 |
| 179 | 181.0694 | 2-Deoxy-D-gluconate | C6H12O6 | 180.0633881 |
| 180 | 181.0694 | Ketose | C6H12O6 | 180.0633881 |
| 181 | 181.0996 | Methylphophonic acid diisopropyl ester | C7H17O3P | 180.0915309 |
| 182 | 229.1235 | 1,1-Bis(4-hydroxyphenyl)propane | C15H16O2 | 228.1150298 |
| 183 | 229.1235 | Mansonone C | C15H16O2 | 228.1150298 |
| 184 | 229.1235 | Bisphenol A;2,2-Bis(4-Hydroxyphenyl)propane | C15H16O2 | 228.1150298 |
| 185 | 229.1235 | dihydropinosylvin monomethylether | C15H16O2 | 228.1150298 |
| 186 | 229.1285 | Deoxyguanidinoproclavaminic acid | C9H16N4O3 | 228.1222404 |
| 187 | 229.1298 | Deoxyamidinoproclavaminate | C9H16N4O3 | 228.1222404 |
| 188 | 230.058 | Lamivudine;3TC;2’,3’-Dideoxy-3’-thiacytidine | C8H11N3O3S | 229.0521119 |
| 189 | 230.058 | Carbonylphophonic acid | C9H12NO4P | 229.0503944 |
| 190 | 230.058 | 2,3-Dihydroxy-2’-carboxybiphenyl | C13H9O4- | 229.0500838 |
| 191 | 357.1718 | Rutamarin | C21H24O5 | 356.1623739 |
| 191 | 357.1718 | Gingerenone A | C21H24O5 | 356.1623739 |
| 192 | 357.1718 | alpha,beta-dihydroxanthohumol | C21H24O5 | 356.1623739 |
| 193 | 357.1718 | Kadsurenone;Denudatin B | C21H24O5 | 356.1623739 |
| 194 | 371.0795 | Rebamipide | C19H15ClN2O4 | 370.0720347 |
| 195 | 371.0795 | Digalacturonate;Digalacturonic acid | C12H18O13 | 370.0747407 |
| 196 | 371.0795 | 1,2-beta-D-Glucuronosyl-D-glucuronate | C12H18O13 | 370.0747407 |
| 197 | 371.145 | Napththalene-2-sulfonamide | C20H22N2O3S | 370.1351133 |
| 198 | 371.1501 | iso-dehydrocycloxanthohumol hydrate | C21H22O6 | 370.1416384 |
| 199 | 371.1501 | xanthohumol D | C21H22O6 | 370.1416384 |
| 200 | 371.1501 | 5’-Prenylhomoeriodictyol;Sigmoidin B 3’-methyl ether | C21H22O6 | 370.1416384 |
| 201 | 371.1501 | xanthohumol B | C21H22O6 | 370.1416384 |
| 202 | 371.1501 | curcumin | C21H22O6 | 370.1416384 |
| 203 | 371.1501 | Alkannin beta,beta-dimethylacrylate | C21H22O6 | 370.1416384 |
| 204 | 371.1501 | Sophoraisoflavanone A | C21H22O6 | 370.1416384 |
| 205 | 371.1509 | iso-dehydrocycloxanthohumol hydrate | C21H22O6 | 370.1416384 |
| 206 | 371.1509 | xanthohumol D | C21H22O6 | 370.1416384 |
| 207 | 371.1509 | 5’-Prenylhomoeriodictyol;Sigmoidin B 3’-methyl ether | C21H22O6 | 370.1416384 |
| 208 | 371.1509 | xanthohumol B | C21H22O6 | 370.1416384 |
| 209 | 371.1509 | curcumin | C21H22O6 | 370.1416384 |
| 210 | 371.1509 | Alkannin beta,beta-dimethylacrylate | C21H22O6 | 370.1416384 |
| 211 | 371.1509 | Sophoraisoflavanone A | C21H22O6 | 370.1416384 |
| 212 | 371.1528 | Galactan;Amylose | C14H26O11 | 370.1475117 |
| 213 | 371.1528 | Galactan;Amylose | C14H26O11 | 370.1475117 |
| 214 | 371.1541 | Galactan;Amylose | C14H26O11 | 370.1475117 |
| 215 | 404.1365 | trifluoperazine | C21H20F3N3S | 403.1330029 |
| 216 | 404.1484 | Ampicillin trihydrate | C16H25N3O7S | 403.1413209 |
| 217 | 405.1191 | Spectinomycin dihydrochloride | C14H26Cl2N2O7 | 404.1117066 |
| 218 | 405.1267 | Sulfinpyrazone;Sulfoxyphenylpyrazolidine | C23H20N2O3S | 404.1194632 |
| 219 | 405.1269 | Sulfinpyrazone;Sulfoxyphenylpyrazolidine | C23H20N2O3S | 404.1194632 |

Figure S1:
LC-MS/MS spectra of the peaks eluted at 6.69 min (m/z of 211.11).

Figure S2:
LC-MS/MS spectra of the peaks eluted at 2.40 min (m/z of 206.12).

Figure S3:
LC-MS/MS spectra of the peaks eluted at 6.63 min (m/z of 253.12) and 3.39 min (m/z of 295.17).