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Effects of fluopyram and azadirachtin integration with sunn hemp on nematode communities in zucchini, tomato and sweet potato in Hawaii Cover

Effects of fluopyram and azadirachtin integration with sunn hemp on nematode communities in zucchini, tomato and sweet potato in Hawaii

Open Access
|Jan 2021

Figures & Tables

Figure 1:

Abundance of A) reniform and B) root-knot nematodes affected by treatments on zucchini or on tomato (C, D) in Trial I. Means (n-9) followed by the same letter(s) are not different according to Waller-Duncan k-ratio (k  =  100) t-test. Velum I = fluopyram treatment at crop planting; Velum II  =  fluopyram treatment at crop planting and 2 weeks after the planting; SH + Velum = pre-plant sunn hemp cover crop followed by fluopyram treatment at 1 month after crop planting; SH + MoltX = sunn hemp cover crop and monthly azadirachtin treatment; Control = untreated bare ground.
Abundance of A) reniform and B) root-knot nematodes affected by treatments on zucchini or on tomato (C, D) in Trial I. Means (n-9) followed by the same letter(s) are not different according to Waller-Duncan k-ratio (k  =  100) t-test. Velum I = fluopyram treatment at crop planting; Velum II  =  fluopyram treatment at crop planting and 2 weeks after the planting; SH + Velum = pre-plant sunn hemp cover crop followed by fluopyram treatment at 1 month after crop planting; SH + MoltX = sunn hemp cover crop and monthly azadirachtin treatment; Control = untreated bare ground.

Figure 2:

Abundance of A) reniform and B) root-knot nematodes on zucchini or on tomato (C, D) affected by treatments in Trial II. Means (n = 6) followed by the same letter(s) are not different according to Waller-Duncan k-ratio (k = 100) t-test. Velum I = fluopyram treatment at crop planting; Velum II = fluopyram treatment at 2 weeks after crop planting; SH + Velum = pre-plant sunn hemp cover crop followed by fluopyram treatment 4 weeks after planting; SH + MoltX = sunn hemp cover crop followed by monthly azadirachtin treatment; Control = untreated bare ground.
Abundance of A) reniform and B) root-knot nematodes on zucchini or on tomato (C, D) affected by treatments in Trial II. Means (n = 6) followed by the same letter(s) are not different according to Waller-Duncan k-ratio (k = 100) t-test. Velum I = fluopyram treatment at crop planting; Velum II = fluopyram treatment at 2 weeks after crop planting; SH + Velum = pre-plant sunn hemp cover crop followed by fluopyram treatment 4 weeks after planting; SH + MoltX = sunn hemp cover crop followed by monthly azadirachtin treatment; Control = untreated bare ground.

Figure 3:

Abundance of A) reniform and B) root-knot nematodes on sweet potato affected by treatments in Trial III. Means (n = 12) followed by the same letter(s) are not different according to Waller-Duncan k-ratio (k = 100) t-test. Velum I = fluopyram treatment at 0 and 3 months after crop planting; Velum II = fluopyram treatment at 2 weeks and 3 months after crop planting; SH + Velum = pre-plant sunn hemp cover crop followed by fluopyram at 1 and 3 months after crop planting; SH + MoltX = pre-plant sunn hemp cover crop and monthly azadirachtin treatment; Control = untreated bare ground.
Abundance of A) reniform and B) root-knot nematodes on sweet potato affected by treatments in Trial III. Means (n = 12) followed by the same letter(s) are not different according to Waller-Duncan k-ratio (k = 100) t-test. Velum I = fluopyram treatment at 0 and 3 months after crop planting; Velum II = fluopyram treatment at 2 weeks and 3 months after crop planting; SH + Velum = pre-plant sunn hemp cover crop followed by fluopyram at 1 and 3 months after crop planting; SH + MoltX = pre-plant sunn hemp cover crop and monthly azadirachtin treatment; Control = untreated bare ground.

Abundance (/250 cm3 of soil) of bacterivorous, fungivorous, and omnivorous nematodes on tomato affected by treatments in Trial II_

TreatmentsBacterivoresFungivoresOmnivores
Velum I68 ± 19cz 90 ± 16b7 ± 7bc
Velum II110 ± 35bc77 ± 22b5 ± 5c
SH + Velum702 ± 122a563 ± 158a35 ± 27ab
SH + MoltX1162 ± 457a657 ± 194a62 ± 30a
Control163 ± 65b62 ± 17b43 ± 25a

Abundance of bacterivorous, fungivorous, and omnivorous nematodes (/250 cm3 of soil) on tomato affected by treatments in Trial I_

TreatmentsBacterivoresFungivoresOmnivores
2 months after planting
Velum I149 ± 54d253 ± 61b3 ± 3b
Velum II393 ± 90cd410 ± 80b7 ± 3b
SH + Velum2193 ± 769a900 ± 120a40 ± 30ab
SH + MoltX1623 ± 613ab1123 ± 185a220 ± 157a
Control587 ± 148bc293 ± 18b10 ± 6b
3 months after planting
Velum I140 ± 36c103 ±  60b0 ± 0b
Velum II70 ± 25d127 ± 15b0 ± 0b
SH + Velum320 ± 76b407 ± 235a0 ± 0b
SH + MoltX817 ± 147a407 ± 235a57 ± 14a
Control370 ± 35b180 ± 104ab47 ± 27a

Abundance (/250 cm3 of soil) of bacterivorous, fungivorous, and omnivorous nematodes on zucchini affected by treatments in Trial II_

BacterivoresFungivoresOmnivores
Treatments0 week8 weeks
Velum I40 ± 31bz 353 ± 175a118 ± 41b0 ± 0a
Velum II50 ± 25b194 ± 95a95 ± 37b0 ± 0a
SH + Velum547 ± 115a510 ± 216a450 ± 96a8 ± 5a
SH + MoltX470 ± 118a547 ± 235a553 ± 113a13 ± 6a
Control53 ± 15b633 ± 111a87 ± 47b18 ± 13a

Chlorophyll content, canopy width, fruit number, and Meloidogyne-induced root-gall index on zucchini and tomato affected by treatments in Trials I and II_

TreatmentChlorophyll content (SPAD)Canopy width (cm)Root weight (g)Fruit no./plantRoot-gall index (0-10 scale)
(Trial)IIIIIIIIIIIIIII
Zucchini
Velum I36 ± 1bcz 47 ± 2a87 ± 7b114 ± 5c89 ± 12b35 ± 4b50 ± 3bc7 ± 1ab3 ± 1a2 ± 0c
Velum II37 ± 1b46 ± 1a89 ± 8b118 ± 6bc102 ± 27ab31 ± 4b40 ± 5c5 ± 1bc3 ± 1a2 ± 1bc
SH + Velum44 ± 1a47 ± 1a117 ± 4a134 ± 3a155 ± 12a49 ± 4a72 ± 3a10 ± 1a4 ± 1a3 ± 1b
SH + MoltX42 ± 2a45 ± 1a109 ± 6a127 ± 6ab124 ± 19ab39 ± 4ab54 ± 5b6 ± 1bc4 ± 2a5 ± 2a
Control34 ± 2c44 ± 1a69 ± 10c98 ± 6d71 ± 40b35 ± 4b20 ± 11d3 ± 1c7 ± 0a5 ± 2a
Tomato
Chlorophyll content (SPAD)Canopy width (cm)Root weight (g)Fruit weight g/plotRoot-gall index (0-10 scale)
Velum I44 ± 1a110 ± 10bc53 ± 9a3525 ± 717a123 ± 50a4 ± 1b2 ± 1c
Velum II43 ± 1ab95 ± 21c76 ± 17a1074 ± 471c101 ± 29a3 ± 1b4 ± 1b
SH + Velum45 ± 1a92 ± 3c87 ± 22a1995 ± 258b86 ± 32a2 ± 1b6 ± 1ab
SH + MoltX40 ± 1b153 ± 11ab64 ± 19a3460 ± 80a105 ± 45a6 ± 0ab7 ± 1a
Control43 ± 2ab164 ± 26a77 ± 17a1625 ± 604b112 ± 65a8 ± 0a8 ± 0a

Abundance (/250 cm3 of soil) of bacterivorous, fungivorous, and omnivorous nematodes on zucchini affected by treatments Trial I_

TreatmentsBacterivoresFungivoresOmnivores
Velum I614 ± 178bz 392 ± 226c21 ± 18a
Velum II827 ± 489b360 ± 208c30 ± 21a
SH + Velum1203 ± 217a722 ± 417ab23 ± 16a
SH + MoltX1538 ± 682a844 ± 488a40 ± 28a
Control871 ± 195ab416 ± 150bc23 ± 13a

Abundance (/250 cm3 of soil) of bacterivorous nematodes on sweet potato affected by treatments in Trial III_

Bacterivores
Treatments0 week2 months3 months7 monthsFungivoresOmnivores
Velum I270 ± 91abz 313 ± 55c137 ± 47bc43 ± 20b83 ± 29b1 ± 1c
Velum II310 ± 95ab690 ± 316bc117 ± 47c70 ± 12b113 ± 32b4 ± 4c
SH + Velum617 ± 292ab723 ± 20ab293 ± 48abc280 ± 86a693 ± 339a6 ± 3bc
SH + MoltX820 ± 354a1520 ± 525a803 ± 158a563 ± 206a460 ± 207a23 ± 8a
Control150 ± 111b1223 ± 308a350 ± 66ab390 ± 83a57 ± 12b19 ± 8ab

Fluopyram and azadirachtin chemigation schedules for Trials I, II, and III_

Chemigation schedule
Trial I (2017)Trial II (2018)Trial III (2019)
TreatmentsZucchini and tomatoZucchini and tomatoSweet potato
Velum IAt plantingAt plantingAt planting and 3 months post-planting
Velum IIAt planting and 2 weeks post-plantingAt 2 weeks post-plantingAt 2 weeks and 3 months post-planting
SH + VelumAt 4 weeks post-plantingAt 4 weeks post-plantingAt 1 and 3 month(s) post-planting
SH + MoltXAt monthly intervalAt monthly intervalAt monthly interval
ControlUntreated bare groundUntreated bare groundUntreated bare ground

Marketable and unmarketable sweet potato root number and weight affected by treatments in Trial III_

MarketableUnmarketable
TreatmentNumberWeight (kg)NumberWeight (kg)
Velum I2 ± 0az 0.7 ± 0.2a2 ± 1a0.4 ± 0.1a
Velum II2 ± 0a0.8 ± 0.4a2 ± 1a0.3 ± 0.1a
SH + Velum3 ± 1a1.0 ± 0.3a3 ± 1a0.7 ± 0.2a
SH + MoltX1 ± 0b0.2 ±  0.1a2 ± 0a0.7 ±  0.3a
Control0 ± 0b0.1 ± 0.1b2 ± 0a0.5 ±  0.1a
DOI: https://doi.org/10.21307/jofnem-2021-030 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 15
Published on: Jan 1, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2021 Philip Waisen, Koon-Hui Wang, Jensen Uyeda, Roxana Y. Myers, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.