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Morphological and molecular characterization of Hoplolaimus pararobustus (Schuurmans Stekhoven and Teunissen, 1938) Sher 1963 with its first report on Zea mays roots in Namibia

Open Access
|Jan 2021

Figures & Tables

Figure 1:

Hoplolaimus pararobustus. Female 1: Anterior part of the body; 2: Oesophageal overlap with an excretory pore; 3: Vulval area with posterior spermatheca; 4: Phasmid and lateral field in the anterior part of the body; 5: Tail with the lateral field; Male: 6: Phasmid with the lateral field at the posterior end; 7: Posterior end with spicules and bursa; 8: Anterior part of the body; 9: Lateral field opposite vulva. Scale bar = 20 µm.
Hoplolaimus pararobustus. Female 1: Anterior part of the body; 2: Oesophageal overlap with an excretory pore; 3: Vulval area with posterior spermatheca; 4: Phasmid and lateral field in the anterior part of the body; 5: Tail with the lateral field; Male: 6: Phasmid with the lateral field at the posterior end; 7: Posterior end with spicules and bursa; 8: Anterior part of the body; 9: Lateral field opposite vulva. Scale bar = 20 µm.

Figure 2:

Bayesian tree inferred from partial 18 S rDNA sequence of Hoplolaimus pararobustus obtained from the rhizosphere of maize from Namibia under GTR + I + G (partition = 010020; lnL = 3717.3614; rAC = 1.0000; rAG = 2.0466; rAT = 1.0000; rCG = 1.0000; rCT = 5.6942; rGT = 1.0000; p-inv = 0.4370; gama shape = 0.3390) based on 50% majority role. The newly obtained sequence is indicated by bold font.
Bayesian tree inferred from partial 18 S rDNA sequence of Hoplolaimus pararobustus obtained from the rhizosphere of maize from Namibia under GTR + I + G (partition = 010020; lnL = 3717.3614; rAC = 1.0000; rAG = 2.0466; rAT = 1.0000; rCG = 1.0000; rCT = 5.6942; rGT = 1.0000; p-inv = 0.4370; gama shape = 0.3390) based on 50% majority role. The newly obtained sequence is indicated by bold font.

Figure 3:

Bayesian tree inferred from partial 28 S rDNA sequence of Hoplolaimus pararobustus obtained from the rhizosphere of maize from Namibia under GTR + G (partition = 012314; lnL = 5311.5111; rAC = 0.9205; rAG = 4.5584; rAT = 2.2804; rCG = 0.4018; rCT = 4.5584; rGT = 1.0000; gamma shape = 0.2940) based on 50% majority role. The newly obtained sequence is indicated by bold font.
Bayesian tree inferred from partial 28 S rDNA sequence of Hoplolaimus pararobustus obtained from the rhizosphere of maize from Namibia under GTR + G (partition = 012314; lnL = 5311.5111; rAC = 0.9205; rAG = 4.5584; rAT = 2.2804; rCG = 0.4018; rCT = 4.5584; rGT = 1.0000; gamma shape = 0.2940) based on 50% majority role. The newly obtained sequence is indicated by bold font.

Measurements of Hoplolaimus pararobustus females and males from maize in Namibia_

Hoplolaimus pararobustus (Namibian specimens) Hoplolaimus pararobustus (acc. to Fortuner (1991) Hoplolaimus pararobustus (acc. to Van den Berg and Quénéhervé, 2012) Hoplolaimus columbus (acc. to Fortuner, 1991) Hoplolaimus dubius (acc. to Chaturvedi and Khera, 1979; Zarina and Maqbool, 1998) Hoplolaimus galeatus (acc. to Van den Berg and Quénéhervé, 2012) Hoplolaimus seinhorsti (acc. to Van den Berg and Quénéhervé, 2012 Hoplolaimus seinhorsti (acc. to Mansourabad et al., 2016)
CharactersFemalesMalesFemalesMalesFemalesMalesFemalesMalesFemalesMalesFemalesMales
n21211714 208 8
L1,100±76.1 (957–1,245)925±65 (818–1,018)1,314±0.127 (910–1,800)1,158±0.102 (930–1,500)940–1,800920–1,5001,260–18001,150–1,400780–1,600940–1,1001,100–1,9401,050–1,5601,000–1,6001,480–1,738
a29±3.5 (23.7–36)29.2±4.2 (22–38.3)27.3±2.689 (20–39)29.2±2.2227 (21–37.2)20–3921–37.230–3831.9 (25.9–39.2)31.94–51.2932.78–43.8722–3423–3224.1–4025.5–33.8
b7.4±0.7 (6.2–8.6)6.7±0.4 (6–7.5)8.98±1.361 (6–14)8.5±1.328 (6.2–13.8)6–14.16.2–13.89.1–12.410.9 (9.58–12.18)8.16–12.498.0–9.456.3–10.88.3–10.35.2–14.2
b’9.5±0.8 (7.9–11)8.5±0.7 (7.1–10.1)7.1±0.682 (5.1–10)6.6±0.57 (5–8.7)6.3–9.7 6.04–9.425.95–6.76
c59.7±10.5 (46.9–80.6)34.7±4.3 (25–41)60.8±15.185 (10–164)36.9±6.605 (22.2–51.9)40–16422.2–51.939–5729.9 (26.8–33.1)1.321.4135.08–41.210.6–0.71.5–1.738–74
c’0.8±0.1 (0.6–1.1)1.5±0.2 (1.3–2)0.67±0.135 (0.4–0.9)1.6±0.206 (1.4–2.1)0.4–0.91.4–2.1 0.61–1.17 0.7–0.9
o (%)13±2 (8–17)13±2.5 (8–18)6.8–13.47.1–12.59–135.2 (4.8–5.2)7.55–16.2812.5–17.78.5–1710–17
M51±1.5 (48–54)51±1.8 (50–53)
V (%)55±2.5 (49–67)52.68±2.609 (51–62) 51–62 51–60 41.56–59.16 52–60
OV1 (%)31±14.0 (18–51)
OV2 (%)
h13±2.5 (10–19)
Annulus width2±0.3 (2–3)2±0.3 (2–3)2 2–3
Lip region height7±0.5 (6–8)6±0.6 (5–7)
Lip region width13±1 (12–15)12±0.9 (11–13)
Stylet length36±1.6 (34–40)34±1.3 (32–38)42.3±2.378 (37.5–49)40.8±2.673 (35–46)37.5–49.735–4840–4842 (40.2–43.7)34.4–42.4 41.5–5440–4838–45
Metenchium length18±1.1 (17–21)17±0.6 (16–18)
Telenchium length18±0.8 (17–19)17±1 (15–20)
Stylet knob height6±0.5 (5–7)5±0.6 (4–6)
Stylet knob width6±0.8 (5–9)6±0.5 (5–7) 6.0–6.8
Dorsal gland opening posterior to stylet knobs5±0.7 (3–6)4±0.9 (3–6)5.6±1.377 (4–8) 3.2–5.6
Median bulb length17±1.3 (15–20)16±1.5 (13–19)
Median bulb width15±1.3 (13–17)12±1 (11–15)
Medan bulb valve length6±0.7 (4–6)5±0.7 (4–7)
Median bulb valve width4±0.6 (3–5)4±0.4 (3–4)
Excretory pore from anterior end108±11.9 (82–123)93±13.6 (74–121)
Esophagus length148±7.6 (136–161)139±10.4 (124–163)
Esophagus overlap31±7.2 (15–44)30±5.7 (20–38)
Body width at excretory pore33±3.5 (27–40)26±2.4 (23–30)
Body width at midbody39±4 (32–46)32±2.9 (27–38)
Body width at anus/cloaca25±2.2 (21–29)17±1.3 (15–20)
Position of excretory pore/Esophagus length (%)73±7.5 (61–85)67±9.6 (61–85)
Position of excretory pore/Body length (%)10±1.3 (8–12)10±(8–13)
Anterior genital track length241±69.2 (171–301)
Testes length350±34.6 (322–400) (n=4)
Spicule length38±2.3 (35–42) 46.9±3.318 (40–57) 40–57 46.8 (36.6–52.5) 368–43.36 40–52
Gubernaculum length17±2.3 (11–20) 21.9±2.4296 (15.4–31) 15.4–31 14.4–18.4 20–28
Capitulum length11 ±0.8 (10–13)
Phasmid diameter4±0.3 (3–4)3±0.5 (3–4) 5.3 (4.1–6)
Anterior phasmid as % from anterior end36±6.9 (28–40) (n=3)36±3.5 (28–43) 22–5223.7–50.429–4738 (35.4–42.2)23.90–41.3421.18–44.3523–4629–4524–46
Posterior phasmid as % from anterior end tail79±4.1 (70–85)81±4.7 (72–87) 58–8968.9–85.479–9082 (79.7–83.2)82.20–96.4476.69–85.9972–8875–8968–90
Tail length19±3.1 (15–24)27±3.5 (22–35) 7–1516–2826.1–35.1 17.5–36.232.3–44.222–28
Number of tail annules 12–18
DOI: https://doi.org/10.21307/jofnem-2020-124 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 1 - 12
Published on: Jan 13, 2021
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year

© 2021 Mariette Marais, Esther van den Berg, Hendrika Fourie, Milad Rashidifard, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.