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Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece Cover

Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece

Journal of Nematology
Volume 53 (2021): Issue 1 (January 2021)
By: ,  ,  ,  ,  ,   and    
Open Access
|Jan 2021
Figures & tables

Figures & Tables

Figure 1:

Light micrographs of Longidorus pauli (Lamberti et al., 1999) from Greece (A-N). A-E: Female anterior regions. F: Detail of base of odontostyle and odontophore, G: Vulval region showing sperm cells in the uterus, H-K: Female tail regions, L: Detail of sperm cells in male genital tract, M: Male tail region, N: Detail of spicules. a = anus; af = amphidial fovea; as = adanal supplement; c = cloaca; gr = guiding ring; Odt = odontostyle; Odp = odontophore; sp = spicules; spc = sperm cells; V = vulva. (Scale bars: A-N = 20 µm).

Figure 2:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from D2 and D3 expansion domains of 28S rRNA sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 3:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) from Greece within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from ITS1 rRNA sequence alignment under the TIM3 + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 4:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus trees as inferred from cytochrome c oxidase subunit I (coxI) mtDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are in bold letters.

Morphometrics of Longidorus pauli (Lamberti et al_, 1999) from Greece_

Thessaloniki, GreeceIdleb, Syria (Lamberti et al., 1999)
Charactera FemalesMalesFemalesMales
n 852012
L (mm)7.6 ± 0.66 (6.62-8.51)7.19 ± 0.27 (6.66-8.02)7.6 ± 0.51 (6.5-8.6)7.7 ± 0.64 (6.8-8.7)
a 147.9 ± 15.3 (125.0-168.8)150.1 ± 8.9 (138.3-162.5)131.6 ± 8.5 (120.3-143.5)139.9 ± 7.9 (130.8-154.8)
b 16.0 ± 1.7 (13.9-19.0)16.5 ± 1.3 (14.8-17.8)16.9 ± 1.6 (14.5-19.7)16.1 ± 1.8 (12.8-19.5)
c 173.1 ± 29.0 (126.9-224.0)158.9 ± 12.3 (146.1-175.3)200.7 ± 17.9 (163.5-220.0)181.9 ± 11.7 (166.0-197.8)
c’ 1.1 ± 0.1 (1.0-1.3)1.2 ± 0.1 (1.0-1.3)0.9 ± 0.1 (0.8-1.0)0.97 ± 0.05 (0.9-1.0)
d b 2.1 ± 0.2 (1.9-2.4)2.3 ± 0.2 (2.0-2.6)––
d’ c 1.6 ± 0.1 (1.4-1.8)1.5 ± 0.1 (1.4-1.6)––
V/Spicules length52.1 ± 2.2 (49.4-56.0)57.6 ± 0.4 (54.0-60.0)51 ± 1.5 (49.0-54.0)64.6 ± 2.5 (61.0-69.0)
Odontostyle length117.4 ± 5.5 (112.0-126.0)113.6 ± 7.4 (103.0-120.0)109.4 ± 3.6 (102.0-118.3)109.0 ± 4.7 (101.5-117.7)
Odontophore length63.5 ± 2.2 (61.0-67.0)63.4 ± 3.3 (59.0.68.0)61.2 ± 2.5 (56.0-64.0)63.0 ± 1.8 (61.0-66.5)
Total stylet length180.9 ± 6.8 (173.0-192.0)177.0 ± 6.7 (167.0-184.0)––
Anterior end to guide ring31.6 ± 1.9 (28.0-33.5)33.2 ± 1.3 (32.0-35.0)30.6 ± 2.1 (27.2-35.8)30.4 ± 1.6 (27.7-32.9)
Tail length42.9 ± 2.1 (38.0-45.0)46.6 ± 1.3 (44.0-49.0)37.8 ± 3.2 (31.5-45.0)42.4 ± 3.3 (36.4-46.3)
Hyaline part of tail length16.4 ± 1.5 (14.0-18.5)14.3 ± 2.0 (12.5-17.0)13.1 ± 1.1 (10.5-15.4)11.5 ± 1.6 (10.0-14.9)
Body width at level of
 lip region14.9 ± 0.7 (13.5-16.0)14.8 ± 1.0 (13.5-16.0)15.2 ± 0.9 (14.0-17.0)14.2 ± 0.5 (13.9-15.0)
 guide ring23.1 ± 1.5 (21-24.5)22.6 ± 1.5 (20.0-23.5)23.5 ± 1.1 (21.0-25.0)21.8 ± 1.0 (19.6-23.0)
 anus38.8 ± 1.3 (37.0-41.5)36.6 ± 1.5 (32.0-39.0)41.4 ± 2.4 (36.6-44.6)42.6 ± 2.3 (37.6-45.7)
DOI: https://doi.org/10.21307/jofnem-2021-034 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 10
Published on: Jan 1, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Cytochrome oxidase c subunit 1,
D2-D3 of 28S rRNA,
Description,
ITS1 rRNA,
Longidorus ,
L. pauli ,
L. pisi ,
Needle nematodes,
Taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2021 Emmanuel A. Tzortzakakis, Ilenia Clavero-Camacho, Carolina Cantalapiedra-Navarrete, Parthenopi Ralli, Juan E. Palomares-Rius, Pablo Castillo, Antonio Archidona-Yuste, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 53 (2021): Issue 1 (January 2021)
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