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Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa Cover

Morphological and molecular characterization of Pratylenchus species from Yam (Dioscorea spp.) in West Africa

Open Access
|Jan 2021

Figures & Tables

Figure 1:

Proportion of Pratylenchus spp. in the yam rhizosphere from Ghana “n = 81” (A) and Nigeria “n = 114” (B), in yam tubers “n = 400” (C) and of nematode damage symptoms on yam tubers (D)
Proportion of Pratylenchus spp. in the yam rhizosphere from Ghana “n = 81” (A) and Nigeria “n = 114” (B), in yam tubers “n = 400” (C) and of nematode damage symptoms on yam tubers (D)

Figure 2:

Pratylenchus brachyurus. Light micrographs of Female: A: Entire body; B: Esophageal region; C: Spermatheca with sperm cells; D: Posterior end of gravid female; E: Tail end; F: Lateral field at mid body; G: Vulva; H: Tail; (scale bars: B-H = 10 μm; A = 100 μm).
Pratylenchus brachyurus. Light micrographs of Female: A: Entire body; B: Esophageal region; C: Spermatheca with sperm cells; D: Posterior end of gravid female; E: Tail end; F: Lateral field at mid body; G: Vulva; H: Tail; (scale bars: B-H = 10 μm; A = 100 μm).

Figure 3:

Morphological variations in Pratylenchus brachyurus. A-F: Anterior regions (A-F); Lateral field at mid body (G-J); Tail region (K-Q); and Tail end (R-Y); (scale bars: 10 μm).
Morphological variations in Pratylenchus brachyurus. A-F: Anterior regions (A-F); Lateral field at mid body (G-J); Tail region (K-Q); and Tail end (R-Y); (scale bars: 10 μm).

Figure 4:

Light micrographs of female Pratylenchus hexincisus. A: Anterior end; B: Entire body; C: Head; D-E: Reproductive track; F-G: Lateral field at mid body; H-I: Tail; (scale bars: A, C-I = 10 μm; B = 100 μm).
Light micrographs of female Pratylenchus hexincisus. A: Anterior end; B: Entire body; C: Head; D-E: Reproductive track; F-G: Lateral field at mid body; H-I: Tail; (scale bars: A, C-I = 10 μm; B = 100 μm).

Figure 5:

Light micrographs of female Pratylenchus zeae. A: Entire body; B: Anterior region; C: Head; D: esophageal region; E-F: Lateral field at mid body; G-H: Reproductive tract showing small round spermatheca; I-K: Tail; (scale bars: B-K, = 10 μm; A = 100 μm).
Light micrographs of female Pratylenchus zeae. A: Entire body; B: Anterior region; C: Head; D: esophageal region; E-F: Lateral field at mid body; G-H: Reproductive tract showing small round spermatheca; I-K: Tail; (scale bars: B-K, = 10 μm; A = 100 μm).

Figure 6:

Bayesian 50% majority rule consensus tree from four runs as inferred from analysis of the D2-D3 of 28 S rRNA gene sequence alignment under the GTR + I + G model. (-lnL = 11091.5259; AIC = 22563.051780; freqA = 0.1873; freqC = 0.2354; freqG = 0.3250; freqT = 0.2523; R(a) = 1.0893; R(b) = 3.9431; R(c) = 2.1703; R(d) = 0.4799; R(e) = 5.3436; R(f) = 1.0000; p-inv = 0.3210; gamma shape = 0.8480). Posterior probability values exceeding 50% are given on appropriate clades). New sequences are indicated by bold font.
Bayesian 50% majority rule consensus tree from four runs as inferred from analysis of the D2-D3 of 28 S rRNA gene sequence alignment under the GTR + I + G model. (-lnL = 11091.5259; AIC = 22563.051780; freqA = 0.1873; freqC = 0.2354; freqG = 0.3250; freqT = 0.2523; R(a) = 1.0893; R(b) = 3.9431; R(c) = 2.1703; R(d) = 0.4799; R(e) = 5.3436; R(f) = 1.0000; p-inv = 0.3210; gamma shape = 0.8480). Posterior probability values exceeding 50% are given on appropriate clades). New sequences are indicated by bold font.

Figure 7:

Bayesian 50% majority rule consensus tree from four runs as inferred from analysis of the COI mtDNA gene sequence alignment under the GTR + I + G model. (-lnL = 6250.9879; AIC = 12821.975780; freqA = 0.2952; freqC = 0.0915; freqG = 0.1808; freqT = 0.4325; R(a) = 0.1894; R(b) = 6.2295; R(c) = 1.8356; R(d) = 5.2302; R(e) = 5.3626; R(f) = 1.0000; p-inv = 0.2340; gamma shape = 0.6370). Posterior probability values exceeding 50% are given on appropriate clades). Original sequences are indicated by bold font.
Bayesian 50% majority rule consensus tree from four runs as inferred from analysis of the COI mtDNA gene sequence alignment under the GTR + I + G model. (-lnL = 6250.9879; AIC = 12821.975780; freqA = 0.2952; freqC = 0.0915; freqG = 0.1808; freqT = 0.4325; R(a) = 0.1894; R(b) = 6.2295; R(c) = 1.8356; R(d) = 5.2302; R(e) = 5.3626; R(f) = 1.0000; p-inv = 0.2340; gamma shape = 0.6370). Posterior probability values exceeding 50% are given on appropriate clades). Original sequences are indicated by bold font.

Pratylenchus spp_ recovered from yam in Ghana and Nigeria, origin, code, host, altitude and GenBank numbers_

CountryRegion*Districtα CodeSpeciesNLongitude (°)Latitude (°)Altitude (m)HostT D2-D3 COI
GhanaBrong AhafoAtebubuAtebubu PS 1 P. brachyurus 10.985987.75467151 D. rotundata T
Atebubu-AmantinAhontor 1 P. brachyurus 20.967987.79126139 D. rotundata
Tintare 2 P. brachyurus 20.904847.72486159 D. rotundata
Kintampo NorthBablioduo K 1 P. brachyurus 31.867898.0352265 D. rotundata
Kintampo S 1 P. zeae§61.840788.14824206 D. dumetorum MT362906, MT362907MT952194, MT952195
NorthernEast GonjaAdamupe 1 P. brachyurus 30.511558.49292176 D. alata MT362896MT949472
Bagabaga 1 P. brachyurus 20.613448.55865157 D. rotundata
Kitoe 1 P. brachyurus 10.495968.4655189 D. rotundata
TolonKpalsogu 2 P. brachyurus 71.013239.39844171D. rotundata
Kukuo 1 P. brachyurus 41.025339.41156171 D. rotundata
Kukuo 2 P. brachyurus 21.025569.41114170 D. rotundata
Kukuo 3 P. brachyurus 21.025569.41114170 D. rotundata
Wala 1 P. brachyurus 21.249299.63993124 D. rotundata
CountryState*LGAα CodeSpeciesNLongitude (°)Latitude (°)Altitude (m)HostT D2-D3 COI
NigeriaAbiaUmuahiaUmudike 1 P. brachyurus 17.530575.48212108 D. rotundata
Umudike 2 P. brachyurus 27.530575.48212108 D. rotundata
Umuahia NorthUmuagu 1 P. zeae 17.447395.6123490 D. dumetorum
AnambraAnambra EastIgbariam 1 P. brachyurus 16.965086.3011269 D. rotundata T
BenueOtukpoOtukpo 1 P. hexincisus 128.133277.19212196 D. alata T MT362904, KY828292MT951588, KY828320
EkitiIrepodum-IfelodumAraromi 1 P. brachyurus 25.193527.67682450 D. rotundata T
EnuguUdiAmoka 1 P. brachyurus 77.395816.55556388 D. cayenensis
ImoOwerriMbaise 1 P. brachyurus 147.034335.48433233 D. rotundata T MT362898, MT362899, MT362900, MT362901MT949474, MT949475
KogiIdahEga 1 P. brachyurus 16.729127.1012329 D. rotundata T
Ega 2 P. brachyurus 36.729127.1012329 D. rotundata T
Ega 3 P. brachyurus 16.729127.1012329 D. rotundata T
IjumuOkejumu 1 P. brachyurus 55.933387.84627495 D. rotundata T
NasarawaLafiaRimiuka 1 P. brachyurus 218.515988.49365175 D. rotundata
Rimiuka 2 P. brachyurus 68.515988.49365175 D. rotundata T
Nasarawa EggonEggon 1 P. brachyurus 88.54098.71445271 D. rotundata T MT362897

Measurements of thirteen Pratylenchus brachyurus populations from Ghana_ All measurements are in μm and in the form: mean ± s_d_ (range)_

SampleAtebubu PS 1Ahontor 1Tintare 2Bablioduo K 1Kintampo S 1Kpalsogu 2Kukuo 1
N1♀*2♀♀2♀♀3♀♀3♀♀7♀♀♀*4♀♀
L507566-543518-532556±16.9 (538-572)487±44.7 (451-537)470±38 (390-504)501±13.6 (488-520)
a16.524.0-21.018.9-21.924.4±5.4 (19.6-30.2)18.2±0.72 (17.4-18.7)16.7±2.3 (14.1-21.1)19.9±3.3 (15.8-22.6)
b6.24.6-4.65.3-5.75.8±0.64 (5.3-6.2)5.2±0.9 (4.2-5.8)4.7±0.21 (4.5-4.8)5.2±1.2 (4.2-6.9)
b'4.33.8-4.14.2-4.74.1±1.0 (3.4-5.3)3.7±0.74 (3.1-4.5)3.5±0.07 (3.4-3.5)4.1±0.62 (3.4-4.8)
C21.617.1-17.220.6-17.624.7±4.3 (19.9-28.3)16.4±1.1 (15.3-17.5)16.7±2.0 (14.5-19.5)17.4±1.3 (15.7-18.7)
c'1.52.8-1.71.6-2.01.6±0 (1.6-1.6)1.9±0.21 (1.7-2.1)1.8±0.33 (1.4-2.3)1.8±0.27 (1.6-2.2)
V%84.886.0-83.082.0-83.085.9±0.17 (85.7-86.0)85.7±0.58 (85.0-86.0)83.5±3.3 (77.0-88.0)84.3±1.5 (82-85)
Stylet length19.119.6-18.818.1-18.219.2±0.2 (19.0-19.4)18.3±0.12 (18.2-18.4)17.1±0.64 (16.6-18.4)19.1±0.68 (18.5-20.1)
Stylet knob width5.75.2-4.15.3-5.74.4±0 (4.4-4.4)4.9±0.9 (4.0-5.8)5.2±0.7 (4.3-6.3)5.2±0.51 (4.6-5.8)
Stylet knob height3.83.1-3.03.9-3.32.9±0.57 (2.5-3.3)3.0±0.65 (2.3-3.6)3.6±0.54 (2.8-4.2)3.5±0.35 (3.1-3.8)
DEGO from stylet base3.72.8-3.32.6-3.13.6±0.51 (3.2-4.2)3.8±0.64 (3.1-4.2)2.4±0.57 (2.0-2.8)2.7±0.4 (2.3-3.1)
Anterior end to:
 centre of metacorpus53.572.4-62.550.8-48.256.7±4.9 (52.4-62.1)52.7±4.6 (49.2-57.9)51.0±6.1 (42.9-61.7)54.9±6.4 (47.6-63.1)
 median bulb base61.580.7-69.259.2-55.863.6±3.6 (61.2-67.8)61.4±4.4 (57.8-66.3)57.2±7.2 (46.3-69.2)61.1±6.9 (53.4-70)
 Cardia81.1123-11797.1-93.798.4±9.1 (92.0-105)94.6±12.4 (83.2-108)103±9.4 (96.8-110)101±21.6 (72.6-124)
 end of esophageal gland end117147-134125-113140±28.1 (108-160)135±17.5 (119-154)139±5.1 (135-142)126±22.0 (103-154)
 secretory/excretory pore71.3111-87.571.6-79.491.7±8.2 (85.4-101)72.4±3.1 (70.2-74.6)72.1±9.0 (56.9-79.8)85.5±4.1 (81.6-89.8)
 Esophagus overlap51.121.6-18.327.7-18.938.3±10.0 (31.2-45.3)37.8±6.3 (31.6-44.2)39.7±0.64 (39.2-40.1)29.6±8.3 (24.1-41.7)
Max. body diam.30.723.6-25.827.4-24.223.4±4.7 (18.9-28.3)26.7±1.9 (25.3-28.8)28.5±3.1 (23.8-34.2)25.7±4.4 (22.0-30.9)
Vulval body diam.24.219.0-21.422.6-20.621.7±3.8 (18.6-25.9)21.4±1.2 (20.2-22.5)22.6±2.7 (20.4-27.3)21.4±1.8 (19.3-23.7)
Anal body diam.15.611.8-18.816.2-14.814.6±3.0 (12.4-18.0)15.6±0.61 (15.1-16.3)15.5±2.1 (11.8-18)16.1±1.6 (14.5-18.1)
Anterior genital 178-169167-186±79.8 (126-277)124±11.1 (116-132)86.1±23.1 (71.8-121)
Spermatheca-vagina -35.743.3-63.9±28.4 (45.9-96.6)37.4±9.1 (30.9-43.8)32±11.4 (24.6-45.2)
Tail length23.533.1-31.625.2-30.123.0±4.3 (20.2-28.0)29.8±4.5 (27-35)28.4±3.3 (24.4-32.6)28.9±2.4 (26-31.7)
Number of tail annuli1519.0-18.015.0-19.018.5±2.1 (17-20)18.0±2 (16.0-20.0)18.0±0.82 (17.0-19.0)17.3±1.7 (15-19)
Vulva to anus distance49.757.0-57.564.4-58.742.5±6.0 (37.7-49.2)45.9±7.6 (39.4-58.9)51.1±3.3 (47.5-55.4)
Post-uterine sac33.220.1-22.624.7-24.922.3±4.2 (17.6-25.7)21.2±4.4 (16.5-25.1)17.1±2.8 (13.6-19.5)
Lateral field width11.3-11.910.7-10.37.4±0.51 (6.7-7.9)
SampleKukuo 2Kukuo 3Wala 1Bagabaga 1Adamupe 1Kitoe 1
N4♀♀2♀♀2♀♀2♀♀3♀♀*1♀*
L541±46.1 (501-607)488-541516-560522-516549±60.1 (480-590)549
a20.3±2.6 (18.7-24.2)20.3-23.5-23.718.1-19.118.9±4.6 (15.4-24.1)16.0
b5.3±0.29 (4.9-5.6)5.0-4.85.1-5.34.9±1.8 (2.9-6.3)5.6
b'3.8±0.29 (3.5-4.1)3.9-3.94.0-4.24.1±1.5 (2.5-5.4)4.1
c17.1±2.4 (14.3-20)16.7-19.717.3-16.418.7-20.517.0±1.7 (15.8-19.0)25.1
c'2.0±0.19 (1.7-2.1)1.8-1.62.5-2.31.7-1.51.9±0.36 (1.6-2.3)1.5
V%84.5±1 (83.0-85.0)85.0-84.085.0-83.085.0-83.085.0±1.2 (84.2-86.4)85.7
Stylet length18.7±0.21 (18.4-18.9)19.3-18.219.4-20.019.3-19.818.9±0.49 (18.3-19.2)19.8
Stylet knob width5.6±0.7 (5-6.6)4.3-5.25.2-4.36.1-6.05.6±0.25 (5.3-5.8)4.9
Stylet knob height3.6±0.22 (3.4-3.9)3.7-3.93.2-3.23.7-3.73.5±0.46 (3.2-4)3.7
DEGO from stylet base3.1±0.25 (2.8-3.4)4.0-3.24.3-2.92.7±0.55 (2.1-3.1)3.0
Anterior end to:
 centre of metacorpus58.1±4.8 (53.2-62.6)52.1-55.762.4-61.655.6-53.062.5±0.98 (61.4-63.3)61.2
 median bulb base65.9±4.8 (61.6-71.2)57.2-61.471.3-72.164.8-61.270.6±0.57 (70.1-71.2)67.3
 Cardia102±7.0 (92.5-108)104-116102-96.7121±37.6 (91.3-164)97.6
 end of esophageal gland end142±9.3 (129-150)133-144130-122144±42.5 (108-191)133
 secretory/excretory pore87.6±7.5 (78.1-96.3)99.9-89.981.0-70.690.5±10.7 (79.3-101)
 Esophagus overlap36.2±3.8 (31.1-39.5)35.9-38.439.0-29.925.3±9.4 (18.0-35.9)38.0
Max. body diam.27.1±4.9 (20.7-32.4)24.0-21.9-23.628.8-2729.7±4.7 (24.5-33.5)34.2
Vulval body diam.21.9±2.6 (18.5-24.9)20.1-22.318.1-20.722.2-22.824.4±3.0 (21.0-26.4)20.6
Anal body diam.16.4±1.2 (14.8-17.5)16.2-17.612.0-15.116.6-16.817.3±1.2 (16.4-18.6)14.9
Anterior genital180±42.2 (133-234)219-210193±90.6 (129-257)
Spermatheca-vagina49.4±14.3 (37.9-65.4)38.3-42.0
Tail length31.9±3.1 (29.8-36.5)29.2-27.429.8-3427.9-25.232.5±4.3 (29.6-37.4)21.9
Number of tail annuli18.5±2.4 (16.0-21.0)16.0-15.018.0-16.017.0-16.021.7±3.2 (18.0-24.0)
Vulva to anus distance52.8±6.7 (43.0-58.2)44.4-51.748.1-59.154.3-55.749.9±9.7 (42.8-61)51.5
Post-uterine sac21.1±1.9 (19.7-23.9)18.1-19.231.5-23.918.9±1.7 (17.2-20.5)49.5
Lateral field width10.2±0.71 (9.6-11.0)11.8-10.611.5±1.8 (10.2-12.7)

Measurements of thirteen Pratylenchus brachyurus populations from Nigeria_

SampleIgbariam 1Araromi 1Okejumu 1Ega 1Ega 2Ega 3Rimiuka 2
N1♀2♀♀5♀♀1♀3♀♀1♀6♀♀
L461563–584493±55.9 (435–563)470472±33.9 (445–510)536545±30.2 (507–579)
a15.722.6–19.316.6±0.72 (15.7–17.4)16.618.0±3.1 (15.0–21.2)21.019.8±1.5 (18.6–22.7)
b
b’3.83.9–3.93.7±0.59 (2.9–4.2)3.35.04.2±0.22 (3.9–4.4)
C21.820.1–17.319.8±4.7 (15.0–26.6)22.019.6±6.1 (14.3–26.3)21.724.1±5.6 (19.6–34.9)
c’1.51.7–1.91.5±0.34 (0.93–1.8)1.61.9±0.47 (1.5–2.4)1.71.6±0.21 (1.2–1.8)
V%85.087.0–85.085.6±1.1 (84.0–87.0)85.084.7±0.58 (84–85)86.084.7±1.2 (83–86)
Stylet length17.120.4–20.418.9±0.63 (18.0–19.6)19.419.3±0.64 (18.6–19.7)19.118.6±0.44 (18.1–19.1)
Stylet knob width4.25.8–5.85.0±0.78 (3.8–5.9)4.74.9±0.51 (4.3–5.3) 5.2±0.54 (4.4–5.8)
Stylet knob height3.43.8–4.03.1±0.51 (2.5–3.7)2.93.1±0.0 (3.1–3.1) 3.3±0.21 (3.1–3.6)
DEGO from stylet base 2.8–2.92.9±0.24 (2.6–3.1)3.3 2.7±0.99 (2–3.4)
Anterior end to:
 centre of metacorpus52.461.5–64.261.9±11.1 (47.3–74.1)58.052.2±3.6 (48.4–55.6)54.658.8±7.3 (51.2–69.3)
 median bulb base58.271.3–72.769.9±10.6 (56.7–81.8)66.859.1±3.6 (55.6–62.8)62.066.4±6.7 (58–75.6)
 cardia
 end of esophageal gland end120146–151135±18.4 (110–150)144107132±8.6 (123–143)
 secretory/excretory pore63.997.0–98.189.3±12.1 (77.3–110)87.579.6±5.9 (75.4–83.8)81.390.6±10.0 (84.1–111)
 Esophagus overlap21.135.5–38.636.4±4.8 (32.9–43.2)31.121.828.9±4.3 (23–32.9)
Max. body diam.29.324.9–30.228.7±1.6 (27.4–30.9)28.326.6±4.3 (21.7–29.6)25.527.7±1.5 (25.5–29.2)
Vulval body diam.21.522.1–24.123.4±1.5 (21–24.7)21.320.8±3.7 (16.6–23.5)20.622.9±1.2 (21.3–24.6)
Anal body diam.13.716.6–17.818.0±2.9 (15.6–22.8)13.513.6±2.5 (11.3–16.3)14.814.7±0.9 (13.4–15.8)
Anterior genital
Spermatheca-vagina
Tail length21.128.0–33.825.6±4.3 (21.2–32.2)21.325.8±7.9 (16.9–32.2)24.723.4±3.7 (16.1–25.9)
Number of tail annuli
Vulva to anus distance42.747.0–53.844.3±6.5 (39.2–53.5)49.248.8±9.8 (37.6–55.9)47.459.1±8.6 (49.0–72.7)
Post-uterine sac 20.9–20.218.0±1.6 (16.4–19.9)19.9 16.6±2.8 (14.6–18.6)
Lateral field width
SampleEggon 1 Umudike 1Umudike 2Amoka 1Rimiuka 1Mbaise 1
N4♀♀*4♀♀1♀2♀♀7♀♀21♀♀14♀♀
L568 ± 57 (515–648)578±56.1 (519–648)486551–599591±42.5 (556–679)509±84.1 (394–641)569±31.4 (510–613)
a20.7±3.8 (18.6–26.3)18.7±1.4 (17.4–20.6)15.623.4–24.021.7±2.5 (18.3–24.7)20.3±2.9 (16.0–24.8)22.6±2.8 (17.0–27.0)
b3.04.7–4.85.0±0.6 (4.3–5.8)7.5±0.93 (6.3–8.8)
b’4.8±0.57 (4.1–5.5)2.54.4–4.34.0±0.48 (3.3–4.5)3.5±0.52 (2.7–4.5)4.4±0.59 (3.6–5.5)
C25.1±9.3 (19.5–39)23.6±2.2 (21.3–25.5)16.426.2–20.620.4±6.3 (15.8–34.1)18.7±3.0 (13.4–24.4)19.8±1.6 (17.9–23.2)
c’1.6±0.33 (1.2–2.0)1.5±0.13 (1.3–1.6)1.81.3–1.81.9±0.21 (1.6–2.3)1.7±0.18 (1.5–2.1)2.0±0.37 (1.5–2.5)
V%85.5±0.58 (85.0–86.0)85.0±0.82 (84.0–86.0)83.686.0–84.085.6±0.53 (85.0–86.0)84.9±1.7 (81.0–87.0)84.6±1 (82–86)
Stylet length17.8±1.0 (16.3–18.4)18.8±0.57 (18.3–19.6)19.120.5–18.519.1±0.6 (18.3–20.1)19.4±1.0 (17.2–20.9)18.9±0.69 (17.8–19.8)
Stylet knob width5.3±0.49 (4.7–5.6)4.8±0.46 (4.1–5.1)5.65.1–5.55.6±0.28 (5.0–5.8)5.0±0.46 (4.1–6.0)5.1±0.52 (4.3–5.7)
Stylet knob height3.4±0.21 (3.2–3.6)3.6±0.18 (3.4–3.8)4.03.4–3.43.9±0.45 (3.2–4.5)3.0±0.52 (2.0–3.7)3.5±0.46 (2.8–4.6)
DEGO from stylet base3.6±0.49 (3.2–3.9)3.0±0.45 (2.6–3.6)2.12.4–3.33.2±0.56 (2.4–3.9)3.2±0.51 (2.4–4.2)3.0±0.47 (2.3–3.7)
Anterior end to:
 centre of metacorpus58.0±7.1 (50.6–67.0)59.4±5.3 (55.7–67)63.358.9–61.362.9±1.5 (60.0–65.2)64.0±4.7 (54–72.6)61.7±5.1 (55.5–72.9)
 median bulb base66.0±8.0 (57.4–76.5)67.8±5.9 (63.2–75.9)71.266.2–69.070.7±1.7 (68.7–73.8)72.4±4.2 (62.2–78.4)70.5±4.7 (65.1–81.3)
 Cardia164118–125120±9.5 (104–131)78.2±9.1 (68.9–91.3)
 end of esophageal gland end122±4.7 (117–127)191127–139149±11.4 (134–167)148±17.1 (113–181)130±15.3 (103–149)
 secretory/excretory pore102±3.5 (98.8–106)84.4±13.7 (74.2–100)91.587.1–98.996.4±5.5 (89.3–102)96.9±10.3 (82.8–128)93.6±11.6 (76.5–114)
 Esophagus overlap29.2±1.9 (27.8–30.5)27.5±2.5 (24.1–29.5)35.940.9–18.826.8±7.8 (17.6–39.6)37.9±9.5 (12.6–50.6)30.0±8.7 (14.6–45.9)
Max. body diam.27.8±2.5 (24.6–30.1)31.0±2.5 (28.3–34.2)31.123.5–24.927.5±2.6 (24.5–31.2)25.3±3.5 (21.6–33.4)25.7±4.3 (21.8–34.2)
Vulval body diam.23.4±1.6 (21.4–25.2)24.2±1.6 (22.4–26)25.920.2–19.423.1±2.3 (20.3–26.3)22.1±2.8 (17.4–28.0)20.7±3.5 (16.6–28.2)
Anal body diam.15.9±3.4 (11.4–18.6)16.7±1.2 (15.8–18.4)16.916.0–16.616.0±3.2 (10.0–19.5)16.0±1.8 (12.6–18.7)14.8±2.6 (11.2–19.3)
Anterior genital129141–198166±66.1 (84.3–243)206±43.6 (162–261)
Spermatheca-vagina24.237.5–39.437.5±16.2 (20.1–55.0)51.1±25.7 (17.1–77.6)
Tail length24.7±7.7 (13.2–29.7)24.5±2.2 (21.6–26.7)29.621.0–29.130.6±6.9 (17.7–37.4)27.3±2.2 (23.1–32)28.8±2.5 (25–32.1)
Number of tail annuli18.016.0–21.9±1.5 (20.0–24.0)23.8±2.2 (21–27)
Vulva to anus distance60.0±7.8 (50.3–66.9)57.7±8.8 (51.2–70.7)45.960.9–65.752.9±11.4 (42.8–73.2)48.5±9.1 (25.9–62.9)58.9±3.7 (54.2–66.7)
Post-uterine sac19.5±2.9 (16.2–21.5)19.112.3–14.219.9±3.0 (17.2–25.1)18.9±4.7 (14.3–34.9)19.8±1.8 (16.1–22.8)
Lateral field width12.78.4–7.79.4±1.6 (7–10.8)7.3±1.1 (6.1–8.1)

Measurements of two Pratylenchus zeae populations from Ghana and Nigeria_

SampleUmuagu 1Kintampo S 1
n1♀2♀♀*4♀♀
L382381–561433±36.9 (403–483)
a23.117.5–17.419.9±2.7 (17.1–23.5)
b 4.5±0.36 (4.0–4.8)
b’ 4.5±1.3 (3.2–6.3)
c28.715.6–21.717.6±2.1 (14.7–19.7)
c’1.81.8–1.72.0±0.24 (1.6–2.1)
V%70.370.0–70.871.8±1.1 (70.7–73.2)
Stylet length15.714.6–14.715.9±0.95 (14.6–16.9)
Stylet knob width 4.9–4.74.1±0.4 (3.7–4.4)
Stylet knob height 2.9–3.32.4±0.29 (2.1–2.7)
DEGO from stylet base3.52.9±0.21 (2.6–3.1)
Anterior end to:
 centre of metacorpus44.147.4–50.3±3.1 (47.3–53.7)
 median bulb base 55.9–57.6±3.3 (53.9–60.7)
 cardia 95.7±8.3 (84.2–103)
 end of esophageal gland end116101±24.1 (69.6–126)
 secretory/excretory pore69.172.0–71.1±7.9 (64.8–82.6)
 Esophagus overlap 21.4–23.922.3±4.3 (17.1–26.5)
Max. body diam.16.521.8–32.321.9±1.4 (20.5–23.8)
Vulval body diam.15.320.8–24.219.6±1.2 (18.3–20.7)
Anal body diam.7.213.3–15.012.9±0.89 (12.0–14.1)
Anterior genital 159±27.5 (121–183)
Spermatheca-vagina 36.3±3.5 (31.6–40.2)
Tail length13.324.5–25.924.8±3.2 (20.4–27.7)
Number of tail annuli 19.3±1.5 (18.0–21.0)
Vulva to anus distance11093.2–14994.2±7.4 (85.6–103)
Post-uterine sac25.819.6–30.421.5±5.3 (15.3–26.5)
Lateral field width 7.9±1.2 (6.5–9.3)

Measurements of a Pratylenchus hexincisus population from Nigeria_

SampleOtukpo 1
n5♀♀*7♀♀
L503±99 (367–625)427±34.7 (382–492)
a18.4±3.1 (16.4–22)22.4±1.4 (20.8–24.6)
b6.1±0.52 (5.6–7)
b’4.4±0.71 (3.8–5.6)
c15.9±3.5 (13.5–19.9)12.8±1.9 (10.8–15.7)
c’2.4±0.31 (2.1–2.7)2.6±0.42 (2.2–3.4)
V%75.7±1.6 (74.1–78)74.9±1.8 (72.6–78)
Stylet length13.9±0.43 (13.6–14.5)14.9±1.6 (11.8–16.1)
Stylet knob width4.3±0.27 (3.9–4.5)2.1±0.19 (2.0–2.4)
Stylet knob height2.6±0.28 (2.3–2.9)2.4±0.21 (2.2–2.6)
DEGO from stylet base3.3±0.34 (2.8–3.6)4.4±0.87 (3.8–5.4)
Anterior end to:
 centre of metacorpus50.0±4.1 (46.8–57.0)45.5±5.8 (36.0–50.7)
 median bulb base57.4±3.6 (54.4–63.5)54.3±4.3 (48.5–58.8)
 cardia70.6±7.5 (59.2–81.1)
 end of esophageal gland end101±17.2 (74.4–124)
 secretory/excretory pore54.7±3.9 (50.0–59.2)
 Esophagus overlap
Max. body diam.24.3±2.2 (22.3–26.6)19.2±1.7 (17.4–22.4)
Vulval body diam.20.9±4.8 (17.4–30.3)
Anal body diam.14.6±0.51 (14–15.2)13.1±1.8 (11.0–15.2)
Anterior genital93.7±17.5 (81.3–106)
Spermatheca-vagina
Tail length36.1±4.1 (31.4–39.0)34.0±4.7 (28.8–39.7)
Number of tail annuli24.0±2.8 (22.0–26.0)
Vulva to anus distance
Post-uterine sac
Lateral field width7.0±0.28 (6.8–7.2)
DOI: https://doi.org/10.21307/jofnem-2020-126 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 25
Published on: Jan 22, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2021 Yao A. Kolombia, Oluwadamilola Ogundero, Emmanuel Olajide, Nicole Viaene, P. Lava Kumar, Danny L. Coyne, Wim Bert, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.