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Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece

Journal of Nematology
Volume 53 (2021): Issue 1 (January 2021)
By:
Open Access
|Jan 2021

Figures & Tables

Figure 1:

Light micrographs of Longidorus pauli (Lamberti et al., 1999) from Greece (A-N). A-E: Female anterior regions. F: Detail of base of odontostyle and odontophore, G: Vulval region showing sperm cells in the uterus, H-K: Female tail regions, L: Detail of sperm cells in male genital tract, M: Male tail region, N: Detail of spicules. a = anus; af = amphidial fovea; as = adanal supplement; c = cloaca; gr = guiding ring; Odt = odontostyle; Odp = odontophore; sp = spicules; spc = sperm cells; V = vulva. (Scale bars: A-N = 20 µm).
Light micrographs of Longidorus pauli (Lamberti et al., 1999) from Greece (A-N). A-E: Female anterior regions. F: Detail of base of odontostyle and odontophore, G: Vulval region showing sperm cells in the uterus, H-K: Female tail regions, L: Detail of sperm cells in male genital tract, M: Male tail region, N: Detail of spicules. a = anus; af = amphidial fovea; as = adanal supplement; c = cloaca; gr = guiding ring; Odt = odontostyle; Odp = odontophore; sp = spicules; spc = sperm cells; V = vulva. (Scale bars: A-N = 20 µm).

Figure 2:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from D2 and D3 expansion domains of 28S rRNA sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from D2 and D3 expansion domains of 28S rRNA sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 3:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) from Greece within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from ITS1 rRNA sequence alignment under the TIM3 + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) from Greece within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from ITS1 rRNA sequence alignment under the TIM3 + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 4:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus trees as inferred from cytochrome c oxidase subunit I (coxI) mtDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are in bold letters.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus trees as inferred from cytochrome c oxidase subunit I (coxI) mtDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are in bold letters.

Morphometrics of Longidorus pauli (Lamberti et al_, 1999) from Greece_

Thessaloniki, GreeceIdleb, Syria (Lamberti et al., 1999)
Charactera FemalesMalesFemalesMales
n 852012
L (mm)7.6 ± 0.66 (6.62-8.51)7.19 ± 0.27 (6.66-8.02)7.6 ± 0.51 (6.5-8.6)7.7 ± 0.64 (6.8-8.7)
a 147.9 ± 15.3 (125.0-168.8)150.1 ± 8.9 (138.3-162.5)131.6 ± 8.5 (120.3-143.5)139.9 ± 7.9 (130.8-154.8)
b 16.0 ± 1.7 (13.9-19.0)16.5 ± 1.3 (14.8-17.8)16.9 ± 1.6 (14.5-19.7)16.1 ± 1.8 (12.8-19.5)
c 173.1 ± 29.0 (126.9-224.0)158.9 ± 12.3 (146.1-175.3)200.7 ± 17.9 (163.5-220.0)181.9 ± 11.7 (166.0-197.8)
c’ 1.1 ± 0.1 (1.0-1.3)1.2 ± 0.1 (1.0-1.3)0.9 ± 0.1 (0.8-1.0)0.97 ± 0.05 (0.9-1.0)
d b 2.1 ± 0.2 (1.9-2.4)2.3 ± 0.2 (2.0-2.6)––
d’ c 1.6 ± 0.1 (1.4-1.8)1.5 ± 0.1 (1.4-1.6)––
V/Spicules length52.1 ± 2.2 (49.4-56.0)57.6 ± 0.4 (54.0-60.0)51 ± 1.5 (49.0-54.0)64.6 ± 2.5 (61.0-69.0)
Odontostyle length117.4 ± 5.5 (112.0-126.0)113.6 ± 7.4 (103.0-120.0)109.4 ± 3.6 (102.0-118.3)109.0 ± 4.7 (101.5-117.7)
Odontophore length63.5 ± 2.2 (61.0-67.0)63.4 ± 3.3 (59.0.68.0)61.2 ± 2.5 (56.0-64.0)63.0 ± 1.8 (61.0-66.5)
Total stylet length180.9 ± 6.8 (173.0-192.0)177.0 ± 6.7 (167.0-184.0)––
Anterior end to guide ring31.6 ± 1.9 (28.0-33.5)33.2 ± 1.3 (32.0-35.0)30.6 ± 2.1 (27.2-35.8)30.4 ± 1.6 (27.7-32.9)
Tail length42.9 ± 2.1 (38.0-45.0)46.6 ± 1.3 (44.0-49.0)37.8 ± 3.2 (31.5-45.0)42.4 ± 3.3 (36.4-46.3)
Hyaline part of tail length16.4 ± 1.5 (14.0-18.5)14.3 ± 2.0 (12.5-17.0)13.1 ± 1.1 (10.5-15.4)11.5 ± 1.6 (10.0-14.9)
Body width at level of
 lip region14.9 ± 0.7 (13.5-16.0)14.8 ± 1.0 (13.5-16.0)15.2 ± 0.9 (14.0-17.0)14.2 ± 0.5 (13.9-15.0)
 guide ring23.1 ± 1.5 (21-24.5)22.6 ± 1.5 (20.0-23.5)23.5 ± 1.1 (21.0-25.0)21.8 ± 1.0 (19.6-23.0)
 anus38.8 ± 1.3 (37.0-41.5)36.6 ± 1.5 (32.0-39.0)41.4 ± 2.4 (36.6-44.6)42.6 ± 2.3 (37.6-45.7)
DOI: https://doi.org/10.21307/jofnem-2021-034 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 10
Published on: Jan 1, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
Cytochrome oxidase c subunit 1,
D2-D3 of 28S rRNA,
Description,
ITS1 rRNA,
Needle nematodes,
Taxonomy
Related subjects:
Life sciences,
Life sciences, other

© 2021 Emmanuel A. Tzortzakakis, Ilenia Clavero-Camacho, Carolina Cantalapiedra-Navarrete, Parthenopi Ralli, Juan E. Palomares-Rius, Pablo Castillo, Antonio Archidona-Yuste, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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